Restriction map for
pFN21ASDA1932
(Circular) (Six-base) MAPSORT of: pFN21ASDA1932.seq Check: 8646 from: 1 to: 7066
pFN21ASDA1932 7066 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3578 3578
Size: 7066
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4876 278
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4409 1937
Size: 2472 4594
Acc65I G'GTAC_C
Cuts at: 2289 4387 2289
Size: 2098 4968
AclI AA'CG_TT
Cuts at: 1563 5193 5566 1563
Size: 3630 373 3063
Fragments arranged by size:
3630 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3452 4001 5129 6513 6781 1632
Size: 1820 549 1128 1384 268 1917
Fragments arranged by size:
1917 1820 1384 1128 549 268
AfeI AGC'GCT
Cuts at: 2807 2807
Size: 7066
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6869
AflIII A'CryG_T
Cuts at: 1203 2238 3699 5965 6942 1203
Size: 1035 1461 2266 977 1327
Fragments arranged by size:
2266 1461 1327 1035 977
AgeI A'CCGG_T
Cuts at: 5897 5897
Size: 7066
AhdI GACnn_n'nnGTC
Cuts at: 2607 5795 2607
Size: 3188 3878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4009 1184
Size: 2825 4241
AlwNI CAG_nnn'CTG
Cuts at: 2158 2183 2392 3553 3739 4809 6381 2158
Size: 25 209 1161 186 1070 1572 2843
Fragments arranged by size:
2843 1572 1161 1070 209 186 25
ApaI G_GGCC'C
Cuts at: 2505 3316 4033 2505
Size: 811 717 5538
Fragments arranged by size:
5538 811 717
ApaLI G'TGCA_C
Cuts at: 2229 5123 6279 2229
Size: 2894 1156 3016
Fragments arranged by size:
3016 2894 1156
ApoI r'AATT_y
Cuts at: 1493 4375 4601 4753 6862 1493
Size: 2882 226 152 2109 1697
Fragments arranged by size:
2882 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5620 160
Size: 872 4588 1606
Fragments arranged by size:
4588 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 2943 3065 3090 3488 3937 4391
Size: 73 802 198 122 25 398 449 454
Cuts at: 4391 1870
Size: 4545
Fragments arranged by size:
4545 802 454 449 398 198 122 73
25
BamHI G'GATC_C
Cuts at: 4396 4763 4396
Size: 367 6699
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2289 3041 3077 3367 3566
Size: 325 217 627 502 752 36 290 199
Cuts at: 3566 4159 4387 5842 618
Size: 593 228 1455 1842
Fragments arranged by size:
1842 1455 752 627 593 502 325 290
228 217 199 36
BanII G_rGCy'C
Cuts at: 1391 2440 2505 2928 2966 3091 3316 3495 4033
Size: 1049 65 423 38 125 225 179 538
Cuts at: 4033 4385 1391
Size: 352 4072
Fragments arranged by size:
4072 1049 538 423 352 225 179 125
65 38
BbeI G_GCGC'C
Cuts at: 3371 3371
Size: 7066
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 3121 928
Size: 971 156 1066 4873
Fragments arranged by size:
4873 1066 971 156
BbvCI CC'TCA_GC
Cuts at: 2257 2385 2412 2460 2257
Size: 128 27 48 6863
Fragments arranged by size:
6863 128 48 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3773 3807 3881 3915 5257 5291 1903
Size: 34 1836 34 74 34 1342 34 3678
Fragments arranged by size:
3678 1836 1342 74 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4958 6168 4958
Size: 1210 5856
BclI T'GATC_A
Cuts at: 1548 2041 4143 1548
Size: 493 2102 4471
Fragments arranged by size:
4471 2102 493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 4136 5677
Size: 107 122 71 631 605 481 1983 1541
Cuts at: 5677 136
Size: 1525
Fragments arranged by size:
1983 1541 1525 631 605 481 122 107
71
BglII A'GATC_T
Cuts at: 7061 7061
Size: 7066
BlpI GC'TnA_GC
Cuts at: 2403 4483 2403
Size: 2080 4986
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2505 3046 3316 3569 3613 4033 5127
Size: 443 272 541 270 253 44 420 1094
Cuts at: 5127 6283 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1094 541 443 420 272 270
253 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2336 4247 4797
Size: 416 53 83 399 24 919 1911 550
Cuts at: 4797 5755 442
Size: 958 1753
Fragments arranged by size:
1911 1753 958 919 550 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2994 1056
Size: 1938 5128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2625 2935 3169 3425 3539 5726 1239
Size: 1221 165 310 234 256 114 2187 2579
Fragments arranged by size:
2579 2187 1221 310 256 234 165 114
Bpu10I CC'TnA_GC
Cuts at: 2257 2385 2412 2460 2257
Size: 128 27 48 6863
Fragments arranged by size:
6863 128 48 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2597 2846 3836 4118 4192 5191 6056 6354 2597
Size: 249 990 282 74 999 865 298 3309
Fragments arranged by size:
3309 999 990 865 298 282 249 74
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 3779 5729 882
Size: 617 1116 1164 1950 2219
Fragments arranged by size:
2219 1950 1164 1116 617
BsaAI yAC'GTr
Cuts at: 493 1204 2239 4363 4857 493
Size: 711 1035 2124 494 2702
Fragments arranged by size:
2702 2124 1035 711 494
BsaBI GATnn'nnATC
Cuts at: 7060 7060
Size: 7066
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 3062 3368 4873
Size: 53 83 186 936 581 948 306 1505
Cuts at: 4873 5255 5890 275
Size: 382 635 1451
Fragments arranged by size:
1505 1451 948 936 635 581 382 306
186 83 53
BsaWI w'CCGG_w
Cuts at: 5499 5897 6171 6318 6729 5499
Size: 398 274 147 411 5836
Fragments arranged by size:
5836 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3155 3185 3215 3245 3455
Size: 30 165 30 1752 30 30 30 210
Cuts at: 3455 3485 1178
Size: 30 4759
Fragments arranged by size:
4759 1752 210 165 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2846 3169 3172 4229 1186
Size: 1660 323 3 1057 4023
Fragments arranged by size:
4023 1660 1057 323 3
BseYI C'CCAG_C
Cuts at: 2084 2700 3051 3071 6269 6765 2084
Size: 616 351 20 3198 496 2385
Fragments arranged by size:
3198 2385 616 496 351 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3767 1163
Size: 573 2031 4462
Fragments arranged by size:
4462 2031 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3307 4516 5277 6305 1540
Size: 275 186 1084 222 1209 761 1028 2301
Fragments arranged by size:
2301 1209 1084 1028 761 275 222 186
BsiHKAI G_wGCw'C
Cuts at: 2233 5127 5212 6283 2233
Size: 2894 85 1071 3016
Fragments arranged by size:
3016 2894 1071 85
BsmI GAATG_Cn'
Cuts at: 4301 4577 4670 4301
Size: 276 93 6697
Fragments arranged by size:
6697 276 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 4210 2105
Size: 2105 4961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2440 2505 2928 2966 3046 3091
Size: 399 443 207 65 423 38 80 45
Cuts at: 3091 3316 3495 3569 3613 4033 4385 5127 5212
Size: 225 179 74 44 420 352 742 85
Cuts at: 5212 6283 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 443 423 420 399 352
225 207 179 85 80 74 65 45
44 38
BspEI T'CCGG_A
Cuts at: 6729 6729
Size: 7066
BspHI T'CATG_A
Cuts at: 3132 4955 6685 3132
Size: 1823 1730 3513
Fragments arranged by size:
3513 1823 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2554 3578 3603 4421 844
Size: 1710 1024 25 818 3489
Fragments arranged by size:
3489 1710 1024 818 25
BsrBI CCG'CTC
Cuts at: 1394 1823 3171 3177 4953 1394
Size: 429 1348 6 1776 3507
Fragments arranged by size:
3507 1776 1348 429 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 2218 3889 5561 5735 65
Size: 1183 970 1671 1672 174 1396
Fragments arranged by size:
1672 1671 1396 1183 970 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 3080 5710 5897 1700
Size: 110 376 894 2630 187 2869
Fragments arranged by size:
2869 2630 894 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7066
BssSI C'ACGA_G
Cuts at: 2123 3972 4094 5126 6138 2123
Size: 1849 122 1032 1012 3051
Fragments arranged by size:
3051 1849 1032 1012 122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3553 3595 794
Size: 2759 42 4265
Fragments arranged by size:
4265 2759 42
BstBI TT'CG_AA
Cuts at: 2007 5870 2007
Size: 3863 3203
BstEII G'GTnAC_C
Cuts at: 1184 3815 1184
Size: 2631 4435
BstXI CCAn_nnnn'nTGG
Cuts at: 2522 2660 2687 3958 2522
Size: 138 27 1271 5630
Fragments arranged by size:
5630 1271 138 27
BstYI r'GATC_y
Cuts at: 1972 3736 3988 4396 4763 5148 5165 6617 6703
Size: 1764 252 408 367 385 17 1452 86
Cuts at: 6703 6715 7061 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1764 1452 408 385 367 346 252
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3291 3291
Size: 7066
BtgI C'CryG_G
Cuts at: 513 1065 2022 2508 3951 513
Size: 552 957 486 1443 3628
Fragments arranged by size:
3628 1443 957 552 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6327
Fragments arranged by size:
6327 683 56
BtsI GCAGTG_nn'
Cuts at: 3532 4582 5376 5396 3532
Size: 1050 794 20 5202
Fragments arranged by size:
5202 1050 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2555 2590 529
Size: 35 1991 35 5005
Fragments arranged by size:
5005 1991 35 35
DraI TTT'AAA
Cuts at: 4370 4370
Size: 7066
DraIII CAC_nnn'GTG
Cuts at: 3268 3268
Size: 7066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6073 809
Size: 507 4757 1802
Fragments arranged by size:
4757 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2160 2223
Size: 54 1425 50 275 18 198 132 63
Cuts at: 2223 4513 5402 7031 8
Size: 2290 889 1629 43
Fragments arranged by size:
2290 1629 1425 889 275 198 132 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4513 1537
Size: 275 2701 4090
Fragments arranged by size:
4090 2701 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 3347 3819 4190 4354 4996 1353
Size: 1801 193 472 371 164 642 3423
Fragments arranged by size:
3423 1801 642 472 371 193 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4187 5653 6027 6173 387
Size: 1064 138 2598 1466 374 146 1280
Fragments arranged by size:
2598 1466 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 2625 2935 3169 3425 3452 3539
Size: 393 828 165 310 234 256 27 87
Cuts at: 3539 4001 5129 5726 6513 6781 1239
Size: 462 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 828 787 597 462 393 310
268 256 234 165 87 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3009 3184 3009
Size: 175 6891
EcoO109I rG'GnC_Cy
Cuts at: 2155 2501 2736 3189 3312 3415 4030 2155
Size: 346 235 453 123 103 615 5191
Fragments arranged by size:
5191 615 453 346 235 123 103
EcoRI G'AATT_C
Cuts at: 4375 4753 4375
Size: 378 6688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 7066
FspI TGC'GCA
Cuts at: 5572 5572
Size: 7066
HaeII r_GCGC'y
Cuts at: 1678 2809 3371 3404 6213 1678
Size: 1131 562 33 2809 2531
Fragments arranged by size:
2809 2531 1131 562 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2593 2625 3131
Size: 32 135 32 779 32 210 32 506
Cuts at: 3131 3155 3163 3187 3209 3241 3346 3378 5707
Size: 24 8 24 22 32 105 32 2329
Cuts at: 5707 5739 5781 5813 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2329 779 506 210 135 105 42
32 32 32 32 32 32 32 32
24 24 22 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4410 4656 669
Size: 988 281 2472 246 3079
Fragments arranged by size:
3079 2472 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7066
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 7066
KasI G'GCGC_C
Cuts at: 3367 3367
Size: 7066
KpnI G_GTAC'C
Cuts at: 2293 4391 2293
Size: 2098 4968
MfeI C'AATT_G
Cuts at: 4665 4665
Size: 7066
MluI A'CGCG_T
Cuts at: 3699 6942 3699
Size: 3243 3823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 2808 3258 6180 6364 1305
Size: 1386 117 450 2922 184 2007
Fragments arranged by size:
2922 2007 1386 450 184 117
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2225 10
Size: 54 1425 343 330 63 4851
Fragments arranged by size:
4851 1425 343 330 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5024 5383 5542 518
Size: 648 3858 359 159 2042
Fragments arranged by size:
3858 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 2572 2733 4482 5159 6307 6552 1606
Size: 786 180 161 1749 677 1148 245 2120
Fragments arranged by size:
2120 1749 1148 786 677 245 180 161
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 7066
NarI GG'CG_CC
Cuts at: 3368 3368
Size: 7066
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5557
Fragments arranged by size:
5557 957 552
NdeI CA'TA_TG
Cuts at: 387 3318 387
Size: 2931 4135
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 7066
NheI G'CTAG_C
Cuts at: 1052 2990 1052
Size: 1938 5128
NotI GC'GGCC_GC
Cuts at: 4513 4513
Size: 7066
NspI r_CATG'y
Cuts at: 1132 1229 2368 4084 4424 5969 1132
Size: 97 1139 1716 340 1545 2229
Fragments arranged by size:
2229 1716 1545 1139 340 97
PasI CC'CwG_GG
Cuts at: 1348 2597 4033 1348
Size: 1249 1436 4381
Fragments arranged by size:
4381 1436 1249
PciI A'CATG_T
Cuts at: 5965 5965
Size: 7066
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2913 4009 1108
Size: 1805 1096 4165
Fragments arranged by size:
4165 1805 1096
PmeI GTTT'AAAC
Cuts at: 4370 4370
Size: 7066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5135 5167 6648 6680 5135
Size: 32 1481 32 5521
Fragments arranged by size:
5521 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2736 3189 3415 2736
Size: 453 226 6387
Fragments arranged by size:
6387 453 226
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7066
PsiI TTA'TAA
Cuts at: 4636 4636
Size: 7066
PspOMI G'GGCC_C
Cuts at: 2501 3312 4029 2501
Size: 811 717 5538
Fragments arranged by size:
5538 811 717
PspXI vC'TCGA_Gb
Cuts at: 3488 3488
Size: 7066
PstI C_TGCA'G
Cuts at: 830 2877 2889 3507 3558 4418 830
Size: 2047 12 618 51 860 3478
Fragments arranged by size:
3478 2047 860 618 51 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7066
PvuII CAG'CTG
Cuts at: 2392 2572 2392
Size: 180 6886
SalI G'TCGA_C
Cuts at: 1936 4408 1936
Size: 2472 4594
SapI GCTCTTCn'nnn_
Cuts at: 3154 4190 3154
Size: 1036 6030
SbfI CC_TGCA'GG
Cuts at: 4418 4418
Size: 7066
ScaI AGT'ACT
Cuts at: 2279 5314 7026 2279
Size: 3035 1712 2319
Fragments arranged by size:
3035 2319 1712
SfcI C'TryA_G
Cuts at: 826 1045 2317 2873 2885 3503 3554 4414 5549
Size: 219 1272 556 12 618 51 860 1135
Cuts at: 5549 6230 6421 826
Size: 681 191 1471
Fragments arranged by size:
1471 1272 1135 860 681 618 556 219
191 51 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2153 2153
Size: 7066
SfoI GGC'GCC
Cuts at: 3369 3369
Size: 7066
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7066
SmaI CCC'GGG
Cuts at: 1872 2747 2945 3067 3092 4393 1872
Size: 875 198 122 25 1301 4545
Fragments arranged by size:
4545 1301 875 198 122 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2612 2861 3488 3851 4133 4207
Size: 197 926 669 249 627 363 282 74
Cuts at: 4207 5170 6071 6333 820
Size: 963 901 262 1553
Fragments arranged by size:
1553 963 926 901 669 627 363 282
262 249 197 74
SnaBI TAC'GTA
Cuts at: 493 4363 4857 493
Size: 3870 494 2702
Fragments arranged by size:
3870 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7066
SphI G_CATG'C
Cuts at: 1132 4424 1132
Size: 3292 3774
SspI AAT'ATT
Cuts at: 5 52 4990 5
Size: 47 4938 2081
Fragments arranged by size:
4938 2081 47
StuI AGG'CCT
Cuts at: 1517 2519 1517
Size: 1002 6064
StyI C'CwwG_G
Cuts at: 513 1065 2022 3804 4008 4310 4505 513
Size: 552 957 1782 204 302 195 3074
Fragments arranged by size:
3074 1782 957 552 302 204 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 2666 5093 5110 5263 5448 5893
Size: 1255 447 639 2427 17 153 185 445
Cuts at: 5893 325
Size: 1498
Fragments arranged by size:
2427 1498 1255 639 447 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2070 2277 2298
Size: 274 80 33 51 1443 93 207 21
Cuts at: 2298 5312 7024 96
Size: 3014 1712 138
Fragments arranged by size:
3014 1712 1443 274 207 138 93 80
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2720 2752 3215 3247 3455 3487 7026 7058 2720
Size: 32 463 32 208 32 3539 32 2728
Fragments arranged by size:
3539 2728 463 208 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7066
XbaI T'CTAG_A
Cuts at: 4402 4402
Size: 7066
XhoI C'TCGA_G
Cuts at: 1943 3488 1943
Size: 1545 5521
XmaI C'CCGG_G
Cuts at: 1870 2745 2943 3065 3090 4391 1870
Size: 875 198 122 25 1301 4545
Fragments arranged by size:
4545 1301 875 198 122 25
XmnI GAAnn'nnTTC
Cuts at: 5195 5195
Size: 7066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4874 276
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PsrI RsrII SacI SacII SanDI SexAI SrfI
SwaI XcmI