Restriction map for
pFN21ASDB0003
(Circular) (Six-base) MAPSORT of: pFN21ASDB0003.seq Check: 2981 from: 1 to: 6235
pFN21ASDB0003 6235 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2472 2472
Size: 6235
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2721 4045 278
Size: 53 83 186 936 1185 1324 2468
Fragments arranged by size:
2468 1324 1185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3295 3578 1937
Size: 1358 283 4594
Fragments arranged by size:
4594 1358 283
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3107 4298 5682 5950 1632
Size: 1475 1191 1384 268 1917
Fragments arranged by size:
1917 1475 1384 1191 268
AflII C'TTAA_G
Cuts at: 820 1017 3085 820
Size: 197 2068 3970
Fragments arranged by size:
3970 2068 197
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 2581 1166
Size: 1415 4820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 3001 3978 5550 3001
Size: 977 1572 3686
Fragments arranged by size:
3686 1572 977
ApaI G_GGCC'C
Cuts at: 3349 3349
Size: 6235
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 3044 3209 3239 3544 3770 3922 6031 1493
Size: 1551 165 30 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1551 305 226 165 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 3560 1870
Size: 73 161 1456 4545
Fragments arranged by size:
4545 1456 161 73
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2369 3556 5011 618
Size: 325 217 627 334 248 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 334 325 248 217
BanII G_rGCy'C
Cuts at: 1391 3008 3349 3554 1391
Size: 1617 341 205 4072
Fragments arranged by size:
4072 1617 341 205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 928
Size: 971 582 4682
Fragments arranged by size:
4682 971 582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 5623
BfrBI ATG'CAT
Cuts at: 2420 2420
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6230 6230
Size: 6235
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 3349 4296 5452 1790
Size: 1559 947 1156 2573
Fragments arranged by size:
2573 1559 1156 947
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3406 3966 4924
Size: 416 53 83 399 24 1989 560 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1989 1753 958 560 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2924 3113 4895 1239
Size: 1685 189 1782 2579
Fragments arranged by size:
2579 1782 1685 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4360 5225 5523 4360
Size: 865 298 5072
Fragments arranged by size:
5072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4898 882
Size: 617 3399 2219
Fragments arranged by size:
3399 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3532 4026 493
Size: 711 2328 494 2702
Fragments arranged by size:
2702 2328 711 494
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2718 4042 4424 5059
Size: 53 83 186 936 1185 1324 382 635
Cuts at: 5059 275
Size: 1451
Fragments arranged by size:
1451 1324 1185 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4668 5066 5340 5487 5898 4668
Size: 398 274 147 411 5005
Fragments arranged by size:
5005 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6010
Fragments arranged by size:
6010 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 3521 1186
Size: 903 1432 3900
Fragments arranged by size:
3900 1432 903
BseYI C'CCAG_C
Cuts at: 2050 3001 3385 5438 5934 2050
Size: 951 384 2053 496 2351
Fragments arranged by size:
2351 2053 951 496 384
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 1163
Size: 573 708 4954
Fragments arranged by size:
4954 708 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2117 3685 4446 5474 1540
Size: 275 186 116 1568 761 1028 2301
Fragments arranged by size:
2301 1568 1028 761 275 186 116
BsiHKAI G_wGCw'C
Cuts at: 4296 4381 5452 4296
Size: 85 1071 5079
Fragments arranged by size:
5079 1071 85
BsmI GAATG_Cn'
Cuts at: 2223 3746 3839 2223
Size: 1523 93 4619
Fragments arranged by size:
4619 1523 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3008 3349 3554 4296 4381 5452 1391
Size: 399 1218 341 205 742 85 1071 2174
Fragments arranged by size:
2174 1218 1071 742 399 341 205 85
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 3309 4124 5854 3309
Size: 815 1730 3690
Fragments arranged by size:
3690 1730 815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2472 3590 844
Size: 1628 1118 3489
Fragments arranged by size:
3489 1628 1118
BsrBI CCG'CTC
Cuts at: 1394 1823 3331 4122 1394
Size: 429 1508 791 3507
Fragments arranged by size:
3507 1508 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4730 4904 65
Size: 1183 3482 174 1396
Fragments arranged by size:
3482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2112 4879 5066 1700
Size: 110 302 2767 187 2869
Fragments arranged by size:
2869 2767 302 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3118 794
Size: 2324 3911
BstBI TT'CG_AA
Cuts at: 2007 5039 2007
Size: 3032 3203
BstEII G'GTnAC_C
Cuts at: 1184 2635 1184
Size: 1451 4784
BstYI r'GATC_y
Cuts at: 1972 3565 3932 4317 4334 5786 5872 5884 6230
Size: 1593 367 385 17 1452 86 12 346
Cuts at: 6230 1972
Size: 1977
Fragments arranged by size:
1977 1593 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3296 3296
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 2305 2602 513
Size: 552 1240 297 4146
Fragments arranged by size:
4146 1240 552 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 2734 3191 3463 3751 4545 4565 2734
Size: 457 272 288 794 20 4404
Fragments arranged by size:
4404 794 457 288 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DraIII CAC_nnn'GTG
Cuts at: 2821 2821
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2277 5242 809
Size: 507 961 2965 1802
Fragments arranged by size:
2965 1802 961 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2114 3682 4571
Size: 54 1425 50 275 18 284 1568 889
Cuts at: 4571 6200 8
Size: 1629 43
Fragments arranged by size:
1629 1568 1425 889 284 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2114 3682 1537
Size: 275 302 1568 4090
Fragments arranged by size:
4090 1568 302 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2597 3338 3413 4165 1353
Size: 1244 741 75 752 3423
Fragments arranged by size:
3423 1244 752 741 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2321 4822 5196 5342 387
Size: 1064 138 732 2501 374 146 1280
Fragments arranged by size:
2501 1280 1064 732 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2924 3107 3113 4298 4895 5682 5950
Size: 393 1292 183 6 1185 597 787 268
Cuts at: 5950 1239
Size: 1524
Fragments arranged by size:
1524 1292 1185 787 597 393 268 183
6
EcoRI G'AATT_C
Cuts at: 3209 3544 3922 3209
Size: 335 378 5522
Fragments arranged by size:
5522 378 335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2498 3245 2498
Size: 747 5488
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 5382 1678
Size: 3704 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2640 2672 4876 4908 4950
Size: 32 135 32 1068 32 2204 32 42
Cuts at: 4950 4982 1373
Size: 32 2626
Fragments arranged by size:
2626 2204 1068 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3579 3825 669
Size: 988 281 1641 246 3079
Fragments arranged by size:
3079 1641 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2398 2756 748
Size: 1650 358 4227
Fragments arranged by size:
4227 1650 358
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 2928 3834 2928
Size: 906 5329
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3354 5349 5533 1305
Size: 2049 1995 184 2007
Fragments arranged by size:
2049 2007 1995 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2581 4193 4552 4711 518
Size: 648 861 554 1612 359 159 2042
Fragments arranged by size:
2042 1612 861 648 554 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 3001 3465 3651 4328 5476 5721 1606
Size: 531 864 464 186 677 1148 245 2120
Fragments arranged by size:
2120 1148 864 677 531 464 245 186
NaeI GCC'GGC
Cuts at: 2114 2114
Size: 6235
NcoI C'CATG_G
Cuts at: 513 1065 2602 513
Size: 552 1537 4146
Fragments arranged by size:
4146 1537 552
NdeI CA'TA_TG
Cuts at: 387 2499 387
Size: 2112 4123
NgoMIV G'CCGG_C
Cuts at: 2112 2112
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NsiI A_TGCA'T
Cuts at: 2422 2422
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 2938 3385 3593 5138 1132
Size: 97 1709 447 208 1545 2229
Fragments arranged by size:
2229 1709 1545 447 208 97
PasI CC'CwG_GG
Cuts at: 1348 2142 2831 1348
Size: 794 689 4752
Fragments arranged by size:
4752 794 689
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6235
PfoI T'CCnGG_A
Cuts at: 2257 3336 2257
Size: 1079 5156
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 3345 3345
Size: 6235
PstI C_TGCA'G
Cuts at: 830 2205 2809 3587 830
Size: 1375 604 778 3478
Fragments arranged by size:
3478 1375 778 604
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 3001 3001
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SapI GCTCTTCn'nnn_
Cuts at: 3338 3413 3338
Size: 75 6160
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SfcI C'TryA_G
Cuts at: 826 1045 2201 2805 3214 3583 4718 5399 5590
Size: 219 1156 604 409 369 1135 681 191
Cuts at: 5590 826
Size: 1471
Fragments arranged by size:
1471 1156 1135 681 604 409 369 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 2106 3562 1872
Size: 234 1456 4545
Fragments arranged by size:
4545 1456 234
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3085 4339 5240 5502 820
Size: 197 926 1142 1254 901 262 1553
Fragments arranged by size:
1553 1254 1142 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3532 4026 493
Size: 3039 494 2702
Fragments arranged by size:
3039 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 2938 3593 1132
Size: 1806 655 3774
Fragments arranged by size:
3774 1806 655
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2482 2602 3490 3674 513
Size: 552 1417 120 888 184 3074
Fragments arranged by size:
3074 1417 888 552 184 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 2738 2844 4262 4279 4432 4617
Size: 1255 484 674 106 1418 17 153 185
Cuts at: 4617 5062 325
Size: 445 1498
Fragments arranged by size:
1498 1418 1255 674 484 445 185 153
106 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2345 4481 6193
Size: 274 80 33 51 1443 368 2136 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
2136 1712 1443 368 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6195 6227 6195
Size: 32 6203
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 2104 3560 1870
Size: 234 1456 4545
Fragments arranged by size:
4545 1456 234
XmnI GAAnn'nnTTC
Cuts at: 4364 4364
Size: 6235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2719 4043 276
Size: 53 83 186 936 1185 1324 2468
Fragments arranged by size:
2468 1324 1185 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI