Restriction map for
pFN21ASDA1959
(Circular) (Six-base) MAPSORT of: pFN21ASDA1959.seq Check: 9182 from: 1 to: 6676
pFN21ASDA1959 6676 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2730 4486 278
Size: 53 83 186 936 1194 1756 2468
Fragments arranged by size:
2468 1756 1194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 1937
Size: 2082 4594
Acc65I G'GTAC_C
Cuts at: 3997 3997
Size: 6676
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 4739 6123 6391 1632
Size: 2135 972 1384 268 1917
Fragments arranged by size:
2135 1917 1384 972 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 2656 5575 6552 1203
Size: 1453 2919 977 1327
Fragments arranged by size:
2919 1453 1327 977
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 5405 5405
Size: 6676
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 2202
Size: 32 6644
AlwNI CAG_nnn'CTG
Cuts at: 2481 2609 2988 3139 3790 4419 5991 2481
Size: 128 379 151 651 629 1572 3166
Fragments arranged by size:
3166 1572 651 629 379 151 128
ApaLI G'TGCA_C
Cuts at: 4733 5889 4733
Size: 1156 5520
ApoI r'AATT_y
Cuts at: 1493 3985 4211 4363 6472 1493
Size: 2492 226 152 2109 1697
Fragments arranged by size:
2492 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 2766 2852 3092 4001 1870
Size: 73 340 483 86 240 909 4545
Fragments arranged by size:
4545 909 483 340 240 86 73
BamHI G'GATC_C
Cuts at: 4006 4373 4006
Size: 367 6309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 3997 5452 618
Size: 325 217 627 333 1877 1455 1842
Fragments arranged by size:
1877 1842 1455 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2179 2520 3093 3513 3995 1391
Size: 788 341 573 420 482 4072
Fragments arranged by size:
4072 788 573 482 420 341
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 928
Size: 971 666 5039
Fragments arranged by size:
5039 971 666
BbvCI CC'TCA_GC
Cuts at: 3140 3300 3140
Size: 160 6516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3317 3351 4867 4901 1903
Size: 34 1380 34 1516 34 3678
Fragments arranged by size:
3678 1516 1380 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3558 4568 5778 3558
Size: 1010 1210 4456
Fragments arranged by size:
4456 1210 1010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6676
BfrBI ATG'CAT
Cuts at: 3360 3360
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3159 5287 136
Size: 107 122 71 631 605 1487 2128 1525
Fragments arranged by size:
2128 1525 1487 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2361 6671 2361
Size: 4310 2366
BlpI GC'TnA_GC
Cuts at: 4093 4093
Size: 6676
Bme1580I G_kGCm'C
Cuts at: 1790 4737 5893 1790
Size: 2947 1156 2573
Fragments arranged by size:
2947 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2149 2919 1318
Size: 831 770 5075
Fragments arranged by size:
5075 831 770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2037 2755 3232
Size: 416 53 83 399 24 620 718 477
Cuts at: 3232 3942 4407 5365 442
Size: 710 465 958 1753
Fragments arranged by size:
1753 958 718 710 620 477 465 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 2861 3967 5336 1239
Size: 1295 327 1106 1369 2579
Fragments arranged by size:
2579 1369 1295 1106 327
Bpu10I CC'TnA_GC
Cuts at: 2320 3140 3300 2320
Size: 820 160 5696
Fragments arranged by size:
5696 820 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2561 4801 5666 5964 2561
Size: 2240 865 298 3273
Fragments arranged by size:
3273 2240 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3947 5339 882
Size: 617 2448 1392 2219
Fragments arranged by size:
2448 2219 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 3973 4467 493
Size: 711 2769 494 2702
Fragments arranged by size:
2769 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6670 6670
Size: 6676
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2727 4483 4865 5500
Size: 53 83 186 936 1194 1756 382 635
Cuts at: 5500 275
Size: 1451
Fragments arranged by size:
1756 1451 1194 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5109 5507 5781 5928 6339 5109
Size: 398 274 147 411 5446
Fragments arranged by size:
5446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3298 3328 1178
Size: 30 165 30 1895 30 4526
Fragments arranged by size:
4526 1895 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2084 2144 3096 1186
Size: 898 60 952 4766
Fragments arranged by size:
4766 952 898 60
BseYI C'CCAG_C
Cuts at: 3542 5879 6375 3542
Size: 2337 496 3843
Fragments arranged by size:
3843 2337 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3438 1163
Size: 573 1702 4401
Fragments arranged by size:
4401 1702 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2520 3551 4737 4822 5893 2154
Size: 366 1031 1186 85 1071 2937
Fragments arranged by size:
2937 1186 1071 1031 366 85
BsmI GAATG_Cn'
Cuts at: 2984 4187 4280 2984
Size: 1203 93 5380
Fragments arranged by size:
5380 1203 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3092 3092
Size: 6676
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2179 2520 3093 3513 3551 3995
Size: 399 364 25 341 573 420 38 444
Cuts at: 3995 4737 4822 5893 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 444 420 399 364
341 85 38 25
BspEI T'CCGG_A
Cuts at: 6339 6339
Size: 6676
BspHI T'CATG_A
Cuts at: 4565 6295 4565
Size: 1730 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3565 3782 4031 844
Size: 2721 217 249 3489
Fragments arranged by size:
3489 2721 249 217
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 4563 1394
Size: 429 227 2513 3507
Fragments arranged by size:
3507 2513 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 5171 5345 65
Size: 1183 3923 174 1396
Fragments arranged by size:
3923 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2312 2930 5320 5507 1700
Size: 110 502 618 2390 187 2869
Fragments arranged by size:
2869 2390 618 502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2076 96
Size: 1980 4696
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 4736 5748 4736
Size: 1012 5664
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2609 794
Size: 1815 4861
BstBI TT'CG_AA
Cuts at: 2007 5480 2007
Size: 3473 3203
BstEII G'GTnAC_C
Cuts at: 1184 2255 1184
Size: 1071 5605
BstYI r'GATC_y
Cuts at: 1972 2361 2907 3498 3834 3898 4006 4373 4758
Size: 389 546 591 336 64 108 367 385
Cuts at: 4758 4775 6227 6313 6325 6671 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 591 546 389 385 367 346
336 108 86 64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2160 2202 2469 3922 513
Size: 552 957 138 42 267 1453 3267
Fragments arranged by size:
3267 1453 957 552 267 138 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3187 487
Size: 683 56 1961 3976
Fragments arranged by size:
3976 1961 683 56
BtsI GCAGTG_nn'
Cuts at: 4192 4986 5006 4192
Size: 794 20 5862
Fragments arranged by size:
5862 794 20
ClaI AT'CG_AT
Cuts at: 1554 3411 1554
Size: 1857 4819
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3357 3392 529
Size: 35 2793 35 3813
Fragments arranged by size:
3813 2793 35 35
DraI TTT'AAA
Cuts at: 3980 3980
Size: 6676
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5683 809
Size: 507 4367 1802
Fragments arranged by size:
4367 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 4123 5012
Size: 54 1425 50 275 18 205 2088 889
Cuts at: 5012 6641 8
Size: 1629 43
Fragments arranged by size:
2088 1629 1425 889 275 205 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4606 1353
Size: 3253 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5263 5637 5783 387
Size: 1064 138 3674 374 146 1280
Fragments arranged by size:
3674 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2518 2518
Size: 6676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 2861 3767 3967 4739 5336 6123
Size: 393 902 327 906 200 772 597 787
Cuts at: 6123 6391 1239
Size: 268 1524
Fragments arranged by size:
1524 906 902 787 772 597 393 327
268 200
EcoO109I rG'GnC_Cy
Cuts at: 2572 3787 2572
Size: 1215 5461
EcoRI G'AATT_C
Cuts at: 3985 4363 3985
Size: 378 6298
EcoRV GAT'ATC
Cuts at: 2773 2773
Size: 6676
FspI TGC'GCA
Cuts at: 5182 5182
Size: 6676
HaeII r_GCGC'y
Cuts at: 1678 2116 2144 5823 1678
Size: 438 28 3679 2531
Fragments arranged by size:
3679 2531 438 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 3091 3123 3826
Size: 32 135 32 1025 32 462 32 703
Cuts at: 3826 3858 5317 5349 5391 5423 1373
Size: 32 1459 32 42 32 2626
Fragments arranged by size:
2626 1459 1025 703 462 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3148 4020 4266 669
Size: 988 281 1210 872 246 3079
Fragments arranged by size:
3079 1210 988 872 281 246
HindIII A'AGCT_T
Cuts at: 748 2330 748
Size: 1582 5094
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KpnI G_GTAC'C
Cuts at: 4001 4001
Size: 6676
MfeI C'AATT_G
Cuts at: 4275 4275
Size: 6676
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2361 2556 2885 3057 5790 5974 1305
Size: 1056 195 329 172 2733 184 2007
Fragments arranged by size:
2733 2007 1056 329 195 184 172
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4854
Fragments arranged by size:
4854 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2253 2867 3347 4634 4993 5152 518
Size: 648 1087 614 480 1287 359 159 2042
Fragments arranged by size:
2042 1287 1087 648 614 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 2506 3139 3304 3892 4092 4769 5917 6162
Size: 900 633 165 588 200 677 1148 245
Cuts at: 6162 1606
Size: 2120
Fragments arranged by size:
2120 1148 900 677 633 588 245 200
165
NcoI C'CATG_G
Cuts at: 513 1065 2022 2160 3922 513
Size: 552 957 138 1762 3267
Fragments arranged by size:
3267 1762 957 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NsiI A_TGCA'T
Cuts at: 3362 3362
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 2232 2633 2660 3286 3364 4034 5579
Size: 97 1003 401 27 626 78 670 1545
Cuts at: 5579 1132
Size: 2229
Fragments arranged by size:
2229 1545 1003 670 626 401 97 78
27
PasI CC'CwG_GG
Cuts at: 1348 2449 1348
Size: 1101 5575
PciI A'CATG_T
Cuts at: 2656 5575 2656
Size: 2919 3757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6676
PfoI T'CCnGG_A
Cuts at: 2265 2265
Size: 6676
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 4745 4777 6258 6290 2011
Size: 32 2702 32 1481 32 2397
Fragments arranged by size:
2702 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 2572
Size: 6676
PshAI GACnn'nnGTC
Cuts at: 1613 2471 1613
Size: 858 5818
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PstI C_TGCA'G
Cuts at: 830 2466 2605 3589 4028 830
Size: 1636 139 984 439 3478
Fragments arranged by size:
3478 1636 984 439 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
PvuII CAG'CTG
Cuts at: 3892 3892
Size: 6676
SacI G_AGCT'C
Cuts at: 2520 2520
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 4018 1936
Size: 2082 4594
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 3227 4924 6636 3227
Size: 1697 1712 3267
Fragments arranged by size:
3267 1712 1697
SfcI C'TryA_G
Cuts at: 826 1045 2462 2601 3585 3613 4024 5159 5840
Size: 219 1417 139 984 28 411 1135 681
Cuts at: 5840 6031 826
Size: 191 1471
Fragments arranged by size:
1471 1417 1135 984 681 411 219 191
139 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 2285 2854 4003 1872
Size: 413 569 1149 4545
Fragments arranged by size:
4545 1149 569 413
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 4780 5681 5943 820
Size: 197 926 633 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 633 262 197
SnaBI TAC'GTA
Cuts at: 493 3973 4467 493
Size: 3480 494 2702
Fragments arranged by size:
3480 2702 494
SpeI A'CTAG_T
Cuts at: 152 3373 152
Size: 3221 3455
SphI G_CATG'C
Cuts at: 1132 3364 4034 1132
Size: 2232 670 3774
Fragments arranged by size:
3774 2232 670
SspI AAT'ATT
Cuts at: 5 52 4600 5
Size: 47 4548 2081
Fragments arranged by size:
4548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2022 2160 2193 2967 3807 3922 4115
Size: 552 957 138 33 774 840 115 193
Cuts at: 4115 513
Size: 3074
Fragments arranged by size:
3074 957 840 774 552 193 138 115
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2737 3368 4703 4720 4873 5058 5503
Size: 1255 1157 631 1335 17 153 185 445
Cuts at: 5503 325
Size: 1498
Fragments arranged by size:
1498 1335 1255 1157 631 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 3225 4922
Size: 274 80 33 51 1443 99 1149 1697
Cuts at: 4922 6634 96
Size: 1712 138
Fragments arranged by size:
1712 1697 1443 1149 274 138 99 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 6636 6668 3740
Size: 32 2864 32 3748
Fragments arranged by size:
3748 2864 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6676
XbaI T'CTAG_A
Cuts at: 4012 4012
Size: 6676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3929 3929
Size: 6676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6676
XmaI C'CCGG_G
Cuts at: 1870 2283 2852 4001 1870
Size: 413 569 1149 4545
Fragments arranged by size:
4545 1149 569 413
XmnI GAAnn'nnTTC
Cuts at: 4805 4805
Size: 6676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2728 4484 276
Size: 53 83 186 936 1194 1756 2468
Fragments arranged by size:
2468 1756 1194 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI