Restriction map for
pFN21ASDB0002
(Circular) (Six-base) MAPSORT of: pFN21ASDB0002.seq Check: 598 from: 1 to: 8455
pFN21ASDB0002 8455 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2728 2728
Size: 8455
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6265 278
Size: 53 83 186 936 4729 2468
Fragments arranged by size:
4729 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5798 1937
Size: 3861 4594
Acc65I G'GTAC_C
Cuts at: 2340 5776 2340
Size: 3436 5019
AclI AA'CG_TT
Cuts at: 1563 6582 6955 1563
Size: 5019 373 3063
Fragments arranged by size:
5019 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 6518 7902 8170 1632
Size: 914 3972 1384 268 1917
Fragments arranged by size:
3972 1917 1384 914 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8258
AflIII A'CryG_T
Cuts at: 1203 4224 7354 8331 1203
Size: 3021 3130 977 1327
Fragments arranged by size:
3130 3021 1327 977
AgeI A'CCGG_T
Cuts at: 7286 7286
Size: 8455
AhdI GACnn_n'nnGTC
Cuts at: 7184 7184
Size: 8455
AleI CACnn'nnGTG
Cuts at: 1166 2367 5243 5302 5357 1166
Size: 1201 2876 59 55 4264
Fragments arranged by size:
4264 2876 1201 59 55
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2385 5387 1184
Size: 1201 3002 4252
Fragments arranged by size:
4252 3002 1201
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 8423
AlwNI CAG_nnn'CTG
Cuts at: 2661 3327 4531 5144 6198 7770 2661
Size: 666 1204 613 1054 1572 3346
Fragments arranged by size:
3346 1572 1204 1054 666 613
ApaI G_GGCC'C
Cuts at: 2144 2721 3441 4645 4922 5210 2144
Size: 577 720 1204 277 288 5389
Fragments arranged by size:
5389 1204 720 577 288 277
ApaLI G'TGCA_C
Cuts at: 5605 6512 7668 5605
Size: 907 1156 6392
Fragments arranged by size:
6392 1156 907
ApoI r'AATT_y
Cuts at: 1493 4721 5764 5990 6142 8251 1493
Size: 3228 1043 226 152 2109 1697
Fragments arranged by size:
3228 2109 1697 1043 226 152
AscI GG'CGCG_CC
Cuts at: 2147 2147
Size: 8455
AseI AT'TA_AT
Cuts at: 160 1032 7009 160
Size: 872 5977 1606
Fragments arranged by size:
5977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8455
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 2286 2402 2720 3480 4352 4638
Size: 73 210 133 116 318 760 872 286
Cuts at: 4638 4644 4699 4931 4959 5241 5355 5780 1870
Size: 6 55 232 28 282 114 425 4545
Fragments arranged by size:
4545 872 760 425 318 286 282 232
210 133 116 114 73 55 28 6
BamHI G'GATC_C
Cuts at: 4615 5785 6152 4615
Size: 1170 367 6918
Fragments arranged by size:
6918 1170 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2340 2880 3023 3813 4370
Size: 325 217 627 553 540 143 790 557
Cuts at: 4370 5304 5776 7231 618
Size: 934 472 1455 1842
Fragments arranged by size:
1842 1455 934 790 627 557 553 540
472 325 217 143
BanII G_rGCy'C
Cuts at: 1391 2033 2144 2721 3441 3932 4503 4645 4692
Size: 642 111 577 720 491 571 142 47
Cuts at: 4692 4700 4922 5210 5500 5774 1391
Size: 8 222 288 290 274 4072
Fragments arranged by size:
4072 720 642 577 571 491 290 288
274 222 142 111 47 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3641 928
Size: 971 1742 5742
Fragments arranged by size:
5742 1742 971
BbvCI CC'TCA_GC
Cuts at: 5134 5474 5134
Size: 340 8115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2282 2316 2375 2409 4882 4916 6646
Size: 34 345 34 59 34 2473 34 1730
Cuts at: 6646 6680 1903
Size: 34 3678
Fragments arranged by size:
3678 2473 1730 345 59 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 6347 7557 4472
Size: 1875 1210 5370
Fragments arranged by size:
5370 1875 1210
BclI T'GATC_A
Cuts at: 1548 4732 1548
Size: 3184 5271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2132 2309 2405
Size: 107 122 71 631 605 460 177 96
Cuts at: 2405 2795 3903 4600 5114 7066 136
Size: 390 1108 697 514 1952 1525
Fragments arranged by size:
1952 1525 1108 697 631 605 514 460
390 177 122 107 96 71
BglII A'GATC_T
Cuts at: 3568 8450 3568
Size: 4882 3573
BlpI GC'TnA_GC
Cuts at: 3407 5872 3407
Size: 2465 5990
Bme1580I G_kGCm'C
Cuts at: 1790 2144 2487 2721 3441 3527 3756 3816 3891
Size: 354 343 234 720 86 229 60 75
Cuts at: 3891 4375 4645 4922 4971 5210 5376 5597 5609
Size: 484 270 277 49 239 166 221 12
Cuts at: 5609 6516 7672 1790
Size: 907 1156 2573
Fragments arranged by size:
2573 1156 907 720 484 354 343 277
270 239 234 229 221 166 86 75
60 49 12
BmgBI CAC'GTC
Cuts at: 1318 2059 2482 1318
Size: 741 423 7291
Fragments arranged by size:
7291 741 423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3640 3878 3885
Size: 416 53 83 399 24 2223 238 7
Cuts at: 3885 4166 4905 4925 5166 5340 6186 7144 442
Size: 281 739 20 241 174 846 958 1753
Fragments arranged by size:
2223 1753 958 846 739 416 399 281
241 238 174 83 53 24 20 7
BmtI G_CTAG'C
Cuts at: 1056 4944 1056
Size: 3888 4567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 5350 5689 3334
Size: 2016 339 6100
Fragments arranged by size:
6100 2016 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2804 2992 3304 4517 4668 4787 7115 1239
Size: 1565 188 312 1213 151 119 2328 2579
Fragments arranged by size:
2579 2328 1565 1213 312 188 151 119
Bpu10I CC'TnA_GC
Cuts at: 2373 5134 5474 2373
Size: 2761 340 5354
Fragments arranged by size:
5354 2761 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2488 5148 5671 6580 7445 7743 2240
Size: 248 2660 523 909 865 298 2952
Fragments arranged by size:
2952 2660 909 865 523 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 7118 882
Size: 617 1269 4350 2219
Fragments arranged by size:
4350 2219 1269 617
BsaAI yAC'GTr
Cuts at: 493 1204 5026 5752 6246 493
Size: 711 3822 726 494 2702
Fragments arranged by size:
3822 2702 726 711 494
BsaBI GATnn'nnATC
Cuts at: 2564 2651 8449 2564
Size: 87 5798 2570
Fragments arranged by size:
5798 2570 87
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 6262 6644 7279
Size: 53 83 186 936 2443 2286 382 635
Cuts at: 7279 275
Size: 1451
Fragments arranged by size:
2443 2286 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2035 3336 3361 6888 7286 7560 7707 8118 2035
Size: 1301 25 3527 398 274 147 411 2372
Fragments arranged by size:
3527 2372 1301 411 398 274 147 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3125 3155 3725 3755 1178
Size: 30 165 30 1722 30 570 30 5878
Fragments arranged by size:
5878 1722 570 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3292 3413 3416 3683 3788 4379 4853 5102
Size: 2106 121 3 267 105 591 474 249
Cuts at: 5102 5116 5326 1186
Size: 14 210 4315
Fragments arranged by size:
4315 2106 591 474 267 249 210 121
105 14 3
BseYI C'CCAG_C
Cuts at: 2135 2456 2903 3037 3068 3281 3542 4022 4058
Size: 321 447 134 31 213 261 480 36
Cuts at: 4058 4290 4344 4502 4797 4970 5200 5531 7658
Size: 232 54 158 295 173 230 331 2127
Cuts at: 7658 8154 2135
Size: 496 2436
Fragments arranged by size:
2436 2127 496 480 447 331 321 295
261 232 230 213 173 158 134 54
36 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 3101 3487 4661 4908 5310 1163
Size: 573 609 756 386 1174 247 402 4308
Fragments arranged by size:
4308 1174 756 609 573 402 386 247
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2631 5905 6666 7694 1540
Size: 275 186 630 3274 761 1028 2301
Fragments arranged by size:
3274 2301 1028 761 630 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 4895 5609 6516 6601 7672 2298
Size: 2597 714 907 85 1071 3081
Fragments arranged by size:
3081 2597 1071 907 714 85
BsmI GAATG_Cn'
Cuts at: 2498 5966 6059 2498
Size: 3468 93 4894
Fragments arranged by size:
4894 3468 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2144 2298 2487 2721 3441 3527
Size: 399 243 111 154 189 234 720 86
Cuts at: 3527 3756 3816 3891 3932 4375 4503 4645 4692
Size: 229 60 75 41 443 128 142 47
Cuts at: 4692 4700 4895 4922 4971 5210 5376 5500 5597
Size: 8 195 27 49 239 166 124 97
Cuts at: 5597 5609 5774 6516 6601 7672 1391
Size: 12 165 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 443 399 243 239
234 229 195 189 166 165 154 142
128 124 111 97 86 85 75 60
49 47 41 27 12 8
BspEI T'CCGG_A
Cuts at: 2035 3336 3361 8118 2035
Size: 1301 25 4757 2372
Fragments arranged by size:
4757 2372 1301 25
BspHI T'CATG_A
Cuts at: 6344 8074 6344
Size: 1730 6725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2728 5810 844
Size: 1241 643 3082 3489
Fragments arranged by size:
3489 3082 1241 643
BsrBI CCG'CTC
Cuts at: 1394 1823 3127 3141 3762 5384 5685 6342 1394
Size: 429 1304 14 621 1622 301 657 3507
Fragments arranged by size:
3507 1622 1304 657 621 429 301 14
BsrDI GCAATG_nn'
Cuts at: 65 1248 3496 4319 5076 6950 7124 65
Size: 1183 2248 823 757 1874 174 1396
Fragments arranged by size:
2248 1874 1396 1183 823 757 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2877 3093 3154 5307 7099 7286 1700
Size: 110 1067 216 61 2153 1792 187 2869
Fragments arranged by size:
2869 2153 1792 1067 216 187 110 61
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8455
BssHII G'CGCG_C
Cuts at: 1925 2119 2147 1925
Size: 194 28 8233
Fragments arranged by size:
8233 194 28
BssSI C'ACGA_G
Cuts at: 2771 3526 4597 6515 7527 2771
Size: 755 1071 1918 1012 3699
Fragments arranged by size:
3699 1918 1071 1012 755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3560 4672 5144 794
Size: 2766 1112 472 4105
Fragments arranged by size:
4105 2766 1112 472
BstBI TT'CG_AA
Cuts at: 2007 7259 2007
Size: 5252 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8455
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2911 2971 5359 2539
Size: 372 60 2388 5635
Fragments arranged by size:
5635 2388 372 60
BstYI r'GATC_y
Cuts at: 1972 3568 4328 4615 5785 6152 6537 6554 8006
Size: 1596 760 287 1170 367 385 17 1452
Cuts at: 8006 8092 8104 8450 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1596 1452 1170 760 385 367 346
287 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4281 5598 4281
Size: 1317 7138
BtgI C'CryG_G
Cuts at: 513 1065 2022 2631 3220 3248 3391 5112 513
Size: 552 957 609 589 28 143 1721 3856
Fragments arranged by size:
3856 1721 957 609 589 552 143 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2058 2290 3300 487
Size: 683 56 832 232 1010 5642
Fragments arranged by size:
5642 1010 832 683 232 56
BtsI GCAGTG_nn'
Cuts at: 3358 3718 3913 4971 5971 6765 6785 3358
Size: 360 195 1058 1000 794 20 5028
Fragments arranged by size:
5028 1058 1000 794 360 195 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8455
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5503 5538 529
Size: 35 4939 35 3446
Fragments arranged by size:
4939 3446 35 35
DraI TTT'AAA
Cuts at: 5759 5759
Size: 8455
DraIII CAC_nnn'GTG
Cuts at: 2272 5348 2272
Size: 3076 5379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7462 809
Size: 507 6146 1802
Fragments arranged by size:
6146 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2628 2665
Size: 54 1425 50 275 18 294 504 37
Cuts at: 2665 2961 3177 3217 3493 3895 5902 6791 8420
Size: 296 216 40 276 402 2007 889 1629
Cuts at: 8420 8
Size: 43
Fragments arranged by size:
2007 1629 1425 889 504 402 296 294
276 275 216 54 50 43 40 37
18
EagI C'GGCC_G
Cuts at: 1537 1812 2628 5902 1537
Size: 275 816 3274 4090
Fragments arranged by size:
4090 3274 816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3248 4453 4818 6385 1353
Size: 1895 1205 365 1567 3423
Fragments arranged by size:
3423 1895 1567 1205 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2327 2804 3733 4379 5326 7042
Size: 1064 138 738 477 929 646 947 1716
Cuts at: 7042 7416 7562 387
Size: 374 146 1280
Fragments arranged by size:
1716 1280 1064 947 929 738 646 477
374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2804 2992 3304 4517 4668 4787
Size: 393 914 258 188 312 1213 151 119
Cuts at: 4787 6518 7115 7902 8170 1239
Size: 1731 597 787 268 1524
Fragments arranged by size:
1731 1524 1213 914 787 597 393 312
268 258 188 151 119
EcoNI CCTnn'n_nnAGG
Cuts at: 2450 4206 2450
Size: 1756 6699
EcoO109I rG'GnC_Cy
Cuts at: 2140 2492 2536 2717 3437 3438 4285 4919 5206
Size: 352 44 181 720 1 847 634 287
Cuts at: 5206 5216 2140
Size: 10 5379
Fragments arranged by size:
5379 847 720 634 352 287 181 44
10 1
EcoRI G'AATT_C
Cuts at: 5764 6142 5764
Size: 378 8077
FspI TGC'GCA
Cuts at: 2063 2081 6961 2063
Size: 18 4880 3557
Fragments arranged by size:
4880 3557 18
HaeII r_GCGC'y
Cuts at: 1678 2199 2662 3111 4351 5235 5706 7602 1678
Size: 521 463 449 1240 884 471 1896 2531
Fragments arranged by size:
2531 1896 1240 884 521 471 463 449
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 3949 3981 4430
Size: 32 135 32 464 32 1881 32 449
Cuts at: 4430 4462 4993 5025 5146 5178 7096 7128 7170
Size: 32 531 32 121 32 1918 32 42
Cuts at: 7170 7202 1373
Size: 32 2626
Fragments arranged by size:
2626 1918 1881 531 464 449 135 121
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5799 6045 669
Size: 988 281 3861 246 3079
Fragments arranged by size:
3861 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8455
HpaI GTT'AAC
Cuts at: 6045 6045
Size: 8455
KpnI G_GTAC'C
Cuts at: 2344 5780 2344
Size: 3436 5019
MfeI C'AATT_G
Cuts at: 6054 6054
Size: 8455
MluI A'CGCG_T
Cuts at: 8331 8331
Size: 8455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 4269 4287 7569 7753 1305
Size: 1563 1401 18 3282 184 2007
Fragments arranged by size:
3282 2007 1563 1401 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2667 3219 3495 10
Size: 54 1425 343 835 552 276 4970
Fragments arranged by size:
4970 1425 835 552 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2693 3149 3680 3846 3863 4013
Size: 648 1201 326 456 531 166 17 150
Cuts at: 4013 4146 4223 4850 4994 5000 5243 5302 5357
Size: 133 77 627 144 6 243 59 55
Cuts at: 5357 6413 6772 6931 518
Size: 1056 359 159 2042
Fragments arranged by size:
2042 1201 1056 648 627 531 456 359
326 243 166 159 150 144 133 77
59 55 17 6
MspA1I CmG'CkG
Cuts at: 1606 2337 2359 2427 2433 2627 2644 3165 3393
Size: 731 22 68 6 194 17 521 228
Cuts at: 3393 3501 4026 4185 4565 5042 5661 5871 6548
Size: 108 525 159 380 477 619 210 677
Cuts at: 6548 7696 7941 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 731 677 619 525 521 477
380 245 228 210 194 159 108 68
22 17 6
NaeI GCC'GGC
Cuts at: 2879 3156 5309 2879
Size: 277 2153 6025
Fragments arranged by size:
6025 2153 277
NcoI C'CATG_G
Cuts at: 513 1065 2022 3220 5112 513
Size: 552 957 1198 1892 3856
Fragments arranged by size:
3856 1892 1198 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8455
NgoMIV G'CCGG_C
Cuts at: 2877 3154 5307 2877
Size: 277 2153 6025
Fragments arranged by size:
6025 2153 277
NheI G'CTAG_C
Cuts at: 1052 4940 1052
Size: 3888 4567
NotI GC'GGCC_GC
Cuts at: 5902 5902
Size: 8455
NspI r_CATG'y
Cuts at: 1132 1229 2098 2770 3862 4228 5068 5813 7358
Size: 97 869 672 1092 366 840 745 1545
Cuts at: 7358 1132
Size: 2229
Fragments arranged by size:
2229 1545 1092 869 840 745 672 366
97
PasI CC'CwG_GG
Cuts at: 1348 3370 3872 4609 5103 5653 1348
Size: 2022 502 737 494 550 4150
Fragments arranged by size:
4150 2022 737 550 502 494
PciI A'CATG_T
Cuts at: 4224 7354 4224
Size: 3130 5325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3707 3991 1108
Size: 2599 284 5572
Fragments arranged by size:
5572 2599 284
PfoI T'CCnGG_A
Cuts at: 2781 4094 2781
Size: 1313 7142
PmeI GTTT'AAAC
Cuts at: 5759 5759
Size: 8455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6524 6556 8037 8069 6524
Size: 32 1481 32 6910
Fragments arranged by size:
6910 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2492 4285 5216 2492
Size: 1793 931 5731
Fragments arranged by size:
5731 1793 931
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8455
PsiI TTA'TAA
Cuts at: 6025 6025
Size: 8455
PspOMI G'GGCC_C
Cuts at: 2140 2717 3437 4641 4918 5206 2140
Size: 577 720 1204 277 288 5389
Fragments arranged by size:
5389 1204 720 577 288 277
PspXI vC'TCGA_Gb
Cuts at: 2153 2153
Size: 8455
PstI C_TGCA'G
Cuts at: 830 2432 2643 3568 4536 5086 5807 830
Size: 1602 211 925 968 550 721 3478
Fragments arranged by size:
3478 1602 968 925 721 550 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8455
PvuII CAG'CTG
Cuts at: 2337 2359 2427 2644 4565 5042 2337
Size: 22 68 217 1921 477 5750
Fragments arranged by size:
5750 1921 477 217 68 22
SacII CC_GC'GG
Cuts at: 3394 3394
Size: 8455
SalI G'TCGA_C
Cuts at: 1936 5797 1936
Size: 3861 4594
SbfI CC_TGCA'GG
Cuts at: 5807 5807
Size: 8455
ScaI AGT'ACT
Cuts at: 2159 6703 8415 2159
Size: 4544 1712 2199
Fragments arranged by size:
4544 2199 1712
SexAI A'CCwGG_T
Cuts at: 2268 2268
Size: 8455
SfcI C'TryA_G
Cuts at: 826 1045 2354 2428 2639 3564 4532 5082 5803
Size: 219 1309 74 211 925 968 550 721
Cuts at: 5803 6938 7619 7810 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1309 1135 968 925 721 681 550
219 211 191 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 2795 2795
Size: 8455
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8455
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8455
SmaI CCC'GGG
Cuts at: 1872 2288 2722 4354 4701 4933 4961 5243 5357
Size: 416 434 1632 347 232 28 282 114
Cuts at: 5357 5782 1872
Size: 425 4545
Fragments arranged by size:
4545 1632 434 425 416 347 282 232
114 28
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2219 2467 5163 5686 6559
Size: 197 926 210 66 248 2696 523 873
Cuts at: 6559 7460 7722 820
Size: 901 262 1553
Fragments arranged by size:
2696 1553 926 901 873 523 262 248
210 197 66
SnaBI TAC'GTA
Cuts at: 493 5752 6246 493
Size: 5259 494 2702
Fragments arranged by size:
5259 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8455
SphI G_CATG'C
Cuts at: 1132 2098 5813 1132
Size: 966 3715 3774
Fragments arranged by size:
3774 3715 966
SspI AAT'ATT
Cuts at: 5 52 4717 6379 5
Size: 47 4665 1662 2081
Fragments arranged by size:
4665 2081 1662 47
StuI AGG'CCT
Cuts at: 1517 4954 5479 1517
Size: 3437 525 4493
Fragments arranged by size:
4493 3437 525
StyI C'CwwG_G
Cuts at: 513 1065 2022 2446 2532 3220 3537 3795 4620
Size: 552 957 424 86 688 317 258 825
Cuts at: 4620 5112 5502 5894 513
Size: 492 390 392 3074
Fragments arranged by size:
3074 957 825 688 552 492 424 392
390 317 258 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 4358 4460 5359 6482 6499 6652
Size: 1255 1836 942 102 899 1123 17 153
Cuts at: 6652 6837 7282 325
Size: 185 445 1498
Fragments arranged by size:
1836 1498 1255 1123 942 899 445 185
153 102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 3672 4842
Size: 274 80 33 51 1443 180 1515 1170
Cuts at: 4842 6701 8413 96
Size: 1859 1712 138
Fragments arranged by size:
1859 1712 1515 1443 1170 274 180 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3725 3757 8415 8447 3125
Size: 32 568 32 4658 32 3133
Fragments arranged by size:
4658 3133 568 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3640 1316
Size: 2324 6131
XbaI T'CTAG_A
Cuts at: 5791 5791
Size: 8455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3848 4171 4582 5110 3848
Size: 323 411 528 7193
Fragments arranged by size:
7193 528 411 323
XhoI C'TCGA_G
Cuts at: 1943 2153 1943
Size: 210 8245
XmaI C'CCGG_G
Cuts at: 1870 2286 2720 4352 4699 4931 4959 5241 5355
Size: 416 434 1632 347 232 28 282 114
Cuts at: 5355 5780 1870
Size: 425 4545
Fragments arranged by size:
4545 1632 434 425 416 347 282 232
114 28
XmnI GAAnn'nnTTC
Cuts at: 6584 6584
Size: 8455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6263 276
Size: 53 83 186 936 4729 2468
Fragments arranged by size:
4729 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SacI SanDI SapI
SfoI SrfI SwaI