Restriction map for
pFN21ASDA1860
(Circular) (Six-base) MAPSORT of: pFN21ASDA1860.seq Check: 8084 from: 1 to: 6793
pFN21ASDA1860 6793 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4603 278
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4136 1937
Size: 2199 4594
Acc65I G'GTAC_C
Cuts at: 4114 4114
Size: 6793
AclI AA'CG_TT
Cuts at: 1563 4920 5293 1563
Size: 3357 373 3063
Fragments arranged by size:
3357 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 2611 3383 4856 6240 6508 1632
Size: 956 23 772 1473 1384 268 1917
Fragments arranged by size:
1917 1473 1384 956 772 268 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6596
AflIII A'CryG_T
Cuts at: 1203 2661 2856 5692 6669 1203
Size: 1458 195 2836 977 1327
Fragments arranged by size:
2836 1458 1327 977 195
AgeI A'CCGG_T
Cuts at: 5624 5624
Size: 6793
AhdI GACnn_n'nnGTC
Cuts at: 4015 5522 4015
Size: 1507 5286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6793
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6793
AlwNI CAG_nnn'CTG
Cuts at: 2329 4536 6108 2329
Size: 2207 1572 3014
Fragments arranged by size:
3014 2207 1572
ApaI G_GGCC'C
Cuts at: 3795 3795
Size: 6793
ApaLI G'TGCA_C
Cuts at: 2528 4850 6006 2528
Size: 2322 1156 3315
Fragments arranged by size:
3315 2322 1156
ApoI r'AATT_y
Cuts at: 1493 4102 4328 4480 6589 1493
Size: 2609 226 152 2109 1697
Fragments arranged by size:
2609 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5347 160
Size: 872 4315 1606
Fragments arranged by size:
4315 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2171 2496 2924 2996 3574 4118
Size: 73 104 124 325 428 72 578 544
Cuts at: 4118 1870
Size: 4545
Fragments arranged by size:
4545 578 544 428 325 124 104 73
72
BamHI G'GATC_C
Cuts at: 3968 4123 4490 3968
Size: 155 367 6271
Fragments arranged by size:
6271 367 155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2138 3155 3187 3227
Size: 325 217 627 291 60 1017 32 40
Cuts at: 3227 3626 3637 3670 3950 4114 5569 618
Size: 399 11 33 280 164 1455 1842
Fragments arranged by size:
1842 1455 1017 627 399 325 291 280
217 164 60 40 33 32 11
BanII G_rGCy'C
Cuts at: 1391 2678 2997 3795 4112 1391
Size: 1287 319 798 317 4072
Fragments arranged by size:
4072 1287 798 319 317
BbeI G_GCGC'C
Cuts at: 3159 3191 3954 3159
Size: 32 763 5998
Fragments arranged by size:
5998 763 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 2414 3137 928
Size: 971 120 395 723 4584
Fragments arranged by size:
4584 971 723 395 120
BbvCI CC'TCA_GC
Cuts at: 2207 2207
Size: 6793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4984 5018 1903
Size: 34 3047 34 3678
Fragments arranged by size:
3678 3047 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4685 5895 4685
Size: 1210 5583
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6793
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3821 3841 5404
Size: 107 122 71 631 605 2149 20 1563
Cuts at: 5404 136
Size: 1525
Fragments arranged by size:
2149 1563 1525 631 605 122 107 71
20
BglII A'GATC_T
Cuts at: 6788 6788
Size: 6793
BlpI GC'TnA_GC
Cuts at: 3582 4210 3582
Size: 628 6165
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2532 2799 3642 3795 4854 6010 1790
Size: 353 389 267 843 153 1059 1156 2573
Fragments arranged by size:
2573 1156 1059 843 389 353 267 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6793
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2144 4524 5482
Size: 416 53 83 399 24 727 2380 958
Cuts at: 5482 442
Size: 1753
Fragments arranged by size:
2380 1753 958 727 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 2761 3421 3640 4101 5453 1239
Size: 1224 298 660 219 461 1352 2579
Fragments arranged by size:
2579 1352 1224 660 461 298 219
Bpu10I CC'TnA_GC
Cuts at: 2207 3622 2207
Size: 1415 5378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 4918 5783 6081 2789
Size: 2129 865 298 3501
Fragments arranged by size:
3501 2129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5456 882
Size: 617 3957 2219
Fragments arranged by size:
3957 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2188 4090 4584 493
Size: 711 984 1902 494 2702
Fragments arranged by size:
2702 1902 984 711 494
BsaBI GATnn'nnATC
Cuts at: 2954 6787 2954
Size: 3833 2960
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 3188 3951 4600
Size: 53 83 186 936 1623 32 763 649
Cuts at: 4600 4982 5617 275
Size: 382 635 1451
Fragments arranged by size:
1623 1451 936 763 649 635 382 186
83 53 32
BsaWI w'CCGG_w
Cuts at: 5226 5624 5898 6045 6456 5226
Size: 398 274 147 411 5563
Fragments arranged by size:
5563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3403 3433 4074 4104 1178
Size: 30 165 30 2000 30 641 30 3867
Fragments arranged by size:
3867 2000 641 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 2189 2987 3014 3152 3383 1186
Size: 903 100 798 27 138 231 4596
Fragments arranged by size:
4596 903 798 231 138 100 27
BseYI C'CCAG_C
Cuts at: 2301 2312 2644 2905 3121 3191 5996 6492 2301
Size: 11 332 261 216 70 2805 496 2602
Fragments arranged by size:
2805 2602 496 332 261 216 70 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3297 3305 3803 3810 1163
Size: 573 1561 8 498 7 4146
Fragments arranged by size:
4146 1561 573 498 8 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 4243 5004 6032 1540
Size: 275 186 58 2184 761 1028 2301
Fragments arranged by size:
2301 2184 1028 761 275 186 58
BsiHKAI G_wGCw'C
Cuts at: 2532 3347 3512 4854 4939 6010 2532
Size: 815 165 1342 85 1071 3315
Fragments arranged by size:
3315 1342 1071 815 165 85
BsmI GAATG_Cn'
Cuts at: 4304 4397 4304
Size: 93 6700
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2532 2678 2799 2997 3347 3512
Size: 399 353 389 146 121 198 350 165
Cuts at: 3512 3642 3795 4112 4854 4939 6010 1391
Size: 130 153 317 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 389 353 350 317
198 165 153 146 130 121 85
BspEI T'CCGG_A
Cuts at: 6456 6456
Size: 6793
BspHI T'CATG_A
Cuts at: 2349 2934 4682 6412 2349
Size: 585 1748 1730 2730
Fragments arranged by size:
2730 1748 1730 585
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4148 844
Size: 3304 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 2130 3373 3490 3688 4680 1394
Size: 429 274 33 1243 117 198 992 3507
Fragments arranged by size:
3507 1243 992 429 274 198 117 33
BsrDI GCAATG_nn'
Cuts at: 65 1248 5288 5462 65
Size: 1183 4040 174 1396
Fragments arranged by size:
4040 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3750 5437 5624 1700
Size: 110 1940 1687 187 2869
Fragments arranged by size:
2869 1940 1687 187 110
BsrGI T'GTAC_A
Cuts at: 96 2554 2760 96
Size: 2458 206 4129
Fragments arranged by size:
4129 2458 206
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6793
BssSI C'ACGA_G
Cuts at: 2462 3505 4853 5865 2462
Size: 1043 1348 1012 3390
Fragments arranged by size:
3390 1348 1043 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6793
BstBI TT'CG_AA
Cuts at: 2007 5597 2007
Size: 3590 3203
BstEII G'GTnAC_C
Cuts at: 1184 3901 1184
Size: 2717 4076
BstXI CCAn_nnnn'nTGG
Cuts at: 3445 3445
Size: 6793
BstYI r'GATC_y
Cuts at: 1972 3480 3968 4123 4490 4875 4892 6344 6430
Size: 1508 488 155 367 385 17 1452 86
Cuts at: 6430 6442 6788 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1508 1452 488 385 367 346 155
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4013 4013
Size: 6793
BtgI C'CryG_G
Cuts at: 513 1065 3405 3444 3632 3730 513
Size: 552 2340 39 188 98 3576
Fragments arranged by size:
3576 2340 552 188 98 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 487
Size: 683 56 917 5137
Fragments arranged by size:
5137 917 683 56
BtsI GCAGTG_nn'
Cuts at: 2095 4309 5103 5123 2095
Size: 2214 794 20 3765
Fragments arranged by size:
3765 2214 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6793
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6758
DraI TTT'AAA
Cuts at: 4097 4097
Size: 6793
DraIII CAC_nnn'GTG
Cuts at: 2138 2138
Size: 6793
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5800 809
Size: 507 4484 1802
Fragments arranged by size:
4484 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2473 3736
Size: 54 1425 50 275 18 262 381 1263
Cuts at: 3736 4240 5129 6758 8
Size: 504 889 1629 43
Fragments arranged by size:
1629 1425 1263 889 504 381 275 262
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4240 1537
Size: 275 2428 4090
Fragments arranged by size:
4090 2428 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 3872 4723 1353
Size: 1912 607 851 3423
Fragments arranged by size:
3423 1912 851 607
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3371 3508 5380 5754 5900 387
Size: 1064 138 1782 137 1872 374 146 1280
Fragments arranged by size:
1872 1782 1280 1064 374 146 138 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2588 2611 2761 3383 3421 3640
Size: 393 831 125 23 150 622 38 219
Cuts at: 3640 4101 4856 5453 6240 6508 1239
Size: 461 755 597 787 268 1524
Fragments arranged by size:
1524 831 787 755 622 597 461 393
268 219 150 125 38 23
EcoO109I rG'GnC_Cy
Cuts at: 2356 2563 2845 3792 4009 2356
Size: 207 282 947 217 5140
Fragments arranged by size:
5140 947 282 217 207
EcoRI G'AATT_C
Cuts at: 4102 4480 4102
Size: 378 6415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 6793
FspI TGC'GCA
Cuts at: 5299 5299
Size: 6793
HaeII r_GCGC'y
Cuts at: 1678 3159 3191 3459 3589 3954 5940 1678
Size: 1481 32 268 130 365 1986 2531
Fragments arranged by size:
2531 1986 1481 365 268 130 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 3403 3435 3575
Size: 32 135 32 476 32 1323 32 140
Cuts at: 3575 3607 3960 3992 4001 4033 5434 5466 5508
Size: 32 353 32 9 32 1401 32 42
Cuts at: 5508 5540 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 1323 476 353 140 135 42
32 32 32 32 32 32 32 32
32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 4137 4383 669
Size: 988 281 924 1275 246 3079
Fragments arranged by size:
3079 1275 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6793
HpaI GTT'AAC
Cuts at: 4383 4383
Size: 6793
KasI G'GCGC_C
Cuts at: 3155 3187 3950 3155
Size: 32 763 5998
Fragments arranged by size:
5998 763 32
KpnI G_GTAC'C
Cuts at: 4118 4118
Size: 6793
MfeI C'AATT_G
Cuts at: 4392 4392
Size: 6793
MluI A'CGCG_T
Cuts at: 6669 6669
Size: 6793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2533 5907 6091 1305
Size: 1228 3374 184 2007
Fragments arranged by size:
3374 2007 1228 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3738 10
Size: 54 1425 343 1906 3065
Fragments arranged by size:
3065 1906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4751 5110 5269 518
Size: 648 3585 359 159 2042
Fragments arranged by size:
3585 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2305 2775 3250 3407 3939 4209 4886 6034
Size: 699 470 475 157 532 270 677 1148
Cuts at: 6034 6279 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 699 677 532 475 470 270
245 157
NarI GG'CG_CC
Cuts at: 3156 3188 3951 3156
Size: 32 763 5998
Fragments arranged by size:
5998 763 32
NcoI C'CATG_G
Cuts at: 513 1065 3444 3730 513
Size: 552 2379 286 3576
Fragments arranged by size:
3576 2379 552 286
NdeI CA'TA_TG
Cuts at: 387 2683 387
Size: 2296 4497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6793
NotI GC'GGCC_GC
Cuts at: 4240 4240
Size: 6793
NspI r_CATG'y
Cuts at: 1132 1229 2860 4151 5696 1132
Size: 97 1631 1291 1545 2229
Fragments arranged by size:
2229 1631 1545 1291 97
PasI CC'CwG_GG
Cuts at: 1348 3161 3964 1348
Size: 1813 803 4177
Fragments arranged by size:
4177 1813 803
PciI A'CATG_T
Cuts at: 2856 5692 2856
Size: 2836 3957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6793
PfoI T'CCnGG_A
Cuts at: 3744 3744
Size: 6793
PmeI GTTT'AAAC
Cuts at: 4097 4097
Size: 6793
PmlI CAC'GTG
Cuts at: 2188 2188
Size: 6793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 6375 6407 4862
Size: 32 1481 32 5248
Fragments arranged by size:
5248 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2563 2845 4009 2563
Size: 282 1164 5347
Fragments arranged by size:
5347 1164 282
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6793
PsiI TTA'TAA
Cuts at: 4363 4363
Size: 6793
PspOMI G'GGCC_C
Cuts at: 3791 3791
Size: 6793
PspXI vC'TCGA_Gb
Cuts at: 2496 2496
Size: 6793
PstI C_TGCA'G
Cuts at: 830 2325 3401 4145 830
Size: 1495 1076 744 3478
Fragments arranged by size:
3478 1495 1076 744
PvuI CG_AT'CG
Cuts at: 2001 2059 2001
Size: 58 6735
PvuII CAG'CTG
Cuts at: 2305 2305
Size: 6793
SacII CC_GC'GG
Cuts at: 3408 3408
Size: 6793
SalI G'TCGA_C
Cuts at: 1936 4135 1936
Size: 2199 4594
SanDI GG'GwC_CC
Cuts at: 2845 2845
Size: 6793
SbfI CC_TGCA'GG
Cuts at: 4145 4145
Size: 6793
ScaI AGT'ACT
Cuts at: 2826 5041 6753 2826
Size: 2215 1712 2866
Fragments arranged by size:
2866 2215 1712
SexAI A'CCwGG_T
Cuts at: 2418 2418
Size: 6793
SfcI C'TryA_G
Cuts at: 826 1045 2321 3397 4141 5276 5957 6148 826
Size: 219 1276 1076 744 1135 681 191 1471
Fragments arranged by size:
1471 1276 1135 1076 744 681 219 191
SfoI GGC'GCC
Cuts at: 3157 3189 3952 3157
Size: 32 763 5998
Fragments arranged by size:
5998 763 32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6793
SmaI CCC'GGG
Cuts at: 1872 2049 3576 4120 1872
Size: 177 1527 544 4545
Fragments arranged by size:
4545 1527 544 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2496 2804 2924 4897 5798 6060
Size: 197 926 553 308 120 1973 901 262
Cuts at: 6060 820
Size: 1553
Fragments arranged by size:
1973 1553 926 901 553 308 262 197
120
SnaBI TAC'GTA
Cuts at: 493 4090 4584 493
Size: 3597 494 2702
Fragments arranged by size:
3597 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6793
SphI G_CATG'C
Cuts at: 1132 4151 1132
Size: 3019 3774
SspI AAT'ATT
Cuts at: 5 52 2551 4717 5
Size: 47 2499 2166 2081
Fragments arranged by size:
2499 2166 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6793
StyI C'CwwG_G
Cuts at: 513 1065 2082 3444 3730 4036 4232 513
Size: 552 1017 1362 286 306 196 3074
Fragments arranged by size:
3074 1362 1017 552 306 286 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 4820 4837 4990 5175 5620 325
Size: 1255 546 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 546 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2554 2760 2824
Size: 274 80 33 51 1443 577 206 64
Cuts at: 2824 2918 3090 5039 6751 96
Size: 94 172 1949 1712 138
Fragments arranged by size:
1949 1712 1443 577 274 206 172 138
94 80 64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6753 6785 6753
Size: 32 6761
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6793
XbaI T'CTAG_A
Cuts at: 4129 4129
Size: 6793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2242 3664 2131
Size: 111 1422 5260
Fragments arranged by size:
5260 1422 111
XhoI C'TCGA_G
Cuts at: 1943 2496 2924 1943
Size: 553 428 5812
Fragments arranged by size:
5812 553 428
XmaI C'CCGG_G
Cuts at: 1870 2047 3574 4118 1870
Size: 177 1527 544 4545
Fragments arranged by size:
4545 1527 544 177
XmnI GAAnn'nnTTC
Cuts at: 2330 3214 4922 2330
Size: 884 1708 4201
Fragments arranged by size:
4201 1708 884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4601 276
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacI
SapI SfiI SrfI SwaI