Restriction map for
pFN21ASDB0008
(Circular) (Six-base) MAPSORT of: pFN21ASDB0008.seq Check: 8779 from: 1 to: 6622
pFN21ASDB0008 6622 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3965 1937
Size: 2028 4594
Acc65I G'GTAC_C
Cuts at: 3344 3943 3344
Size: 599 6023
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2787 2949 3113 4685 6069 6337 1632
Size: 1155 162 164 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 1155 268 164 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 5521 6498 1203
Size: 4318 977 1327
Fragments arranged by size:
4318 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 2472 5351 2472
Size: 2879 3743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 2389 2788 3178 4365 5937 2389
Size: 399 390 1187 1572 3074
Fragments arranged by size:
3074 1572 1187 399 390
ApaLI G'TGCA_C
Cuts at: 3554 4679 5835 3554
Size: 1125 1156 4341
Fragments arranged by size:
4341 1156 1125
ApoI r'AATT_y
Cuts at: 1493 2109 3227 3931 4157 4309 6418 1493
Size: 616 1118 704 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 704 616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 3134 3687 3947 1870
Size: 73 1191 553 260 4545
Fragments arranged by size:
4545 1191 553 260 73
BamHI G'GATC_C
Cuts at: 3952 4319 3952
Size: 367 6255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3344 3943 5398 618
Size: 325 217 627 1557 599 1455 1842
Fragments arranged by size:
1842 1557 1455 627 599 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 2337 3941 1391
Size: 730 216 1604 4072
Fragments arranged by size:
4072 1604 730 216
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 3101 3381 928
Size: 971 572 630 280 4169
Fragments arranged by size:
4169 971 630 572 280
BbvCI CC'TCA_GC
Cuts at: 2682 2682
Size: 6622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4813 4847 1903
Size: 34 2876 34 3678
Fragments arranged by size:
3678 2876 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2461 3176 4514 5724 2461
Size: 715 1338 1210 3359
Fragments arranged by size:
3359 1338 1210 715
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 5233 136
Size: 107 122 71 631 605 366 3195 1525
Fragments arranged by size:
3195 1525 631 605 366 122 107 71
BglII A'GATC_T
Cuts at: 6617 6617
Size: 6622
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4683 5839 1790
Size: 1768 1125 1156 2573
Fragments arranged by size:
2573 1768 1156 1125
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2449 4353 5311
Size: 416 53 83 399 24 1032 1904 958
Cuts at: 5311 442
Size: 1753
Fragments arranged by size:
1904 1753 1032 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3676 3676
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2351 3310 5282 1239
Size: 896 216 959 1972 2579
Fragments arranged by size:
2579 1972 959 896 216
Bpu10I CC'TnA_GC
Cuts at: 2682 2682
Size: 6622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4747 5612 5910 4747
Size: 865 298 5459
Fragments arranged by size:
5459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 3780 5285 882
Size: 617 561 1720 1505 2219
Fragments arranged by size:
2219 1720 1505 617 561
BsaAI yAC'GTr
Cuts at: 493 1204 3305 3919 4413 493
Size: 711 2101 614 494 2702
Fragments arranged by size:
2702 2101 711 614 494
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4429 4811 5446 275
Size: 53 83 186 936 2896 382 635 1451
Fragments arranged by size:
2896 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5055 5453 5727 5874 6285 5055
Size: 398 274 147 411 5392
Fragments arranged by size:
5392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6397
Fragments arranged by size:
6397 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 2834 3263 3878 1186
Size: 1417 231 429 615 3930
Fragments arranged by size:
3930 1417 615 429 231
BseYI C'CCAG_C
Cuts at: 2132 2958 5825 6321 2132
Size: 826 2867 496 2433
Fragments arranged by size:
2867 2433 826 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6049
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4072 4833 5861 1540
Size: 275 186 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 3187 3301 3558 4683 4768 5839 2121
Size: 1066 114 257 1125 85 1071 2904
Fragments arranged by size:
2904 1125 1071 1066 257 114 85
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
BsmBI CGTCTCn'nnnn_
Cuts at: 2834 2834
Size: 6622
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2337 3187 3301 3558 3941 4683
Size: 399 331 216 850 114 257 383 742
Cuts at: 4683 4768 5839 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 850 742 399 383 331 257
216 114 85
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 3367 3649 4511 6241 3367
Size: 282 862 1730 3748
Fragments arranged by size:
3748 1730 862 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3977 844
Size: 3133 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2439 4509 1394
Size: 429 616 2070 3507
Fragments arranged by size:
3507 2070 616 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3366 5117 5291 65
Size: 1183 2118 1751 174 1396
Fragments arranged by size:
2118 1751 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5266 5453 1700
Size: 110 3456 187 2869
Fragments arranged by size:
3456 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2547 96
Size: 2451 4171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 2741 4682 5694 2741
Size: 1941 1012 3669
Fragments arranged by size:
3669 1941 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6622
BstBI TT'CG_AA
Cuts at: 2007 5426 2007
Size: 3419 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6622
BstYI r'GATC_y
Cuts at: 1972 2343 3952 4319 4704 4721 6173 6259 6271
Size: 371 1609 367 385 17 1452 86 12
Cuts at: 6271 6617 1972
Size: 346 1977
Fragments arranged by size:
1977 1609 1452 385 371 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2481 2481
Size: 6622
BtgI C'CryG_G
Cuts at: 513 1065 2022 2844 3669 513
Size: 552 957 822 825 3466
Fragments arranged by size:
3466 957 825 822 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2951 487
Size: 683 56 1725 4158
Fragments arranged by size:
4158 1725 683 56
BtsI GCAGTG_nn'
Cuts at: 3558 4138 4932 4952 3558
Size: 580 794 20 5228
Fragments arranged by size:
5228 794 580 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2826 2861 529
Size: 35 2262 35 4290
Fragments arranged by size:
4290 2262 35 35
DraI TTT'AAA
Cuts at: 3232 3926 3232
Size: 694 5928
DraIII CAC_nnn'GTG
Cuts at: 3305 3305
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587
Size: 54 1425 50 275 18 2239 889 1629
Cuts at: 6587 8
Size: 43
Fragments arranged by size:
2239 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2412 2763 3018 3512 3778 4552 1353
Size: 1059 351 255 494 266 774 3423
Fragments arranged by size:
3423 1059 774 494 351 266 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5209 5583 5729 387
Size: 1064 138 3620 374 146 1280
Fragments arranged by size:
3620 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2119 2119
Size: 6622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2351 2787 2949 3113 3310 4685
Size: 393 503 216 436 162 164 197 1375
Cuts at: 4685 5282 6069 6337 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1375 787 597 503 436 393 268
216 197 164 162
EcoRI G'AATT_C
Cuts at: 3931 4309 3931
Size: 378 6244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2771 3428 3592 2771
Size: 657 164 5801
Fragments arranged by size:
5801 657 164
FspI TGC'GCA
Cuts at: 3003 5128 3003
Size: 2125 4497
HaeII r_GCGC'y
Cuts at: 1678 5769 1678
Size: 4091 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2458 2490 2591 2623 2897
Size: 32 135 32 886 32 101 32 274
Cuts at: 2897 2929 3001 3033 5263 5295 5337 5369 1373
Size: 32 72 32 2230 32 42 32 2626
Fragments arranged by size:
2626 2230 886 274 135 101 72 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2103 3966 4212 669
Size: 988 281 165 1863 246 3079
Fragments arranged by size:
3079 1863 988 281 246 165
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6622
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KpnI G_GTAC'C
Cuts at: 3348 3947 3348
Size: 599 6023
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2540 5736 5920 1305
Size: 1235 3196 184 2007
Fragments arranged by size:
3196 2007 1235 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4800
Fragments arranged by size:
4800 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4580 4939 5098 518
Size: 648 3414 359 159 2042
Fragments arranged by size:
3414 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2132 2329 2765 2927 4038 4715 5863 6108
Size: 526 197 436 162 1111 677 1148 245
Cuts at: 6108 1606
Size: 2120
Fragments arranged by size:
2120 1148 1111 677 526 436 245 197
162
NcoI C'CATG_G
Cuts at: 513 1065 2022 3669 513
Size: 552 957 1647 3466
Fragments arranged by size:
3466 1647 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 3980 5525 1132
Size: 97 2751 1545 2229
Fragments arranged by size:
2751 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2034 1348
Size: 686 5936
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 2857 3808 1108
Size: 1245 504 951 3922
Fragments arranged by size:
3922 1245 951 504
PfoI T'CCnGG_A
Cuts at: 2058 2058
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PmlI CAC'GTG
Cuts at: 3305 3305
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 6590
PstI C_TGCA'G
Cuts at: 830 3974 830
Size: 3144 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 2132 2329 2765 2927 2132
Size: 197 436 162 5827
Fragments arranged by size:
5827 436 197 162
SacI G_AGCT'C
Cuts at: 2121 2121
Size: 6622
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SapI GCTCTTCn'nnn_
Cuts at: 2763 3018 2763
Size: 255 6367
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 3806 4870 6582 3806
Size: 1064 1712 3846
Fragments arranged by size:
3846 1712 1064
SfcI C'TryA_G
Cuts at: 826 1045 2403 2721 3170 3263 3431 3970 5105
Size: 219 1358 318 449 93 168 539 1135
Cuts at: 5105 5786 5977 826
Size: 681 191 1471
Fragments arranged by size:
1471 1358 1135 681 539 449 318 219
191 168 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3689 3949 1872
Size: 1817 260 4545
Fragments arranged by size:
4545 1817 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3134 4726 5627 5889 820
Size: 197 926 1191 1592 901 262 1553
Fragments arranged by size:
1592 1553 1191 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3919 4413 493
Size: 3426 494 2702
Fragments arranged by size:
3426 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 3911 1517
Size: 2394 4228
StyI C'CwwG_G
Cuts at: 513 1065 2022 2306 3669 3894 4061 513
Size: 552 957 284 1363 225 167 3074
Fragments arranged by size:
3074 1363 957 552 284 225 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 2414 4649 4666 4819 5004 5449
Size: 1255 707 127 2235 17 153 185 445
Cuts at: 5449 325
Size: 1498
Fragments arranged by size:
2235 1498 1255 707 445 185 153 127
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 3804 4868
Size: 274 80 33 51 1443 570 1257 1064
Cuts at: 4868 6580 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1257 1064 570 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6582 6614 6582
Size: 32 6590
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XhoI C'TCGA_G
Cuts at: 1943 3134 1943
Size: 1191 5431
XmaI C'CCGG_G
Cuts at: 1870 3687 3947 1870
Size: 1817 260 4545
Fragments arranged by size:
4545 1817 260
XmnI GAAnn'nnTTC
Cuts at: 3854 4751 3854
Size: 897 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI