Restriction map for
pFN21ASDA1899
(Circular) (Six-base) MAPSORT of: pFN21ASDA1899.seq Check: 7131 from: 1 to: 7801
pFN21ASDA1899 7801 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3336 3767 3336
Size: 431 7370
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5611 278
Size: 53 83 186 936 4075 2468
Fragments arranged by size:
4075 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2580 5144 1937
Size: 643 2564 4594
Fragments arranged by size:
4594 2564 643
Acc65I G'GTAC_C
Cuts at: 5122 5122
Size: 7801
AclI AA'CG_TT
Cuts at: 1563 5928 6301 1563
Size: 4365 373 3063
Fragments arranged by size:
4365 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 2538 3105 3710 3749 4356 5864 7248
Size: 517 389 567 605 39 607 1508 1384
Cuts at: 7248 7516 1632
Size: 268 1917
Fragments arranged by size:
1917 1508 1384 607 605 567 517 389
268 39
AflII C'TTAA_G
Cuts at: 820 1017 2551 4678 820
Size: 197 1534 2127 3943
Fragments arranged by size:
3943 2127 1534 197
AflIII A'CryG_T
Cuts at: 1203 3558 4239 6700 7677 1203
Size: 2355 681 2461 977 1327
Fragments arranged by size:
2461 2355 1327 977 681
AgeI A'CCGG_T
Cuts at: 4839 6632 4839
Size: 1793 6008
AhdI GACnn_n'nnGTC
Cuts at: 6530 6530
Size: 7801
AleI CACnn'nnGTG
Cuts at: 1166 3665 1166
Size: 2499 5302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3734 3930 1184
Size: 2550 196 5055
Fragments arranged by size:
5055 2550 196
AlwNI CAG_nnn'CTG
Cuts at: 3727 5544 7116 3727
Size: 1817 1572 4412
Fragments arranged by size:
4412 1817 1572
ApaI G_GGCC'C
Cuts at: 3124 3645 3124
Size: 521 7280
ApaLI G'TGCA_C
Cuts at: 3464 5858 7014 3464
Size: 2394 1156 4251
Fragments arranged by size:
4251 2394 1156
ApoI r'AATT_y
Cuts at: 1493 2223 2234 2291 2571 4195 4479 4806 5110
Size: 730 11 57 280 1624 284 327 304
Cuts at: 5110 5336 5488 7597 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1624 730 327 304 284 280
226 152 57 11
AseI AT'TA_AT
Cuts at: 160 1032 6355 160
Size: 872 5323 1606
Fragments arranged by size:
5323 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
AvaI C'yCGr_G
Cuts at: 1870 1943 5126 1870
Size: 73 3183 4545
Fragments arranged by size:
4545 3183 73
BamHI G'GATC_C
Cuts at: 2623 5036 5131 5498 2623
Size: 2413 95 367 4926
Fragments arranged by size:
4926 2413 367 95
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3229 3471 3485 5122 6577
Size: 325 217 627 1442 242 14 1637 1455
Cuts at: 6577 618
Size: 1842
Fragments arranged by size:
1842 1637 1455 1442 627 325 242 217
14
BanII G_rGCy'C
Cuts at: 1391 2088 3124 3645 5120 1391
Size: 697 1036 521 1475 4072
Fragments arranged by size:
4072 1475 1036 697 521
BbeI G_GCGC'C
Cuts at: 3489 3489
Size: 7801
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3824 928
Size: 971 1925 4905
Fragments arranged by size:
4905 1925 971
BbvCI CC'TCA_GC
Cuts at: 4424 4424
Size: 7801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3706 3740 3995 4029 4646 4680 5992
Size: 34 1769 34 255 34 617 34 1312
Cuts at: 5992 6026 1903
Size: 34 3678
Fragments arranged by size:
3678 1769 1312 617 255 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5693 6903 5693
Size: 1210 6591
BclI T'GATC_A
Cuts at: 1548 2539 1548
Size: 991 6810
BfrBI ATG'CAT
Cuts at: 4696 4696
Size: 7801
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6412 136
Size: 107 122 71 631 605 4740 1525
Fragments arranged by size:
4740 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7796 7796
Size: 7801
BlpI GC'TnA_GC
Cuts at: 5218 5218
Size: 7801
Bme1580I G_kGCm'C
Cuts at: 1790 3124 3468 3474 3645 4943 5862 7018 1790
Size: 1334 344 6 171 1298 919 1156 2573
Fragments arranged by size:
2573 1334 1298 1156 919 344 171 6
BmgBI CAC'GTC
Cuts at: 1318 2077 4242 4349 1318
Size: 759 2165 107 4770
Fragments arranged by size:
4770 2165 759 107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2307 2458 2707
Size: 416 53 83 399 24 890 151 249
Cuts at: 2707 5532 6490 442
Size: 2825 958 1753
Fragments arranged by size:
2825 1753 958 890 416 399 249 151
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7801
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4706
Size: 7801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2987 3297 3631 4775 6461 1239
Size: 854 894 310 334 1144 1686 2579
Fragments arranged by size:
2579 1686 1144 894 854 334 310
Bpu10I CC'TnA_GC
Cuts at: 4424 4424
Size: 7801
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3233 5926 6791 7089 3233
Size: 2693 865 298 3945
Fragments arranged by size:
3945 2693 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6464 882
Size: 617 4965 2219
Fragments arranged by size:
4965 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5098 5592 493
Size: 711 3894 494 2702
Fragments arranged by size:
3894 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4791 7795 4791
Size: 3004 4797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3486 5608 5990 6625
Size: 53 83 186 936 1953 2122 382 635
Cuts at: 6625 275
Size: 1451
Fragments arranged by size:
2122 1953 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4839 6234 6632 6906 7053 7464 4839
Size: 1395 398 274 147 411 5176
Fragments arranged by size:
5176 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3073 3103 1178
Size: 30 165 30 1670 30 5876
Fragments arranged by size:
5876 1670 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4230 1186
Size: 3044 4757
BseYI C'CCAG_C
Cuts at: 7004 7500 7004
Size: 496 7305
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3242 1163
Size: 573 1506 5722
Fragments arranged by size:
5722 1506 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5251 6012 7040 1540
Size: 275 186 3250 761 1028 2301
Fragments arranged by size:
3250 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 3468 3755 5862 5947 7018 2088
Size: 1380 287 2107 85 1071 2871
Fragments arranged by size:
2871 2107 1380 1071 287 85
BsmI GAATG_Cn'
Cuts at: 5312 5405 5312
Size: 93 7708
BsmBI CGTCTCn'nnnn_
Cuts at: 3400 3400
Size: 7801
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 3124 3468 3474 3645 3755 4943
Size: 399 298 1036 344 6 171 110 1188
Cuts at: 4943 5120 5862 5947 7018 1391
Size: 177 742 85 1071 2174
Fragments arranged by size:
2174 1188 1071 1036 742 399 344 298
177 171 110 85 6
BspEI T'CCGG_A
Cuts at: 7464 7464
Size: 7801
BspHI T'CATG_A
Cuts at: 3946 4725 4884 5690 7420 3946
Size: 779 159 806 1730 4327
Fragments arranged by size:
4327 1730 806 779 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3336 3447 3744 3767 5156 844
Size: 2492 111 297 23 1389 3489
Fragments arranged by size:
3489 2492 1389 297 111 23
BsrBI CCG'CTC
Cuts at: 1394 1823 5688 1394
Size: 429 3865 3507
Fragments arranged by size:
3865 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3912 6296 6470 65
Size: 1183 2664 2384 174 1396
Fragments arranged by size:
2664 2384 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 4839 6445 6632 1700
Size: 110 292 2737 1606 187 2869
Fragments arranged by size:
2869 2737 1606 292 187 110
BsrGI T'GTAC_A
Cuts at: 96 2772 3126 3342 3820 96
Size: 2676 354 216 478 4077
Fragments arranged by size:
4077 2676 478 354 216
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7801
BssSI C'ACGA_G
Cuts at: 4004 5861 6873 4004
Size: 1857 1012 4932
Fragments arranged by size:
4932 1857 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3759 794
Size: 2965 4836
BstBI TT'CG_AA
Cuts at: 2007 6605 2007
Size: 4598 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7801
BstYI r'GATC_y
Cuts at: 1972 2623 4085 5036 5131 5498 5883 5900 7352
Size: 651 1462 951 95 367 385 17 1452
Cuts at: 7352 7438 7450 7796 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1462 1452 951 651 385 367 346
95 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2042 3663 4439 4446 513
Size: 552 957 20 1621 776 7 3868
Fragments arranged by size:
3868 1621 957 776 552 20 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4649 487
Size: 683 56 3423 3639
Fragments arranged by size:
3639 3423 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 3637 3746 3953 5317 6111 6131 2785
Size: 852 109 207 1364 794 20 4455
Fragments arranged by size:
4455 1364 852 794 207 109 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3198 3233 4499 4534 529
Size: 35 2634 35 1266 35 3796
Fragments arranged by size:
3796 2634 1266 35 35 35
DraI TTT'AAA
Cuts at: 4984 5016 5105 4984
Size: 32 89 7680
Fragments arranged by size:
7680 89 32
DraIII CAC_nnn'GTG
Cuts at: 3265 3265
Size: 7801
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6808 809
Size: 507 5492 1802
Fragments arranged by size:
5492 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2983 5248 6137
Size: 54 1425 50 275 18 1153 2265 889
Cuts at: 6137 7766 8
Size: 1629 43
Fragments arranged by size:
2265 1629 1425 1153 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5248 1537
Size: 275 3436 4090
Fragments arranged by size:
4090 3436 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2523 5731 1353
Size: 917 253 3208 3423
Fragments arranged by size:
3423 3208 917 253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6388 6762 6908 387
Size: 1064 138 4799 374 146 1280
Fragments arranged by size:
4799 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2086 2086
Size: 7801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2149 2538 2987 3105 3297 3631
Size: 393 461 56 389 449 118 192 334
Cuts at: 3631 3710 3749 4356 4775 5864 6461 7248 7516
Size: 79 39 607 419 1089 597 787 268
Cuts at: 7516 1239
Size: 1524
Fragments arranged by size:
1524 1089 787 607 597 461 449 419
393 389 334 268 192 118 79 56
39
EcoNI CCTnn'n_nnAGG
Cuts at: 3344 3649 3344
Size: 305 7496
EcoO109I rG'GnC_Cy
Cuts at: 2706 3121 3641 3642 4435 2706
Size: 415 520 1 793 6072
Fragments arranged by size:
6072 793 520 415 1
EcoRI G'AATT_C
Cuts at: 2571 5110 5488 2571
Size: 2539 378 4884
Fragments arranged by size:
4884 2539 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3173 4514 4613 4882 3173
Size: 1341 99 269 6092
Fragments arranged by size:
6092 1341 269 99
FspI TGC'GCA
Cuts at: 6307 6307
Size: 7801
HaeII r_GCGC'y
Cuts at: 1678 3489 6948 1678
Size: 1811 3459 2531
Fragments arranged by size:
3459 2531 1811
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4028 4060 4077 4109 4845
Size: 32 135 32 2456 32 17 32 736
Cuts at: 4845 4877 6442 6474 6516 6548 1373
Size: 32 1565 32 42 32 2626
Fragments arranged by size:
2626 2456 1565 736 135 42 32 32
32 32 32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 2191 3081 4375 4827 5145 5391
Size: 988 281 253 890 1294 452 318 246
Cuts at: 5391 669
Size: 3079
Fragments arranged by size:
3079 1294 988 890 452 318 281 253
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7801
HpaI GTT'AAC
Cuts at: 5391 5391
Size: 7801
KasI G'GCGC_C
Cuts at: 3485 3485
Size: 7801
KpnI G_GTAC'C
Cuts at: 5126 5126
Size: 7801
MfeI C'AATT_G
Cuts at: 4796 5400 4796
Size: 604 7197
MluI A'CGCG_T
Cuts at: 7677 7677
Size: 7801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2184 2252 2618 3352 4327 6915 7099
Size: 729 150 68 366 734 975 2588 184
Cuts at: 7099 1305
Size: 2007
Fragments arranged by size:
2588 2007 975 734 729 366 184 150
68
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2985 10
Size: 54 1425 343 1153 4826
Fragments arranged by size:
4826 1425 1153 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4889 5759 6118 6277 518
Size: 648 2499 1224 870 359 159 2042
Fragments arranged by size:
2499 2042 1224 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3724 5217 5894 7042 7287 1606
Size: 2118 1493 677 1148 245 2120
Fragments arranged by size:
2120 2118 1493 1148 677 245
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 7801
NarI GG'CG_CC
Cuts at: 3486 3486
Size: 7801
NcoI C'CATG_G
Cuts at: 513 1065 2022 3663 4439 513
Size: 552 957 1641 776 3875
Fragments arranged by size:
3875 1641 957 776 552
NdeI CA'TA_TG
Cuts at: 387 2980 4995 387
Size: 2593 2015 3193
Fragments arranged by size:
3193 2593 2015
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 7801
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7801
NotI GC'GGCC_GC
Cuts at: 5248 5248
Size: 7801
NsiI A_TGCA'T
Cuts at: 4698 4698
Size: 7801
NspI r_CATG'y
Cuts at: 1132 1229 3049 4062 4672 5159 6704 1132
Size: 97 1820 1013 610 487 1545 2229
Fragments arranged by size:
2229 1820 1545 1013 610 487 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7801
PciI A'CATG_T
Cuts at: 6700 6700
Size: 7801
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7801
PfoI T'CCnGG_A
Cuts at: 2109 3003 2109
Size: 894 6907
PmeI GTTT'AAAC
Cuts at: 5105 5105
Size: 7801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5870 5902 7383 7415 5870
Size: 32 1481 32 6256
Fragments arranged by size:
6256 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4435 4435
Size: 7801
PshAI GACnn'nnGTC
Cuts at: 1613 2487 4842 1613
Size: 874 2355 4572
Fragments arranged by size:
4572 2355 874
PsiI TTA'TAA
Cuts at: 4460 4485 5371 4460
Size: 25 886 6890
Fragments arranged by size:
6890 886 25
PspOMI G'GGCC_C
Cuts at: 3120 3641 3120
Size: 521 7280
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3273 3305 3273
Size: 32 7769
PstI C_TGCA'G
Cuts at: 830 3444 3741 4573 5153 830
Size: 2614 297 832 580 3478
Fragments arranged by size:
3478 2614 832 580 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7801
PvuII CAG'CTG
Cuts at: 3724 3724
Size: 7801
SacI G_AGCT'C
Cuts at: 2088 2088
Size: 7801
SalI G'TCGA_C
Cuts at: 1936 5143 1936
Size: 3207 4594
SbfI CC_TGCA'GG
Cuts at: 5153 5153
Size: 7801
ScaI AGT'ACT
Cuts at: 2693 3274 4567 6049 7761 2693
Size: 581 1293 1482 1712 2733
Fragments arranged by size:
2733 1712 1482 1293 581
SfcI C'TryA_G
Cuts at: 826 1045 3440 3737 4569 4813 4959 5029 5149
Size: 219 2395 297 832 244 146 70 120
Cuts at: 5149 6284 6965 7156 826
Size: 1135 681 191 1471
Fragments arranged by size:
2395 1471 1135 832 681 297 244 219
191 146 120 70
SfoI GGC'GCC
Cuts at: 3487 3487
Size: 7801
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7801
SmaI CCC'GGG
Cuts at: 1872 5128 1872
Size: 3256 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2551 3248 4678 5905 6806 7068
Size: 197 926 608 697 1430 1227 901 262
Cuts at: 7068 820
Size: 1553
Fragments arranged by size:
1553 1430 1227 926 901 697 608 262
197
SnaBI TAC'GTA
Cuts at: 493 5098 5592 493
Size: 4605 494 2702
Fragments arranged by size:
4605 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7801
SphI G_CATG'C
Cuts at: 1132 3049 5159 1132
Size: 1917 2110 3774
Fragments arranged by size:
3774 2110 1917
SspI AAT'ATT
Cuts at: 5 52 2920 3134 5725 5
Size: 47 2868 214 2591 2081
Fragments arranged by size:
2868 2591 2081 214 47
StuI AGG'CCT
Cuts at: 1517 4596 4935 1517
Size: 3079 339 4383
Fragments arranged by size:
4383 3079 339
StyI C'CwwG_G
Cuts at: 513 1065 2022 3663 3885 4439 4782 5240 513
Size: 552 957 1641 222 554 343 458 3074
Fragments arranged by size:
3074 1641 957 554 552 458 343 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2673 3406 5828 5845 5998 6183 6628
Size: 1255 1093 733 2422 17 153 185 445
Cuts at: 6628 325
Size: 1498
Fragments arranged by size:
2422 1498 1255 1093 733 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2691 2772 3126
Size: 274 80 33 51 1443 714 81 354
Cuts at: 3126 3272 3342 3820 4565 4745 6047 7759 96
Size: 146 70 478 745 180 1302 1712 138
Fragments arranged by size:
1712 1443 1302 745 714 478 354 274
180 146 138 81 80 70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7761 7793 7761
Size: 32 7769
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7801
XbaI T'CTAG_A
Cuts at: 4120 5137 4120
Size: 1017 6784
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7801
XmaI C'CCGG_G
Cuts at: 1870 5126 1870
Size: 3256 4545
XmnI GAAnn'nnTTC
Cuts at: 4199 5930 4199
Size: 1731 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5609 276
Size: 53 83 186 936 4075 2468
Fragments arranged by size:
4075 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstXI BstZ17I
Bsu36I EcoRV FseI FspAI NruI PacI PmlI PspXI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI