Restriction map for
pFN21ASDA2003
(Circular) (Six-base) MAPSORT of: pFN21ASDA2003.seq Check: 9990 from: 1 to: 6040
pFN21ASDA2003 6040 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2300 2300
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4103 5487 5755 1632
Size: 2471 1384 268 1917
Fragments arranged by size:
2471 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaLI G'TGCA_C
Cuts at: 3265 4097 5253 3265
Size: 832 1156 4052
Fragments arranged by size:
4052 1156 832
ApoI r'AATT_y
Cuts at: 1493 3211 3321 3349 3575 3727 5836 1493
Size: 1718 110 28 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 226 152 110 28
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 1870
Size: 73 1422 4545
Fragments arranged by size:
4545 1422 73
BamHI G'GATC_C
Cuts at: 2912 3370 3737 2912
Size: 458 367 5215
Fragments arranged by size:
5215 458 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3359 1391
Size: 1968 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2261 928
Size: 971 362 4707
Fragments arranged by size:
4707 971 362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2060 3269 4101 5257 1790
Size: 270 1209 832 1156 2573
Fragments arranged by size:
2573 1209 1156 832 270
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2367 2751 2835
Size: 416 53 83 399 24 950 384 84
Cuts at: 2835 3087 3255 3771 4729 442
Size: 252 168 516 958 1753
Fragments arranged by size:
1753 958 950 516 416 399 384 252
168 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2595 3015 3115 3283 4700 1239
Size: 935 421 420 100 168 1417 2579
Fragments arranged by size:
2579 1417 935 421 420 168 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 4703 882
Size: 617 615 2589 2219
Fragments arranged by size:
2589 2219 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 3337 3831 493
Size: 711 2133 494 2702
Fragments arranged by size:
2702 2133 711 494
BsaBI GATnn'nnATC
Cuts at: 2187 6034 2187
Size: 3847 2193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3847 4229 4864
Size: 53 83 186 936 512 1802 382 635
Cuts at: 4864 275
Size: 1451
Fragments arranged by size:
1802 1451 936 635 512 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2643 4473 4871 5145 5292 5703 2643
Size: 1830 398 274 147 411 2980
Fragments arranged by size:
2980 1830 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6040
BseYI C'CCAG_C
Cuts at: 5243 5739 5243
Size: 496 5544
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2245 2781 2782 2866 2950 3033 3034
Size: 573 509 536 1 84 84 83 1
Cuts at: 3034 3118 3202 1163
Size: 84 84 4001
Fragments arranged by size:
4001 573 536 509 84 84 84 84
83 1 1
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3269 4101 4186 5257 3269
Size: 832 85 1071 4052
Fragments arranged by size:
4052 1071 832 85
BsmI GAATG_Cn'
Cuts at: 2444 3551 3644 2444
Size: 1107 93 4840
Fragments arranged by size:
4840 1107 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 3269 3359 4101 4186 5257 1391
Size: 399 270 1209 90 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 399 270 90 85
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 2671 3929 5659 2671
Size: 1258 1730 3052
Fragments arranged by size:
3052 1730 1258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2267 2300 3395 844
Size: 1423 33 1095 3489
Fragments arranged by size:
3489 1423 1095 33
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 4535 4709 65
Size: 1183 988 2299 174 1396
Fragments arranged by size:
2299 1396 1183 988 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 2007 4844 2007
Size: 2837 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2912 3370 3737 4122 4139 5591 5677 5689
Size: 940 458 367 385 17 1452 86 12
Cuts at: 5689 6035 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 940 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4531
Fragments arranged by size:
4531 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 2655 2770 2854 3106 3190 3556 4350 4370
Size: 137 115 84 252 84 366 794 20
Cuts at: 4370 2518
Size: 4188
Fragments arranged by size:
4188 794 366 252 137 115 84 84
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2079 2319
Size: 54 1425 50 275 18 201 48 240
Cuts at: 2319 3487 4376 6005 8
Size: 1168 889 1629 43
Fragments arranged by size:
1629 1425 1168 889 275 240 201 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3970 1353
Size: 2617 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2595 3015 3115 3283 4103 4700
Size: 393 542 421 420 100 168 820 597
Cuts at: 4700 5487 5755 1239
Size: 787 268 1524
Fragments arranged by size:
1524 820 787 597 542 421 420 393
268 168 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 2117
Size: 6040
EcoRI G'AATT_C
Cuts at: 3321 3349 3727 3321
Size: 28 378 5634
Fragments arranged by size:
5634 378 28
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2035 2067 4681 4713 4755
Size: 32 135 32 463 32 2614 32 42
Cuts at: 4755 4787 1373
Size: 32 2626
Fragments arranged by size:
2626 2614 463 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 10
Size: 54 1425 343 489 3729
Fragments arranged by size:
3729 1425 489 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2676 2758 3998 4357 4516 518
Size: 648 1510 82 1240 359 159 2042
Fragments arranged by size:
2042 1510 1240 648 359 159 82
MspA1I CmG'CkG
Cuts at: 1606 2030 2451 3456 4133 5281 5526 1606
Size: 424 421 1005 677 1148 245 2120
Fragments arranged by size:
2120 1148 1005 677 424 421 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4531
Fragments arranged by size:
4531 957 552
NdeI CA'TA_TG
Cuts at: 387 2507 2755 387
Size: 2120 248 3672
Fragments arranged by size:
3672 2120 248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3009 3610 3009
Size: 601 5439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2473 2505 2473
Size: 32 6008
PstI C_TGCA'G
Cuts at: 830 2297 2662 3392 830
Size: 1467 365 730 3478
Fragments arranged by size:
3478 1467 730 365
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2293 2490 2542 2658 3124 3388 4523
Size: 219 1248 197 52 116 466 264 1135
Cuts at: 4523 5204 5395 826
Size: 681 191 1471
Fragments arranged by size:
1471 1248 1135 681 466 264 219 197
191 116 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3337 3831 493
Size: 2844 494 2702
Fragments arranged by size:
2844 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 2836 2920 3004 3088 1517
Size: 1319 84 84 84 4469
Fragments arranged by size:
4469 1319 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2022 2206 3479 513
Size: 552 957 184 1273 3074
Fragments arranged by size:
3074 1273 957 552 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4067 4084 4237 4422 4867 325
Size: 1255 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4286 5998 96
Size: 274 80 33 51 1443 2309 1712 138
Fragments arranged by size:
2309 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2897 2929 6000 6032 2897
Size: 32 3071 32 2905
Fragments arranged by size:
3071 2905 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 2179 3376 2179
Size: 1197 4843
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 2312 4169 2312
Size: 1857 4183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI