Restriction map for
pFN21ASDA1872
(Circular) (Six-base) MAPSORT of: pFN21ASDA1872.seq Check: 3060 from: 1 to: 5842
pFN21ASDA1872 5842 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3652 278
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2792 2837 3026 3081 3905 5289 5557 1632
Size: 1160 45 189 55 824 1384 268 1917
Fragments arranged by size:
1917 1384 1160 824 268 189 55 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 2959 4741 5718 1203
Size: 1756 1782 977 1327
Fragments arranged by size:
1782 1756 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 2390 1166
Size: 1224 4618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5842
AlwNI CAG_nnn'CTG
Cuts at: 3585 5157 3585
Size: 1572 4270
ApaLI G'TGCA_C
Cuts at: 2918 3899 5055 2918
Size: 981 1156 3705
Fragments arranged by size:
3705 1156 981
ApoI r'AATT_y
Cuts at: 1493 2176 3151 3377 3529 5638 1493
Size: 683 975 226 152 2109 1697
Fragments arranged by size:
2109 1697 975 683 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 3167 1870
Size: 73 471 753 4545
Fragments arranged by size:
4545 753 471 73
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2475 2732 3079 3163 4618
Size: 325 217 627 688 257 347 84 1455
Cuts at: 4618 618
Size: 1842
Fragments arranged by size:
1842 1455 688 627 347 325 257 217
84
BanII G_rGCy'C
Cuts at: 1391 2694 3161 1391
Size: 1303 467 4072
Fragments arranged by size:
4072 1303 467
BbeI G_GCGC'C
Cuts at: 2479 2479
Size: 5842
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 2210 2385 2593 2937 928
Size: 971 255 56 175 208 344 3833
Fragments arranged by size:
3833 971 344 255 208 175 56
BbvCI CC'TCA_GC
Cuts at: 2703 2703
Size: 5842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4033 4067 1903
Size: 34 2096 34 3678
Fragments arranged by size:
3678 2096 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 3734 4944 2272
Size: 1462 1210 3170
Fragments arranged by size:
3170 1462 1210
BclI T'GATC_A
Cuts at: 1548 2493 2826 1548
Size: 945 333 4564
Fragments arranged by size:
4564 945 333
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4453 136
Size: 107 122 71 631 605 2781 1525
Fragments arranged by size:
2781 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2089 5837 2089
Size: 3748 2094
BlpI GC'TnA_GC
Cuts at: 3259 3259
Size: 5842
Bme1580I G_kGCm'C
Cuts at: 1790 2922 3903 5059 1790
Size: 1132 981 1156 2573
Fragments arranged by size:
2573 1156 1132 981
BmgBI CAC'GTC
Cuts at: 1318 2065 2962 1318
Size: 747 897 4198
Fragments arranged by size:
4198 897 747
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3573 4531 442
Size: 416 53 83 399 24 2156 958 1753
Fragments arranged by size:
2156 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2390 2957 4502 1239
Size: 1124 27 567 1545 2579
Fragments arranged by size:
2579 1545 1124 567 27
Bpu10I CC'TnA_GC
Cuts at: 2703 2703
Size: 5842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 2324 3112 4505 882
Size: 617 813 12 788 1393 2219
Fragments arranged by size:
2219 1393 813 788 617 12
BsaAI yAC'GTr
Cuts at: 493 1204 2917 3139 3633 493
Size: 711 1713 222 494 2702
Fragments arranged by size:
2702 1713 711 494 222
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2476 3649 4031 4666
Size: 53 83 186 936 943 1173 382 635
Cuts at: 4666 275
Size: 1451
Fragments arranged by size:
1451 1173 943 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2046 2796 4275 4673 4947 5094 5505 2046
Size: 750 1479 398 274 147 411 2383
Fragments arranged by size:
2383 1479 750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2143 2173 1178
Size: 30 165 30 740 30 4847
Fragments arranged by size:
4847 740 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2151 1186
Size: 965 4877
BseYI C'CCAG_C
Cuts at: 3033 5045 5541 3033
Size: 2012 496 3334
Fragments arranged by size:
3334 2012 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 1163
Size: 573 774 4495
Fragments arranged by size:
4495 774 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 3292 4053 5081 1540
Size: 275 186 796 495 761 1028 2301
Fragments arranged by size:
2301 1028 796 761 495 275 186
BsiHKAI G_wGCw'C
Cuts at: 2514 2694 2922 3903 3988 5059 2514
Size: 180 228 981 85 1071 3297
Fragments arranged by size:
3297 1071 981 228 180 85
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
BsmBI CGTCTCn'nnnn_
Cuts at: 2108 2108
Size: 5842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2514 2694 2922 3161 3903 3988 5059
Size: 399 724 180 228 239 742 85 1071
Cuts at: 5059 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 724 399 239 228 180
85
BspEI T'CCGG_A
Cuts at: 2046 5505 2046
Size: 3459 2383
BspHI T'CATG_A
Cuts at: 3731 5461 3731
Size: 1730 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2532 3197 844
Size: 1688 665 3489
Fragments arranged by size:
3489 1688 665
BsrBI CCG'CTC
Cuts at: 1394 1823 3729 1394
Size: 429 1906 3507
Fragments arranged by size:
3507 1906 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 2842 3902 4914 2842
Size: 1060 1012 3770
Fragments arranged by size:
3770 1060 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5842
BstBI TT'CG_AA
Cuts at: 2007 4646 2007
Size: 2639 3203
BstEII G'GTnAC_C
Cuts at: 1184 2554 1184
Size: 1370 4472
BstYI r'GATC_y
Cuts at: 1972 2089 2428 2907 2967 3172 3539 3924 3941
Size: 117 339 479 60 205 367 385 17
Cuts at: 3941 5393 5479 5491 5837 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 479 385 367 346 339 205
117 86 60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2391 2560 513
Size: 552 957 369 169 3795
Fragments arranged by size:
3795 957 552 369 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5103
Fragments arranged by size:
5103 683 56
BtsI GCAGTG_nn'
Cuts at: 3358 4152 4172 3358
Size: 794 20 5028
Fragments arranged by size:
5028 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2865 3289
Size: 54 1425 50 275 18 564 471 424
Cuts at: 3289 4178 5807 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 564 471 424 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 2669 2988 3057 3772 1353
Size: 753 563 319 69 715 3423
Fragments arranged by size:
3423 753 715 563 319 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2500 2761 4429 4803 4949 387
Size: 1064 138 911 261 1668 374 146 1280
Fragments arranged by size:
1668 1280 1064 911 374 261 146 138
EcoICRI GAG'CTC
Cuts at: 2692 2692
Size: 5842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2390 2792 2837 2957 3026 3081
Size: 393 731 27 402 45 120 69 55
Cuts at: 3081 3905 4502 5289 5557 1239
Size: 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 731 597 402 393 268
120 69 55 45 27
EcoO109I rG'GnC_Cy
Cuts at: 2253 3030 2253
Size: 777 5065
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 2466 2479 4989 1678
Size: 788 13 2510 2531
Fragments arranged by size:
2531 2510 788 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 4483 4515 4557
Size: 32 135 32 569 32 2310 32 42
Cuts at: 4557 4589 1373
Size: 32 2626
Fragments arranged by size:
2626 2310 569 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KasI G'GCGC_C
Cuts at: 2475 2475
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 5140 1305
Size: 3651 184 2007
Fragments arranged by size:
3651 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2867 10
Size: 54 1425 343 564 471 2985
Fragments arranged by size:
2985 1425 564 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 2549 2717 2951 3800 4159 4318
Size: 648 1224 159 168 234 849 359 159
Cuts at: 4318 518
Size: 2042
Fragments arranged by size:
2042 1224 849 648 359 234 168 159
159
MspA1I CmG'CkG
Cuts at: 1606 3258 3935 5083 5328 1606
Size: 1652 677 1148 245 2120
Fragments arranged by size:
2120 1652 1148 677 245
NarI GG'CG_CC
Cuts at: 2476 2476
Size: 5842
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4333
Fragments arranged by size:
4333 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2413 3133 3200 4745 1132
Size: 97 1184 720 67 1545 2229
Fragments arranged by size:
2229 1545 1184 720 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 5424 5456 3911
Size: 32 1481 32 4297
Fragments arranged by size:
4297 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2253 2253
Size: 5842
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PstI C_TGCA'G
Cuts at: 830 3194 830
Size: 2364 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
SacI G_AGCT'C
Cuts at: 2694 2694
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SanDI GG'GwC_CC
Cuts at: 2253 2253
Size: 5842
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 4090 5802 4090
Size: 1712 4130
SfcI C'TryA_G
Cuts at: 826 1045 3190 4325 5006 5197 826
Size: 219 2145 1135 681 191 1471
Fragments arranged by size:
2145 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2477 2477
Size: 5842
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 2416 3169 1872
Size: 544 753 4545
Fragments arranged by size:
4545 753 544
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3946 4847 5109 820
Size: 197 926 2003 901 262 1553
Fragments arranged by size:
2003 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3139 3633 493
Size: 2646 494 2702
Fragments arranged by size:
2702 2646 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 2583 1517
Size: 1066 4776
StyI C'CwwG_G
Cuts at: 513 1065 2022 3281 513
Size: 552 957 1259 3074
Fragments arranged by size:
3074 1259 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 2573 3869 3886 4039 4224 4669
Size: 1255 768 225 1296 17 153 185 445
Cuts at: 4669 325
Size: 1498
Fragments arranged by size:
1498 1296 1255 768 445 225 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4088 5800 96
Size: 274 80 33 51 1443 2111 1712 138
Fragments arranged by size:
2111 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 5802 5834 2143
Size: 32 3627 32 2151
Fragments arranged by size:
3627 2151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2978 1316
Size: 1662 4180
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5842
XmaI C'CCGG_G
Cuts at: 1870 2414 3167 1870
Size: 544 753 4545
Fragments arranged by size:
4545 753 544
XmnI GAAnn'nnTTC
Cuts at: 2091 2663 3971 2091
Size: 572 1308 3962
Fragments arranged by size:
3962 1308 572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3650 276
Size: 53 83 186 936 2116 2468
Fragments arranged by size:
2468 2116 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI
SexAI SfiI SrfI SwaI