Restriction map for
pFN21ASDA2005
(Circular) (Six-base) MAPSORT of: pFN21ASDA2005.seq Check: 2314 from: 1 to: 9481
pFN21ASDA2005 9481 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7291 278
Size: 53 83 186 936 5755 2468
Fragments arranged by size:
5755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4455 5248 5400 5867 6824 1937
Size: 2518 793 152 467 957 4594
Fragments arranged by size:
4594 2518 957 793 467 152
Acc65I G'GTAC_C
Cuts at: 4262 6802 4262
Size: 2540 6941
AclI AA'CG_TT
Cuts at: 1563 2230 7608 7981 1563
Size: 667 5378 373 3063
Fragments arranged by size:
5378 3063 667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3183 3573 3660 3930 4125 4291 4661 5639
Size: 1551 390 87 270 195 166 370 978
Cuts at: 5639 6382 6630 7544 8928 9196 1632
Size: 743 248 914 1384 268 1917
Fragments arranged by size:
1917 1551 1384 978 914 743 390 370
270 268 248 195 166 87
AflII C'TTAA_G
Cuts at: 820 1017 2102 820
Size: 197 1085 8199
Fragments arranged by size:
8199 1085 197
AflIII A'CryG_T
Cuts at: 1203 3459 5592 8380 9357 1203
Size: 2256 2133 2788 977 1327
Fragments arranged by size:
2788 2256 2133 1327 977
AgeI A'CCGG_T
Cuts at: 8312 8312
Size: 9481
AhdI GACnn_n'nnGTC
Cuts at: 8210 8210
Size: 9481
AleI CACnn'nnGTG
Cuts at: 1166 2651 4973 1166
Size: 1485 2322 5674
Fragments arranged by size:
5674 2322 1485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9481
AlwNI CAG_nnn'CTG
Cuts at: 7224 8796 7224
Size: 1572 7909
ApaLI G'TGCA_C
Cuts at: 7538 8694 7538
Size: 1156 8325
ApoI r'AATT_y
Cuts at: 1493 2127 2359 2669 2710 3000 3127 3647 4046
Size: 634 232 310 41 290 127 520 399
Cuts at: 4046 5077 5111 5368 5797 6166 6532 6790 7016
Size: 1031 34 257 429 369 366 258 226
Cuts at: 7016 7168 9277 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1031 634 520 429 399 369
366 310 290 258 257 232 226 152
127 41 34
AseI AT'TA_AT
Cuts at: 160 1032 2109 5195 8035 160
Size: 872 1077 3086 2840 1606
Fragments arranged by size:
3086 2840 1606 1077 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9481
AvaI C'yCGr_G
Cuts at: 1870 1943 6806 1870
Size: 73 4863 4545
Fragments arranged by size:
4863 4545 73
AvrII C'CTAG_G
Cuts at: 6727 6727
Size: 9481
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4957 4990 4957
Size: 33 9448
BamHI G'GATC_C
Cuts at: 5953 6811 7178 5953
Size: 858 367 8256
Fragments arranged by size:
8256 858 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 4262 4631 6802 8257
Size: 325 217 627 939 1536 369 2171 1455
Cuts at: 8257 618
Size: 1842
Fragments arranged by size:
2171 1842 1536 1455 939 627 369 325
217
BanII G_rGCy'C
Cuts at: 1391 6800 1391
Size: 5409 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 2677 3450 4911 928
Size: 971 204 574 773 1461 5498
Fragments arranged by size:
5498 1461 971 773 574 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7672 7706 1903
Size: 34 5735 34 3678
Fragments arranged by size:
5735 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4111 7373 8583 4111
Size: 3262 1210 5009
Fragments arranged by size:
5009 3262 1210
BclI T'GATC_A
Cuts at: 1548 2527 2823 3375 4365 4692 1548
Size: 979 296 552 990 327 6337
Fragments arranged by size:
6337 990 979 552 327 296
BfrBI ATG'CAT
Cuts at: 4279 5267 4279
Size: 988 8493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8092 136
Size: 107 122 71 631 605 6420 1525
Fragments arranged by size:
6420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3796 5809 9476 3796
Size: 2013 3667 3801
Fragments arranged by size:
3801 3667 2013
BlpI GC'TnA_GC
Cuts at: 6898 6898
Size: 9481
Bme1580I G_kGCm'C
Cuts at: 1790 5341 7542 8698 1790
Size: 3551 2201 1156 2573
Fragments arranged by size:
3551 2573 2201 1156
BmgBI CAC'GTC
Cuts at: 1318 5342 1318
Size: 4024 5457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5344 7212 8170
Size: 416 53 83 399 24 3927 1868 958
Cuts at: 8170 442
Size: 1753
Fragments arranged by size:
3927 1868 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3066 1056
Size: 2010 7471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3716
Size: 9481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4073 5975 8141 1239
Size: 2834 1902 2166 2579
Fragments arranged by size:
2834 2579 2166 1902
Bpu10I CC'TnA_GC
Cuts at: 5284 5284
Size: 9481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5254 5485 7606 8471 8769 5254
Size: 231 2121 865 298 5966
Fragments arranged by size:
5966 2121 865 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8144 882
Size: 617 6645 2219
Fragments arranged by size:
6645 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6778 7272 493
Size: 711 5574 494 2702
Fragments arranged by size:
5574 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2385 2695 9475 2385
Size: 310 6780 2391
Fragments arranged by size:
6780 2391 310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7288 7670 8305 275
Size: 53 83 186 936 5755 382 635 1451
Fragments arranged by size:
5755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7914 8312 8586 8733 9144 7914
Size: 398 274 147 411 8251
Fragments arranged by size:
8251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2754 2784 2870 2900 5722
Size: 30 165 30 1351 30 86 30 2822
Cuts at: 5722 5752 1178
Size: 30 4907
Fragments arranged by size:
4907 2822 1351 165 86 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 6011 1186
Size: 4825 4656
BseYI C'CCAG_C
Cuts at: 3500 4194 8684 9180 3500
Size: 694 4490 496 3801
Fragments arranged by size:
4490 3801 694 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3969 5259 6442 1163
Size: 573 2233 1290 1183 4202
Fragments arranged by size:
4202 2233 1290 1183 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6931 7692 8720 1540
Size: 275 186 4930 761 1028 2301
Fragments arranged by size:
4930 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2590 7542 7627 8698 2590
Size: 4952 85 1071 3373
Fragments arranged by size:
4952 3373 1071 85
BsmI GAATG_Cn'
Cuts at: 5267 6992 7085 5267
Size: 1725 93 7663
Fragments arranged by size:
7663 1725 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5575 5575
Size: 9481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2590 5341 6800 7542 7627 8698 1391
Size: 399 800 2751 1459 742 85 1071 2174
Fragments arranged by size:
2751 2174 1459 1071 800 742 399 85
BspEI T'CCGG_A
Cuts at: 9144 9144
Size: 9481
BspHI T'CATG_A
Cuts at: 2275 4689 4695 7370 9100 2275
Size: 2414 6 2675 1730 2656
Fragments arranged by size:
2675 2656 2414 1730 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6414 6836 844
Size: 5570 422 3489
Fragments arranged by size:
5570 3489 422
BsrBI CCG'CTC
Cuts at: 1394 1823 7368 1394
Size: 429 5545 3507
Fragments arranged by size:
5545 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2683 3192 7976 8150 65
Size: 1183 1435 509 4784 174 1396
Fragments arranged by size:
4784 1435 1396 1183 509 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8125 8312 1700
Size: 110 6315 187 2869
Fragments arranged by size:
6315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4013 96
Size: 3917 5564
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9481
BssSI C'ACGA_G
Cuts at: 7541 8553 7541
Size: 1012 8469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2131 4352 794
Size: 1337 2221 5923
Fragments arranged by size:
5923 2221 1337
BstBI TT'CG_AA
Cuts at: 2007 8285 2007
Size: 6278 3203
BstEII G'GTnAC_C
Cuts at: 1184 4375 5562 1184
Size: 3191 1187 5103
Fragments arranged by size:
5103 3191 1187
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 3570 2653
Size: 917 8564
BstYI r'GATC_y
Cuts at: 1972 3694 3796 3938 4544 5809 5953 6392 6811
Size: 1722 102 142 606 1265 144 439 419
Cuts at: 6811 7178 7563 7580 9032 9118 9130 9476 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1722 1452 1265 606 439 419 385
367 346 144 142 102 86 17 12
BstZ17I GTA'TAC
Cuts at: 5868 5868
Size: 9481
BtgI C'CryG_G
Cuts at: 513 1065 2204 2646 2758 3934 6090 513
Size: 552 1139 442 112 1176 2156 3904
Fragments arranged by size:
3904 2156 1176 1139 552 442 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2531 487
Size: 683 56 1305 7437
Fragments arranged by size:
7437 1305 683 56
BtsI GCAGTG_nn'
Cuts at: 4189 6997 7791 7811 4189
Size: 2808 794 20 5859
Fragments arranged by size:
5859 2808 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2871 2906 529
Size: 35 2307 35 7104
Fragments arranged by size:
7104 2307 35 35
DraI TTT'AAA
Cuts at: 3132 3327 3858 6785 3132
Size: 195 531 2927 5828
Fragments arranged by size:
5828 2927 531 195
DraIII CAC_nnn'GTG
Cuts at: 4847 4847
Size: 9481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2930 8488 809
Size: 507 1614 5558 1802
Fragments arranged by size:
5558 1802 1614 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3081 6177 6326
Size: 54 1425 50 275 18 1251 3096 149
Cuts at: 6326 6928 7817 9446 8
Size: 602 889 1629 43
Fragments arranged by size:
3096 1629 1425 1251 889 602 275 149
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6928 1537
Size: 275 5116 4090
Fragments arranged by size:
5116 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3162 3679 5067 5098 5139 7411 1353
Size: 1809 517 1388 31 41 2272 3423
Fragments arranged by size:
3423 2272 1809 1388 517 41 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8068 8442 8588 387
Size: 1064 138 6479 374 146 1280
Fragments arranged by size:
6479 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3183 3573 3660 3930 4073 4125 4291
Size: 393 1551 390 87 270 143 52 166
Cuts at: 4291 4661 5639 5975 6382 6630 7544 8141 8928
Size: 370 978 336 407 248 914 597 787
Cuts at: 8928 9196 1239
Size: 268 1524
Fragments arranged by size:
1551 1524 978 914 787 597 407 393
390 370 336 270 268 248 166 143
87 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2580 2580
Size: 9481
EcoO109I rG'GnC_Cy
Cuts at: 2182 6296 6746 2182
Size: 4114 450 4917
Fragments arranged by size:
4917 4114 450
EcoRI G'AATT_C
Cuts at: 2710 6790 7168 2710
Size: 4080 378 5023
Fragments arranged by size:
5023 4080 378
EcoRV GAT'ATC
Cuts at: 2407 3076 2407
Size: 669 8812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 4321 5651 6735 2815
Size: 1506 1330 1084 5561
Fragments arranged by size:
5561 1506 1330 1084
FspI TGC'GCA
Cuts at: 7987 7987
Size: 9481
HaeII r_GCGC'y
Cuts at: 1678 8628 1678
Size: 6950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3353 3385 3463 3495 5546
Size: 32 135 32 1781 32 78 32 2051
Cuts at: 5546 5578 5722 5754 8122 8154 8196 8228 1373
Size: 32 144 32 2368 32 42 32 2626
Fragments arranged by size:
2626 2368 2051 1781 144 135 78 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4765 6825 7071 669
Size: 988 281 2827 2060 246 3079
Fragments arranged by size:
3079 2827 2060 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3393 4172 4897 5202 748
Size: 2645 779 725 305 5027
Fragments arranged by size:
5027 2645 779 725 305
HpaI GTT'AAC
Cuts at: 7071 7071
Size: 9481
KpnI G_GTAC'C
Cuts at: 4266 6806 4266
Size: 2540 6941
MfeI C'AATT_G
Cuts at: 2501 7080 2501
Size: 4579 4902
MluI A'CGCG_T
Cuts at: 9357 9357
Size: 9481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3333 5406 8595 8779 1305
Size: 2028 2073 3189 184 2007
Fragments arranged by size:
3189 2073 2028 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3083 6179 6328 10
Size: 54 1425 343 1251 3096 149 3163
Fragments arranged by size:
3163 3096 1425 1251 343 149 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 2663 2814 2993 3568 4412 4694
Size: 648 1485 12 151 179 575 844 282
Cuts at: 4694 4973 7439 7798 7957 518
Size: 279 2466 359 159 2042
Fragments arranged by size:
2466 2042 1485 844 648 575 359 282
279 179 159 151 12
MspA1I CmG'CkG
Cuts at: 1606 3504 6897 7574 8722 8967 1606
Size: 1898 3393 677 1148 245 2120
Fragments arranged by size:
3393 2120 1898 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2204 2758 6090 513
Size: 552 1139 554 3332 3904
Fragments arranged by size:
3904 3332 1139 554 552
NdeI CA'TA_TG
Cuts at: 387 5703 6757 387
Size: 5316 1054 3111
Fragments arranged by size:
5316 3111 1054
NheI G'CTAG_C
Cuts at: 1052 3062 1052
Size: 2010 7471
NotI GC'GGCC_GC
Cuts at: 6928 6928
Size: 9481
NsiI A_TGCA'T
Cuts at: 4281 5269 4281
Size: 988 8493
NspI r_CATG'y
Cuts at: 1132 1229 2078 3463 4283 5596 6421 6839 8384
Size: 97 849 1385 820 1313 825 418 1545
Cuts at: 8384 1132
Size: 2229
Fragments arranged by size:
2229 1545 1385 1313 849 825 820 418
97
PasI CC'CwG_GG
Cuts at: 1348 4884 1348
Size: 3536 5945
PciI A'CATG_T
Cuts at: 3459 5592 8380 3459
Size: 2133 2788 4560
Fragments arranged by size:
4560 2788 2133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4256 1108
Size: 3148 6333
PfoI T'CCnGG_A
Cuts at: 2281 4050 4547 5991 2281
Size: 1769 497 1444 5771
Fragments arranged by size:
5771 1769 1444 497
PmeI GTTT'AAAC
Cuts at: 6785 6785
Size: 9481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7550 7582 9063 9095 7550
Size: 32 1481 32 7936
Fragments arranged by size:
7936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 6296 2182
Size: 4114 5367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9481
PsiI TTA'TAA
Cuts at: 7051 7051
Size: 9481
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4909 4941 4909
Size: 32 9449
PstI C_TGCA'G
Cuts at: 830 2166 2907 6129 6833 830
Size: 1336 741 3222 704 3478
Fragments arranged by size:
3478 3222 1336 741 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9481
PvuII CAG'CTG
Cuts at: 3504 3504
Size: 9481
SalI G'TCGA_C
Cuts at: 1936 6823 1936
Size: 4887 4594
SbfI CC_TGCA'GG
Cuts at: 2166 6833 2166
Size: 4667 4814
ScaI AGT'ACT
Cuts at: 6509 7729 9441 6509
Size: 1220 1712 6549
Fragments arranged by size:
6549 1712 1220
SfcI C'TryA_G
Cuts at: 826 1045 2162 2903 4381 5443 6125 6627 6829
Size: 219 1117 741 1478 1062 682 502 202
Cuts at: 6829 7964 8645 8836 826
Size: 1135 681 191 1471
Fragments arranged by size:
1478 1471 1135 1117 1062 741 682 681
502 219 202 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9481
SmaI CCC'GGG
Cuts at: 1872 6808 1872
Size: 4936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2102 5233 5500 7585 8486 8748
Size: 197 926 159 3131 267 2085 901 262
Cuts at: 8748 820
Size: 1553
Fragments arranged by size:
3131 2085 1553 926 901 267 262 197
159
SnaBI TAC'GTA
Cuts at: 493 6778 7272 493
Size: 6285 494 2702
Fragments arranged by size:
6285 2702 494
SpeI A'CTAG_T
Cuts at: 152 4874 152
Size: 4722 4759
SphI G_CATG'C
Cuts at: 1132 6839 1132
Size: 5707 3774
SspI AAT'ATT
Cuts at: 5 52 2377 4218 4854 7405 5
Size: 47 2325 1841 636 2551 2081
Fragments arranged by size:
2551 2325 2081 1841 636 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9481
StyI C'CwwG_G
Cuts at: 513 1065 2204 2758 2882 4611 6090 6727 6920
Size: 552 1139 554 124 1729 1479 637 193
Cuts at: 6920 513
Size: 3074
Fragments arranged by size:
3074 1729 1479 1139 637 554 552 193
124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3897 5606 7508 7525 7678 7863 8308
Size: 1255 2317 1709 1902 17 153 185 445
Cuts at: 8308 325
Size: 1498
Fragments arranged by size:
2317 1902 1709 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 4877 5004
Size: 274 80 33 51 1443 2036 864 127
Cuts at: 5004 6408 6507 7727 9439 96
Size: 1404 99 1220 1712 138
Fragments arranged by size:
2036 1712 1443 1404 1220 864 274 138
127 99 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3833 3865 3978 4010 9441 9473 3833
Size: 32 113 32 5431 32 3841
Fragments arranged by size:
5431 3841 113 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3907 6279 1316
Size: 2591 2372 4518
Fragments arranged by size:
4518 2591 2372
XbaI T'CTAG_A
Cuts at: 3149 3556 6817 3149
Size: 407 3261 5813
Fragments arranged by size:
5813 3261 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5701 5701
Size: 9481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9481
XmaI C'CCGG_G
Cuts at: 1870 6806 1870
Size: 4936 4545
XmnI GAAnn'nnTTC
Cuts at: 2424 2898 4304 4921 5081 7610 2424
Size: 474 1406 617 160 2529 4295
Fragments arranged by size:
4295 2529 1406 617 474 160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7289 276
Size: 53 83 186 936 5755 2468
Fragments arranged by size:
5755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BsiWI
Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI