Restriction map for
pFN21ASDA1852
(Circular) (Six-base) MAPSORT of: pFN21ASDA1852.seq Check: 8729 from: 1 to: 7105
pFN21ASDA1852 7105 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2322 2322
Size: 7105
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3245 4915 278
Size: 53 83 186 936 1709 1670 2468
Fragments arranged by size:
2468 1709 1670 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4448 1937
Size: 2511 4594
Acc65I G'GTAC_C
Cuts at: 4426 4426
Size: 7105
AclI AA'CG_TT
Cuts at: 1563 5232 5605 1563
Size: 3669 373 3063
Fragments arranged by size:
3669 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3400 3736 3988 5168 6552 6820 1632
Size: 1768 336 252 1180 1384 268 1917
Fragments arranged by size:
1917 1768 1384 1180 336 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6908
AflIII A'CryG_T
Cuts at: 1203 6004 6981 1203
Size: 4801 977 1327
Fragments arranged by size:
4801 1327 977
AgeI A'CCGG_T
Cuts at: 5936 5936
Size: 7105
AhdI GACnn_n'nnGTC
Cuts at: 2307 5834 2307
Size: 3527 3578
AleI CACnn'nnGTG
Cuts at: 1166 4047 1166
Size: 2881 4224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7105
AlwNI CAG_nnn'CTG
Cuts at: 4848 6420 4848
Size: 1572 5533
ApaLI G'TGCA_C
Cuts at: 5162 6318 5162
Size: 1156 5949
ApoI r'AATT_y
Cuts at: 1493 2654 2949 3424 3450 4038 4264 4414 4640
Size: 1161 295 475 26 588 226 150 226
Cuts at: 4640 4792 6901 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1161 588 475 295 226 226
152 150 26
AseI AT'TA_AT
Cuts at: 160 1032 5659 160
Size: 872 4627 1606
Fragments arranged by size:
4627 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
AvaI C'yCGr_G
Cuts at: 1870 1943 4430 1870
Size: 73 2487 4545
Fragments arranged by size:
4545 2487 73
BamHI G'GATC_C
Cuts at: 4435 4802 4435
Size: 367 6738
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 4426 5881 618
Size: 325 217 627 267 2372 1455 1842
Fragments arranged by size:
2372 1842 1455 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2113 2181 3011 3260 3596 3764 4424 1391
Size: 722 68 830 249 336 168 660 4072
Fragments arranged by size:
4072 830 722 660 336 249 168 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2608 928
Size: 971 709 5425
Fragments arranged by size:
5425 971 709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5296 5330 1903
Size: 34 3359 34 3678
Fragments arranged by size:
3678 3359 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2136 4997 6207 2136
Size: 2861 1210 3034
Fragments arranged by size:
3034 2861 1210
BclI T'GATC_A
Cuts at: 1548 2938 1548
Size: 1390 5715
BfrBI ATG'CAT
Cuts at: 3622 4294 3622
Size: 672 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2108 5716 136
Size: 107 122 71 631 605 436 3608 1525
Fragments arranged by size:
3608 1525 631 605 436 122 107 71
BglII A'GATC_T
Cuts at: 7100 7100
Size: 7105
BlpI GC'TnA_GC
Cuts at: 3343 3847 4522 3343
Size: 504 675 5926
Fragments arranged by size:
5926 675 504
Bme1580I G_kGCm'C
Cuts at: 1790 2059 5166 6322 1790
Size: 269 3107 1156 2573
Fragments arranged by size:
3107 2573 1156 269
BmgBI CAC'GTC
Cuts at: 1318 2221 1318
Size: 903 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 4836 5794
Size: 416 53 83 399 24 767 2652 958
Cuts at: 5794 442
Size: 1753
Fragments arranged by size:
2652 1753 958 767 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2733 2984 2371
Size: 362 251 6492
Fragments arranged by size:
6492 362 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2318 2521 2895 3274 3312 3610 3648
Size: 935 144 203 374 379 38 298 38
Cuts at: 3648 3732 3778 3984 4152 4236 5765 1239
Size: 84 46 206 168 84 1529 2579
Fragments arranged by size:
2579 1529 935 379 374 298 206 203
168 144 84 84 46 38 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5230 6095 6393 5230
Size: 865 298 5942
Fragments arranged by size:
5942 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 5768 882
Size: 617 795 3474 2219
Fragments arranged by size:
3474 2219 795 617
BsaAI yAC'GTr
Cuts at: 493 1204 2140 4402 4896 493
Size: 711 936 2262 494 2702
Fragments arranged by size:
2702 2262 936 711 494
BsaBI GATnn'nnATC
Cuts at: 7099 7099
Size: 7105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3242 4912 5294 5929
Size: 53 83 186 936 1709 1670 382 635
Cuts at: 5929 275
Size: 1451
Fragments arranged by size:
1709 1670 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4116 5538 5936 6210 6357 6768 4116
Size: 1422 398 274 147 411 4453
Fragments arranged by size:
4453 1422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6880
Fragments arranged by size:
6880 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2179 1186
Size: 985 8 6112
Fragments arranged by size:
6112 985 8
BseYI C'CCAG_C
Cuts at: 2182 2458 6308 6804 2182
Size: 276 3850 496 2483
Fragments arranged by size:
3850 2483 496 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6532
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4555 5316 6344 1540
Size: 275 186 2554 761 1028 2301
Fragments arranged by size:
2554 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3260 3596 3764 5166 5251 6322 3260
Size: 336 168 1402 85 1071 4043
Fragments arranged by size:
4043 1402 1071 336 168 85
BsmI GAATG_Cn'
Cuts at: 2990 3624 3833 3986 4296 4616 4709 2990
Size: 634 209 153 310 320 93 5386
Fragments arranged by size:
5386 634 320 310 209 153 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2113 2181 3011 3260 3596 3764
Size: 399 269 54 68 830 249 336 168
Cuts at: 3764 4424 5166 5251 6322 1391
Size: 660 742 85 1071 2174
Fragments arranged by size:
2174 1071 830 742 660 399 336 269
249 168 85 68 54
BspEI T'CCGG_A
Cuts at: 6768 6768
Size: 7105
BspHI T'CATG_A
Cuts at: 4994 6724 4994
Size: 1730 5375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 4460 844
Size: 1478 2138 3489
Fragments arranged by size:
3489 2138 1478
BsrBI CCG'CTC
Cuts at: 1394 1823 4992 1394
Size: 429 3169 3507
Fragments arranged by size:
3507 3169 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4107 5600 5774 65
Size: 1183 2859 1493 174 1396
Fragments arranged by size:
2859 1493 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5749 5936 1700
Size: 110 3939 187 2869
Fragments arranged by size:
3939 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3439 4195 96
Size: 3343 756 3006
Fragments arranged by size:
3343 3006 756
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7105
BssSI C'ACGA_G
Cuts at: 5165 6177 5165
Size: 1012 6093
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7105
BstBI TT'CG_AA
Cuts at: 2007 5909 2007
Size: 3902 3203
BstEII G'GTnAC_C
Cuts at: 1184 2309 2797 1184
Size: 1125 488 5492
Fragments arranged by size:
5492 1125 488
BstYI r'GATC_y
Cuts at: 1972 4082 4435 4802 5187 5204 6656 6742 6754
Size: 2110 353 367 385 17 1452 86 12
Cuts at: 6754 7100 1972
Size: 346 1977
Fragments arranged by size:
2110 1977 1452 385 367 353 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2346 2346
Size: 7105
BtgI C'CryG_G
Cuts at: 513 1065 2022 2039 2096 2226 2381 2491 513
Size: 552 957 17 57 130 155 110 5127
Fragments arranged by size:
5127 957 552 155 130 110 57 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6366
Fragments arranged by size:
6366 683 56
BtsI GCAGTG_nn'
Cuts at: 3204 4327 4621 5415 5435 3204
Size: 1123 294 794 20 4874
Fragments arranged by size:
4874 1123 794 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7105
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2228 2263 3217 3252 3973 4008 529
Size: 35 1664 35 954 35 721 35 3626
Fragments arranged by size:
3626 1664 954 721 35 35 35 35
DraI TTT'AAA
Cuts at: 3643 4409 3643
Size: 766 6339
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6112 809
Size: 507 4796 1802
Fragments arranged by size:
4796 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 4552 5441
Size: 54 1425 50 275 18 393 2329 889
Cuts at: 5441 7070 8
Size: 1629 43
Fragments arranged by size:
2329 1629 1425 889 393 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4552 1537
Size: 275 2740 4090
Fragments arranged by size:
4090 2740 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 2370 4380 5035 1353
Size: 885 132 2010 655 3423
Fragments arranged by size:
3423 2010 885 655 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5692 6066 6212 387
Size: 1064 138 4103 374 146 1280
Fragments arranged by size:
4103 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3258 3594 3762 3258
Size: 336 168 6601
Fragments arranged by size:
6601 336 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2318 2521 2895 3274 3312 3400
Size: 393 542 144 203 374 379 38 88
Cuts at: 3400 3610 3648 3732 3736 3778 3984 3988 4152
Size: 210 38 84 4 42 206 4 164
Cuts at: 4152 4236 5168 5765 6552 6820 1239
Size: 84 932 597 787 268 1524
Fragments arranged by size:
1524 932 787 597 542 393 379 374
268 210 206 203 164 144 88 84
84 42 38 38 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2156 2156
Size: 7105
EcoO109I rG'GnC_Cy
Cuts at: 2274 2274
Size: 7105
EcoRI G'AATT_C
Cuts at: 2654 2949 3424 3450 4038 4264 4414 4792 2654
Size: 295 475 26 588 226 150 378 4967
Fragments arranged by size:
4967 588 475 378 295 226 150 26
FspI TGC'GCA
Cuts at: 5611 5611
Size: 7105
HaeII r_GCGC'y
Cuts at: 1678 6252 1678
Size: 4574 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2369 2401 3583
Size: 32 135 32 721 32 44 32 1182
Cuts at: 3583 3615 3751 3783 4169 4201 5746 5778 5820
Size: 32 136 32 386 32 1545 32 42
Cuts at: 5820 5852 1373
Size: 32 2626
Fragments arranged by size:
2626 1545 1182 721 386 136 135 44
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 4449 4695 669
Size: 988 281 154 2357 246 3079
Fragments arranged by size:
3079 2357 988 281 246 154
HindIII A'AGCT_T
Cuts at: 748 3663 748
Size: 2915 4190
HpaI GTT'AAC
Cuts at: 4695 4695
Size: 7105
KpnI G_GTAC'C
Cuts at: 4430 4430
Size: 7105
MfeI C'AATT_G
Cuts at: 4704 4704
Size: 7105
MluI A'CGCG_T
Cuts at: 6981 6981
Size: 7105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 2484 2741 3022 6219 6403 1305
Size: 1038 141 257 281 3197 184 2007
Fragments arranged by size:
3197 2007 1038 281 257 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5283
Fragments arranged by size:
5283 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2934 3727 4047 4147 5063 5422 5581
Size: 648 1768 793 320 100 916 359 159
Cuts at: 5581 518
Size: 2042
Fragments arranged by size:
2042 1768 916 793 648 359 320 159
100
MspA1I CmG'CkG
Cuts at: 1606 2087 2257 3420 3588 3756 4521 5198 6346
Size: 481 170 1163 168 168 765 677 1148
Cuts at: 6346 6591 1606
Size: 245 2120
Fragments arranged by size:
2120 1163 1148 765 677 481 245 170
168 168
NcoI C'CATG_G
Cuts at: 513 1065 2022 2096 2491 513
Size: 552 957 74 395 5127
Fragments arranged by size:
5127 957 552 395 74
NdeI CA'TA_TG
Cuts at: 387 3612 387
Size: 3225 3880
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7105
NotI GC'GGCC_GC
Cuts at: 4552 4552
Size: 7105
NsiI A_TGCA'T
Cuts at: 3624 4296 3624
Size: 672 6433
NspI r_CATG'y
Cuts at: 1132 1229 2403 2753 4463 6008 1132
Size: 97 1174 350 1710 1545 2229
Fragments arranged by size:
2229 1710 1545 1174 350 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7105
PciI A'CATG_T
Cuts at: 6004 6004
Size: 7105
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7105
PmeI GTTT'AAAC
Cuts at: 4409 4409
Size: 7105
PmlI CAC'GTG
Cuts at: 2140 2140
Size: 7105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5174 5206 6687 6719 5174
Size: 32 1481 32 5560
Fragments arranged by size:
5560 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2274 2274
Size: 7105
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7105
PsiI TTA'TAA
Cuts at: 4675 4675
Size: 7105
PstI C_TGCA'G
Cuts at: 830 3211 4457 830
Size: 2381 1246 3478
Fragments arranged by size:
3478 2381 1246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7105
PvuII CAG'CTG
Cuts at: 2087 2257 3420 3588 3756 2087
Size: 170 1163 168 168 5436
Fragments arranged by size:
5436 1163 170 168 168
SacI G_AGCT'C
Cuts at: 3260 3596 3764 3260
Size: 336 168 6601
Fragments arranged by size:
6601 336 168
SalI G'TCGA_C
Cuts at: 1936 4447 1936
Size: 2511 4594
SapI GCTCTTCn'nnn_
Cuts at: 2370 2370
Size: 7105
SbfI CC_TGCA'GG
Cuts at: 4457 4457
Size: 7105
ScaI AGT'ACT
Cuts at: 2634 5353 7065 2634
Size: 2719 1712 2674
Fragments arranged by size:
2719 2674 1712
SfcI C'TryA_G
Cuts at: 826 1045 3123 3207 3919 4093 4453 5588 6269
Size: 219 2078 84 712 174 360 1135 681
Cuts at: 6269 6460 826
Size: 191 1471
Fragments arranged by size:
2078 1471 1135 712 681 360 219 191
174 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7105
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7105
SmaI CCC'GGG
Cuts at: 1872 4432 1872
Size: 2560 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5209 6110 6372 820
Size: 197 926 3266 901 262 1553
Fragments arranged by size:
3266 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4402 4896 493
Size: 3909 494 2702
Fragments arranged by size:
3909 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7105
SphI G_CATG'C
Cuts at: 1132 4463 1132
Size: 3331 3774
SspI AAT'ATT
Cuts at: 5 52 5029 5
Size: 47 4977 2081
Fragments arranged by size:
4977 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7105
StyI C'CwwG_G
Cuts at: 513 1065 2022 2096 2491 2528 3029 4544 513
Size: 552 957 74 395 37 501 1515 3074
Fragments arranged by size:
3074 1515 957 552 501 395 74 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2416 3042 5132 5149 5302 5487
Size: 1255 672 164 626 2090 17 153 185
Cuts at: 5487 5932 325
Size: 445 1498
Fragments arranged by size:
2090 1498 1255 672 626 445 185 164
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2594 2632 2894
Size: 274 80 33 51 1443 617 38 262
Cuts at: 2894 3439 3563 3731 4195 5351 7063 96
Size: 545 124 168 464 1156 1712 138
Fragments arranged by size:
1712 1443 1156 617 545 464 274 262
168 138 124 80 51 38 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7065 7097 7065
Size: 32 7073
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7105
XbaI T'CTAG_A
Cuts at: 4441 4441
Size: 7105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2103 2103
Size: 7105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7105
XmaI C'CCGG_G
Cuts at: 1870 4430 1870
Size: 2560 4545
XmnI GAAnn'nnTTC
Cuts at: 3497 3581 3706 5234 3497
Size: 84 125 1528 5368
Fragments arranged by size:
5368 1528 125 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3243 4913 276
Size: 53 83 186 936 1709 1670 2468
Fragments arranged by size:
2468 1709 1670 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI