Restriction map for
pFN21ASDA1956
(Circular) (Six-base) MAPSORT of: pFN21ASDA1956.seq Check: 4675 from: 1 to: 6757
pFN21ASDA1956 6757 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2234 2234
Size: 6757
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4567 278
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4100 1937
Size: 2163 4594
Acc65I G'GTAC_C
Cuts at: 4078 4078
Size: 6757
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3901 4820 6204 6472 1632
Size: 2269 919 1384 268 1917
Fragments arranged by size:
2269 1917 1384 919 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 2668 5656 6633 1203
Size: 1465 2988 977 1327
Fragments arranged by size:
2988 1465 1327 977
AgeI A'CCGG_T
Cuts at: 5588 5588
Size: 6757
AhdI GACnn_n'nnGTC
Cuts at: 5486 5486
Size: 6757
AleI CACnn'nnGTG
Cuts at: 1166 2271 2355 3117 1166
Size: 1105 84 762 4806
Fragments arranged by size:
4806 1105 762 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6757
AlwNI CAG_nnn'CTG
Cuts at: 2347 4500 6072 2347
Size: 2153 1572 3032
Fragments arranged by size:
3032 2153 1572
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 3740 4066 4292 4444 6553 1493
Size: 2247 326 226 152 2109 1697
Fragments arranged by size:
2247 2109 1697 326 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5311 160
Size: 872 4279 1606
Fragments arranged by size:
4279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 3864 4082 1870
Size: 73 1921 218 4545
Fragments arranged by size:
4545 1921 218 73
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 3170 4078 5533 618
Size: 325 217 627 398 985 908 1455 1842
Fragments arranged by size:
1842 1455 985 908 627 398 325 217
BanII G_rGCy'C
Cuts at: 1391 3674 4076 1391
Size: 2283 402 4072
Fragments arranged by size:
4072 2283 402
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4948 4982 1903
Size: 34 3011 34 3678
Fragments arranged by size:
3678 3011 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4649 5859 4649
Size: 1210 5547
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6757
BfrBI ATG'CAT
Cuts at: 2646 2646
Size: 6757
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5368 136
Size: 107 122 71 631 605 3696 1525
Fragments arranged by size:
3696 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6752 6752
Size: 6757
BlpI GC'TnA_GC
Cuts at: 4174 4174
Size: 6757
Bme1580I G_kGCm'C
Cuts at: 1790 2033 3173 4818 5974 1790
Size: 243 1140 1645 1156 2573
Fragments arranged by size:
2573 1645 1156 1140 243
BmgBI CAC'GTC
Cuts at: 1318 2119 1318
Size: 801 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2143 2577 2790
Size: 416 53 83 399 24 726 434 213
Cuts at: 2790 2874 4488 5446 442
Size: 84 1614 958 1753
Fragments arranged by size:
1753 1614 958 726 434 416 399 213
84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2970 3054 3306 3390 3474 3558 3642
Size: 908 823 84 252 84 84 84 84
Cuts at: 3642 3733 3801 3969 5417 1239
Size: 91 68 168 1448 2579
Fragments arranged by size:
2579 1448 908 823 252 168 91 84
84 84 84 84 68
Bpu10I CC'TnA_GC
Cuts at: 2536 2536
Size: 6757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4882 5747 6045 4882
Size: 865 298 5594
Fragments arranged by size:
5594 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5420 882
Size: 617 3921 2219
Fragments arranged by size:
3921 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2671 4054 4548 493
Size: 711 1467 1383 494 2702
Fragments arranged by size:
2702 1467 1383 711 494
BsaBI GATnn'nnATC
Cuts at: 6751 6751
Size: 6757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4564 4946 5581 275
Size: 53 83 186 936 3031 382 635 1451
Fragments arranged by size:
3031 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5190 5588 5862 6009 6420 5190
Size: 398 274 147 411 5527
Fragments arranged by size:
5527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6532
Fragments arranged by size:
6532 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2195 2504 1186
Size: 901 108 309 5439
Fragments arranged by size:
5439 901 309 108
BseYI C'CCAG_C
Cuts at: 2531 2718 5960 6456 2531
Size: 187 3242 496 2832
Fragments arranged by size:
3242 2832 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2333 3745 3904 3988 1163
Size: 573 597 1412 159 84 3932
Fragments arranged by size:
3932 1412 597 573 159 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4207 4968 5996 1540
Size: 275 186 2206 761 1028 2301
Fragments arranged by size:
2301 2206 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3674 4818 4903 5974 3674
Size: 1144 85 1071 4457
Fragments arranged by size:
4457 1144 1071 85
BsmI GAATG_Cn'
Cuts at: 2648 3803 4268 4361 2648
Size: 1155 465 93 5044
Fragments arranged by size:
5044 1155 465 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 3173 3674 4076 4818 4903 5974
Size: 399 243 1140 501 402 742 85 1071
Cuts at: 5974 1391
Size: 2174
Fragments arranged by size:
2174 1140 1071 742 501 402 399 243
85
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 4646 6376 4646
Size: 1730 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 4112 844
Size: 1390 1878 3489
Fragments arranged by size:
3489 1878 1390
BsrBI CCG'CTC
Cuts at: 1394 1823 4644 1394
Size: 429 2821 3507
Fragments arranged by size:
3507 2821 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5252 5426 65
Size: 1183 4004 174 1396
Fragments arranged by size:
4004 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5401 5588 1700
Size: 110 3591 187 2869
Fragments arranged by size:
3591 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6757
BssSI C'ACGA_G
Cuts at: 4817 5829 4817
Size: 1012 5745
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2347 794
Size: 1553 5204
BstBI TT'CG_AA
Cuts at: 2007 5561 2007
Size: 3554 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6757
BstYI r'GATC_y
Cuts at: 1972 4087 4454 4839 4856 6308 6394 6406 6752
Size: 2115 367 385 17 1452 86 12 346
Cuts at: 6752 1972
Size: 1977
Fragments arranged by size:
2115 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3260 3260
Size: 6757
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6018
Fragments arranged by size:
6018 683 56
BtsI GCAGTG_nn'
Cuts at: 2446 3702 3976 4273 5067 5087 2446
Size: 1256 274 297 794 20 4116
Fragments arranged by size:
4116 1256 794 297 274 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6722
DraI TTT'AAA
Cuts at: 3844 4061 3844
Size: 217 6540
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5764 809
Size: 507 4448 1802
Fragments arranged by size:
4448 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4204 5093 6722
Size: 54 1425 50 275 18 2374 889 1629
Cuts at: 6722 8
Size: 43
Fragments arranged by size:
2374 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 2459 3679 4006 4687 1353
Size: 839 267 1220 327 681 3423
Fragments arranged by size:
3423 1220 839 681 327 267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5344 5718 5864 387
Size: 1064 138 3755 374 146 1280
Fragments arranged by size:
3755 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3672 3672
Size: 6757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2970 3054 3306 3390 3474 3558
Size: 393 515 823 84 252 84 84 84
Cuts at: 3558 3642 3733 3801 3901 3969 4820 5417 6204
Size: 84 91 68 100 68 851 597 787
Cuts at: 6204 6472 1239
Size: 268 1524
Fragments arranged by size:
1524 851 823 787 597 515 393 268
252 100 91 84 84 84 84 84
68 68
EcoNI CCTnn'n_nnAGG
Cuts at: 2090 2241 2090
Size: 151 6606
EcoRI G'AATT_C
Cuts at: 4066 4444 4066
Size: 378 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4059 4059
Size: 6757
FspI TGC'GCA
Cuts at: 3837 5263 3837
Size: 1426 5331
HaeII r_GCGC'y
Cuts at: 1678 5904 1678
Size: 4226 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2480 2512 2585 2617 5398
Size: 32 135 32 908 32 73 32 2781
Cuts at: 5398 5430 5472 5504 1373
Size: 32 42 32 2626
Fragments arranged by size:
2781 2626 908 135 73 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4101 4347 669
Size: 988 281 2163 246 3079
Fragments arranged by size:
3079 2163 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6757
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KpnI G_GTAC'C
Cuts at: 4082 4082
Size: 6757
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5871 6055 1305
Size: 4566 184 2007
Fragments arranged by size:
4566 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4935
Fragments arranged by size:
4935 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 2355 3117 3469 4715 5074 5233
Size: 648 1105 84 762 352 1246 359 159
Cuts at: 5233 518
Size: 2042
Fragments arranged by size:
2042 1246 1105 762 648 359 352 159
84
MspA1I CmG'CkG
Cuts at: 1606 2634 4173 4850 5998 6243 1606
Size: 1028 1539 677 1148 245 2120
Fragments arranged by size:
2120 1539 1148 1028 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6205
NdeI CA'TA_TG
Cuts at: 387 3466 387
Size: 3079 3678
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6757
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NsiI A_TGCA'T
Cuts at: 2648 2648
Size: 6757
NspI r_CATG'y
Cuts at: 1132 1229 4115 5660 1132
Size: 97 2886 1545 2229
Fragments arranged by size:
2886 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2152 1348
Size: 804 5953
PciI A'CATG_T
Cuts at: 5656 5656
Size: 6757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6757
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4826 4858 6339 6371 4826
Size: 32 1481 32 5212
Fragments arranged by size:
5212 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6757
PsiI TTA'TAA
Cuts at: 4327 4327
Size: 6757
PspXI vC'TCGA_Gb
Cuts at: 3864 3864
Size: 6757
PstI C_TGCA'G
Cuts at: 830 2553 3709 4109 830
Size: 1723 1156 400 3478
Fragments arranged by size:
3478 1723 1156 400
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6757
PvuII CAG'CTG
Cuts at: 2634 2634
Size: 6757
SacI G_AGCT'C
Cuts at: 3674 3674
Size: 6757
SalI G'TCGA_C
Cuts at: 1936 4099 1936
Size: 2163 4594
SapI GCTCTTCn'nnn_
Cuts at: 2192 3679 4006 2192
Size: 1487 327 4943
Fragments arranged by size:
4943 1487 327
SbfI CC_TGCA'GG
Cuts at: 4109 4109
Size: 6757
ScaI AGT'ACT
Cuts at: 5005 6717 5005
Size: 1712 5045
SfcI C'TryA_G
Cuts at: 826 1045 2431 2549 3705 4105 5240 5921 6112
Size: 219 1386 118 1156 400 1135 681 191
Cuts at: 6112 826
Size: 1471
Fragments arranged by size:
1471 1386 1156 1135 681 400 219 191
118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6757
SmaI CCC'GGG
Cuts at: 1872 4084 1872
Size: 2212 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3864 4861 5762 6024 820
Size: 197 926 1921 997 901 262 1553
Fragments arranged by size:
1921 1553 997 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4054 4548 493
Size: 3561 494 2702
Fragments arranged by size:
3561 2702 494
SpeI A'CTAG_T
Cuts at: 152 3116 152
Size: 2964 3793
SphI G_CATG'C
Cuts at: 1132 4115 1132
Size: 2983 3774
SspI AAT'ATT
Cuts at: 5 52 4681 5
Size: 47 4629 2081
Fragments arranged by size:
4629 2081 47
StuI AGG'CCT
Cuts at: 1517 3379 3631 3790 3958 1517
Size: 1862 252 159 168 4316
Fragments arranged by size:
4316 1862 252 168 159
StyI C'CwwG_G
Cuts at: 513 1065 2416 4196 513
Size: 552 1351 1780 3074
Fragments arranged by size:
3074 1780 1351 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4784 4801 4954 5139 5584 325
Size: 1255 3204 17 153 185 445 1498
Fragments arranged by size:
3204 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3940 5003 6715
Size: 274 80 33 51 1443 1963 1063 1712
Cuts at: 6715 96
Size: 138
Fragments arranged by size:
1963 1712 1443 1063 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6717 6749 6717
Size: 32 6725
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 2699 4093 2699
Size: 1394 5363
XhoI C'TCGA_G
Cuts at: 1943 3864 1943
Size: 1921 4836
XmaI C'CCGG_G
Cuts at: 1870 4082 1870
Size: 2212 4545
XmnI GAAnn'nnTTC
Cuts at: 3743 4000 4886 3743
Size: 257 886 5614
Fragments arranged by size:
5614 886 257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4565 276
Size: 53 83 186 936 3031 2468
Fragments arranged by size:
3031 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI