Restriction map for
pFN21ASDA1960
(Circular) (Six-base) MAPSORT of: pFN21ASDA1960.seq Check: 7138 from: 1 to: 7285
pFN21ASDA1960 7285 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2492 5095 278
Size: 53 83 186 936 956 2603 2468
Fragments arranged by size:
2603 2468 956 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4628 1937
Size: 2691 4594
Acc65I G'GTAC_C
Cuts at: 4606 4606
Size: 7285
AclI AA'CG_TT
Cuts at: 1563 5412 5785 1563
Size: 3849 373 3063
Fragments arranged by size:
3849 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 2899 2928 3992 4328 4445 5348 6732
Size: 809 458 29 1064 336 117 903 1384
Cuts at: 6732 7000 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1064 903 809 458 336 268
117 29
AfeI AGC'GCT
Cuts at: 2941 2941
Size: 7285
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7088
AflIII A'CryG_T
Cuts at: 1203 6184 7161 1203
Size: 4981 977 1327
Fragments arranged by size:
4981 1327 977
AgeI A'CCGG_T
Cuts at: 4366 6116 4366
Size: 1750 5535
AhdI GACnn_n'nnGTC
Cuts at: 2890 6014 2890
Size: 3124 4161
AleI CACnn'nnGTG
Cuts at: 1166 4054 1166
Size: 2888 4397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2537 3394 1184
Size: 1353 857 5075
Fragments arranged by size:
5075 1353 857
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 2870 2902 2477
Size: 32 361 32 6860
Fragments arranged by size:
6860 361 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2645 3362 3667 3862 4117 5028 6600 2645
Size: 717 305 195 255 911 1572 3330
Fragments arranged by size:
3330 1572 911 717 305 255 195
ApaI G_GGCC'C
Cuts at: 2237 2787 3255 2237
Size: 550 468 6267
Fragments arranged by size:
6267 550 468
ApaLI G'TGCA_C
Cuts at: 5342 6498 5342
Size: 1156 6129
ApoI r'AATT_y
Cuts at: 1493 4594 4820 4972 7081 1493
Size: 3101 226 152 2109 1697
Fragments arranged by size:
3101 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5839 160
Size: 872 4807 1606
Fragments arranged by size:
4807 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 2230 2323 2332 2431 2596 2625
Size: 73 207 80 93 9 99 165 29
Cuts at: 2625 2786 2852 3647 3873 4215 4610 1870
Size: 161 66 795 226 342 395 4545
Fragments arranged by size:
4545 795 395 342 226 207 165 161
99 93 80 73 66 29 9
BamHI G'GATC_C
Cuts at: 4615 4982 4615
Size: 367 6918
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4065 4606 6061 618
Size: 325 217 627 2278 541 1455 1842
Fragments arranged by size:
2278 1842 1455 627 541 325 217
BanII G_rGCy'C
Cuts at: 1391 2237 2511 2632 2787 2793 3255 3942 4604
Size: 846 274 121 155 6 462 687 662
Cuts at: 4604 1391
Size: 4072
Fragments arranged by size:
4072 846 687 662 462 274 155 121
6
BbeI G_GCGC'C
Cuts at: 4069 4069
Size: 7285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5476 5510 1903
Size: 34 3539 34 3678
Fragments arranged by size:
3678 3539 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3099 5177 6387 3099
Size: 2078 1210 3997
Fragments arranged by size:
3997 2078 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2435 2598
Size: 107 122 71 631 605 388 375 163
Cuts at: 2598 3163 3631 3931 4220 4400 4421 5896 136
Size: 565 468 300 289 180 21 1475 1525
Fragments arranged by size:
1525 1475 631 605 565 468 388 375
300 289 180 163 122 107 71 21
BglII A'GATC_T
Cuts at: 7280 7280
Size: 7285
BlpI GC'TnA_GC
Cuts at: 2588 4702 2588
Size: 2114 5171
Bme1580I G_kGCm'C
Cuts at: 1790 2237 2787 3255 3605 4108 5346 6502 1790
Size: 447 550 468 350 503 1238 1156 2573
Fragments arranged by size:
2573 1238 1156 550 503 468 447 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2209 3447 5016
Size: 416 53 83 399 24 792 1238 1569
Cuts at: 5016 5974 442
Size: 958 1753
Fragments arranged by size:
1753 1569 1238 958 792 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7285
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 4267 2779
Size: 1488 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2749 3552 3822 4100 4139 4530 5945 1239
Size: 1510 803 270 278 39 391 1415 2579
Fragments arranged by size:
2579 1510 1415 803 391 278 270 39
Bpu10I CC'TnA_GC
Cuts at: 3298 3298
Size: 7285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5410 6275 6573 5410
Size: 865 298 6122
Fragments arranged by size:
6122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3137 3142 5948 882
Size: 617 1638 5 2806 2219
Fragments arranged by size:
2806 2219 1638 617 5
BsaAI yAC'GTr
Cuts at: 493 1204 2209 4318 4582 5076 493
Size: 711 1005 2109 264 494 2702
Fragments arranged by size:
2702 2109 1005 711 494 264
BsaBI GATnn'nnATC
Cuts at: 7279 7279
Size: 7285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2489 4066 4296 4476
Size: 53 83 186 936 956 1577 230 180
Cuts at: 4476 5092 5474 6109 275
Size: 616 382 635 1451
Fragments arranged by size:
1577 1451 956 936 635 616 382 230
186 180 83 53
BsaWI w'CCGG_w
Cuts at: 4366 5718 6116 6390 6537 6948 4366
Size: 1352 398 274 147 411 4703
Fragments arranged by size:
4703 1352 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2477 2507 2561
Size: 30 165 30 636 30 408 30 54
Cuts at: 2561 2591 2870 2900 4257 4287 4528 4558 1178
Size: 30 279 30 1357 30 241 30 3905
Fragments arranged by size:
3905 1357 636 408 279 241 165 54
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3509 3665 3821 3861 3893 4304 4388 1186
Size: 2323 156 156 40 32 411 84 4083
Fragments arranged by size:
4083 2323 411 156 156 84 40 32
BseYI C'CCAG_C
Cuts at: 2417 2548 3256 3532 3708 3713 3855 4177 4425
Size: 131 708 276 176 5 142 322 248
Cuts at: 4425 4514 6488 6984 2417
Size: 89 1974 496 2718
Fragments arranged by size:
2718 1974 708 496 322 276 248 176
142 131 89 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3930 1163
Size: 573 2194 4518
Fragments arranged by size:
4518 2194 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2382 2653 4735 5496 6524
Size: 275 186 49 332 271 2082 761 1028
Cuts at: 6524 1540
Size: 2301
Fragments arranged by size:
2301 2082 1028 761 332 275 271 186
49
BsiHKAI G_wGCw'C
Cuts at: 2481 2511 4317 4363 5346 5431 6502 2481
Size: 30 1806 46 983 85 1071 3264
Fragments arranged by size:
3264 1806 1071 983 85 46 30
BsmI GAATG_Cn'
Cuts at: 2750 4796 4889 2750
Size: 2046 93 5146
Fragments arranged by size:
5146 2046 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2480 2849 4515 2480
Size: 369 1666 5250
Fragments arranged by size:
5250 1666 369
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2481 2511 2632 2787 2793 3255
Size: 399 447 244 30 121 155 6 462
Cuts at: 3255 3605 3942 4108 4317 4363 4604 5346 5431
Size: 350 337 166 209 46 241 742 85
Cuts at: 5431 6502 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 462 447 399 350 337
244 241 209 166 155 121 85 46
30 6
BspEI T'CCGG_A
Cuts at: 6948 6948
Size: 7285
BspHI T'CATG_A
Cuts at: 5174 6904 5174
Size: 1730 5555
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3155 4640 844
Size: 2311 1485 3489
Fragments arranged by size:
3489 2311 1485
BsrBI CCG'CTC
Cuts at: 1394 1823 3343 4033 4123 4333 5172 1394
Size: 429 1520 690 90 210 839 3507
Fragments arranged by size:
3507 1520 839 690 429 210 90
BsrDI GCAATG_nn'
Cuts at: 65 1248 3565 3783 5780 5954 65
Size: 1183 2317 218 1997 174 1396
Fragments arranged by size:
2317 1997 1396 1183 218 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2425 2614 2648 2734 3865 4131 4366
Size: 110 615 189 34 86 1131 266 235
Cuts at: 4366 5929 6116 1700
Size: 1563 187 2869
Fragments arranged by size:
2869 1563 1131 615 266 235 189 187
110 86 34
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7285
BssHII G'CGCG_C
Cuts at: 1925 2104 2463 2618 4193 1925
Size: 179 359 155 1575 5017
Fragments arranged by size:
5017 1575 359 179 155
BssSI C'ACGA_G
Cuts at: 2834 5345 6357 2834
Size: 2511 1012 3762
Fragments arranged by size:
3762 2511 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7285
BstBI TT'CG_AA
Cuts at: 2007 6089 2007
Size: 4082 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7285
BstXI CCAn_nnnn'nTGG
Cuts at: 2881 3287 3311 3929 2881
Size: 406 24 618 6237
Fragments arranged by size:
6237 618 406 24
BstYI r'GATC_y
Cuts at: 1972 3679 4615 4982 5367 5384 6836 6922 6934
Size: 1707 936 367 385 17 1452 86 12
Cuts at: 6934 7280 1972
Size: 346 1977
Fragments arranged by size:
1977 1707 1452 936 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4110 4110
Size: 7285
BtgI C'CryG_G
Cuts at: 513 1065 2022 2270 2283 2394 513
Size: 552 957 248 13 111 5404
Fragments arranged by size:
5404 957 552 248 111 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6546
Fragments arranged by size:
6546 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 4095 4801 5595 5615 2305
Size: 1790 706 794 20 3975
Fragments arranged by size:
3975 1790 794 706 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7250
DraI TTT'AAA
Cuts at: 4589 4589
Size: 7285
DraIII CAC_nnn'GTG
Cuts at: 3913 3913
Size: 7285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6292 809
Size: 507 4976 1802
Fragments arranged by size:
4976 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 2650 3313
Size: 54 1425 50 275 18 549 271 663
Cuts at: 3313 3607 3669 3984 4140 4495 4732 5621 7250
Size: 294 62 315 156 355 237 889 1629
Cuts at: 7250 8
Size: 43
Fragments arranged by size:
1629 1425 889 663 549 355 315 294
275 271 237 156 62 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2379 2650 4732 1537
Size: 275 567 271 2082 4090
Fragments arranged by size:
4090 2082 567 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 3192 4572 5215 1353
Size: 1839 1380 643 3423
Fragments arranged by size:
3423 1839 1380 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2402 2569 4442 5872 6246 6392
Size: 1064 138 813 167 1873 1430 374 146
Cuts at: 6392 387
Size: 1280
Fragments arranged by size:
1873 1430 1280 1064 813 374 167 146
138
EcoICRI GAG'CTC
Cuts at: 2509 2509
Size: 7285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2749 2899 2928 3552 3822 3992
Size: 393 809 308 150 29 624 270 170
Cuts at: 3992 4100 4139 4328 4445 4530 5348 5945 6732
Size: 108 39 189 117 85 818 597 787
Cuts at: 6732 7000 1239
Size: 268 1524
Fragments arranged by size:
1524 818 809 787 624 597 393 308
270 268 189 170 150 117 108 85
39 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2753 3197 4460 2753
Size: 444 1263 5578
Fragments arranged by size:
5578 1263 444
EcoO109I rG'GnC_Cy
Cuts at: 2029 2036 2234 2320 2328 2657 2892 3202 3251
Size: 7 198 86 8 329 235 310 49
Cuts at: 3251 3252 3572 3834 4012 4536 2029
Size: 1 320 262 178 524 4778
Fragments arranged by size:
4778 524 329 320 310 262 235 198
178 86 49 8 7 1
EcoRI G'AATT_C
Cuts at: 4594 4972 4594
Size: 378 6907
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 7285
FspI TGC'GCA
Cuts at: 5791 5791
Size: 7285
HaeII r_GCGC'y
Cuts at: 1678 2816 2943 4069 6432 1678
Size: 1138 127 1126 2363 2531
Fragments arranged by size:
2531 2363 1138 1126 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2876 2908 3475
Size: 32 135 32 467 32 805 32 567
Cuts at: 3475 3507 3818 3850 4257 4289 4528 4560 5926
Size: 32 311 32 407 32 239 32 1366
Cuts at: 5926 5958 6000 6032 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1366 805 567 467 407 311 239
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4629 4875 669
Size: 988 281 2691 246 3079
Fragments arranged by size:
3079 2691 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7285
HpaI GTT'AAC
Cuts at: 4875 4875
Size: 7285
KasI G'GCGC_C
Cuts at: 4065 4065
Size: 7285
KpnI G_GTAC'C
Cuts at: 4610 4610
Size: 7285
MfeI C'AATT_G
Cuts at: 4884 4884
Size: 7285
MluI A'CGCG_T
Cuts at: 7161 7161
Size: 7285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2821 2909 2915 6399 6583 1305
Size: 1516 88 6 3484 184 2007
Fragments arranged by size:
3484 2007 1516 184 88 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3315 3609 3671 4497 10
Size: 54 1425 343 1483 294 62 826 2798
Fragments arranged by size:
2798 1483 1425 826 343 294 62 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4054 5243 5602 5761 518
Size: 648 2888 1189 359 159 2042
Fragments arranged by size:
2888 2042 1189 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2285 2362 2396 2452 2470 2677 3503
Size: 666 13 77 34 56 18 207 826
Cuts at: 3503 3536 4282 4701 5378 6526 6771 1606
Size: 33 746 419 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 746 677 666 419 245
207 77 56 34 33 18 13
NaeI GCC'GGC
Cuts at: 2427 2616 2650 2736 3867 2427
Size: 189 34 86 1131 5845
Fragments arranged by size:
5845 1131 189 86 34
NarI GG'CG_CC
Cuts at: 4066 4066
Size: 7285
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5776
Fragments arranged by size:
5776 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7285
NgoMIV G'CCGG_C
Cuts at: 2425 2614 2648 2734 3865 2425
Size: 189 34 86 1131 5845
Fragments arranged by size:
5845 1131 189 86 34
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7285
NotI GC'GGCC_GC
Cuts at: 4732 4732
Size: 7285
NspI r_CATG'y
Cuts at: 1132 1229 4643 6188 1132
Size: 97 3414 1545 2229
Fragments arranged by size:
3414 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2032 2780 3247 3730 4532 1348
Size: 684 748 467 483 802 4101
Fragments arranged by size:
4101 802 748 684 483 467
PciI A'CATG_T
Cuts at: 6184 6184
Size: 7285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7285
PfoI T'CCnGG_A
Cuts at: 2481 3355 2481
Size: 874 6411
PmeI GTTT'AAAC
Cuts at: 4589 4589
Size: 7285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2477 2509 2870 2902 5354 5386 6867 6899 2477
Size: 32 361 32 2452 32 1481 32 2863
Fragments arranged by size:
2863 2452 1481 361 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2320 2328 2657 2892 3572 3834 4012 4536
Size: 284 8 329 235 680 262 178 524
Cuts at: 4536 2036
Size: 4785
Fragments arranged by size:
4785 680 524 329 284 262 235 178
8
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7285
PsiI TTA'TAA
Cuts at: 4855 4855
Size: 7285
PspOMI G'GGCC_C
Cuts at: 2233 2783 3251 2233
Size: 550 468 6267
Fragments arranged by size:
6267 550 468
PspXI vC'TCGA_Gb
Cuts at: 2431 3647 2431
Size: 1216 6069
PstI C_TGCA'G
Cuts at: 830 3097 3367 3508 4041 4090 4263 4281 4637
Size: 2267 270 141 533 49 173 18 356
Cuts at: 4637 830
Size: 3478
Fragments arranged by size:
3478 2267 533 356 270 173 141 49
18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7285
PvuII CAG'CTG
Cuts at: 3503 3536 3503
Size: 33 7252
SacI G_AGCT'C
Cuts at: 2511 2511
Size: 7285
SacII CC_GC'GG
Cuts at: 2273 2286 2397 2273
Size: 13 111 7161
Fragments arranged by size:
7161 111 13
SalI G'TCGA_C
Cuts at: 1936 4627 1936
Size: 2691 4594
SanDI GG'GwC_CC
Cuts at: 2328 2657 2328
Size: 329 6956
SbfI CC_TGCA'GG
Cuts at: 4637 4637
Size: 7285
ScaI AGT'ACT
Cuts at: 3820 5533 7245 3820
Size: 1713 1712 3860
Fragments arranged by size:
3860 1713 1712
SfcI C'TryA_G
Cuts at: 826 1045 2119 3093 3363 3402 3504 4037 4086
Size: 219 1074 974 270 39 102 533 49
Cuts at: 4086 4259 4277 4633 5768 6449 6640 826
Size: 173 18 356 1135 681 191 1471
Fragments arranged by size:
1471 1135 1074 974 681 533 356 270
219 191 173 102 49 39 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2435 3631 4220 2435
Size: 1196 589 5500
Fragments arranged by size:
5500 1196 589
SfoI GGC'GCC
Cuts at: 4067 4067
Size: 7285
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7285
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7285
SmaI CCC'GGG
Cuts at: 1872 2232 2325 2334 2598 2627 4217 4612 1872
Size: 360 93 9 264 29 1590 395 4545
Fragments arranged by size:
4545 1590 395 360 264 93 29 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2431 3647 5389 6290 6552 820
Size: 197 926 488 1216 1742 901 262 1553
Fragments arranged by size:
1742 1553 1216 926 901 488 262 197
SnaBI TAC'GTA
Cuts at: 493 4582 5076 493
Size: 4089 494 2702
Fragments arranged by size:
4089 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7285
SphI G_CATG'C
Cuts at: 1132 4643 1132
Size: 3511 3774
SrfI GCCC'GGGC
Cuts at: 2232 4217 2232
Size: 1985 5300
SspI AAT'ATT
Cuts at: 5 52 5209 5
Size: 47 5157 2081
Fragments arranged by size:
5157 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 4100 4250 1517
Size: 1242 1341 150 4552
Fragments arranged by size:
4552 1341 1242 150
StyI C'CwwG_G
Cuts at: 513 1065 2022 2793 2866 3010 3543 4299 4492
Size: 552 957 771 73 144 533 756 193
Cuts at: 4492 4724 513
Size: 232 3074
Fragments arranged by size:
3074 957 771 756 552 533 232 193
144 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2741 3869 3920 5312 5329 5482 5667
Size: 1255 1161 1128 51 1392 17 153 185
Cuts at: 5667 6112 325
Size: 445 1498
Fragments arranged by size:
1498 1392 1255 1161 1128 445 185 153
51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3818 5531 7243
Size: 274 80 33 51 1443 1841 1713 1712
Cuts at: 7243 96
Size: 138
Fragments arranged by size:
1841 1713 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2561 2593 7245 7277 2561
Size: 32 4652 32 2569
Fragments arranged by size:
4652 2569 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7285
XbaI T'CTAG_A
Cuts at: 4621 4621
Size: 7285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2077 3434 2029
Size: 48 1357 5880
Fragments arranged by size:
5880 1357 48
XhoI C'TCGA_G
Cuts at: 1943 2431 3647 1943
Size: 488 1216 5581
Fragments arranged by size:
5581 1216 488
XmaI C'CCGG_G
Cuts at: 1870 2230 2323 2332 2596 2625 4215 4610 1870
Size: 360 93 9 264 29 1590 395 4545
Fragments arranged by size:
4545 1590 395 360 264 93 29 9
XmnI GAAnn'nnTTC
Cuts at: 5414 5414
Size: 7285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2490 5093 276
Size: 53 83 186 936 956 2603 2468
Fragments arranged by size:
2603 2468 956 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SapI SexAI SwaI