Restriction map for
pFN21ASDA1957
(Circular) (Six-base) MAPSORT of: pFN21ASDA1957.seq Check: 6534 from: 1 to: 5992
pFN21ASDA1957 5992 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3006 3006
Size: 5992
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2462 3802 278
Size: 53 83 186 936 926 1340 2468
Fragments arranged by size:
2468 1340 936 926 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2354 2638 4055 5439 5707 1632
Size: 722 284 1417 1384 268 1917
Fragments arranged by size:
1917 1417 1384 722 284 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 2037 4891 5868 1203
Size: 834 2854 977 1327
Fragments arranged by size:
2854 1327 977 834
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 2383 2677 4721 2383
Size: 294 2044 3654
Fragments arranged by size:
3654 2044 294
AleI CACnn'nnGTG
Cuts at: 1166 3107 1166
Size: 1941 4051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2345 1184
Size: 1161 4831
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 2501
Size: 32 5960
AlwNI CAG_nnn'CTG
Cuts at: 2254 3735 5307 2254
Size: 1481 1572 2939
Fragments arranged by size:
2939 1572 1481
ApaI G_GGCC'C
Cuts at: 2130 2201 2234 2612 2632 2936 3168 2130
Size: 71 33 378 20 304 232 4954
Fragments arranged by size:
4954 378 304 232 71 33 20
ApaLI G'TGCA_C
Cuts at: 2993 4049 5205 2993
Size: 1056 1156 3780
Fragments arranged by size:
3780 1156 1056
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2194 2843 2891 2937 3242 3317
Size: 73 142 109 649 48 46 305 75
Cuts at: 3317 1870
Size: 4545
Fragments arranged by size:
4545 649 305 142 109 75 73 48
46
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2443 2568 2853 2872
Size: 325 217 627 636 20 125 285 19
Cuts at: 2872 3073 3313 4768 618
Size: 201 240 1455 1842
Fragments arranged by size:
1842 1455 636 627 325 285 240 217
201 125 20 19
BanII G_rGCy'C
Cuts at: 1391 2130 2201 2234 2291 2492 2539 2555 2612
Size: 739 71 33 57 201 47 16 57
Cuts at: 2612 2632 2642 2936 3168 3311 1391
Size: 20 10 294 232 143 4072
Fragments arranged by size:
4072 739 294 232 201 143 71 57
57 47 33 20 16 10
BbeI G_GCGC'C
Cuts at: 2572 2857 2572
Size: 285 5707
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3124 928
Size: 971 1225 3796
Fragments arranged by size:
3796 1225 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3088 3122 4183 4217 1903
Size: 34 1151 34 1061 34 3678
Fragments arranged by size:
3678 1151 1061 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2264 2309 2480
Size: 107 122 71 631 605 592 45 171
Cuts at: 2480 2802 2940 2978 4603 136
Size: 322 138 38 1625 1525
Fragments arranged by size:
1625 1525 631 605 592 322 171 138
122 107 71 45 38
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2201 2234 2448 2612 2632 2875 2936
Size: 340 71 33 214 164 20 243 61
Cuts at: 2936 2997 3168 4053 5209 1790
Size: 61 171 885 1156 2573
Fragments arranged by size:
2573 1156 885 340 243 214 171 164
71 61 61 33 20
BmgBI CAC'GTC
Cuts at: 1318 2241 1318
Size: 923 5069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4681 442
Size: 416 53 83 399 24 2306 958 1753
Fragments arranged by size:
2306 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4652 1239
Size: 3413 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 4117 4982 5280 3209
Size: 908 865 298 3921
Fragments arranged by size:
3921 908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4655 882
Size: 617 3156 2219
Fragments arranged by size:
3156 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2353 2929 3289 3783 493
Size: 711 1149 576 360 494 2702
Fragments arranged by size:
2702 1149 711 576 494 360
BsaBI GATnn'nnATC
Cuts at: 2360 5986 2360
Size: 3626 2366
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2150 2403 2459 2569
Size: 53 83 186 936 617 253 56 110
Cuts at: 2569 2835 2854 3036 3799 4181 4816 275
Size: 266 19 182 763 382 635 1451
Fragments arranged by size:
1451 936 763 635 617 382 266 253
186 182 110 83 56 53 19
BsaWI w'CCGG_w
Cuts at: 3177 4425 4823 5097 5244 5655 3177
Size: 1248 398 274 147 411 3514
Fragments arranged by size:
3514 1248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2102 2132 2501 2531 1178
Size: 30 165 30 699 30 369 30 4639
Fragments arranged by size:
4639 699 369 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 1186
Size: 1219 4773
BseYI C'CCAG_C
Cuts at: 2227 5195 5691 2227
Size: 2968 496 2528
Fragments arranged by size:
2968 2528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2647 2688 3025 3442 4203 5231
Size: 275 186 646 41 337 417 761 1028
Cuts at: 5231 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 646 417 337 275 186
41
BsiHKAI G_wGCw'C
Cuts at: 2532 2919 2997 4053 4138 5209 2532
Size: 387 78 1056 85 1071 3315
Fragments arranged by size:
3315 1071 1056 387 85 78
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2201 2234 2291 2448 2492 2532
Size: 399 340 71 33 57 157 44 40
Cuts at: 2532 2539 2555 2612 2632 2642 2875 2919 2936
Size: 7 16 57 20 10 233 44 17
Cuts at: 2936 2997 3168 3311 4053 4138 5209 1391
Size: 61 171 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 233 171 157
143 85 71 61 57 57 44 44
40 33 20 17 16 10 7
BspEI T'CCGG_A
Cuts at: 3177 5655 3177
Size: 2478 3514
BspHI T'CATG_A
Cuts at: 2634 3881 5611 2634
Size: 1247 1730 3015
Fragments arranged by size:
3015 1730 1247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3006 3347 844
Size: 2162 341 3489
Fragments arranged by size:
3489 2162 341
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 2724 3879 1394
Size: 429 230 671 1155 3507
Fragments arranged by size:
3507 1155 671 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 4487 4661 65
Size: 1183 898 2341 174 1396
Fragments arranged by size:
2341 1396 1183 898 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2850 2972 4636 4823 1700
Size: 110 1040 122 1664 187 2869
Fragments arranged by size:
2869 1664 1040 187 122 110
BsrGI T'GTAC_A
Cuts at: 96 2133 96
Size: 2037 3955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 2497 4052 5064 2497
Size: 1555 1012 3425
Fragments arranged by size:
3425 1555 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3051 794
Size: 2257 3735
BstBI TT'CG_AA
Cuts at: 2007 4796 2007
Size: 2789 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2909 2909
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2160 3322 3689 4074 4091 5543 5629 5641
Size: 188 1162 367 385 17 1452 86 12
Cuts at: 5641 5987 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1162 385 367 346 188 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3191 3191
Size: 5992
BtgI C'CryG_G
Cuts at: 513 1065 2202 2282 2790 2800 513
Size: 552 1137 80 508 10 3705
Fragments arranged by size:
3705 1137 552 508 80 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 3508 4302 4322 3508
Size: 794 20 5178
Fragments arranged by size:
5178 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DraIII CAC_nnn'GTG
Cuts at: 2791 2791
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2481 2644 2685
Size: 54 1425 50 275 18 651 163 41
Cuts at: 2685 2958 2974 3003 3439 4328 5957 8
Size: 273 16 29 436 889 1629 43
Fragments arranged by size:
1629 1425 889 651 436 275 273 163
54 50 43 41 29 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2644 2685 3439 1537
Size: 275 832 41 754 4090
Fragments arranged by size:
4090 832 754 275 41
EarI CTCTTCn'nnn_
Cuts at: 1353 3922 1353
Size: 2569 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2638 4055 4652 5439 5707 1239
Size: 393 722 284 1417 597 787 268 1524
Fragments arranged by size:
1524 1417 787 722 597 393 284 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 5992
EcoO109I rG'GnC_Cy
Cuts at: 2126 2127 2198 2231 2608 2609 2628 2629 2679
Size: 1 71 33 377 1 19 1 50
Cuts at: 2679 2706 2933 3013 3164 2126
Size: 27 227 80 151 4954
Fragments arranged by size:
4954 377 227 151 80 71 50 33
27 19 1 1 1
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FseI GG_CCGG'CC
Cuts at: 2976 2976
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 2572 2857 5139 1678
Size: 894 285 2282 2531
Fragments arranged by size:
2531 2282 894 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2369 2401 2663
Size: 32 135 32 530 32 235 32 262
Cuts at: 2663 2695 4633 4665 4707 4739 1373
Size: 32 1938 32 42 32 2626
Fragments arranged by size:
2626 1938 530 262 235 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2148 3336 3582 669
Size: 988 281 210 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246 210
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KasI G'GCGC_C
Cuts at: 2568 2853 2568
Size: 285 5707
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 5106 5290 1305
Size: 918 2883 184 2007
Fragments arranged by size:
2883 2007 918 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3107 3950 4309 4468 518
Size: 648 1941 843 359 159 2042
Fragments arranged by size:
2042 1941 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2204 2582 3408 4085 5233 5478 1606
Size: 598 378 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 677 598 378 245
NaeI GCC'GGC
Cuts at: 2852 2974 2852
Size: 122 5870
NarI GG'CG_CC
Cuts at: 2569 2854 2569
Size: 285 5707
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5440
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 2850 2972 2850
Size: 122 5870
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2041 3350 4895 1132
Size: 97 812 1309 1545 2229
Fragments arranged by size:
2229 1545 1309 812 97
PasI CC'CwG_GG
Cuts at: 1348 2419 2558 2613 1348
Size: 1071 139 55 4727
Fragments arranged by size:
4727 1071 139 55
PciI A'CATG_T
Cuts at: 2037 4891 2037
Size: 2854 3138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2040 1108
Size: 932 5060
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PmlI CAC'GTG
Cuts at: 2353 2929 2353
Size: 576 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2243 2275 2501 2533 4061 4093 5574 5606 2243
Size: 32 226 32 1528 32 1481 32 2629
Fragments arranged by size:
2629 1528 1481 226 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2679 2706 2679
Size: 27 5965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 2126 2197 2230 2608 2628 2932 3164 2126
Size: 71 33 378 20 304 232 4954
Fragments arranged by size:
4954 378 304 232 71 33 20
PstI C_TGCA'G
Cuts at: 830 3090 3344 830
Size: 2260 254 3478
Fragments arranged by size:
3478 2260 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2582 2582
Size: 5992
SacII CC_GC'GG
Cuts at: 2205 2205
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SanDI GG'GwC_CC
Cuts at: 2706 2706
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SfcI C'TryA_G
Cuts at: 826 1045 3086 3340 4475 5156 5347 826
Size: 219 2041 254 1135 681 191 1471
Fragments arranged by size:
2041 1471 1135 681 254 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2264 2978 2264
Size: 714 5278
SfoI GGC'GCC
Cuts at: 2570 2855 2570
Size: 285 5707
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 2087 2845 3319 1872
Size: 215 758 474 4545
Fragments arranged by size:
4545 758 474 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 4096 4997 5259 820
Size: 197 926 1281 872 901 262 1553
Fragments arranged by size:
1553 1281 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 3289 3783 493
Size: 2796 494 2702
Fragments arranged by size:
2796 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 2117 1517
Size: 600 5392
StyI C'CwwG_G
Cuts at: 513 1065 2121 2448 2586 2766 3431 513
Size: 552 1056 327 138 180 665 3074
Fragments arranged by size:
3074 1056 665 552 327 180 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4019 4036 4189 4374 4819 325
Size: 1255 2439 17 153 185 445 1498
Fragments arranged by size:
2439 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 4238 5950
Size: 274 80 33 51 1443 156 2105 1712
Cuts at: 5950 96
Size: 138
Fragments arranged by size:
2105 1712 1443 274 156 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5952 5984 5952
Size: 32 5960
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5992
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 2085 2843 3317 1870
Size: 215 758 474 4545
Fragments arranged by size:
4545 758 474 215
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2460 3800 276
Size: 53 83 186 936 926 1340 2468
Fragments arranged by size:
2468 1340 936 926 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SapI
SexAI SrfI SwaI XcmI