Restriction map for
pFN21ASDB0027
(Circular) (Six-base) MAPSORT of: pFN21ASDB0027.seq Check: 3910 from: 1 to: 7666
pFN21ASDB0027 7666 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3444 5476 278
Size: 53 83 186 936 1908 2032 2468
Fragments arranged by size:
2468 2032 1908 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4113 4959 5009 1937
Size: 2176 846 50 4594
Fragments arranged by size:
4594 2176 846 50
Acc65I G'GTAC_C
Cuts at: 4987 4987
Size: 7666
AclI AA'CG_TT
Cuts at: 1563 3105 5793 6166 1563
Size: 1542 2688 373 3063
Fragments arranged by size:
3063 2688 1542 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5729 7113 7381 1632
Size: 4097 1384 268 1917
Fragments arranged by size:
4097 1917 1384 268
AfeI AGC'GCT
Cuts at: 2260 4121 2260
Size: 1861 5805
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7469
AflIII A'CryG_T
Cuts at: 1203 4629 4842 6565 7542 1203
Size: 3426 213 1723 977 1327
Fragments arranged by size:
3426 1723 1327 977 213
AgeI A'CCGG_T
Cuts at: 6497 6497
Size: 7666
AhdI GACnn_n'nnGTC
Cuts at: 2359 6395 2359
Size: 4036 3630
AleI CACnn'nnGTG
Cuts at: 1166 4133 1166
Size: 2967 4699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2970 3563 1184
Size: 1786 593 5287
Fragments arranged by size:
5287 1786 593
AlwNI CAG_nnn'CTG
Cuts at: 2326 2338 2917 3431 3628 3982 5409 6981 2326
Size: 12 579 514 197 354 1427 1572 3011
Fragments arranged by size:
3011 1572 1427 579 514 354 197 12
ApaI G_GGCC'C
Cuts at: 2041 2911 2041
Size: 870 6796
ApaLI G'TGCA_C
Cuts at: 4681 5723 6879 4681
Size: 1042 1156 5468
Fragments arranged by size:
5468 1156 1042
ApoI r'AATT_y
Cuts at: 1493 3409 4975 5201 5353 7462 1493
Size: 1916 1566 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 1566 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6220 160
Size: 872 5188 1606
Fragments arranged by size:
5188 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7666
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2672 2978 3515 4762 4991 1870
Size: 73 146 583 306 537 1247 229 4545
Fragments arranged by size:
4545 1247 583 537 306 229 146 73
BamHI G'GATC_C
Cuts at: 2028 4996 5363 2028
Size: 2968 367 4331
Fragments arranged by size:
4331 2968 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3554 3586 3835 3926 4430
Size: 325 217 627 1767 32 249 91 504
Cuts at: 4430 4648 4987 6442 618
Size: 218 339 1455 1842
Fragments arranged by size:
1842 1767 1455 627 504 339 325 249
218 217 91 32
BanII G_rGCy'C
Cuts at: 1391 2041 2096 2224 2247 2746 2911 4017 4110
Size: 650 55 128 23 499 165 1106 93
Cuts at: 4110 4349 4823 4985 1391
Size: 239 474 162 4072
Fragments arranged by size:
4072 1106 650 499 474 239 165 162
128 93 55 23
BbeI G_GCGC'C
Cuts at: 3558 3558
Size: 7666
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2769 3448 4032 4148 928
Size: 971 870 679 584 116 4446
Fragments arranged by size:
4446 971 870 679 584 116
BbvCI CC'TCA_GC
Cuts at: 3564 3845 3564
Size: 281 7385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 5857 5891 1903
Size: 34 2795 34 1091 34 3678
Fragments arranged by size:
3678 2795 1091 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5558 6768 5558
Size: 1210 6456
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7666
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2219 3116
Size: 107 122 71 631 605 538 9 897
Cuts at: 3116 4478 4623 6277 136
Size: 1362 145 1654 1525
Fragments arranged by size:
1654 1525 1362 897 631 605 538 145
122 107 71 9
BglII A'GATC_T
Cuts at: 2754 7661 2754
Size: 4907 2759
BlpI GC'TnA_GC
Cuts at: 3345 3424 3674 4232 5083 3345
Size: 79 250 558 851 5928
Fragments arranged by size:
5928 851 558 250 79
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2911 3007 3591 3840 4433 4637 4685
Size: 251 870 96 584 249 593 204 48
Cuts at: 4685 5727 6883 1790
Size: 1042 1156 2573
Fragments arranged by size:
2573 1156 1042 870 593 584 251 249
204 96 48
BmgBI CAC'GTC
Cuts at: 1318 4686 1318
Size: 3368 4298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3700 5397 6355
Size: 416 53 83 399 24 2283 1697 958
Cuts at: 6355 442
Size: 1753
Fragments arranged by size:
2283 1753 1697 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7666
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4471 4738 4471
Size: 267 7399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3830 4070 6326 1239
Size: 2591 240 2256 2579
Fragments arranged by size:
2591 2579 2256 240
Bpu10I CC'TnA_GC
Cuts at: 3564 3600 3845 4152 4179 3564
Size: 36 245 307 27 7051
Fragments arranged by size:
7051 307 245 36 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 2492 3134 3230 3707 4001 4913 5791 6656
Size: 45 642 96 477 294 912 878 865
Cuts at: 6656 6954 2447
Size: 298 3159
Fragments arranged by size:
3159 912 878 865 642 477 298 294
96 45
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2238 2245 4359 6329 882
Size: 617 739 7 2114 1970 2219
Fragments arranged by size:
2219 2114 1970 739 617 7
BsaAI yAC'GTr
Cuts at: 493 1204 4264 4630 4963 5457 493
Size: 711 3060 366 333 494 2702
Fragments arranged by size:
3060 2702 711 494 366 333
BsaBI GATnn'nnATC
Cuts at: 3197 7660 3197
Size: 4463 3203
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2790 3441 3555 3918
Size: 53 83 186 936 1257 651 114 363
Cuts at: 3918 5473 5855 6490 275
Size: 1555 382 635 1451
Fragments arranged by size:
1555 1451 1257 936 651 635 382 363
186 114 83 53
BsaWI w'CCGG_w
Cuts at: 2862 6099 6497 6771 6918 7329 2862
Size: 3237 398 274 147 411 3199
Fragments arranged by size:
3237 3199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4043 4073 1178
Size: 30 165 30 2640 30 4771
Fragments arranged by size:
4771 2640 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2590 3275 3494 4727 1186
Size: 1404 685 219 1233 4125
Fragments arranged by size:
4125 1404 1233 685 219
BseYI C'CCAG_C
Cuts at: 2255 2685 3550 4170 6869 7365 2255
Size: 430 865 620 2699 496 2556
Fragments arranged by size:
2699 2556 865 620 496 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2411 3417 3808 4157 4550 4796 1163
Size: 573 675 1006 391 349 393 246 4033
Fragments arranged by size:
4033 1006 675 573 393 391 349 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2110 4618 5116 5877 6905 1540
Size: 275 186 109 2508 498 761 1028 2301
Fragments arranged by size:
2508 2301 1028 761 498 275 186 109
BsiHKAI G_wGCw'C
Cuts at: 2949 3425 4017 4349 4685 5727 5812 6883 2949
Size: 476 592 332 336 1042 85 1071 3732
Fragments arranged by size:
3732 1071 1042 592 476 336 332 85
BsmI GAATG_Cn'
Cuts at: 2625 5177 5270 2625
Size: 2552 93 5021
Fragments arranged by size:
5021 2552 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2096 2224 2247 2746 2911 2949
Size: 399 251 55 128 23 499 165 38
Cuts at: 2949 3007 3425 3591 3840 4017 4110 4349 4433
Size: 58 418 166 249 177 93 239 84
Cuts at: 4433 4637 4685 4823 4985 5727 5812 6883 1391
Size: 204 48 138 162 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 418 399 251 249
239 204 177 166 165 162 138 128
93 85 84 58 55 48 38 23
BspEI T'CCGG_A
Cuts at: 7329 7329
Size: 7666
BspHI T'CATG_A
Cuts at: 2814 4209 4293 5555 7285 2814
Size: 1395 84 1262 1730 3195
Fragments arranged by size:
3195 1730 1395 1262 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2323 2706 3603 3909 5021 844
Size: 1479 383 897 306 1112 3489
Fragments arranged by size:
3489 1479 1112 897 383 306
BsrBI CCG'CTC
Cuts at: 1394 1823 3349 3654 5553 1394
Size: 429 1526 305 1899 3507
Fragments arranged by size:
3507 1899 1526 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 3558 4298 6161 6335 65
Size: 1183 2310 740 1863 174 1396
Fragments arranged by size:
2310 1863 1396 1183 740 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6310 6497 1700
Size: 110 4500 187 2869
Fragments arranged by size:
4500 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4164 96
Size: 4068 3598
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7666
BssSI C'ACGA_G
Cuts at: 3338 5726 6738 3338
Size: 2388 1012 4266
Fragments arranged by size:
4266 2388 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 2338 794
Size: 1532 12 6122
Fragments arranged by size:
6122 1532 12
BstBI TT'CG_AA
Cuts at: 2007 6470 2007
Size: 4463 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7666
BstYI r'GATC_y
Cuts at: 1972 2028 2754 2859 4225 4996 5363 5748 5765
Size: 56 726 105 1366 771 367 385 17
Cuts at: 5765 7217 7303 7315 7661 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1366 771 726 385 367 346
105 86 56 17 12
Bsu36I CC'TnA_GG
Cuts at: 2215 4332 4691 2215
Size: 2117 359 5190
Fragments arranged by size:
5190 2117 359
BtgI C'CryG_G
Cuts at: 513 1065 2208 2298 4732 513
Size: 552 1143 90 2434 3447
Fragments arranged by size:
3447 2434 1143 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2607 2626 2860 3643 4812 487
Size: 683 56 1381 19 234 783 1169 3341
Fragments arranged by size:
3341 1381 1169 783 683 234 56 19
BtsI GCAGTG_nn'
Cuts at: 2845 3329 3799 4586 5182 5976 5996 2845
Size: 484 470 787 596 794 20 4515
Fragments arranged by size:
4515 794 787 596 484 470 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7666
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7631
DraI TTT'AAA
Cuts at: 4970 4970
Size: 7666
DraIII CAC_nnn'GTG
Cuts at: 3694 3694
Size: 7666
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3866 6673 809
Size: 507 2550 2807 1802
Fragments arranged by size:
2807 2550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2202 3141
Size: 54 1425 50 275 18 277 95 939
Cuts at: 3141 3933 4068 4242 4615 5113 6002 7631 8
Size: 792 135 174 373 498 889 1629 43
Fragments arranged by size:
1629 1425 939 889 792 498 373 277
275 174 135 95 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2107 4615 5113 1537
Size: 275 295 2508 498 4090
Fragments arranged by size:
4090 2508 498 295 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3512 3810 4043 5596 1353
Size: 2159 298 233 1553 3423
Fragments arranged by size:
3423 2159 1553 298 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2021 2157 2165 6253 6627 6773
Size: 1064 138 432 136 8 4088 374 146
Cuts at: 6773 387
Size: 1280
Fragments arranged by size:
4088 1280 1064 432 374 146 138 136
8
EcoICRI GAG'CTC
Cuts at: 4015 4347 4015
Size: 332 7334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3830 4070 5729 6326 7113 7381 1239
Size: 393 2198 240 1659 597 787 268 1524
Fragments arranged by size:
2198 1659 1524 787 597 393 268 240
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 3245 4244 2330
Size: 915 999 5752
Fragments arranged by size:
5752 999 915
EcoO109I rG'GnC_Cy
Cuts at: 2038 2132 2323 2335 2802 3084 4033 4249 2038
Size: 94 191 12 467 282 949 216 5455
Fragments arranged by size:
5455 949 467 282 216 191 94 12
EcoRI G'AATT_C
Cuts at: 4975 5353 4975
Size: 378 7288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3322 3661 4054 3322
Size: 339 393 6934
Fragments arranged by size:
6934 393 339
FspI TGC'GCA
Cuts at: 6172 6172
Size: 7666
HaeII r_GCGC'y
Cuts at: 1678 2262 2918 3558 4123 6813 1678
Size: 584 656 640 565 2690 2531
Fragments arranged by size:
2690 2531 656 640 584 565
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2585 2617 3383
Size: 32 135 32 773 32 208 32 766
Cuts at: 3383 3415 4043 4075 4135 4167 6307 6339 6381
Size: 32 628 32 60 32 2140 32 42
Cuts at: 6381 6413 1373
Size: 32 2626
Fragments arranged by size:
2626 2140 773 766 628 208 135 60
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4759 5010 5256 669
Size: 988 281 2821 251 246 3079
Fragments arranged by size:
3079 2821 988 281 251 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7666
HpaI GTT'AAC
Cuts at: 5256 5256
Size: 7666
KasI G'GCGC_C
Cuts at: 3554 3554
Size: 7666
KpnI G_GTAC'C
Cuts at: 4991 4991
Size: 7666
MfeI C'AATT_G
Cuts at: 5265 5265
Size: 7666
MluI A'CGCG_T
Cuts at: 7542 7542
Size: 7666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3426 3612 6780 6964 1305
Size: 2121 186 3168 184 2007
Fragments arranged by size:
3168 2121 2007 186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4070 10
Size: 54 1425 343 2238 3606
Fragments arranged by size:
3606 2238 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3545 4133 4502 4742 5624 5983
Size: 648 2037 342 588 369 240 882 359
Cuts at: 5983 6142 518
Size: 159 2042
Fragments arranged by size:
2042 2037 882 648 588 369 359 342
240 159
MspA1I CmG'CkG
Cuts at: 1606 2124 2210 2344 3428 4236 4273 4734 4924
Size: 518 86 134 1084 808 37 461 190
Cuts at: 4924 5082 5759 6907 7152 1606
Size: 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 1084 808 677 518 461 245
190 158 134 86 37
NarI GG'CG_CC
Cuts at: 3555 3555
Size: 7666
NcoI C'CATG_G
Cuts at: 513 1065 2298 513
Size: 552 1233 5881
Fragments arranged by size:
5881 1233 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7666
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7666
NotI GC'GGCC_GC
Cuts at: 5113 5113
Size: 7666
NspI r_CATG'y
Cuts at: 1132 1229 3055 3076 3577 3718 4318 4501 4846
Size: 97 1826 21 501 141 600 183 345
Cuts at: 4846 5024 6569 1132
Size: 178 1545 2229
Fragments arranged by size:
2229 1826 1545 600 501 345 183 178
141 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7666
PciI A'CATG_T
Cuts at: 4842 6565 4842
Size: 1723 5943
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2304 3694 4645 1108
Size: 1196 1390 951 4129
Fragments arranged by size:
4129 1390 1196 951
PfoI T'CCnGG_A
Cuts at: 3459 3756 3855 3459
Size: 297 99 7270
Fragments arranged by size:
7270 297 99
PmeI GTTT'AAAC
Cuts at: 4970 4970
Size: 7666
PmlI CAC'GTG
Cuts at: 4264 4264
Size: 7666
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5735 5767 7248 7280 5735
Size: 32 1481 32 6121
Fragments arranged by size:
6121 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2132 2335 3084 4033 2132
Size: 203 749 949 5765
Fragments arranged by size:
5765 949 749 203
PshAI GACnn'nnGTC
Cuts at: 1613 2464 1613
Size: 851 6815
PsiI TTA'TAA
Cuts at: 4851 5236 4851
Size: 385 7281
PspOMI G'GGCC_C
Cuts at: 2037 2907 2037
Size: 870 6796
PstI C_TGCA'G
Cuts at: 830 2331 2343 2664 2751 2835 2852 3081 3794
Size: 1501 12 321 87 84 17 229 713
Cuts at: 3794 4083 4662 5018 830
Size: 289 579 356 3478
Fragments arranged by size:
3478 1501 713 579 356 321 289 229
87 84 17 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7666
PvuII CAG'CTG
Cuts at: 2124 2344 4273 2124
Size: 220 1929 5517
Fragments arranged by size:
5517 1929 220
SacI G_AGCT'C
Cuts at: 4017 4349 4017
Size: 332 7334
SacII CC_GC'GG
Cuts at: 2211 4735 2211
Size: 2524 5142
SalI G'TCGA_C
Cuts at: 1936 5008 1936
Size: 3072 4594
SanDI GG'GwC_CC
Cuts at: 2132 2132
Size: 7666
SbfI CC_TGCA'GG
Cuts at: 5018 5018
Size: 7666
ScaI AGT'ACT
Cuts at: 3221 5914 7626 3221
Size: 2693 1712 3261
Fragments arranged by size:
3261 2693 1712
SexAI A'CCwGG_T
Cuts at: 4548 4548
Size: 7666
SfcI C'TryA_G
Cuts at: 826 1045 2327 2339 2660 2747 2831 2848 3077
Size: 219 1282 12 321 87 84 17 229
Cuts at: 3077 3790 3942 4079 4658 4803 5014 6149 6830
Size: 713 152 137 579 145 211 1135 681
Cuts at: 6830 7021 826
Size: 191 1471
Fragments arranged by size:
1471 1282 1135 713 681 579 321 229
219 211 191 152 145 137 87 84
17 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2210 2210
Size: 7666
SfoI GGC'GCC
Cuts at: 3556 3556
Size: 7666
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7666
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7666
SmaI CCC'GGG
Cuts at: 1872 4764 4993 1872
Size: 2892 229 4545
Fragments arranged by size:
4545 2892 229
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 2507 3113 3245 3722 4016
Size: 197 926 519 45 606 132 477 294
Cuts at: 4016 4928 5770 6671 6933 820
Size: 912 842 901 262 1553
Fragments arranged by size:
1553 926 912 901 842 606 519 477
294 262 197 132 45
SnaBI TAC'GTA
Cuts at: 493 4963 5457 493
Size: 4470 494 2702
Fragments arranged by size:
4470 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7666
SphI G_CATG'C
Cuts at: 1132 3577 4318 4501 5024 1132
Size: 2445 741 183 523 3774
Fragments arranged by size:
3774 2445 741 523 183
SspI AAT'ATT
Cuts at: 5 52 2929 5590 5
Size: 47 2877 2661 2081
Fragments arranged by size:
2877 2661 2081 47
StuI AGG'CCT
Cuts at: 1517 2164 3119 3501 3644 3821 1517
Size: 647 955 382 143 177 5362
Fragments arranged by size:
5362 955 647 382 177 143
StyI C'CwwG_G
Cuts at: 513 1065 2298 2649 3645 4676 5105 513
Size: 552 1233 351 996 1031 429 3074
Fragments arranged by size:
3074 1233 1031 996 552 429 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3511 3682 4184 4244 4594 4666 4804
Size: 1255 1931 171 502 60 350 72 138
Cuts at: 4804 5693 5710 5863 6048 6493 325
Size: 889 17 153 185 445 1498
Fragments arranged by size:
1931 1498 1255 889 502 445 350 185
171 153 138 72 60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3069 3219 4164
Size: 274 80 33 51 1443 1092 150 945
Cuts at: 4164 5912 7624 96
Size: 1748 1712 138
Fragments arranged by size:
1748 1712 1443 1092 945 274 150 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3426 3458 7626 7658 3426
Size: 32 4168 32 3434
Fragments arranged by size:
4168 3434 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7666
XbaI T'CTAG_A
Cuts at: 5002 5002
Size: 7666
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3928 4588 4618 3928
Size: 660 30 6976
Fragments arranged by size:
6976 660 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7666
XmaI C'CCGG_G
Cuts at: 1870 4762 4991 1870
Size: 2892 229 4545
Fragments arranged by size:
4545 2892 229
XmnI GAAnn'nnTTC
Cuts at: 2891 5795 2891
Size: 2904 4762
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3442 5474 276
Size: 53 83 186 936 1908 2032 2468
Fragments arranged by size:
2468 2032 1908 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SapI SrfI SwaI