Restriction map for
pFN21ASDA1848
(Circular) (Six-base) MAPSORT of: pFN21ASDA1848.seq Check: 6142 from: 1 to: 5941
pFN21ASDA1848 5941 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 2790
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3141 3284 1937
Size: 1204 143 4594
Fragments arranged by size:
4594 1204 143
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 4004 5388 5656 1632
Size: 938 1434 1384 268 1917
Fragments arranged by size:
1917 1434 1384 938 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 2582 4840 5817 1203
Size: 1379 2258 977 1327
Fragments arranged by size:
2258 1379 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2771 2903 1166
Size: 1605 132 4204
Fragments arranged by size:
4204 1605 132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5941
AlwNI CAG_nnn'CTG
Cuts at: 2384 3684 5256 2384
Size: 1300 1572 3069
Fragments arranged by size:
3069 1572 1300
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2099 3250 3476 3628 5737 1493
Size: 606 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 606 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2154 2232 3069 3266 1870
Size: 73 130 81 78 837 197 4545
Fragments arranged by size:
4545 837 197 130 81 78 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2599 2882 2897 2909 2980
Size: 325 217 627 812 283 15 12 71
Cuts at: 2980 3262 4717 618
Size: 282 1455 1842
Fragments arranged by size:
1842 1455 812 627 325 283 282 217
71 15 12
BanII G_rGCy'C
Cuts at: 1391 2697 2922 3132 3260 1391
Size: 1306 225 210 128 4072
Fragments arranged by size:
4072 1306 225 210 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2123 2366 2486 928
Size: 971 224 243 120 4383
Fragments arranged by size:
4383 971 243 224 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 2367 1548
Size: 819 5122
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2999 3119 3153
Size: 107 122 71 631 605 1327 120 34
Cuts at: 3153 4552 136
Size: 1399 1525
Fragments arranged by size:
1525 1399 1327 631 605 122 120 107
71 34
BglII A'GATC_T
Cuts at: 2187 5936 2187
Size: 3749 2192
BlpI GC'TnA_GC
Cuts at: 3230 3358 3230
Size: 128 5813
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2912 2985 4002 5158 1790
Size: 443 679 73 1017 1156 2573
Fragments arranged by size:
2573 1156 1017 679 443 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 2405 3672
Size: 416 53 83 399 24 803 185 1267
Cuts at: 3672 4630 442
Size: 958 1753
Fragments arranged by size:
1753 1267 958 803 416 399 185 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 2771 4601 1239
Size: 1247 285 1830 2579
Fragments arranged by size:
2579 1830 1247 285
Bpu10I CC'TnA_GC
Cuts at: 2936 2936
Size: 5941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2212 2263 4066 4931 5229 2212
Size: 51 1803 865 298 2924
Fragments arranged by size:
2924 1803 865 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2583 2773 3238 3732 493
Size: 711 1379 190 465 494 2702
Fragments arranged by size:
2702 1379 711 494 465 190
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 2518 3748 4130
Size: 53 83 186 936 515 470 1230 382
Cuts at: 4130 4765 275
Size: 635 1451
Fragments arranged by size:
1451 1230 936 635 515 470 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 2414 2444 2583
Size: 30 165 30 831 30 150 30 139
Cuts at: 2583 2613 1178
Size: 30 4506
Fragments arranged by size:
4506 831 165 150 139 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2255 2495 1186
Size: 922 147 240 4632
Fragments arranged by size:
4632 922 240 147
BseYI C'CCAG_C
Cuts at: 2335 2866 5144 5640 2335
Size: 531 2278 496 2636
Fragments arranged by size:
2636 2278 531 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 1163
Size: 573 366 5002
Fragments arranged by size:
5002 573 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 4152 5180 1540
Size: 275 186 1390 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2697 3078 3132 4002 4087 5158 2697
Size: 381 54 870 85 1071 3480
Fragments arranged by size:
3480 1071 870 381 85 54
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2747 2747
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2697 2912 2922 2985 3078 3132
Size: 399 443 464 215 10 63 93 54
Cuts at: 3132 3260 4002 4087 5158 1391
Size: 128 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 464 443 399 215 128
93 85 63 54 10
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2790 3296 844
Size: 1946 506 3489
Fragments arranged by size:
3489 1946 506
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2514 2721 4585 4772 1700
Size: 110 704 207 1864 187 2869
Fragments arranged by size:
2869 1864 704 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2795 4001 5013 2795
Size: 1206 1012 3723
Fragments arranged by size:
3723 1206 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 2007 4745 2007
Size: 2738 3203
BstEII G'GTnAC_C
Cuts at: 1184 2284 1184
Size: 1100 4841
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2335
Size: 5941
BstYI r'GATC_y
Cuts at: 1972 2187 3271 3638 4023 4040 5492 5578 5590
Size: 215 1084 367 385 17 1452 86 12
Cuts at: 5590 5936 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1084 385 367 346 215 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2403 513
Size: 552 957 381 4051
Fragments arranged by size:
4051 957 552 381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5202
Fragments arranged by size:
5202 683 56
BtsI GCAGTG_nn'
Cuts at: 2955 3109 3457 4251 4271 2955
Size: 154 348 794 20 4625
Fragments arranged by size:
4625 794 348 154 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2270 4948 809
Size: 507 954 2678 1802
Fragments arranged by size:
2678 1802 954 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2325 2643
Size: 54 1425 50 275 18 274 221 318
Cuts at: 2643 2964 2991 3388 4277 5906 8
Size: 321 27 397 889 1629 43
Fragments arranged by size:
1629 1425 889 397 321 318 275 274
221 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3388 1537
Size: 275 1576 4090
Fragments arranged by size:
4090 1576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3027 3871 1353
Size: 1674 844 3423
Fragments arranged by size:
3423 1674 844
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 3130 2695
Size: 435 5506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2486 2570 2771 4004 4601 5388 5656
Size: 393 854 84 201 1233 597 787 268
Cuts at: 5656 1239
Size: 1524
Fragments arranged by size:
1524 1233 854 787 597 393 268 201
84
EcoNI CCTnn'n_nnAGG
Cuts at: 3032 3032
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2272 3057 3205 2272
Size: 785 148 5008
Fragments arranged by size:
5008 785 148
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FspI TGC'GCA
Cuts at: 2456 4447 2456
Size: 1991 3950
HaeII r_GCGC'y
Cuts at: 1678 2652 5088 1678
Size: 974 2436 2531
Fragments arranged by size:
2531 2436 974
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2608 2640 4582 4614 4656
Size: 32 135 32 1036 32 1942 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1942 1036 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 5055 5239 1305
Size: 726 3024 184 2007
Fragments arranged by size:
3024 2007 726 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 2993 10
Size: 54 1425 343 274 887 2958
Fragments arranged by size:
2958 1425 887 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2771 2903 3899 4258 4417 518
Size: 648 1605 132 996 359 159 2042
Fragments arranged by size:
2042 1605 996 648 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 2405 2866 3357 4034 5182 5427 1606
Size: 799 461 491 677 1148 245 2120
Fragments arranged by size:
2120 1148 799 677 491 461 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4432
Fragments arranged by size:
4432 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 2848 3299 4844 1132
Size: 97 1619 451 1545 2229
Fragments arranged by size:
2229 1619 1545 451 97
PasI CC'CwG_GG
Cuts at: 1348 2878 1348
Size: 1530 4411
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2175 2848 1108
Size: 1067 673 4201
Fragments arranged by size:
4201 1067 673
PfoI T'CCnGG_A
Cuts at: 2463 2463
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PmlI CAC'GTG
Cuts at: 2583 2773 2583
Size: 190 5751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 3057 3205 2272
Size: 785 148 5008
Fragments arranged by size:
5008 785 148
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2217 2569 3293 830
Size: 1387 352 724 3478
Fragments arranged by size:
3478 1387 724 352
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2866 2866
Size: 5941
SacI G_AGCT'C
Cuts at: 2697 3132 2697
Size: 435 5506
SacII CC_GC'GG
Cuts at: 2406 2406
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 3057 3057
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2213 2565 3142 3289 4424 5105 5296
Size: 219 1168 352 577 147 1135 681 191
Cuts at: 5296 826
Size: 1471
Fragments arranged by size:
1471 1168 1135 681 577 352 219 191
147
SfiI GGCCn_nnn'nGGCC
Cuts at: 2999 2999
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2075 2156 3268 1872
Size: 203 81 1112 4545
Fragments arranged by size:
4545 1112 203 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2191 2278 4045 4946 5208 820
Size: 197 926 248 87 1767 901 262 1553
Fragments arranged by size:
1767 1553 926 901 262 248 197 87
SnaBI TAC'GTA
Cuts at: 493 3238 3732 493
Size: 2745 494 2702
Fragments arranged by size:
2745 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SrfI GCCC'GGGC
Cuts at: 2156 2156
Size: 5941
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 3017 1517
Size: 1500 4441
StyI C'CwwG_G
Cuts at: 513 1065 2022 2559 3380 513
Size: 552 957 537 821 3074
Fragments arranged by size:
3074 957 821 552 537
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2965 3968 3985 4138 4323 4768 325
Size: 1255 1385 1003 17 153 185 445 1498
Fragments arranged by size:
1498 1385 1255 1003 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 4187 5899
Size: 274 80 33 51 1443 333 1877 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1877 1712 1443 333 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 2583 2615 5901 5933 2414
Size: 32 137 32 3286 32 2422
Fragments arranged by size:
3286 2422 137 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2233 2974 2233
Size: 741 5200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 2073 2154 3266 1870
Size: 203 81 1112 4545
Fragments arranged by size:
4545 1112 203 81
XmnI GAAnn'nnTTC
Cuts at: 2145 4070 2145
Size: 1925 4016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SapI SexAI SfoI SwaI