Restriction map for
pFN21ASDA1970
(Circular) (Six-base) MAPSORT of: pFN21ASDA1970.seq Check: 5933 from: 1 to: 6298
pFN21ASDA1970 6298 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2533 2590 2802 2850 2533
Size: 57 212 48 5981
Fragments arranged by size:
5981 212 57 48
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3213 3282 3641 1937
Size: 1276 69 359 4594
Fragments arranged by size:
4594 1276 359 69
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2492 3449 4361 5745 6013 1632
Size: 806 54 957 912 1384 268 1917
Fragments arranged by size:
1917 1384 957 912 806 268 54
AfeI AGC'GCT
Cuts at: 2412 2412
Size: 6298
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 3271 5027 3271
Size: 1756 4542
AleI CACnn'nnGTG
Cuts at: 1166 2175 1166
Size: 1009 5289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2931 3017 1184
Size: 1747 86 4465
Fragments arranged by size:
4465 1747 86
AlwNI CAG_nnn'CTG
Cuts at: 2461 2557 2623 2812 3106 4041 5613 2461
Size: 96 66 189 294 935 1572 3146
Fragments arranged by size:
3146 1572 935 294 189 96 66
ApaI G_GGCC'C
Cuts at: 2364 2364
Size: 6298
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 3080 3607 3833 3985 6094 1493
Size: 1587 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 1587 527 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 3623 1870
Size: 73 1680 4545
Fragments arranged by size:
4545 1680 73
BamHI G'GATC_C
Cuts at: 3225 3570 3628 3995 3225
Size: 345 58 367 5528
Fragments arranged by size:
5528 367 345 58
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2605 3129 3455 3619 5074
Size: 325 217 627 818 524 326 164 1455
Cuts at: 5074 618
Size: 1842
Fragments arranged by size:
1842 1455 818 627 524 326 325 217
164
BanII G_rGCy'C
Cuts at: 1391 2339 2364 2469 3617 1391
Size: 948 25 105 1148 4072
Fragments arranged by size:
4072 1148 948 105 25
BbeI G_GCGC'C
Cuts at: 3133 3133
Size: 6298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2426 928
Size: 971 527 4800
Fragments arranged by size:
4800 971 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 3012 1548
Size: 1464 4834
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2160 4909 136
Size: 107 122 71 631 605 488 2749 1525
Fragments arranged by size:
2749 1525 631 605 488 122 107 71
BglII A'GATC_T
Cuts at: 3386 6293 3386
Size: 2907 3391
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 2114 2364 4359 5515 1790
Size: 324 250 1995 1156 2573
Fragments arranged by size:
2573 1995 1156 324 250
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2666 3203 3272
Size: 416 53 83 399 24 1249 537 69
Cuts at: 3272 4029 4987 442
Size: 757 958 1753
Fragments arranged by size:
1753 1249 958 757 537 416 399 83
69 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3420 3420
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2483 2615 2924 3083 3086 4958 1239
Size: 1088 156 132 309 159 3 1872 2579
Fragments arranged by size:
2579 1872 1088 309 159 156 132 3
Bpu10I CC'TnA_GC
Cuts at: 2968 2968
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4423 5288 5586 4423
Size: 865 298 5135
Fragments arranged by size:
5135 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2661 4961 882
Size: 617 1162 2300 2219
Fragments arranged by size:
2300 2219 1162 617
BsaAI yAC'GTr
Cuts at: 493 1204 2752 3217 3595 4089 493
Size: 711 1548 465 378 494 2702
Fragments arranged by size:
2702 1548 711 494 465 378
BsaBI GATnn'nnATC
Cuts at: 6292 6292
Size: 6298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2501 3130 3330 4105
Size: 53 83 186 936 968 629 200 775
Cuts at: 4105 4487 5122 275
Size: 382 635 1451
Fragments arranged by size:
1451 968 936 775 635 629 382 200
186 83 53
BsaWI w'CCGG_w
Cuts at: 2423 4731 5129 5403 5550 5961 2423
Size: 2308 398 274 147 411 2760
Fragments arranged by size:
2760 2308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2658 2688 3081 3111 1178
Size: 30 165 30 1255 30 393 30 4365
Fragments arranged by size:
4365 1255 393 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2053 2789 2843 3179 1186
Size: 867 736 54 336 4305
Fragments arranged by size:
4305 867 736 336 54
BseYI C'CCAG_C
Cuts at: 2150 2357 2454 3370 5501 5997 2150
Size: 207 97 916 2131 496 2451
Fragments arranged by size:
2451 2131 916 496 207 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2542 3077 1163
Size: 573 806 535 4384
Fragments arranged by size:
4384 806 573 535
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2469 2910 3322 4359 4444 5515 2469
Size: 441 412 1037 85 1071 3252
Fragments arranged by size:
3252 1071 1037 441 412 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
BsmBI CGTCTCn'nnnn_
Cuts at: 2782 2782
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2339 2364 2469 2910 3322 3617
Size: 399 324 225 25 105 441 412 295
Cuts at: 3617 4359 4444 5515 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 412 399 324 295
225 105 85 25
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 2679 4187 5917 2679
Size: 1508 1730 3060
Fragments arranged by size:
3060 1730 1508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2533 2590 2802 2850 3142 3653 844
Size: 1689 57 212 48 292 511 3489
Fragments arranged by size:
3489 1689 511 292 212 57 48
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3121 4793 4967 65
Size: 1183 1873 1672 174 1396
Fragments arranged by size:
1873 1672 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4942 5129 1700
Size: 110 3132 187 2869
Fragments arranged by size:
3132 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2196 96
Size: 2100 4198
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 4358 5370 4358
Size: 1012 5286
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 4487
BstBI TT'CG_AA
Cuts at: 2007 5102 2007
Size: 3095 3203
BstEII G'GTnAC_C
Cuts at: 1184 3037 1184
Size: 1853 4445
BstYI r'GATC_y
Cuts at: 1972 3225 3386 3570 3628 3995 4380 4397 5849
Size: 1253 161 184 58 367 385 17 1452
Cuts at: 5849 5935 5947 6293 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1253 385 367 346 184 161
86 58 17 12
BstZ17I GTA'TAC
Cuts at: 3283 3283
Size: 6298
Bsu36I CC'TnA_GG
Cuts at: 2036 2240 2036
Size: 204 6094
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4789
Fragments arranged by size:
4789 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 2187 2781 3814 4608 4628 2187
Size: 594 1033 794 20 3857
Fragments arranged by size:
3857 1033 794 594 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2559 2652
Size: 54 1425 50 275 18 247 482 93
Cuts at: 2652 3745 4634 6263 8
Size: 1093 889 1629 43
Fragments arranged by size:
1629 1425 1093 889 482 275 247 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 4228 1353
Size: 1325 1550 3423
Fragments arranged by size:
3423 1550 1325
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2579 4885 5259 5405 387
Size: 1064 138 990 2306 374 146 1280
Fragments arranged by size:
2306 1280 1064 990 374 146 138
EcoICRI GAG'CTC
Cuts at: 2467 2467
Size: 6298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2438 2483 2492 2615 2924 3083
Size: 393 695 111 45 9 123 309 159
Cuts at: 3083 3086 3449 4361 4958 5745 6013 1239
Size: 3 363 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 695 597 393 363 309
268 159 123 111 45 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 2597 2804 3032 3161 3309 2474
Size: 123 207 228 129 148 5463
Fragments arranged by size:
5463 228 207 148 129 123
EcoO109I rG'GnC_Cy
Cuts at: 2361 2566 2620 2809 2997 3154 3515 2361
Size: 205 54 189 188 157 361 5144
Fragments arranged by size:
5144 361 205 189 188 157 54
EcoRI G'AATT_C
Cuts at: 3080 3607 3985 3080
Size: 527 378 5393
Fragments arranged by size:
5393 527 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 6298
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 2033 2414 3133 5445 1678
Size: 355 381 719 2312 2531
Fragments arranged by size:
2531 2312 719 381 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2658 2690 3081 3113 3229
Size: 32 135 32 1086 32 391 32 116
Cuts at: 3229 3257 3261 3289 3320 3352 4939 4971 5013
Size: 28 4 28 31 32 1587 32 42
Cuts at: 5013 5045 1373
Size: 32 2626
Fragments arranged by size:
2626 1587 1086 391 135 116 42 32
32 32 32 32 32 32 31 28
28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3888 669
Size: 988 281 1704 246 3079
Fragments arranged by size:
3079 1704 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 3129 3129
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2364 2925 5412 5596 1305
Size: 1059 561 2487 184 2007
Fragments arranged by size:
2487 2007 1059 561 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2561 2654 10
Size: 54 1425 343 729 93 3654
Fragments arranged by size:
3654 1425 729 343 93 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 4256 4615 4774 518
Size: 648 1009 2081 359 159 2042
Fragments arranged by size:
2081 2042 1009 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2285 2380 2458 3103 3136 3184 3325 3714
Size: 679 95 78 645 33 48 141 389
Cuts at: 3714 4391 5539 5784 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 679 677 645 389 245 141
95 78 48 33
NarI GG'CG_CC
Cuts at: 3130 3130
Size: 6298
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4789
Fragments arranged by size:
4789 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 2305 3656 5201 1132
Size: 97 1076 1351 1545 2229
Fragments arranged by size:
2229 1545 1351 1076 97
PasI CC'CwG_GG
Cuts at: 1348 2812 1348
Size: 1464 4834
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2461 2557 2623 2737 2812 3106 3139 3580
Size: 1353 96 66 114 75 294 33 441
Cuts at: 3580 1108
Size: 3826
Fragments arranged by size:
3826 1353 441 294 114 96 75 66
33
PfoI T'CCnGG_A
Cuts at: 2616 2901 2616
Size: 285 6013
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PmlI CAC'GTG
Cuts at: 2752 2752
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2566 2620 2997 3154 3515 2566
Size: 54 377 157 361 5349
Fragments arranged by size:
5349 377 361 157 54
PshAI GACnn'nnGTC
Cuts at: 1613 3035 1613
Size: 1422 4876
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PspOMI G'GGCC_C
Cuts at: 2360 2360
Size: 6298
PstI C_TGCA'G
Cuts at: 830 2052 3153 3264 3310 3650 830
Size: 1222 1101 111 46 340 3478
Fragments arranged by size:
3478 1222 1101 340 111 46
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 3136 3184 3325 3136
Size: 48 141 6109
Fragments arranged by size:
6109 141 48
SacI G_AGCT'C
Cuts at: 2469 2469
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SapI GCTCTTCn'nnn_
Cuts at: 2678 2678
Size: 6298
SbfI CC_TGCA'GG
Cuts at: 3264 3650 3264
Size: 386 5912
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SexAI A'CCwGG_T
Cuts at: 2622 2622
Size: 6298
SfcI C'TryA_G
Cuts at: 826 1045 2048 3149 3260 3306 3522 3646 4781
Size: 219 1003 1101 111 46 216 124 1135
Cuts at: 4781 5462 5653 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1101 1003 681 219 216 191
124 111 46
SfoI GGC'GCC
Cuts at: 3131 3131
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4402 5303 5565 820
Size: 197 926 2459 901 262 1553
Fragments arranged by size:
2459 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3595 4089 493
Size: 3102 494 2702
Fragments arranged by size:
3102 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SspI AAT'ATT
Cuts at: 5 52 2293 4222 5
Size: 47 2241 1929 2081
Fragments arranged by size:
2241 2081 1929 47
StuI AGG'CCT
Cuts at: 1517 2062 2483 3170 1517
Size: 545 421 687 4645
Fragments arranged by size:
4645 687 545 421
StyI C'CwwG_G
Cuts at: 513 1065 2022 2562 3543 3737 513
Size: 552 957 540 981 194 3074
Fragments arranged by size:
3074 981 957 552 540 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 4325 4342 4495 4680 5125 325
Size: 1255 585 2160 17 153 185 445 1498
Fragments arranged by size:
2160 1498 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3462 4544
Size: 274 80 33 51 1443 219 1266 1082
Cuts at: 4544 6256 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1266 1082 274 219 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6258 6290 6258
Size: 32 6266
Tth111I GACn'n_nGTC
Cuts at: 1316 2624 2672 3243 1316
Size: 1308 48 571 4371
Fragments arranged by size:
4371 1308 571 48
XbaI T'CTAG_A
Cuts at: 3379 3634 3379
Size: 255 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 3367 2707
Size: 660 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 4427 4427
Size: 6298
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI
DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI
SwaI