Restriction map for
pFN21ASDA2038
(Circular) (Six-base) MAPSORT of: pFN21ASDA2038.seq Check: 7174 from: 1 to: 7351
pFN21ASDA2038 7351 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5161 278
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4694 1937
Size: 2757 4594
Acc65I G'GTAC_C
Cuts at: 4672 4672
Size: 7351
AclI AA'CG_TT
Cuts at: 1563 5478 5851 1563
Size: 3915 373 3063
Fragments arranged by size:
3915 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2314 2380 2716 3933 4278 5414 6798 7066
Size: 682 66 336 1217 345 1136 1384 268
Cuts at: 7066 1632
Size: 1917
Fragments arranged by size:
1917 1384 1217 1136 682 345 336 268
66
AfeI AGC'GCT
Cuts at: 2525 3074 3890 2525
Size: 549 816 5986
Fragments arranged by size:
5986 816 549
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7154
AflIII A'CryG_T
Cuts at: 1203 6250 7227 1203
Size: 5047 977 1327
Fragments arranged by size:
5047 1327 977
AgeI A'CCGG_T
Cuts at: 6182 6182
Size: 7351
AhdI GACnn_n'nnGTC
Cuts at: 6080 6080
Size: 7351
AleI CACnn'nnGTG
Cuts at: 1166 3101 1166
Size: 1935 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4446 4562 1184
Size: 3262 116 3973
Fragments arranged by size:
3973 3262 116
AlwNI CAG_nnn'CTG
Cuts at: 2100 3010 4163 5094 6666 2100
Size: 910 1153 931 1572 2785
Fragments arranged by size:
2785 1572 1153 931 910
ApaI G_GGCC'C
Cuts at: 2247 2951 3336 3781 3972 2247
Size: 704 385 445 191 5626
Fragments arranged by size:
5626 704 445 385 191
ApaLI G'TGCA_C
Cuts at: 5408 6564 5408
Size: 1156 6195
ApoI r'AATT_y
Cuts at: 1493 4660 4886 5038 7147 1493
Size: 3167 226 152 2109 1697
Fragments arranged by size:
3167 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5905 160
Size: 872 4873 1606
Fragments arranged by size:
4873 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 3185 3275 3335 3495 3774 3982
Size: 73 358 884 90 60 160 279 208
Cuts at: 3982 4316 4377 4518 4676 1870
Size: 334 61 141 158 4545
Fragments arranged by size:
4545 884 358 334 279 208 160 158
141 90 73 61 60
BamHI G'GATC_C
Cuts at: 4681 5048 4681
Size: 367 6984
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2636 2827 2953 3112
Size: 325 217 627 282 567 191 126 159
Cuts at: 3112 4350 4672 6127 618
Size: 1238 322 1455 1842
Fragments arranged by size:
1842 1455 1238 627 567 325 322 282
217 191 159 126
BanII G_rGCy'C
Cuts at: 1391 2247 2475 2951 3126 3192 3257 3282 3336
Size: 856 228 476 175 66 65 25 54
Cuts at: 3336 3366 3781 3910 3972 4122 4188 4491 4670
Size: 30 415 129 62 150 66 303 179
Cuts at: 4670 1391
Size: 4072
Fragments arranged by size:
4072 856 476 415 303 228 179 175
150 129 66 66 65 62 54 30
25
BbeI G_GCGC'C
Cuts at: 2073 2640 2957 3116 4354 2073
Size: 567 317 159 1238 5070
Fragments arranged by size:
5070 1238 567 317 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2350 3138 3954 928
Size: 971 451 788 816 4325
Fragments arranged by size:
4325 971 816 788 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5542 5576 1903
Size: 34 3605 34 3678
Fragments arranged by size:
3678 3605 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5243 6453 5243
Size: 1210 6141
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 2836 2946
Size: 107 122 71 631 605 373 791 110
Cuts at: 2946 3361 3776 5962 136
Size: 415 415 2186 1525
Fragments arranged by size:
2186 1525 791 631 605 415 415 373
122 110 107 71
BglII A'GATC_T
Cuts at: 4641 7346 4641
Size: 2705 4646
BlpI GC'TnA_GC
Cuts at: 4768 4768
Size: 7351
Bme1580I G_kGCm'C
Cuts at: 1790 2247 2832 2951 3336 3781 3972 5412 6568
Size: 457 585 119 385 445 191 1440 1156
Cuts at: 6568 1790
Size: 2573
Fragments arranged by size:
2573 1440 1156 585 457 445 385 191
119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3271 5082 6040
Size: 416 53 83 399 24 1854 1811 958
Cuts at: 6040 442
Size: 1753
Fragments arranged by size:
1854 1811 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3215 1056
Size: 2159 5192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4480 4480
Size: 7351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2240 2771 3411 3468 3508 3568 3596
Size: 841 160 531 640 57 40 60 28
Cuts at: 3596 4136 4140 4475 4651 6011 1239
Size: 540 4 335 176 1360 2579
Fragments arranged by size:
2579 1360 841 640 540 531 335 176
160 60 57 40 28 4
Bpu10I CC'TnA_GC
Cuts at: 2702 4164 2702
Size: 1462 5889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5476 6341 6639 5476
Size: 865 298 6188
Fragments arranged by size:
6188 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4468 6014 882
Size: 617 2969 1546 2219
Fragments arranged by size:
2969 2219 1546 617
BsaAI yAC'GTr
Cuts at: 493 1204 3250 4648 5142 493
Size: 711 2046 1398 494 2702
Fragments arranged by size:
2702 2046 1398 711 494
BsaBI GATnn'nnATC
Cuts at: 2255 7345 2255
Size: 5090 2261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2151 2637 2954
Size: 53 83 186 936 537 81 486 317
Cuts at: 2954 3113 4351 5158 5540 6175 275
Size: 159 1238 807 382 635 1451
Fragments arranged by size:
1451 1238 936 807 635 537 486 382
317 186 159 83 81 53
BsaWI w'CCGG_w
Cuts at: 5784 6182 6456 6603 7014 5784
Size: 398 274 147 411 6121
Fragments arranged by size:
6121 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 3041 3071 3077
Size: 30 165 30 1083 30 525 30 6
Cuts at: 3077 3107 3359 3389 3650 3680 1178
Size: 30 252 30 261 30 4849
Fragments arranged by size:
4849 1083 525 261 252 165 30 30
30 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2357 3326 3473 3592 3849 4054 4069
Size: 1051 120 969 147 119 257 205 15
Cuts at: 4069 4072 4075 4341 4405 4472 1186
Size: 3 3 266 64 67 4065
Fragments arranged by size:
4065 1051 969 266 257 205 147 120
119 67 64 15 3 3
BseYI C'CCAG_C
Cuts at: 2554 2596 2831 2923 3578 3727 3761 4024 4138
Size: 42 235 92 655 149 34 263 114
Cuts at: 4138 6554 7050 2554
Size: 2416 496 2855
Fragments arranged by size:
2855 2416 655 496 263 235 149 114
92 42 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2846 2945 4256 4428 4568 1163
Size: 573 1110 99 1311 172 140 3946
Fragments arranged by size:
3946 1311 1110 573 172 140 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2840 3102 3353 3517 3802
Size: 275 186 39 800 262 251 164 285
Cuts at: 3802 4801 5562 6590 1540
Size: 999 761 1028 2301
Fragments arranged by size:
2301 1028 999 800 761 285 275 262
251 186 164 39
BsiHKAI G_wGCw'C
Cuts at: 2475 2778 3126 3910 4333 5412 5497 6568 2475
Size: 303 348 784 423 1079 85 1071 3258
Fragments arranged by size:
3258 1079 1071 784 423 348 303 85
BsmI GAATG_Cn'
Cuts at: 4862 4955 4862
Size: 93 7258
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2475 2778 2832 2951 3126 3192
Size: 399 457 228 303 54 119 175 66
Cuts at: 3192 3257 3282 3336 3366 3781 3910 3972 4122
Size: 65 25 54 30 415 129 62 150
Cuts at: 4122 4188 4333 4491 4670 5412 5497 6568 1391
Size: 66 145 158 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 415 399 303 228
179 175 158 150 145 129 119 85
66 66 65 62 54 54 30 25
BspEI T'CCGG_A
Cuts at: 7014 7014
Size: 7351
BspHI T'CATG_A
Cuts at: 5240 6970 5240
Size: 1730 5621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2868 4588 4706 844
Size: 2024 1720 118 3489
Fragments arranged by size:
3489 2024 1720 118
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 3798 5238 1394
Size: 429 257 1718 1440 3507
Fragments arranged by size:
3507 1718 1440 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 2881 3511 5846 6020 65
Size: 1183 1633 630 2335 174 1396
Fragments arranged by size:
2335 1633 1396 1183 630 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2187 2644 2812 2835 3217 3227
Size: 110 238 139 457 168 23 382 10
Cuts at: 3227 3516 3565 5995 6182 1700
Size: 289 49 2430 187 2869
Fragments arranged by size:
2869 2430 457 382 289 238 187 168
139 110 49 23 10
BsrGI T'GTAC_A
Cuts at: 96 2442 96
Size: 2346 5005
BssHII G'CGCG_C
Cuts at: 1925 2785 3153 3155 3178 1925
Size: 860 368 2 23 6098
Fragments arranged by size:
6098 860 368 23 2
BssSI C'ACGA_G
Cuts at: 5411 6423 5411
Size: 1012 6339
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2782 2869 2968 3010 794
Size: 1988 87 99 42 5135
Fragments arranged by size:
5135 1988 99 87 42
BstBI TT'CG_AA
Cuts at: 2007 6155 2007
Size: 4148 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7351
BstXI CCAn_nnnn'nTGG
Cuts at: 2917 3690 4195 2917
Size: 773 505 6073
Fragments arranged by size:
6073 773 505
BstYI r'GATC_y
Cuts at: 1972 2496 3300 4641 4681 5048 5433 5450 6902
Size: 524 804 1341 40 367 385 17 1452
Cuts at: 6902 6988 7000 7346 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1341 804 524 385 367 346
86 40 17 12
Bsu36I CC'TnA_GG
Cuts at: 3940 3940
Size: 7351
BtgI C'CryG_G
Cuts at: 513 1065 2022 2613 3096 3387 513
Size: 552 957 591 483 291 4477
Fragments arranged by size:
4477 957 591 552 483 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3636 487
Size: 683 56 2410 4202
Fragments arranged by size:
4202 2410 683 56
BtsI GCAGTG_nn'
Cuts at: 2198 3640 3646 4200 4867 5661 5681 2198
Size: 1442 6 554 667 794 20 3868
Fragments arranged by size:
3868 1442 794 667 554 20 6
ClaI AT'CG_AT
Cuts at: 1554 4530 1554
Size: 2976 4375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4378 4413 529
Size: 35 3814 35 3467
Fragments arranged by size:
3814 3467 35 35
DraI TTT'AAA
Cuts at: 4655 4655
Size: 7351
DraIII CAC_nnn'GTG
Cuts at: 2782 2869 2782
Size: 87 7264
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2090 3029 3399 6358 809
Size: 507 774 939 370 2959 1802
Fragments arranged by size:
2959 1802 939 774 507 370
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2046 2349
Size: 54 1425 50 275 18 207 9 303
Cuts at: 2349 2574 2837 3015 3099 3350 3514 4798 5687
Size: 225 263 178 84 251 164 1284 889
Cuts at: 5687 7316 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1284 889 303 275 263 251
225 207 178 164 84 54 50 43
18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2837 3099 3350 3514 4798 1537
Size: 275 225 800 262 251 164 1284 4090
Fragments arranged by size:
4090 1284 800 275 262 251 225 164
EarI CTCTTCn'nnn_
Cuts at: 1353 4093 5281 1353
Size: 2740 1188 3423
Fragments arranged by size:
3423 2740 1188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2507 5938 6312 6458 387
Size: 1064 138 918 3431 374 146 1280
Fragments arranged by size:
3431 1280 1064 918 374 146 138
EcoICRI GAG'CTC
Cuts at: 2473 3124 3908 2473
Size: 651 784 5916
Fragments arranged by size:
5916 784 651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2240 2314 2380 2716 2771 3411
Size: 393 448 160 74 66 336 55 640
Cuts at: 3411 3468 3508 3568 3596 3933 4136 4140 4278
Size: 57 40 60 28 337 203 4 138
Cuts at: 4278 4475 4651 5414 6011 6798 7066 1239
Size: 197 176 763 597 787 268 1524
Fragments arranged by size:
1524 787 763 640 597 448 393 337
336 268 203 197 176 160 138 74
66 60 57 55 40 28 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2222 2294 4457 2222
Size: 72 2163 5116
Fragments arranged by size:
5116 2163 72
EcoO109I rG'GnC_Cy
Cuts at: 2947 3007 3022 3332 3340 3778 3944 3986 4279
Size: 60 15 310 8 438 166 42 293
Cuts at: 4279 4313 4361 2947
Size: 34 48 5937
Fragments arranged by size:
5937 438 310 293 166 60 48 42
34 15 8
EcoRI G'AATT_C
Cuts at: 4660 5038 4660
Size: 378 6973
FseI GG_CCGG'CC
Cuts at: 2052 2052
Size: 7351
FspI TGC'GCA
Cuts at: 5857 5857
Size: 7351
HaeII r_GCGC'y
Cuts at: 1678 2073 2527 2573 2640 2936 2957 3040 3057
Size: 395 454 46 67 296 21 83 17
Cuts at: 3057 3076 3116 3218 3419 3892 4354 6498 1678
Size: 19 40 102 201 473 462 2144 2531
Fragments arranged by size:
2531 2144 473 462 454 395 296 201
102 83 67 46 40 21 19 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 3077 3109 3359
Size: 32 135 32 1469 32 4 32 250
Cuts at: 3359 3391 3650 3682 4400 4432 4512 4544 5992
Size: 32 259 32 718 32 80 32 1448
Cuts at: 5992 6024 6066 6098 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1469 1448 718 259 250 135 80
42 32 32 32 32 32 32 32
32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4695 4941 669
Size: 988 281 2757 246 3079
Fragments arranged by size:
3079 2757 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7351
HpaI GTT'AAC
Cuts at: 4941 4941
Size: 7351
KasI G'GCGC_C
Cuts at: 2069 2636 2953 3112 4350 2069
Size: 567 317 159 1238 5070
Fragments arranged by size:
5070 1238 567 317 159
KpnI G_GTAC'C
Cuts at: 4676 4676
Size: 7351
MfeI C'AATT_G
Cuts at: 4950 4950
Size: 7351
MluI A'CGCG_T
Cuts at: 7227 7227
Size: 7351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6465 6649 1305
Size: 5160 184 2007
Fragments arranged by size:
5160 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 5010
Fragments arranged by size:
5010 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 2480 2675 3101 4544 5309 5668
Size: 648 1209 105 195 426 1443 765 359
Cuts at: 5668 5827 518
Size: 159 2042
Fragments arranged by size:
2042 1443 1209 765 648 426 359 195
159 105
MspA1I CmG'CkG
Cuts at: 1606 2100 2615 2749 2959 3119 3389 3513 3852
Size: 494 515 134 210 160 270 124 339
Cuts at: 3852 3953 4052 4301 4767 5444 6592 6837 1606
Size: 101 99 249 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 515 494 466 339 270
249 245 210 160 134 124 101 99
NaeI GCC'GGC
Cuts at: 2050 2189 2837 3219 3229 3518 2050
Size: 139 648 382 10 289 5883
Fragments arranged by size:
5883 648 382 289 139 10
NarI GG'CG_CC
Cuts at: 2070 2637 2954 3113 4351 2070
Size: 567 317 159 1238 5070
Fragments arranged by size:
5070 1238 567 317 159
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5842
Fragments arranged by size:
5842 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7351
NgoMIV G'CCGG_C
Cuts at: 2048 2187 2835 3217 3227 3516 2048
Size: 139 648 382 10 289 5883
Fragments arranged by size:
5883 648 382 289 139 10
NheI G'CTAG_C
Cuts at: 1052 3211 1052
Size: 2159 5192
NotI GC'GGCC_GC
Cuts at: 4798 4798
Size: 7351
NspI r_CATG'y
Cuts at: 1132 1229 2462 4709 6254 1132
Size: 97 1233 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 1233 97
PasI CC'CwG_GG
Cuts at: 1348 2273 3359 1348
Size: 925 1086 5340
Fragments arranged by size:
5340 1086 925
PciI A'CATG_T
Cuts at: 6250 6250
Size: 7351
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7351
PfoI T'CCnGG_A
Cuts at: 2289 3612 4113 2289
Size: 1323 501 5527
Fragments arranged by size:
5527 1323 501
PmeI GTTT'AAAC
Cuts at: 4655 4655
Size: 7351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 6933 6965 5420
Size: 32 1481 32 5806
Fragments arranged by size:
5806 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3022 3986 4279 4313 3022
Size: 964 293 34 6060
Fragments arranged by size:
6060 964 293 34
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7351
PsiI TTA'TAA
Cuts at: 4921 4921
Size: 7351
PspOMI G'GGCC_C
Cuts at: 2243 2947 3332 3777 3968 2243
Size: 704 385 445 191 5626
Fragments arranged by size:
5626 704 445 385 191
PstI C_TGCA'G
Cuts at: 830 2268 2760 3564 4311 4599 4703 830
Size: 1438 492 804 747 288 104 3478
Fragments arranged by size:
3478 1438 804 747 492 288 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7351
PvuII CAG'CTG
Cuts at: 3852 4052 4301 3852
Size: 200 249 6902
Fragments arranged by size:
6902 249 200
SacI G_AGCT'C
Cuts at: 2475 3126 3910 2475
Size: 651 784 5916
Fragments arranged by size:
5916 784 651
SacII CC_GC'GG
Cuts at: 2616 3390 2616
Size: 774 6577
SalI G'TCGA_C
Cuts at: 1936 4693 1936
Size: 2757 4594
SanDI GG'GwC_CC
Cuts at: 3022 4313 3022
Size: 1291 6060
SbfI CC_TGCA'GG
Cuts at: 4703 4703
Size: 7351
ScaI AGT'ACT
Cuts at: 3092 5599 7311 3092
Size: 2507 1712 3132
Fragments arranged by size:
3132 2507 1712
SexAI A'CCwGG_T
Cuts at: 4275 4275
Size: 7351
SfcI C'TryA_G
Cuts at: 826 1045 2264 2756 3560 4307 4595 4699 5834
Size: 219 1219 492 804 747 288 104 1135
Cuts at: 5834 6515 6706 826
Size: 681 191 1471
Fragments arranged by size:
1471 1219 1135 804 747 681 492 288
219 191 104
SfiI GGCCn_nnn'nGGCC
Cuts at: 2045 3776 2045
Size: 1731 5620
SfoI GGC'GCC
Cuts at: 2071 2638 2955 3114 4352 2071
Size: 567 317 159 1238 5070
Fragments arranged by size:
5070 1238 567 317 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
SgrAI Cr'CCGG_yG
Cuts at: 1700 3227 1700
Size: 1527 5824
SmaI CCC'GGG
Cuts at: 1872 2303 3187 3497 3776 4379 4678 1872
Size: 431 884 310 279 603 299 4545
Fragments arranged by size:
4545 884 603 431 310 299 279
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5455 6356 6618 820
Size: 197 926 3512 901 262 1553
Fragments arranged by size:
3512 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4648 5142 493
Size: 4155 494 2702
Fragments arranged by size:
4155 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7351
SphI G_CATG'C
Cuts at: 1132 2462 4709 1132
Size: 1330 2247 3774
Fragments arranged by size:
3774 2247 1330
SspI AAT'ATT
Cuts at: 5 52 5275 5
Size: 47 5223 2081
Fragments arranged by size:
5223 2081 47
StuI AGG'CCT
Cuts at: 1517 3770 4043 4247 1517
Size: 2253 273 204 4621
Fragments arranged by size:
4621 2253 273 204
StyI C'CwwG_G
Cuts at: 513 1065 2022 2125 2517 4242 4345 4790 513
Size: 552 957 103 392 1725 103 445 3074
Fragments arranged by size:
3074 1725 957 552 445 392 103 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5378 5395 5548 5733 6178 325
Size: 1255 3798 17 153 185 445 1498
Fragments arranged by size:
3798 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 3090 5597
Size: 274 80 33 51 1443 465 648 2507
Cuts at: 5597 7309 96
Size: 1712 138
Fragments arranged by size:
2507 1712 1443 648 465 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 3970 4002 7311 7343 2486
Size: 32 1452 32 3309 32 2494
Fragments arranged by size:
3309 2494 1452 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7351
XbaI T'CTAG_A
Cuts at: 4687 4687
Size: 7351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 7351
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7351
XmaI C'CCGG_G
Cuts at: 1870 2301 3185 3495 3774 4377 4676 1870
Size: 431 884 310 279 603 299 4545
Fragments arranged by size:
4545 884 603 431 310 299 279
XmnI GAAnn'nnTTC
Cuts at: 5480 5480
Size: 7351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5159 276
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SrfI SwaI