Restriction map for
pFN21ASDA1911
(Circular) (Six-base) MAPSORT of: pFN21ASDA1911.seq Check: 596 from: 1 to: 7249
pFN21ASDA1911 7249 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5059 278
Size: 53 83 186 936 3523 2468
Fragments arranged by size:
3523 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3151 3899 4592 1937
Size: 1214 748 693 4594
Fragments arranged by size:
4594 1214 748 693
Acc65I G'GTAC_C
Cuts at: 4570 4570
Size: 7249
AclI AA'CG_TT
Cuts at: 1563 5376 5749 1563
Size: 3813 373 3063
Fragments arranged by size:
3813 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 3423 4059 5312 6696 6964 1632
Size: 1134 657 636 1253 1384 268 1917
Fragments arranged by size:
1917 1384 1253 1134 657 636 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7052
AflIII A'CryG_T
Cuts at: 1203 2542 6148 7125 1203
Size: 1339 3606 977 1327
Fragments arranged by size:
3606 1339 1327 977
AgeI A'CCGG_T
Cuts at: 6080 6080
Size: 7249
AhdI GACnn_n'nnGTC
Cuts at: 5978 5978
Size: 7249
AleI CACnn'nnGTG
Cuts at: 1166 4123 1166
Size: 2957 4292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7249
AlwNI CAG_nnn'CTG
Cuts at: 2876 3934 4313 4992 6564 2876
Size: 1058 379 679 1572 3561
Fragments arranged by size:
3561 1572 1058 679 379
ApaLI G'TGCA_C
Cuts at: 5306 6462 5306
Size: 1156 6093
ApoI r'AATT_y
Cuts at: 1493 2053 2089 2103 2442 2617 3215 3553 3659
Size: 560 36 14 339 175 598 338 106
Cuts at: 3659 3993 4558 4784 4936 7045 1493
Size: 334 565 226 152 2109 1697
Fragments arranged by size:
2109 1697 598 565 560 339 338 334
226 175 152 106 36 14
AseI AT'TA_AT
Cuts at: 160 1032 2271 2604 3396 3417 3438 4524 5803
Size: 872 1239 333 792 21 21 1086 1279
Cuts at: 5803 160
Size: 1606
Fragments arranged by size:
1606 1279 1239 1086 872 792 333 21
21
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
AvaI C'yCGr_G
Cuts at: 1870 1943 4574 1870
Size: 73 2631 4545
Fragments arranged by size:
4545 2631 73
BamHI G'GATC_C
Cuts at: 4579 4946 4579
Size: 367 6882
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4570 6025 618
Size: 325 217 627 2783 1455 1842
Fragments arranged by size:
2783 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3460 4568 1391
Size: 2069 1108 4072
Fragments arranged by size:
4072 2069 1108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2717 4047 928
Size: 971 818 1330 4130
Fragments arranged by size:
4130 1330 971 818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2722 2756 3092 3126 5440 5474 1903
Size: 34 785 34 336 34 2314 34 3678
Fragments arranged by size:
3678 2314 785 336 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5141 6351 5141
Size: 1210 6039
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7249
BfrBI ATG'CAT
Cuts at: 3310 3310
Size: 7249
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5860 136
Size: 107 122 71 631 605 4188 1525
Fragments arranged by size:
4188 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7244 7244
Size: 7249
BlpI GC'TnA_GC
Cuts at: 3172 4666 3172
Size: 1494 5755
Bme1580I G_kGCm'C
Cuts at: 1790 5310 6466 1790
Size: 3520 1156 2573
Fragments arranged by size:
3520 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4980 5938 442
Size: 416 53 83 399 24 3563 958 1753
Fragments arranged by size:
3563 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 7249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2918 5909 1239
Size: 1679 2991 2579
Fragments arranged by size:
2991 2579 1679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3234 5374 6239 6537 3234
Size: 2140 865 298 3946
Fragments arranged by size:
3946 2140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3044 5912 882
Size: 617 1545 2868 2219
Fragments arranged by size:
2868 2219 1545 617
BsaAI yAC'GTr
Cuts at: 493 1204 4542 4546 5040 493
Size: 711 3338 4 494 2702
Fragments arranged by size:
3338 2702 711 494 4
BsaBI GATnn'nnATC
Cuts at: 2592 7243 2592
Size: 4651 2598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5056 5438 6073 275
Size: 53 83 186 936 3523 382 635 1451
Fragments arranged by size:
3523 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2379 5682 6080 6354 6501 6912 2379
Size: 3303 398 274 147 411 2716
Fragments arranged by size:
3303 2716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7024
Fragments arranged by size:
7024 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7249
BseYI C'CCAG_C
Cuts at: 6452 6948 6452
Size: 496 6753
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3248 1163
Size: 573 1512 5164
Fragments arranged by size:
5164 1512 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4699 5460 6488 1540
Size: 275 186 2698 761 1028 2301
Fragments arranged by size:
2698 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5310 5395 6466 5310
Size: 85 1071 6093
Fragments arranged by size:
6093 1071 85
BsiWI C'GTAC_G
Cuts at: 4542 4542
Size: 7249
BsmI GAATG_Cn'
Cuts at: 3897 4394 4760 4853 3897
Size: 497 366 93 6293
Fragments arranged by size:
6293 497 366 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2718 2718
Size: 7249
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3460 4568 5310 5395 6466 1391
Size: 399 1670 1108 742 85 1071 2174
Fragments arranged by size:
2174 1670 1108 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6912 6912
Size: 7249
BspHI T'CATG_A
Cuts at: 5138 6868 5138
Size: 1730 5519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4604 844
Size: 3760 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5136 1394
Size: 429 3313 3507
Fragments arranged by size:
3507 3313 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3110 5744 5918 65
Size: 1183 1862 2634 174 1396
Fragments arranged by size:
2634 1862 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5893 6080 1700
Size: 110 4083 187 2869
Fragments arranged by size:
4083 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7249
BssSI C'ACGA_G
Cuts at: 5309 6321 5309
Size: 1012 6237
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7249
BstBI TT'CG_AA
Cuts at: 2007 6053 2007
Size: 4046 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7249
BstYI r'GATC_y
Cuts at: 1972 2690 4450 4579 4946 5331 5348 6800 6886
Size: 718 1760 129 367 385 17 1452 86
Cuts at: 6886 6898 7244 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1760 1452 718 385 367 346 129
86 17 12
BstZ17I GTA'TAC
Cuts at: 3152 3900 3152
Size: 748 6501
Bsu36I CC'TnA_GG
Cuts at: 4394 4394
Size: 7249
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6510
Fragments arranged by size:
6510 683 56
BtsI GCAGTG_nn'
Cuts at: 2504 3724 4765 5559 5579 2504
Size: 1220 1041 794 20 4174
Fragments arranged by size:
4174 1220 1041 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7214
DraI TTT'AAA
Cuts at: 4553 4553
Size: 7249
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6256 809
Size: 507 4940 1802
Fragments arranged by size:
4940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3477 4696 5585
Size: 54 1425 50 275 18 1647 1219 889
Cuts at: 5585 7214 8
Size: 1629 43
Fragments arranged by size:
1647 1629 1425 1219 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4696 1537
Size: 275 2884 4090
Fragments arranged by size:
4090 2884 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 2716 3042 4157 4221 5179 1353
Size: 1190 173 326 1115 64 958 3423
Fragments arranged by size:
3423 1190 1115 958 326 173 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5836 6210 6356 387
Size: 1064 138 4247 374 146 1280
Fragments arranged by size:
4247 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2766 2918 3423 4059 5312 5909 6696
Size: 393 1134 152 505 636 1253 597 787
Cuts at: 6696 6964 1239
Size: 268 1524
Fragments arranged by size:
1524 1253 1134 787 636 597 505 393
268 152
EcoNI CCTnn'n_nnAGG
Cuts at: 3264 4061 3264
Size: 797 6452
EcoRI G'AATT_C
Cuts at: 2053 2089 3993 4558 4936 2053
Size: 36 1904 565 378 4366
Fragments arranged by size:
4366 1904 565 378 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4250 4250
Size: 7249
FspI TGC'GCA
Cuts at: 5755 5755
Size: 7249
HaeII r_GCGC'y
Cuts at: 1678 6396 1678
Size: 4718 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3527 3559 5890 5922 5964
Size: 32 135 32 1955 32 2331 32 42
Cuts at: 5964 5996 1373
Size: 32 2626
Fragments arranged by size:
2626 2331 1955 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4069 4090 4593 4839 669
Size: 988 281 2131 21 503 246 3079
Fragments arranged by size:
3079 2131 988 503 281 246 21
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7249
HpaI GTT'AAC
Cuts at: 4839 4839
Size: 7249
KpnI G_GTAC'C
Cuts at: 4574 4574
Size: 7249
MfeI C'AATT_G
Cuts at: 4848 4848
Size: 7249
MluI A'CGCG_T
Cuts at: 7125 7125
Size: 7249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3213 3222 4340 6363 6547 1305
Size: 1908 9 1118 2023 184 2007
Fragments arranged by size:
2023 2007 1908 1118 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3479 10
Size: 54 1425 343 1647 3780
Fragments arranged by size:
3780 1647 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4123 5207 5566 5725 518
Size: 648 2957 1084 359 159 2042
Fragments arranged by size:
2957 2042 1084 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4665 5342 6490 6735 1606
Size: 3059 677 1148 245 2120
Fragments arranged by size:
3059 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6697
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7249
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7249
NotI GC'GGCC_GC
Cuts at: 4696 4696
Size: 7249
NsiI A_TGCA'T
Cuts at: 3312 3312
Size: 7249
NspI r_CATG'y
Cuts at: 1132 1229 2546 4607 6152 1132
Size: 97 1317 2061 1545 2229
Fragments arranged by size:
2229 2061 1545 1317 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7249
PciI A'CATG_T
Cuts at: 2542 6148 2542
Size: 3606 3643
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3568 1108
Size: 2460 4789
PmeI GTTT'AAAC
Cuts at: 4553 4553
Size: 7249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5318 5350 6831 6863 5318
Size: 32 1481 32 5704
Fragments arranged by size:
5704 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7249
PsiI TTA'TAA
Cuts at: 3956 4819 3956
Size: 863 6386
PstI C_TGCA'G
Cuts at: 830 4601 830
Size: 3771 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7249
SalI G'TCGA_C
Cuts at: 1936 4591 1936
Size: 2655 4594
SbfI CC_TGCA'GG
Cuts at: 4601 4601
Size: 7249
ScaI AGT'ACT
Cuts at: 2509 2559 4487 5497 7209 2509
Size: 50 1928 1010 1712 2549
Fragments arranged by size:
2549 1928 1712 1010 50
SfcI C'TryA_G
Cuts at: 826 1045 4213 4597 5732 6413 6604 826
Size: 219 3168 384 1135 681 191 1471
Fragments arranged by size:
3168 1471 1135 681 384 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7249
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7249
SmaI CCC'GGG
Cuts at: 1872 4576 1872
Size: 2704 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3249 5353 6254 6516 820
Size: 197 926 1306 2104 901 262 1553
Fragments arranged by size:
2104 1553 1306 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4546 5040 493
Size: 4053 494 2702
Fragments arranged by size:
4053 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7249
SphI G_CATG'C
Cuts at: 1132 4607 1132
Size: 3475 3774
SspI AAT'ATT
Cuts at: 5 52 5173 5
Size: 47 5121 2081
Fragments arranged by size:
5121 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7249
StyI C'CwwG_G
Cuts at: 513 1065 4688 513
Size: 552 3623 3074
Fragments arranged by size:
3623 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5276 5293 5446 5631 6076 325
Size: 1255 3696 17 153 185 445 1498
Fragments arranged by size:
3696 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2222 2344 2507
Size: 274 80 33 51 1443 245 122 163
Cuts at: 2507 2557 3329 4485 5495 7207 96
Size: 50 772 1156 1010 1712 138
Fragments arranged by size:
1712 1443 1156 1010 772 274 245 163
138 122 80 51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7209 7241 7209
Size: 32 7217
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7249
XbaI T'CTAG_A
Cuts at: 4585 4585
Size: 7249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7249
XmaI C'CCGG_G
Cuts at: 1870 4574 1870
Size: 2704 4545
XmnI GAAnn'nnTTC
Cuts at: 3745 3993 5378 3745
Size: 248 1385 5616
Fragments arranged by size:
5616 1385 248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5057 276
Size: 53 83 186 936 3523 2468
Fragments arranged by size:
3523 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BstXI DraIII EcoICRI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI