Restriction map for
pFN21ASDA1917
(Circular) (Six-base) MAPSORT of: pFN21ASDA1917.seq Check: 2612 from: 1 to: 6766
pFN21ASDA1917 6766 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3249 3249
Size: 6766
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4576 278
Size: 53 83 186 936 3040 2468
Fragments arranged by size:
3040 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2745 4059 4109 1937
Size: 808 1314 50 4594
Fragments arranged by size:
4594 1314 808 50
Acc65I G'GTAC_C
Cuts at: 4087 4087
Size: 6766
AclI AA'CG_TT
Cuts at: 1563 4893 5266 1563
Size: 3330 373 3063
Fragments arranged by size:
3330 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 2750 2841 2975 3096 3675 4829 6213
Size: 558 560 91 134 121 579 1154 1384
Cuts at: 6213 6481 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1154 579 560 558 268 134
121 91
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6569
AflIII A'CryG_T
Cuts at: 1203 5665 6642 1203
Size: 4462 977 1327
Fragments arranged by size:
4462 1327 977
AgeI A'CCGG_T
Cuts at: 5597 5597
Size: 6766
AhdI GACnn_n'nnGTC
Cuts at: 2074 3277 3499 5495 2074
Size: 1203 222 1996 3345
Fragments arranged by size:
3345 1996 1203 222
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6766
AlwNI CAG_nnn'CTG
Cuts at: 4509 6081 4509
Size: 1572 5194
ApaI G_GGCC'C
Cuts at: 2032 3023 3150 3703 4049 2032
Size: 991 127 553 346 4749
Fragments arranged by size:
4749 991 553 346 127
ApaLI G'TGCA_C
Cuts at: 4823 5979 4823
Size: 1156 5610
ApoI r'AATT_y
Cuts at: 1493 4075 4301 4453 6562 1493
Size: 2582 226 152 2109 1697
Fragments arranged by size:
2582 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5320 160
Size: 872 4288 1606
Fragments arranged by size:
4288 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
AvaI C'yCGr_G
Cuts at: 1870 1943 2406 4091 1870
Size: 73 463 1685 4545
Fragments arranged by size:
4545 1685 463 73
BamHI G'GATC_C
Cuts at: 2557 4096 4463 2557
Size: 1539 367 4860
Fragments arranged by size:
4860 1539 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3944 4087 5542 618
Size: 325 217 627 2157 143 1455 1842
Fragments arranged by size:
2157 1842 1455 627 325 217 143
BanII G_rGCy'C
Cuts at: 1391 2032 2342 3023 3150 3703 4049 4085 1391
Size: 641 310 681 127 553 346 36 4072
Fragments arranged by size:
4072 681 641 553 346 310 127 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2273 2829 928
Size: 971 374 556 4865
Fragments arranged by size:
4865 971 556 374
BbvCI CC'TCA_GC
Cuts at: 2325 2325
Size: 6766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4957 4991 1903
Size: 34 3020 34 3678
Fragments arranged by size:
3678 3020 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4658 5868 4658
Size: 1210 5556
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6766
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3698 3953 5377
Size: 107 122 71 631 605 2026 255 1424
Cuts at: 5377 136
Size: 1525
Fragments arranged by size:
2026 1525 1424 631 605 255 122 107
71
BglII A'GATC_T
Cuts at: 2772 6761 2772
Size: 3989 2777
BlpI GC'TnA_GC
Cuts at: 4183 4183
Size: 6766
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2986 3023 3150 3703 4049 4827 5983
Size: 242 954 37 127 553 346 778 1156
Cuts at: 5983 1790
Size: 2573
Fragments arranged by size:
2573 1156 954 778 553 346 242 127
37
BmgBI CAC'GTC
Cuts at: 1318 3843 1318
Size: 2525 4241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3565 4497 5455
Size: 416 53 83 399 24 2148 932 958
Cuts at: 5455 442
Size: 1753
Fragments arranged by size:
2148 1753 958 932 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3691 3689
Size: 2 6764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2364 2474 3152 3435 3504 3723 3789 4034
Size: 1125 110 678 283 69 219 66 245
Cuts at: 4034 5426 1239
Size: 1392 2579
Fragments arranged by size:
2579 1392 1125 678 283 245 219 110
69 66
Bpu10I CC'TnA_GC
Cuts at: 2325 2325
Size: 6766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4891 5756 6054 4891
Size: 865 298 5603
Fragments arranged by size:
5603 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3403 5429 882
Size: 617 1904 2026 2219
Fragments arranged by size:
2219 2026 1904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3184 4063 4557 493
Size: 711 1980 879 494 2702
Fragments arranged by size:
2702 1980 879 711 494
BsaBI GATnn'nnATC
Cuts at: 6760 6760
Size: 6766
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3515 4573 4955 5590
Size: 53 83 186 936 1982 1058 382 635
Cuts at: 5590 275
Size: 1451
Fragments arranged by size:
1982 1451 1058 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2108 5199 5597 5871 6018 6429 2108
Size: 3091 398 274 147 411 2445
Fragments arranged by size:
3091 2445 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3602 3632 1178
Size: 30 165 30 2199 30 4312
Fragments arranged by size:
4312 2199 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2157 2532 2594 3610 3674 1186
Size: 889 82 375 62 1016 64 4278
Fragments arranged by size:
4278 1016 889 375 82 64 62
BseYI C'CCAG_C
Cuts at: 2677 3163 3684 3763 3984 5969 6465 2677
Size: 486 521 79 221 1985 496 2978
Fragments arranged by size:
2978 1985 521 496 486 221 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2525 2946 1163
Size: 573 789 421 4983
Fragments arranged by size:
4983 789 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4216 4977 6005 1540
Size: 275 186 2215 761 1028 2301
Fragments arranged by size:
2301 2215 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3926 4827 4912 5983 3926
Size: 901 85 1071 4709
Fragments arranged by size:
4709 1071 901 85
BsmI GAATG_Cn'
Cuts at: 3216 3373 3985 4277 4370 3216
Size: 157 612 292 93 5612
Fragments arranged by size:
5612 612 292 157 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2640 3622 4019 2640
Size: 982 397 5387
Fragments arranged by size:
5387 982 397
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2342 2986 3023 3150 3703 3926
Size: 399 242 310 644 37 127 553 223
Cuts at: 3926 4049 4085 4827 4912 5983 1391
Size: 123 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 553 399 310 242
223 127 123 85 37 36
BspEI T'CCGG_A
Cuts at: 2108 6429 2108
Size: 4321 2445
BspHI T'CATG_A
Cuts at: 4655 6385 4655
Size: 1730 5036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3249 4121 844
Size: 2405 872 3489
Fragments arranged by size:
3489 2405 872
BsrBI CCG'CTC
Cuts at: 1394 1823 4653 1394
Size: 429 2830 3507
Fragments arranged by size:
3507 2830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2662 5261 5435 65
Size: 1183 1414 2599 174 1396
Fragments arranged by size:
2599 1414 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 3990 5410 5597 1700
Size: 110 230 1950 1420 187 2869
Fragments arranged by size:
2869 1950 1420 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6766
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6766
BssSI C'ACGA_G
Cuts at: 4826 5838 4826
Size: 1012 5754
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6766
BstBI TT'CG_AA
Cuts at: 2007 5570 2007
Size: 3563 3203
BstEII G'GTnAC_C
Cuts at: 1184 2174 1184
Size: 990 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 4012 4012
Size: 6766
BstYI r'GATC_y
Cuts at: 1972 2557 2772 2994 4096 4463 4848 4865 6317
Size: 585 215 222 1102 367 385 17 1452
Cuts at: 6317 6403 6415 6761 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1102 585 385 367 346 222
215 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2436 2553 2631 3585 513
Size: 552 957 414 117 78 954 3694
Fragments arranged by size:
3694 957 954 552 414 117 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3625 3874 487
Size: 683 56 2399 249 3379
Fragments arranged by size:
3379 2399 683 249 56
BtsI GCAGTG_nn'
Cuts at: 2984 3235 4282 5076 5096 2984
Size: 251 1047 794 20 4654
Fragments arranged by size:
4654 1047 794 251 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6731
DraI TTT'AAA
Cuts at: 4070 4070
Size: 6766
DraIII CAC_nnn'GTG
Cuts at: 2554 2554
Size: 6766
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3520 3968 5773 809
Size: 507 2204 448 1805 1802
Fragments arranged by size:
2204 1805 1802 507 448
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3196 3235
Size: 54 1425 50 275 18 829 537 39
Cuts at: 3235 4213 5102 6731 8
Size: 978 889 1629 43
Fragments arranged by size:
1629 1425 978 889 829 537 275 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 4213 1537
Size: 275 2401 4090
Fragments arranged by size:
4090 2401 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2343 2436 2571 3072 3132 3349 3354
Size: 977 13 93 135 501 60 217 5
Cuts at: 3354 3651 3682 3801 4696 1353
Size: 297 31 119 895 3423
Fragments arranged by size:
3423 977 895 501 297 217 135 119
93 60 31 13 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5353 5727 5873 387
Size: 1064 138 3764 374 146 1280
Fragments arranged by size:
3764 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2364 2474 2750 2841 2975 3096
Size: 393 558 174 110 276 91 134 121
Cuts at: 3096 3152 3435 3504 3675 3723 3789 4034 4829
Size: 56 283 69 171 48 66 245 795
Cuts at: 4829 5426 6213 6481 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 558 393 283 276
268 245 174 171 134 121 110 91
69 66 56 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2765
Size: 6766
EcoO109I rG'GnC_Cy
Cuts at: 2028 2029 3020 3136 3147 3548 3699 3700 4032
Size: 1 991 116 11 401 151 1 332
Cuts at: 4032 4045 4046 2028
Size: 13 1 4748
Fragments arranged by size:
4748 991 401 332 151 116 13 11
1 1 1
EcoRI G'AATT_C
Cuts at: 4075 4453 4075
Size: 378 6388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2600 2600
Size: 6766
FspI TGC'GCA
Cuts at: 3847 5272 3847
Size: 1425 5341
FspAI rTGC'GCAy
Cuts at: 3847 3847
Size: 6766
HaeII r_GCGC'y
Cuts at: 1678 3991 3997 5913 1678
Size: 2313 6 1916 2531
Fragments arranged by size:
2531 2313 1916 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 3071 3103 3250
Size: 32 135 32 488 32 979 32 147
Cuts at: 3250 3263 3282 3291 3295 3323 3485 3517 3602
Size: 13 19 9 4 28 162 32 85
Cuts at: 3602 3634 3800 3832 5407 5439 5481 5513 1373
Size: 32 166 32 1575 32 42 32 2626
Fragments arranged by size:
2626 1575 979 488 166 162 147 135
85 42 32 32 32 32 32 32
32 32 32 28 19 13 9 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4110 4356 669
Size: 988 281 2172 246 3079
Fragments arranged by size:
3079 2172 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6766
HpaI GTT'AAC
Cuts at: 4356 4356
Size: 6766
KpnI G_GTAC'C
Cuts at: 4091 4091
Size: 6766
MfeI C'AATT_G
Cuts at: 4365 4365
Size: 6766
MluI A'CGCG_T
Cuts at: 6642 6642
Size: 6766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 3369 3956 5880 6064 1305
Size: 1170 894 587 1924 184 2007
Fragments arranged by size:
2007 1924 1170 894 587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2661 3198 3237 10
Size: 54 1425 343 829 537 39 3539
Fragments arranged by size:
3539 1425 829 537 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2376 2667 2700 3584 4724 5083 5242
Size: 648 1210 291 33 884 1140 359 159
Cuts at: 5242 518
Size: 2042
Fragments arranged by size:
2042 1210 1140 884 648 359 291 159
33
MspA1I CmG'CkG
Cuts at: 1606 3731 3767 4182 4859 6007 6252 1606
Size: 2125 36 415 677 1148 245 2120
Fragments arranged by size:
2125 2120 1148 677 415 245 36
NaeI GCC'GGC
Cuts at: 2042 3992 2042
Size: 1950 4816
NcoI C'CATG_G
Cuts at: 513 1065 2022 3585 513
Size: 552 957 1563 3694
Fragments arranged by size:
3694 1563 957 552
NdeI CA'TA_TG
Cuts at: 387 3223 387
Size: 2836 3930
NgoMIV G'CCGG_C
Cuts at: 2040 3990 2040
Size: 1950 4816
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6766
NotI GC'GGCC_GC
Cuts at: 4213 4213
Size: 6766
NspI r_CATG'y
Cuts at: 1132 1229 2630 4124 5669 1132
Size: 97 1401 1494 1545 2229
Fragments arranged by size:
2229 1545 1494 1401 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6766
PciI A'CATG_T
Cuts at: 5665 5665
Size: 6766
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2554 1108
Size: 1446 5320
PfoI T'CCnGG_A
Cuts at: 2874 2874
Size: 6766
PmeI GTTT'AAAC
Cuts at: 4070 4070
Size: 6766
PmlI CAC'GTG
Cuts at: 3184 3184
Size: 6766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4835 4867 6348 6380 4835
Size: 32 1481 32 5221
Fragments arranged by size:
5221 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6766
PsiI TTA'TAA
Cuts at: 4336 4336
Size: 6766
PspOMI G'GGCC_C
Cuts at: 2028 3019 3146 3699 4045 2028
Size: 991 127 553 346 4749
Fragments arranged by size:
4749 991 553 346 127
PstI C_TGCA'G
Cuts at: 830 3383 4118 830
Size: 2553 735 3478
Fragments arranged by size:
3478 2553 735
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6766
PvuII CAG'CTG
Cuts at: 3767 3767
Size: 6766
SalI G'TCGA_C
Cuts at: 1936 4108 1936
Size: 2172 4594
SapI GCTCTTCn'nnn_
Cuts at: 2330 3072 2330
Size: 742 6024
SbfI CC_TGCA'GG
Cuts at: 4118 4118
Size: 6766
ScaI AGT'ACT
Cuts at: 5014 6726 5014
Size: 1712 5054
SexAI A'CCwGG_T
Cuts at: 3940 3940
Size: 6766
SfcI C'TryA_G
Cuts at: 826 1045 3379 4114 5249 5930 6121 826
Size: 219 2334 735 1135 681 191 1471
Fragments arranged by size:
2334 1471 1135 735 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
SgrAI Cr'CCGG_yG
Cuts at: 1700 3990 1700
Size: 2290 4476
SmaI CCC'GGG
Cuts at: 1872 4093 1872
Size: 2221 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4870 5771 6033 820
Size: 197 926 2927 901 262 1553
Fragments arranged by size:
2927 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4063 4557 493
Size: 3570 494 2702
Fragments arranged by size:
3570 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6766
SphI G_CATG'C
Cuts at: 1132 4124 1132
Size: 2992 3774
SspI AAT'ATT
Cuts at: 5 52 3100 4690 5
Size: 47 3048 1590 2081
Fragments arranged by size:
3048 2081 1590 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6766
StyI C'CwwG_G
Cuts at: 513 1065 2022 3585 4205 513
Size: 552 957 1563 620 3074
Fragments arranged by size:
3074 1563 957 620 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2616 3835 4793 4810 4963 5148 5593
Size: 1255 1036 1219 958 17 153 185 445
Cuts at: 5593 325
Size: 1498
Fragments arranged by size:
1498 1255 1219 1036 958 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 2671 2793
Size: 274 80 33 51 1443 531 163 122
Cuts at: 2793 5012 6724 96
Size: 2219 1712 138
Fragments arranged by size:
2219 1712 1443 531 274 163 138 122
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2542 2574 6726 6758 2542
Size: 32 4152 32 2550
Fragments arranged by size:
4152 2550 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2750 3614 1316
Size: 1434 864 4468
Fragments arranged by size:
4468 1434 864
XbaI T'CTAG_A
Cuts at: 4102 4102
Size: 6766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3562 3562
Size: 6766
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6766
XmaI C'CCGG_G
Cuts at: 1870 4091 1870
Size: 2221 4545
XmnI GAAnn'nnTTC
Cuts at: 4895 4895
Size: 6766
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4574 276
Size: 53 83 186 936 3040 2468
Fragments arranged by size:
3040 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FseI KasI NarI NruI
NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI