Restriction map for
pFN21ASDB0012
(Circular) (Six-base) MAPSORT of: pFN21ASDB0012.seq Check: 7195 from: 1 to: 6160
pFN21ASDB0012 6160 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2045 2045
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3503 1937
Size: 1566 4594
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2715 3275 3413 4223 5607 5875 1632
Size: 1083 560 138 810 1384 268 1917
Fragments arranged by size:
1917 1384 1083 810 560 268 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AlwNI CAG_nnn'CTG
Cuts at: 2474 2981 3903 5475 2474
Size: 507 922 1572 3159
Fragments arranged by size:
3159 1572 922 507
ApaI G_GGCC'C
Cuts at: 2086 2086
Size: 6160
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 3047 3469 3695 3847 5956 1493
Size: 1554 422 226 152 2109 1697
Fragments arranged by size:
2109 1697 1554 422 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 2539 3485 1870
Size: 73 596 946 4545
Fragments arranged by size:
4545 946 596 73
BamHI G'GATC_C
Cuts at: 3490 3857 3490
Size: 367 5793
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2103 2461 2762 3185 3481
Size: 325 217 627 316 358 301 423 296
Cuts at: 3481 4936 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 423 358 325 316 301
296 217
BanII G_rGCy'C
Cuts at: 1391 2086 2216 3307 3479 1391
Size: 695 130 1091 172 4072
Fragments arranged by size:
4072 1091 695 172 130
BbeI G_GCGC'C
Cuts at: 2465 2465
Size: 6160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3401 928
Size: 971 1502 3687
Fragments arranged by size:
3687 1502 971
BbvCI CC'TCA_GC
Cuts at: 2742 3117 2742
Size: 375 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2683 4052 5262 2683
Size: 1369 1210 3581
Fragments arranged by size:
3581 1369 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2138 2321 2432
Size: 107 122 71 631 605 466 183 111
Cuts at: 2432 4771 136
Size: 2339 1525
Fragments arranged by size:
2339 1525 631 605 466 183 122 111
107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2086 4221 5377 1790
Size: 296 2135 1156 2573
Fragments arranged by size:
2573 2135 1156 296
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2138 2384 3891
Size: 416 53 83 399 24 721 246 1507
Cuts at: 3891 4849 442
Size: 958 1753
Fragments arranged by size:
1753 1507 958 721 416 399 246 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2546 4820 1239
Size: 1085 222 2274 2579
Fragments arranged by size:
2579 2274 1085 222
Bpu10I CC'TnA_GC
Cuts at: 2742 3117 2742
Size: 375 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 4285 5150 5448 2264
Size: 2021 865 298 2976
Fragments arranged by size:
2976 2021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2529 4823 882
Size: 617 1030 2294 2219
Fragments arranged by size:
2294 2219 1030 617
BsaAI yAC'GTr
Cuts at: 493 1204 3457 3951 493
Size: 711 2253 494 2702
Fragments arranged by size:
2702 2253 711 494
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2290 2462 3967 4349
Size: 53 83 186 936 757 172 1505 382
Cuts at: 4349 4984 275
Size: 635 1451
Fragments arranged by size:
1505 1451 936 757 635 382 186 172
83 53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2544 2574 2901 2931 1178
Size: 30 165 30 1141 30 327 30 4407
Fragments arranged by size:
4407 1141 327 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2216 2621 2633 2636 2744 3134 1186
Size: 1027 3 405 12 3 108 390 4212
Fragments arranged by size:
4212 1027 405 390 108 12 3 3
BseYI C'CCAG_C
Cuts at: 2436 2467 5363 5859 2436
Size: 31 2896 496 2737
Fragments arranged by size:
2896 2737 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2212 2339 1163
Size: 573 476 127 4984
Fragments arranged by size:
4984 573 476 127
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2533 3610 4371 5399 1540
Size: 275 186 532 1077 761 1028 2301
Fragments arranged by size:
2301 1077 1028 761 532 275 186
BsiHKAI G_wGCw'C
Cuts at: 4221 4306 5377 4221
Size: 85 1071 5004
Fragments arranged by size:
5004 1071 85
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
BsmBI CGTCTCn'nnnn_
Cuts at: 2637 2637
Size: 6160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2216 3307 3479 4221 4306 5377
Size: 399 296 130 1091 172 742 85 1071
Cuts at: 5377 1391
Size: 2174
Fragments arranged by size:
2174 1091 1071 742 399 296 172 130
85
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 2124 3515 844
Size: 1201 79 1391 3489
Fragments arranged by size:
3489 1391 1201 79
BsrBI CCG'CTC
Cuts at: 1394 1823 3332 4047 1394
Size: 429 1509 715 3507
Fragments arranged by size:
3507 1509 715 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 4655 4829 65
Size: 1183 1458 1949 174 1396
Fragments arranged by size:
1949 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4804 4991 1700
Size: 110 2994 187 2869
Fragments arranged by size:
2994 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2983 96
Size: 2887 3273
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2046 2235 2934 794
Size: 1252 189 699 4020
Fragments arranged by size:
4020 1252 699 189
BstBI TT'CG_AA
Cuts at: 2007 4964 2007
Size: 2957 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 3490 3857 4242 4259 5711 5797 5809 6155
Size: 1518 367 385 17 1452 86 12 346
Cuts at: 6155 1972
Size: 1977
Fragments arranged by size:
1977 1518 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2502 2518 3162 3276 513
Size: 552 957 480 16 644 114 3397
Fragments arranged by size:
3397 957 644 552 480 114 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3048 3185 487
Size: 683 56 1822 137 3462
Fragments arranged by size:
3462 1822 683 137 56
BtsI GCAGTG_nn'
Cuts at: 2331 3676 4470 4490 2331
Size: 1345 794 20 4001
Fragments arranged by size:
4001 1345 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3231 3266 529
Size: 35 2667 35 3423
Fragments arranged by size:
3423 2667 35 35
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2217 2412
Size: 54 1425 50 275 18 300 87 195
Cuts at: 2412 2499 2515 3607 4496 6125 8
Size: 87 16 1092 889 1629 43
Fragments arranged by size:
1629 1425 1092 889 300 275 195 87
87 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2730 3026 4090 1353
Size: 1269 108 296 1064 3423
Fragments arranged by size:
3423 1269 1064 296 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2137 4747 5121 5267 387
Size: 1064 138 548 2610 374 146 1280
Fragments arranged by size:
2610 1280 1064 548 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2546 2715 3275 3413 4223 4820
Size: 393 692 222 169 560 138 810 597
Cuts at: 4820 5607 5875 1239
Size: 787 268 1524
Fragments arranged by size:
1524 810 787 692 597 560 393 268
222 169 138
EcoO109I rG'GnC_Cy
Cuts at: 2523 2632 3103 2523
Size: 109 471 5580
Fragments arranged by size:
5580 471 109
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 6160
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2465 2474 5307 1678
Size: 787 9 2833 2531
Fragments arranged by size:
2833 2531 787 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3064 3096 4801 4833 4875
Size: 32 135 32 1492 32 1705 32 42
Cuts at: 4875 4907 1373
Size: 32 2626
Fragments arranged by size:
2626 1705 1492 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3504 3750 669
Size: 988 281 1566 246 3079
Fragments arranged by size:
3079 1566 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KasI G'GCGC_C
Cuts at: 2461 2461
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 10
Size: 54 1425 343 300 4038
Fragments arranged by size:
4038 1425 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2997 4118 4477 4636 518
Size: 648 1831 1121 359 159 2042
Fragments arranged by size:
2042 1831 1121 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2185 3576 4253 5401 5646 1606
Size: 474 105 1391 677 1148 245 2120
Fragments arranged by size:
2120 1391 1148 677 474 245 105
NarI GG'CG_CC
Cuts at: 2462 2462
Size: 6160
NcoI C'CATG_G
Cuts at: 513 1065 2022 3162 513
Size: 552 957 1140 3511
Fragments arranged by size:
3511 1140 957 552
NdeI CA'TA_TG
Cuts at: 387 2994 387
Size: 2607 3553
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2077 3358 3518 5063 1132
Size: 97 848 1281 160 1545 2229
Fragments arranged by size:
2229 1545 1281 848 160 97
PasI CC'CwG_GG
Cuts at: 1348 3307 1348
Size: 1959 4201
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2759 1108
Size: 1651 4509
PfoI T'CCnGG_A
Cuts at: 2163 2163
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2523 2632 3103 2523
Size: 109 471 5580
Fragments arranged by size:
5580 471 109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PspOMI G'GGCC_C
Cuts at: 2082 2082
Size: 6160
PstI C_TGCA'G
Cuts at: 830 3512 830
Size: 2682 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SanDI GG'GwC_CC
Cuts at: 2523 2523
Size: 6160
SapI GCTCTTCn'nnn_
Cuts at: 3026 3026
Size: 6160
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 3288 4408 6120 3288
Size: 1120 1712 3328
Fragments arranged by size:
3328 1712 1120
SfcI C'TryA_G
Cuts at: 826 1045 3508 4643 5324 5515 826
Size: 219 2463 1135 681 191 1471
Fragments arranged by size:
2463 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2138 2432 2138
Size: 294 5866
SfoI GGC'GCC
Cuts at: 2463 2463
Size: 6160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 4264 5165 5427 820
Size: 197 926 336 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 3457 3951 493
Size: 2964 494 2702
Fragments arranged by size:
2964 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6160
StyI C'CwwG_G
Cuts at: 513 1065 2022 2758 3162 3599 513
Size: 552 957 736 404 437 3074
Fragments arranged by size:
3074 957 736 552 437 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2547 4187 4204 4357 4542 4987 325
Size: 1255 967 1640 17 153 185 445 1498
Fragments arranged by size:
1640 1498 1255 967 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2983 3286 3297
Size: 274 80 33 51 1443 1006 303 11
Cuts at: 3297 4406 6118 96
Size: 1109 1712 138
Fragments arranged by size:
1712 1443 1109 1006 303 274 138 80
51 33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2267 2299 2364 2396 2544 2576 6120 6152 2267
Size: 32 65 32 148 32 3544 32 2275
Fragments arranged by size:
3544 2275 148 65 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2494
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 2963 3171 4289 2963
Size: 208 1118 4834
Fragments arranged by size:
4834 1118 208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SexAI SrfI SwaI