Restriction map for
pFN21ASDA1882
(Circular) (Six-base) MAPSORT of: pFN21ASDA1882.seq Check: 8521 from: 1 to: 8173
pFN21ASDA1882 8173 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4746 5061 4746
Size: 315 7858
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5983 278
Size: 53 83 186 936 4447 2468
Fragments arranged by size:
4447 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3426 5516 1937
Size: 1489 2090 4594
Fragments arranged by size:
4594 2090 1489
Acc65I G'GTAC_C
Cuts at: 5494 5494
Size: 8173
AclI AA'CG_TT
Cuts at: 1563 6300 6673 1563
Size: 4737 373 3063
Fragments arranged by size:
4737 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2101 2877 3003 3110 4650 5015 6236 7620
Size: 469 776 126 107 1540 365 1221 1384
Cuts at: 7620 7888 1632
Size: 268 1917
Fragments arranged by size:
1917 1540 1384 1221 776 469 365 268
126 107
AfeI AGC'GCT
Cuts at: 3182 3182
Size: 8173
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7976
AflIII A'CryG_T
Cuts at: 1203 2703 3079 3714 5442 7072 8049 1203
Size: 1500 376 635 1728 1630 977 1327
Fragments arranged by size:
1728 1630 1500 1327 977 635 376
AgeI A'CCGG_T
Cuts at: 3613 7004 3613
Size: 3391 4782
AhdI GACnn_n'nnGTC
Cuts at: 3505 6902 3505
Size: 3397 4776
AleI CACnn'nnGTG
Cuts at: 1166 2414 3762 5445 1166
Size: 1248 1348 1683 3894
Fragments arranged by size:
3894 1683 1348 1248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4145 5423 5463 1184
Size: 2961 1278 40 3894
Fragments arranged by size:
3894 2961 1278 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 8141
AlwNI CAG_nnn'CTG
Cuts at: 2068 2780 3387 4038 5916 7488 2068
Size: 712 607 651 1878 1572 2753
Fragments arranged by size:
2753 1878 1572 712 651 607
ApaI G_GGCC'C
Cuts at: 5193 5338 5193
Size: 145 8028
ApaLI G'TGCA_C
Cuts at: 5237 5438 6230 7386 5237
Size: 201 792 1156 6024
Fragments arranged by size:
6024 1156 792 201
ApoI r'AATT_y
Cuts at: 1493 3119 3174 4597 5482 5708 5860 7969 1493
Size: 1626 55 1423 885 226 152 2109 1697
Fragments arranged by size:
2109 1697 1626 1423 885 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 6727 160
Size: 872 5695 1606
Fragments arranged by size:
5695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8173
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 4512 4541 4618 4695 4941 5330
Size: 73 267 2302 29 77 77 246 389
Cuts at: 5330 5498 1870
Size: 168 4545
Fragments arranged by size:
4545 2302 389 267 246 168 77 77
73 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4170 4203 4170
Size: 33 8140
BamHI G'GATC_C
Cuts at: 3352 5503 5870 3352
Size: 2151 367 5655
Fragments arranged by size:
5655 2151 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2622 2909 3616 5062
Size: 325 217 627 409 426 287 707 1446
Cuts at: 5062 5494 6949 618
Size: 432 1455 1842
Fragments arranged by size:
1842 1455 1446 707 627 432 426 409
325 287 217
BanII G_rGCy'C
Cuts at: 1391 2827 3204 3582 5177 5193 5279 5338 5492
Size: 1436 377 378 1595 16 86 59 154
Cuts at: 5492 1391
Size: 4072
Fragments arranged by size:
4072 1595 1436 378 377 154 86 59
16
BbeI G_GCGC'C
Cuts at: 5066 5066
Size: 8173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 2991 4012 4583 4744 928
Size: 971 466 626 1021 571 161 4357
Fragments arranged by size:
4357 1021 971 626 571 466 161
BbvCI CC'TCA_GC
Cuts at: 2052 2052
Size: 8173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4487 4521 6364 6398 1903
Size: 34 2550 34 1843 34 3678
Fragments arranged by size:
3678 2550 1843 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6065 7275 6065
Size: 1210 6963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5133 5456 6784
Size: 107 122 71 631 605 3461 323 1328
Cuts at: 6784 136
Size: 1525
Fragments arranged by size:
3461 1525 1328 631 605 323 122 107
71
BglII A'GATC_T
Cuts at: 2334 4136 8168 2334
Size: 1802 4032 2339
Fragments arranged by size:
4032 2339 1802
BlpI GC'TnA_GC
Cuts at: 5590 5590
Size: 8173
Bme1580I G_kGCm'C
Cuts at: 1790 2887 2914 3621 5193 5241 5338 5442 5452
Size: 1097 27 707 1572 48 97 104 10
Cuts at: 5452 6234 7390 1790
Size: 782 1156 2573
Fragments arranged by size:
2573 1572 1156 1097 782 707 104 97
48 27 10
BmgBI CAC'GTC
Cuts at: 1318 2416 2960 1318
Size: 1098 544 6531
Fragments arranged by size:
6531 1098 544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3947 4345 5427
Size: 416 53 83 399 24 2530 398 1082
Cuts at: 5427 5904 6862 442
Size: 477 958 1753
Fragments arranged by size:
2530 1753 1082 958 477 416 399 398
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3675 1056
Size: 2619 5554
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2841 4294 2841
Size: 1453 6720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 4289 4632 4940 4970 4999 5066 5171
Size: 1280 1770 343 308 30 29 67 105
Cuts at: 5171 5263 5474 6833 1239
Size: 92 211 1359 2579
Fragments arranged by size:
2579 1770 1359 1280 343 308 211 105
92 67 30 29
Bpu10I CC'TnA_GC
Cuts at: 2052 2975 2052
Size: 923 7250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 6298 7163 7461 4342
Size: 1956 865 298 5054
Fragments arranged by size:
5054 1956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6836 882
Size: 617 5337 2219
Fragments arranged by size:
5337 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2323 2647 3080 4573 5443 5470 5964
Size: 711 1119 324 433 1493 870 27 494
Cuts at: 5964 493
Size: 2702
Fragments arranged by size:
2702 1493 1119 870 711 494 433 324
27
BsaBI GATnn'nnATC
Cuts at: 8167 8167
Size: 8173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5063 5980 6362 6997
Size: 53 83 186 936 3530 917 382 635
Cuts at: 6997 275
Size: 1451
Fragments arranged by size:
3530 1451 936 917 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3613 5169 5376 6606 7004 7278 7425 7836 3613
Size: 1556 207 1230 398 274 147 411 3950
Fragments arranged by size:
3950 1556 1230 411 398 274 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3278 3308 3890 3920 5249
Size: 30 165 30 1875 30 582 30 1329
Cuts at: 5249 5279 1178
Size: 30 4072
Fragments arranged by size:
4072 1875 1329 582 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 3305 5330 1186
Size: 934 1185 2025 4029
Fragments arranged by size:
4029 2025 1185 934
BseYI C'CCAG_C
Cuts at: 2169 3387 3703 4711 4966 7376 7872 2169
Size: 1218 316 1008 255 2410 496 2470
Fragments arranged by size:
2470 2410 1218 1008 496 316 255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 4125 4796 5237 5324 1163
Size: 573 609 1780 671 441 87 4012
Fragments arranged by size:
4012 1780 671 609 573 441 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5623 6384 7412 1540
Size: 275 186 3622 761 1028 2301
Fragments arranged by size:
3622 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2827 3204 5052 5241 5442 6234 6319 7390 2827
Size: 377 1848 189 201 792 85 1071 3610
Fragments arranged by size:
3610 1848 1071 792 377 201 189 85
BsmI GAATG_Cn'
Cuts at: 3043 3136 5684 5777 3043
Size: 93 2548 93 5439
Fragments arranged by size:
5439 2548 93 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2104 5269 2104
Size: 3165 5008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2827 2887 2914 3204 3582 3621 5052
Size: 399 1037 60 27 290 378 39 1431
Cuts at: 5052 5177 5193 5241 5279 5338 5442 5452 5492
Size: 125 16 48 38 59 104 10 40
Cuts at: 5492 6234 6319 7390 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1431 1071 1037 742 399 378 290
125 104 85 60 59 48 40 39
38 27 16 10
BspEI T'CCGG_A
Cuts at: 7836 7836
Size: 8173
BspHI T'CATG_A
Cuts at: 6062 7792 6062
Size: 1730 6443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4062 4489 4746 5061 5259 5528 844
Size: 3218 427 257 315 198 269 3489
Fragments arranged by size:
3489 3218 427 315 269 257 198
BsrBI CCG'CTC
Cuts at: 1394 1823 3775 4930 6060 1394
Size: 429 1952 1155 1130 3507
Fragments arranged by size:
3507 1952 1155 1130 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 6668 6842 65
Size: 1183 2900 2520 174 1396
Fragments arranged by size:
2900 2520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2874 3613 4817 6817 7004 1700
Size: 110 1064 739 1204 2000 187 2869
Fragments arranged by size:
2869 2000 1204 1064 739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8173
BssSI C'ACGA_G
Cuts at: 2072 5384 6233 7245 2072
Size: 3312 849 1012 3000
Fragments arranged by size:
3312 3000 1012 849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8173
BstBI TT'CG_AA
Cuts at: 2007 6977 2007
Size: 4970 3203
BstEII G'GTnAC_C
Cuts at: 1184 4004 4273 1184
Size: 2820 269 5084
Fragments arranged by size:
5084 2820 269
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 3895 4918 5418 2512
Size: 1383 1023 500 5267
Fragments arranged by size:
5267 1383 1023 500
BstYI r'GATC_y
Cuts at: 1972 2121 2334 2544 3352 4136 4441 4953 5503
Size: 149 213 210 808 784 305 512 550
Cuts at: 5503 5870 6255 6272 7724 7810 7822 8168 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 808 784 550 512 385 367
346 305 213 210 149 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3573 3763 4014 513
Size: 552 2508 190 251 4672
Fragments arranged by size:
4672 2508 552 251 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3209 3265 3568 487
Size: 683 56 1983 56 303 5092
Fragments arranged by size:
5092 1983 683 303 56 56
BtsI GCAGTG_nn'
Cuts at: 2051 3269 4736 5008 5078 5689 6483 6503 2051
Size: 1218 1467 272 70 611 794 20 3721
Fragments arranged by size:
3721 1467 1218 794 611 272 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8138
DraI TTT'AAA
Cuts at: 5477 5477
Size: 8173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7180 809
Size: 507 5864 1802
Fragments arranged by size:
5864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2297 2876
Size: 54 1425 50 275 18 456 11 579
Cuts at: 2876 3252 3680 4377 5314 5620 6509 8138 8
Size: 376 428 697 937 306 889 1629 43
Fragments arranged by size:
1629 1425 937 889 697 579 456 428
376 306 275 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 5620 1537
Size: 275 3808 4090
Fragments arranged by size:
4090 3808 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2453 2810 3162 3765 3933 4920 5348 6103
Size: 1100 357 352 603 168 987 428 755
Cuts at: 6103 1353
Size: 3423
Fragments arranged by size:
3423 1100 987 755 603 428 357 352
168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6760 7134 7280 387
Size: 1064 138 5171 374 146 1280
Fragments arranged by size:
5171 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2825 3202 2825
Size: 377 7796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2519 2877 3003 3110 4289 4632
Size: 393 469 418 358 126 107 1179 343
Cuts at: 4632 4650 4940 4970 4999 5015 5066 5171 5263
Size: 18 290 30 29 16 51 105 92
Cuts at: 5263 5474 6236 6833 7620 7888 1239
Size: 211 762 597 787 268 1524
Fragments arranged by size:
1524 1179 787 762 597 469 418 393
358 343 290 268 211 126 107 105
92 51 30 29 18 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3436 3436
Size: 8173
EcoO109I rG'GnC_Cy
Cuts at: 2653 3300 3970 5189 5190 5334 5335 2653
Size: 647 670 1219 1 144 1 5491
Fragments arranged by size:
5491 1219 670 647 144 1 1
EcoRI G'AATT_C
Cuts at: 5482 5860 5482
Size: 378 7795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4876 4876
Size: 8173
FspI TGC'GCA
Cuts at: 6679 6679
Size: 8173
HaeII r_GCGC'y
Cuts at: 1678 3184 3923 5066 7320 1678
Size: 1506 739 1143 2254 2531
Fragments arranged by size:
2531 2254 1506 1143 739
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 3491 3523 3890
Size: 32 135 32 695 32 1192 32 367
Cuts at: 3890 3922 4101 4133 4160 4192 5249 5281 6814
Size: 32 179 32 27 32 1057 32 1533
Cuts at: 6814 6846 6888 6920 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1533 1192 1057 695 367 179 135
42 32 32 32 32 32 32 32
32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 3720 5517 5763 669
Size: 988 281 1782 1797 246 3079
Fragments arranged by size:
3079 1797 1782 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3629 3697 748
Size: 2881 68 5224
Fragments arranged by size:
5224 2881 68
HpaI GTT'AAC
Cuts at: 5763 5763
Size: 8173
KasI G'GCGC_C
Cuts at: 5062 5062
Size: 8173
KpnI G_GTAC'C
Cuts at: 5498 5498
Size: 8173
MfeI C'AATT_G
Cuts at: 5772 5772
Size: 8173
MluI A'CGCG_T
Cuts at: 8049 8049
Size: 8173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3489 7287 7471 1305
Size: 2184 3798 184 2007
Fragments arranged by size:
3798 2184 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2299 4379 10
Size: 54 1425 343 467 2080 3804
Fragments arranged by size:
3804 2080 1425 467 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 2708 3762 4400 5445 6131 6490
Size: 648 1248 294 1054 638 1045 686 359
Cuts at: 6490 6649 518
Size: 159 2042
Fragments arranged by size:
2042 1248 1054 1045 686 648 638 359
294 159
MspA1I CmG'CkG
Cuts at: 1606 3829 3891 4918 5059 5589 6266 7414 7659
Size: 2223 62 1027 141 530 677 1148 245
Cuts at: 7659 1606
Size: 2120
Fragments arranged by size:
2223 2120 1148 1027 677 530 245 141
62
NaeI GCC'GGC
Cuts at: 4819 4819
Size: 8173
NarI GG'CG_CC
Cuts at: 5063 5063
Size: 8173
NcoI C'CATG_G
Cuts at: 513 1065 3573 513
Size: 552 2508 5113
Fragments arranged by size:
5113 2508 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8173
NgoMIV G'CCGG_C
Cuts at: 4817 4817
Size: 8173
NheI G'CTAG_C
Cuts at: 1052 3671 1052
Size: 2619 5554
NotI GC'GGCC_GC
Cuts at: 5620 5620
Size: 8173
NspI r_CATG'y
Cuts at: 1132 1229 2044 2638 2707 3650 4667 5531 7076
Size: 97 815 594 69 943 1017 864 1545
Cuts at: 7076 1132
Size: 2229
Fragments arranged by size:
2229 1545 1017 943 864 815 594 97
69
PasI CC'CwG_GG
Cuts at: 1348 4654 4883 1348
Size: 3306 229 4638
Fragments arranged by size:
4638 3306 229
PciI A'CATG_T
Cuts at: 2703 7072 2703
Size: 4369 3804
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 2295 2452 3421 4834 1108
Size: 981 206 157 969 1413 4447
Fragments arranged by size:
4447 1413 981 969 206 157
PfoI T'CCnGG_A
Cuts at: 3318 4743 4872 5043 5217 3318
Size: 1425 129 171 174 6274
Fragments arranged by size:
6274 1425 174 171 129
PmeI GTTT'AAAC
Cuts at: 5477 5477
Size: 8173
PmlI CAC'GTG
Cuts at: 2323 2647 4573 5443 2323
Size: 324 1926 870 5053
Fragments arranged by size:
5053 1926 870 324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2471 2503 6242 6274 7755 7787 2471
Size: 32 3739 32 1481 32 2857
Fragments arranged by size:
3739 2857 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2653 3970 2653
Size: 1317 6856
PshAI GACnn'nnGTC
Cuts at: 1613 3472 3962 1613
Size: 1859 490 5824
Fragments arranged by size:
5824 1859 490
PsiI TTA'TAA
Cuts at: 5743 5743
Size: 8173
PspOMI G'GGCC_C
Cuts at: 5189 5334 5189
Size: 145 8028
PstI C_TGCA'G
Cuts at: 830 2385 2667 2682 4116 4440 5058 5073 5525
Size: 1555 282 15 1434 324 618 15 452
Cuts at: 5525 830
Size: 3478
Fragments arranged by size:
3478 1555 1434 618 452 324 282 15
15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8173
PvuII CAG'CTG
Cuts at: 3829 4918 5059 3829
Size: 1089 141 6943
Fragments arranged by size:
6943 1089 141
SacI G_AGCT'C
Cuts at: 2827 3204 2827
Size: 377 7796
SalI G'TCGA_C
Cuts at: 1936 5515 1936
Size: 3579 4594
SanDI GG'GwC_CC
Cuts at: 3970 3970
Size: 8173
SapI GCTCTTCn'nnn_
Cuts at: 3765 4920 3765
Size: 1155 7018
SbfI CC_TGCA'GG
Cuts at: 2667 4440 5525 2667
Size: 1773 1085 5315
Fragments arranged by size:
5315 1773 1085
ScaI AGT'ACT
Cuts at: 2947 6421 8133 2947
Size: 3474 1712 2987
Fragments arranged by size:
3474 2987 1712
SfcI C'TryA_G
Cuts at: 826 1045 2191 2381 2663 2678 3184 3337 3485
Size: 219 1146 190 282 15 506 153 148
Cuts at: 3485 4112 4436 4564 5054 5069 5521 6656 7337
Size: 627 324 128 490 15 452 1135 681
Cuts at: 7337 7528 826
Size: 191 1471
Fragments arranged by size:
1471 1146 1135 681 627 506 490 452
324 282 219 191 190 153 148 128
15 15
SfoI GGC'GCC
Cuts at: 5064 5064
Size: 8173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8173
SgrAI Cr'CCGG_yG
Cuts at: 1700 3613 4817 1700
Size: 1913 1204 5056
Fragments arranged by size:
5056 1913 1204
SmaI CCC'GGG
Cuts at: 1872 4620 5500 1872
Size: 2748 880 4545
Fragments arranged by size:
4545 2748 880
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 6277 7178 7440 820
Size: 197 926 2378 1956 901 262 1553
Fragments arranged by size:
2378 1956 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5470 5964 493
Size: 4977 494 2702
Fragments arranged by size:
4977 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8173
SphI G_CATG'C
Cuts at: 1132 2638 5531 1132
Size: 1506 2893 3774
Fragments arranged by size:
3774 2893 1506
SrfI GCCC'GGGC
Cuts at: 4620 4620
Size: 8173
SspI AAT'ATT
Cuts at: 5 52 4243 6097 5
Size: 47 4191 1854 2081
Fragments arranged by size:
4191 2081 1854 47
StuI AGG'CCT
Cuts at: 1517 4947 5465 1517
Size: 3430 518 4225
Fragments arranged by size:
4225 3430 518
StyI C'CwwG_G
Cuts at: 513 1065 2289 2626 3573 5612 513
Size: 552 1224 337 947 2039 3074
Fragments arranged by size:
3074 2039 1224 947 552 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3282 6200 6217 6370 6555 7000 325
Size: 1255 1702 2918 17 153 185 445 1498
Fragments arranged by size:
2918 1702 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2945 6419 8131
Size: 274 80 33 51 1443 968 3474 1712
Cuts at: 8131 96
Size: 138
Fragments arranged by size:
3474 1712 1443 968 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2753 2785 3278 3310 3616 3648 4449 4481 8133
Size: 32 493 32 306 32 801 32 3652
Cuts at: 8133 8165 2753
Size: 32 2761
Fragments arranged by size:
3652 2761 801 493 306 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5261 1316
Size: 3945 4228
XbaI T'CTAG_A
Cuts at: 5509 5509
Size: 8173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3265 4202 3265
Size: 937 7236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8173
XmaI C'CCGG_G
Cuts at: 1870 4618 5498 1870
Size: 2748 880 4545
Fragments arranged by size:
4545 2748 880
XmnI GAAnn'nnTTC
Cuts at: 2114 3123 4092 6302 2114
Size: 1009 969 2210 3985
Fragments arranged by size:
3985 2210 1009 969
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5981 276
Size: 53 83 186 936 4447 2468
Fragments arranged by size:
4447 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SexAI SfiI SwaI