Restriction map for
pFN21ASDB0005
(Circular) (Six-base) MAPSORT of: pFN21ASDB0005.seq Check: 6214 from: 1 to: 8542
pFN21ASDB0005 8542 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5343 6352 278
Size: 53 83 186 936 3807 1009 2468
Fragments arranged by size:
3807 2468 1009 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2748 4869 5835 5885 1937
Size: 811 2121 966 50 4594
Fragments arranged by size:
4594 2121 966 811 50
Acc65I G'GTAC_C
Cuts at: 5863 5863
Size: 8542
AclI AA'CG_TT
Cuts at: 1563 6669 7042 1563
Size: 5106 373 3063
Fragments arranged by size:
5106 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2810 4025 4172 4484 4507 5345 5426 6605
Size: 1178 1215 147 312 23 838 81 1179
Cuts at: 6605 7989 8257 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1215 1179 1178 838 312 268
147 81 23
AfeI AGC'GCT
Cuts at: 2132 2180 2132
Size: 48 8494
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8345
AflIII A'CryG_T
Cuts at: 1203 4554 7441 8418 1203
Size: 3351 2887 977 1327
Fragments arranged by size:
3351 2887 1327 977
AgeI A'CCGG_T
Cuts at: 7373 7373
Size: 8542
AhdI GACnn_n'nnGTC
Cuts at: 5725 7271 5725
Size: 1546 6996
AleI CACnn'nnGTG
Cuts at: 1166 3233 1166
Size: 2067 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2111 4032 1184
Size: 927 1921 5694
Fragments arranged by size:
5694 1921 927
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 4061 4093 3023
Size: 32 1006 32 7472
Fragments arranged by size:
7472 1006 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2125 3772 4150 4666 4784 4936 5050 5064 5323
Size: 1647 378 516 118 152 114 14 259
Cuts at: 5323 5758 6285 7857 2125
Size: 435 527 1572 2810
Fragments arranged by size:
2810 1647 1572 527 516 435 378 259
152 118 114 14
ApaI G_GGCC'C
Cuts at: 2906 4189 2906
Size: 1283 7259
ApaLI G'TGCA_C
Cuts at: 4529 6599 7755 4529
Size: 2070 1156 5316
Fragments arranged by size:
5316 2070 1156
ApoI r'AATT_y
Cuts at: 1493 4904 5851 6077 6229 8338 1493
Size: 3411 947 226 152 2109 1697
Fragments arranged by size:
3411 2109 1697 947 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7096 160
Size: 872 6064 1606
Fragments arranged by size:
6064 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8542
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2360 2405 2423 2525 2683 3457
Size: 73 349 68 45 18 102 158 774
Cuts at: 3457 5429 5867 1870
Size: 1972 438 4545
Fragments arranged by size:
4545 1972 774 438 349 158 102 73
68 45 18
BamHI G'GATC_C
Cuts at: 2502 3916 5872 6239 2502
Size: 1414 1956 367 4805
Fragments arranged by size:
4805 1956 1414 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 2279 3195 3281 3366
Size: 325 217 627 364 128 916 86 85
Cuts at: 3366 4357 5221 5863 7318 618
Size: 991 864 642 1455 1842
Fragments arranged by size:
1842 1455 991 916 864 642 627 364
325 217 128 86 85
BanII G_rGCy'C
Cuts at: 1391 2082 2292 2361 2684 2906 3466 3681 3714
Size: 691 210 69 323 222 560 215 33
Cuts at: 3714 4189 4749 4928 5436 5861 1391
Size: 475 560 179 508 425 4072
Fragments arranged by size:
4072 691 560 560 508 475 425 323
222 215 210 179 69 33
BbeI G_GCGC'C
Cuts at: 2155 2283 3285 2155
Size: 128 1002 7412
Fragments arranged by size:
7412 1002 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4198 5167 928
Size: 971 2299 969 4303
Fragments arranged by size:
4303 2299 971 969
BbvCI CC'TCA_GC
Cuts at: 3419 3419
Size: 8542
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2418 2452 2854 2888 3288 3322 5062
Size: 34 481 34 402 34 400 34 1740
Cuts at: 5062 5096 6733 6767 1903
Size: 34 1637 34 3678
Fragments arranged by size:
3678 1740 1637 481 402 400 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4935 6434 7644 3320
Size: 1615 1499 1210 4218
Fragments arranged by size:
4218 1615 1499 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8542
BfrBI ATG'CAT
Cuts at: 2699 2699
Size: 8542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 2833 4542
Size: 107 122 71 631 605 802 359 1709
Cuts at: 4542 7153 136
Size: 2611 1525
Fragments arranged by size:
2611 1709 1525 802 631 605 359 122
107 71
BglII A'GATC_T
Cuts at: 3258 4392 8537 3258
Size: 1134 4145 3263
Fragments arranged by size:
4145 3263 1134
BlpI GC'TnA_GC
Cuts at: 4884 5959 4884
Size: 1075 7467
Bme1580I G_kGCm'C
Cuts at: 1790 2906 4189 4344 4362 4533 5226 6603 7759
Size: 1116 1283 155 18 171 693 1377 1156
Cuts at: 7759 1790
Size: 2573
Fragments arranged by size:
2573 1377 1283 1156 1116 693 171 155
18
BmgBI CAC'GTC
Cuts at: 1318 4204 5383 1318
Size: 2886 1179 4477
Fragments arranged by size:
4477 2886 1179
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6273 7231 442
Size: 416 53 83 399 24 4856 958 1753
Fragments arranged by size:
4856 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3549 1056
Size: 2493 6049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 3073 2116
Size: 957 7585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2177 2864 3025 3488 3593 4352 4673
Size: 908 30 687 161 463 105 759 321
Cuts at: 4673 4841 4940 5798 7202 1239
Size: 168 99 858 1404 2579
Fragments arranged by size:
2579 1404 908 858 759 687 463 321
168 161 105 99 30
Bpu10I CC'TnA_GC
Cuts at: 2483 3419 3563 3675 2483
Size: 936 144 112 7350
Fragments arranged by size:
7350 936 144 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4226 6667 7532 7830 4226
Size: 2441 865 298 4938
Fragments arranged by size:
4938 2441 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3460 3647 7205 882
Size: 617 1961 187 3558 2219
Fragments arranged by size:
3558 2219 1961 617 187
BsaAI yAC'GTr
Cuts at: 493 1204 2554 5839 6333 493
Size: 711 1350 3285 494 2702
Fragments arranged by size:
3285 2702 1350 711 494
BsaBI GATnn'nnATC
Cuts at: 4391 8536 4391
Size: 4145 4397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2152 2280 2535 2745
Size: 53 83 186 936 619 128 255 210
Cuts at: 2745 2766 2808 3240 3282 4050 5340 6349 6731
Size: 21 42 432 42 768 1290 1009 382
Cuts at: 6731 7366 275
Size: 635 1451
Fragments arranged by size:
1451 1290 1009 936 768 635 619 432
382 255 210 186 128 83 53 42
42 21
BsaWI w'CCGG_w
Cuts at: 5297 6975 7373 7647 7794 8205 5297
Size: 1678 398 274 147 411 5634
Fragments arranged by size:
5634 1678 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 3005 3023 3035
Size: 30 165 30 864 30 708 18 12
Cuts at: 3035 3053 3386 3416 4472 4502 5726 5756 1178
Size: 18 333 30 1056 30 1224 30 3964
Fragments arranged by size:
3964 1224 1056 864 708 333 165 30
30 30 30 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2716 3049 3062 3368 3394 3566 3991
Size: 1183 347 333 13 306 26 172 425
Cuts at: 3991 4106 4382 4558 5264 5288 1186
Size: 115 276 176 706 24 4440
Fragments arranged by size:
4440 1183 706 425 347 333 306 276
176 172 115 26 24 13
BseYI C'CCAG_C
Cuts at: 2667 3784 4511 5696 7745 8241 2667
Size: 1117 727 1185 2049 496 2968
Fragments arranged by size:
2968 2049 1185 1117 727 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3746 4216 4605 4798 5606 1163
Size: 573 2010 470 389 193 808 4099
Fragments arranged by size:
4099 2010 808 573 470 389 193
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 5992 6753 7781 1540
Size: 275 186 454 3537 761 1028 2301
Fragments arranged by size:
3537 2301 1028 761 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 2927 3681 3983 4140 4533 5003 6603 6688 7759
Size: 754 302 157 393 470 1600 85 1071
Cuts at: 7759 2927
Size: 3710
Fragments arranged by size:
3710 1600 1071 754 470 393 302 157
85
BsmI GAATG_Cn'
Cuts at: 4704 6053 6146 4704
Size: 1349 93 7100
Fragments arranged by size:
7100 1349 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3288 3288
Size: 8542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2292 2361 2684 2906 2927 3466
Size: 399 292 210 69 323 222 21 539
Cuts at: 3466 3681 3714 3983 4140 4189 4344 4362 4533
Size: 215 33 269 157 49 155 18 171
Cuts at: 4533 4749 4928 5003 5226 5436 5861 6603 6688
Size: 216 179 75 223 210 425 742 85
Cuts at: 6688 7759 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 539 425 399 323 292
269 223 222 216 215 210 210 179
171 157 155 85 75 69 49 33
21 18
BspEI T'CCGG_A
Cuts at: 8205 8205
Size: 8542
BspHI T'CATG_A
Cuts at: 6431 8161 6431
Size: 1730 6812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 2952 4230 5529 5556 5897 844
Size: 1361 747 1278 1299 27 341 3489
Fragments arranged by size:
3489 1361 1299 1278 747 341 27
BsrBI CCG'CTC
Cuts at: 1394 1823 2500 2908 3126 4254 4297 6429 1394
Size: 429 677 408 218 1128 43 2132 3507
Fragments arranged by size:
3507 2132 1128 677 429 408 218 43
BsrDI GCAATG_nn'
Cuts at: 65 1248 7037 7211 65
Size: 1183 5789 174 1396
Fragments arranged by size:
5789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 3198 3363 3628 4140 7186 7373
Size: 110 425 963 165 265 512 3046 187
Cuts at: 7373 1700
Size: 2869
Fragments arranged by size:
3046 2869 963 512 425 265 187 165
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8542
BssHII G'CGCG_C
Cuts at: 1925 2257 1925
Size: 332 8210
BssSI C'ACGA_G
Cuts at: 2573 4675 6602 7614 2573
Size: 2102 1927 1012 3501
Fragments arranged by size:
3501 2102 1927 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 5050 794
Size: 1412 2844 4286
Fragments arranged by size:
4286 2844 1412
BstBI TT'CG_AA
Cuts at: 2007 7346 2007
Size: 5339 3203
BstEII G'GTnAC_C
Cuts at: 1184 5197 1184
Size: 4013 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 2650 3235 3784 3832 2650
Size: 585 549 48 7360
Fragments arranged by size:
7360 585 549 48
BstYI r'GATC_y
Cuts at: 1972 2502 3258 3687 3916 4303 4392 4977 5872
Size: 530 756 429 229 387 89 585 895
Cuts at: 5872 6239 6624 6641 8093 8179 8191 8537 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 895 756 585 530 429 387
385 367 346 229 89 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4937 4937
Size: 8542
BtgI C'CryG_G
Cuts at: 513 1065 2022 2515 3289 3825 4650 5307 5634
Size: 552 957 493 774 536 825 657 327
Cuts at: 5634 513
Size: 3421
Fragments arranged by size:
3421 957 825 774 657 552 536 493
327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2257 3393 5065 5377 5461 487
Size: 683 56 1031 1136 1672 312 84 3568
Fragments arranged by size:
3568 1672 1136 1031 683 312 84 56
BtsI GCAGTG_nn'
Cuts at: 3188 3202 3578 3737 4344 4490 6058 6852 6872
Size: 14 376 159 607 146 1568 794 20
Cuts at: 6872 3188
Size: 4858
Fragments arranged by size:
4858 1568 794 607 376 159 146 20
14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5623 5658 529
Size: 35 5059 35 3413
Fragments arranged by size:
5059 3413 35 35
DraI TTT'AAA
Cuts at: 5846 5846
Size: 8542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7549 809
Size: 507 6233 1802
Fragments arranged by size:
6233 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2518 2834
Size: 54 1425 50 275 18 381 307 316
Cuts at: 2834 3064 3774 5304 5989 6878 8507 8
Size: 230 710 1530 685 889 1629 43
Fragments arranged by size:
1629 1530 1425 889 710 685 381 316
307 275 230 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5989 1537
Size: 275 4177 4090
Fragments arranged by size:
4177 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3719 3767 4421 5138 5444 6472 1353
Size: 866 1500 48 654 717 306 1028 3423
Fragments arranged by size:
3423 1500 1028 866 717 654 306 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2546 7129 7503 7649 387
Size: 1064 138 957 4583 374 146 1280
Fragments arranged by size:
4583 1280 1064 957 374 146 138
EcoICRI GAG'CTC
Cuts at: 3679 3679
Size: 8542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2177 2810 2864 3025 3488 3593
Size: 393 515 30 633 54 161 463 105
Cuts at: 3593 4025 4172 4352 4484 4507 4673 4841 4940
Size: 432 147 180 132 23 166 168 99
Cuts at: 4940 5345 5426 5798 6605 7202 7989 8257 1239
Size: 405 81 372 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 633 597 515 463 432
405 393 372 268 180 168 166 161
147 132 105 99 81 54 30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2588 5537 2159
Size: 429 2949 5164
Fragments arranged by size:
5164 2949 429
EcoO109I rG'GnC_Cy
Cuts at: 2269 2459 2706 2826 2902 2964 3526 4185 5047
Size: 190 247 120 76 62 562 659 862
Cuts at: 5047 2269
Size: 5764
Fragments arranged by size:
5764 862 659 562 247 190 120 76
62
EcoRI G'AATT_C
Cuts at: 5851 6229 5851
Size: 378 8164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4177 5350 5431 4177
Size: 1173 81 7288
Fragments arranged by size:
7288 1173 81
FspI TGC'GCA
Cuts at: 2915 7048 2915
Size: 4133 4409
HaeII r_GCGC'y
Cuts at: 1678 2134 2155 2182 2283 2324 2483 2947 3285
Size: 456 21 27 101 41 159 464 338
Cuts at: 3285 3364 5263 7689 1678
Size: 79 1899 2426 2531
Fragments arranged by size:
2531 2426 1899 464 456 338 159 101
79 41 27 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 3005 3037 3044
Size: 32 135 32 695 32 706 32 7
Cuts at: 3044 3076 3386 3418 4472 4504 4721 4753 4789
Size: 32 310 32 1054 32 217 32 36
Cuts at: 4789 4821 5711 5726 5743 5758 5799 5831 7183
Size: 32 890 15 17 15 41 32 1352
Cuts at: 7183 7215 7257 7289 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1352 1054 890 706 695 310 217
135 42 41 36 32 32 32 32
32 32 32 32 32 32 32 32
17 15 15 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 2749 5886 6132 669
Size: 988 281 658 153 3137 246 3079
Fragments arranged by size:
3137 3079 988 658 281 246 153
HindIII A'AGCT_T
Cuts at: 748 4633 748
Size: 3885 4657
HpaI GTT'AAC
Cuts at: 6132 6132
Size: 8542
KasI G'GCGC_C
Cuts at: 2151 2279 3281 2151
Size: 128 1002 7412
Fragments arranged by size:
7412 1002 128
KpnI G_GTAC'C
Cuts at: 5867 5867
Size: 8542
MfeI C'AATT_G
Cuts at: 6141 6141
Size: 8542
MluI A'CGCG_T
Cuts at: 8418 8418
Size: 8542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 2592 3944 4458 7656 7840 1305
Size: 1209 78 1352 514 3198 184 2007
Fragments arranged by size:
3198 2007 1352 1209 514 184 78
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 2520 3066 3776 5306
Size: 54 1425 343 381 307 546 710 1530
Cuts at: 5306 10
Size: 3246
Fragments arranged by size:
3246 1530 1425 710 546 381 343 307
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 3233 3372 3830 4121 5084 6500
Size: 648 1482 585 139 458 291 963 1416
Cuts at: 6500 6859 7018 518
Size: 359 159 2042
Fragments arranged by size:
2042 1482 1416 963 648 585 458 359
291 159 139
MspA1I CmG'CkG
Cuts at: 1606 2739 3611 3748 3784 4147 4711 4883 5035
Size: 1133 872 137 36 363 564 172 152
Cuts at: 5035 5724 5776 5958 6635 7783 8028 1606
Size: 689 52 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 872 689 677 564 363
245 182 172 152 137 52 36
NaeI GCC'GGC
Cuts at: 3365 3365
Size: 8542
NarI GG'CG_CC
Cuts at: 2152 2280 3282 2152
Size: 128 1002 7412
Fragments arranged by size:
7412 1002 128
NcoI C'CATG_G
Cuts at: 513 1065 2022 2515 3289 3825 513
Size: 552 957 493 774 536 5230
Fragments arranged by size:
5230 957 774 552 536 493
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8542
NgoMIV G'CCGG_C
Cuts at: 3363 3363
Size: 8542
NheI G'CTAG_C
Cuts at: 1052 3545 1052
Size: 2493 6049
NotI GC'GGCC_GC
Cuts at: 5989 5989
Size: 8542
NsiI A_TGCA'T
Cuts at: 2701 2701
Size: 8542
NspI r_CATG'y
Cuts at: 1132 1229 3109 5515 5900 7445 1132
Size: 97 1880 2406 385 1545 2229
Fragments arranged by size:
2406 2229 1880 1545 385 97
PasI CC'CwG_GG
Cuts at: 1348 2629 1348
Size: 1281 7261
PciI A'CATG_T
Cuts at: 7441 7441
Size: 8542
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3772 4321 5245 1108
Size: 2664 549 924 4405
Fragments arranged by size:
4405 2664 924 549
PfoI T'CCnGG_A
Cuts at: 2067 3111 4605 2067
Size: 1044 1494 6004
Fragments arranged by size:
6004 1494 1044
PmeI GTTT'AAAC
Cuts at: 5846 5846
Size: 8542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 6611 6643 8124 8156 3023
Size: 32 3556 32 1481 32 3409
Fragments arranged by size:
3556 3409 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2459 2964 3526 2459
Size: 505 562 7475
Fragments arranged by size:
7475 562 505
PshAI GACnn'nnGTC
Cuts at: 1613 4111 4865 5171 1613
Size: 2498 754 306 4984
Fragments arranged by size:
4984 2498 754 306
PsiI TTA'TAA
Cuts at: 6112 6112
Size: 8542
PspOMI G'GGCC_C
Cuts at: 2902 4185 2902
Size: 1283 7259
PspXI vC'TCGA_Gb
Cuts at: 2683 2683
Size: 8542
PstI C_TGCA'G
Cuts at: 830 2202 3020 3343 4047 4662 5055 5775 5894
Size: 1372 818 323 704 615 393 720 119
Cuts at: 5894 830
Size: 3478
Fragments arranged by size:
3478 1372 818 720 704 615 393 323
119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8542
PvuII CAG'CTG
Cuts at: 2739 3784 4147 4883 5035 5724 5776 2739
Size: 1045 363 736 152 689 52 5505
Fragments arranged by size:
5505 1045 736 689 363 152 52
RsrII CG'GwC_CG
Cuts at: 2614 2614
Size: 8542
SacI G_AGCT'C
Cuts at: 3681 3681
Size: 8542
SalI G'TCGA_C
Cuts at: 1936 2747 5884 1936
Size: 811 3137 4594
Fragments arranged by size:
4594 3137 811
SanDI GG'GwC_CC
Cuts at: 2459 2459
Size: 8542
SapI GCTCTTCn'nnn_
Cuts at: 2219 3719 2219
Size: 1500 7042
SbfI CC_TGCA'GG
Cuts at: 5055 5894 5055
Size: 839 7703
ScaI AGT'ACT
Cuts at: 6790 8502 6790
Size: 1712 6830
SfcI C'TryA_G
Cuts at: 826 1045 2198 3016 3339 4043 4658 4870 5051
Size: 219 1153 818 323 704 615 212 181
Cuts at: 5051 5771 5890 7025 7706 7897 826
Size: 720 119 1135 681 191 1471
Fragments arranged by size:
1471 1153 1135 818 720 704 681 615
323 219 212 191 181 119
SfiI GGCCn_nnn'nGGCC
Cuts at: 2833 2833
Size: 8542
SfoI GGC'GCC
Cuts at: 2153 2281 3283 2153
Size: 128 1002 7412
Fragments arranged by size:
7412 1002 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8542
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8542
SmaI CCC'GGG
Cuts at: 1872 2407 2425 2527 5869 1872
Size: 535 18 102 3342 4545
Fragments arranged by size:
4545 3342 535 102 18
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2683 4241 6646 7547 7809 820
Size: 197 926 740 1558 2405 901 262 1553
Fragments arranged by size:
2405 1558 1553 926 901 740 262 197
SnaBI TAC'GTA
Cuts at: 493 5839 6333 493
Size: 5346 494 2702
Fragments arranged by size:
5346 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8542
SphI G_CATG'C
Cuts at: 1132 5515 5900 1132
Size: 4383 385 3774
Fragments arranged by size:
4383 3774 385
SspI AAT'ATT
Cuts at: 5 52 6466 5
Size: 47 6414 2081
Fragments arranged by size:
6414 2081 47
StuI AGG'CCT
Cuts at: 1517 2141 1517
Size: 624 7918
StyI C'CwwG_G
Cuts at: 513 1065 2022 2214 2515 2831 3289 3825 3912
Size: 552 957 192 301 316 458 536 87
Cuts at: 3912 4983 5217 5730 5981 513
Size: 1071 234 513 251 3074
Fragments arranged by size:
3074 1071 957 552 536 513 458 316
301 251 234 192 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 5101 6569 6586 6739 6924 7369
Size: 1255 2196 1325 1468 17 153 185 445
Cuts at: 7369 325
Size: 1498
Fragments arranged by size:
2196 1498 1468 1325 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4809 5412 5763
Size: 274 80 33 51 1443 2832 603 351
Cuts at: 5763 6788 8500 96
Size: 1025 1712 138
Fragments arranged by size:
2832 1712 1443 1025 603 351 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4056 4088 8502 8534 4056
Size: 32 4414 32 4064
Fragments arranged by size:
4414 4064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8542
XbaI T'CTAG_A
Cuts at: 5878 5878
Size: 8542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 3934 4351 4781 2152
Size: 1782 417 430 5913
Fragments arranged by size:
5913 1782 430 417
XhoI C'TCGA_G
Cuts at: 1943 2683 1943
Size: 740 7802
XmaI C'CCGG_G
Cuts at: 1870 2405 2423 2525 5867 1870
Size: 535 18 102 3342 4545
Fragments arranged by size:
4545 3342 535 102 18
XmnI GAAnn'nnTTC
Cuts at: 2546 4625 6671 2546
Size: 2079 2046 4417
Fragments arranged by size:
4417 2079 2046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5341 6350 276
Size: 53 83 186 936 3807 1009 2468
Fragments arranged by size:
3807 2468 1009 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoRV
FseI FspAI NruI PacI PmlI PsrI SacII SexAI
SrfI SwaI