Restriction map for
pFN21ASDA1831
(Circular) (Six-base) MAPSORT of: pFN21ASDA1831.seq Check: 2919 from: 1 to: 6613
pFN21ASDA1831 6613 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2722 4423 278
Size: 53 83 186 936 1186 1701 2468
Fragments arranged by size:
2468 1701 1186 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3956 1937
Size: 2019 4594
Acc65I G'GTAC_C
Cuts at: 3934 3934
Size: 6613
AclI AA'CG_TT
Cuts at: 1563 4740 5113 1563
Size: 3177 373 3063
Fragments arranged by size:
3177 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 4676 6060 6328 1632
Size: 683 2361 1384 268 1917
Fragments arranged by size:
2361 1917 1384 683 268
AfeI AGC'GCT
Cuts at: 2918 2918
Size: 6613
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6416
AflIII A'CryG_T
Cuts at: 1203 5512 6489 1203
Size: 4309 977 1327
Fragments arranged by size:
4309 1327 977
AgeI A'CCGG_T
Cuts at: 5444 5444
Size: 6613
AhdI GACnn_n'nnGTC
Cuts at: 2638 5342 2638
Size: 2704 3909
AleI CACnn'nnGTG
Cuts at: 1166 2861 1166
Size: 1695 4918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3077 1184
Size: 1893 4720
AlwNI CAG_nnn'CTG
Cuts at: 2145 3268 4356 5928 2145
Size: 1123 1088 1572 2830
Fragments arranged by size:
2830 1572 1123 1088
ApaI G_GGCC'C
Cuts at: 2120 2190 2622 2817 3597 3697 2120
Size: 70 432 195 780 100 5036
Fragments arranged by size:
5036 780 432 195 100 70
ApaLI G'TGCA_C
Cuts at: 2110 3035 4670 5826 2110
Size: 925 1635 1156 2897
Fragments arranged by size:
2897 1635 1156 925
ApoI r'AATT_y
Cuts at: 1493 3922 4148 4300 6409 1493
Size: 2429 226 152 2109 1697
Fragments arranged by size:
2429 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2587 2595 2621 3751 3788 3938
Size: 73 87 557 8 26 1130 37 150
Cuts at: 3938 1870
Size: 4545
Fragments arranged by size:
4545 1130 557 150 87 73 37 26
8
BamHI G'GATC_C
Cuts at: 3389 3943 4310 3389
Size: 554 367 5692
Fragments arranged by size:
5692 554 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2402 3119 3771 3934
Size: 325 217 627 336 279 717 652 163
Cuts at: 3934 5389 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 717 652 627 336 325 279
217 163
BanII G_rGCy'C
Cuts at: 1391 2120 2190 2349 2594 2622 2706 2817 2852
Size: 729 70 159 245 28 84 111 35
Cuts at: 2852 3336 3458 3597 3678 3697 3720 3746 3789
Size: 484 122 139 81 19 23 26 43
Cuts at: 3789 3798 3932 1391
Size: 9 134 4072
Fragments arranged by size:
4072 729 484 245 159 139 134 122
111 84 81 70 43 35 28 26
23 19 9
BbeI G_GCGC'C
Cuts at: 2127 3123 2127
Size: 996 5617
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2170 3638 928
Size: 971 271 1468 3903
Fragments arranged by size:
3903 1468 971 271
BbvCI CC'TCA_GC
Cuts at: 3564 3689 3872 3564
Size: 125 183 6305
Fragments arranged by size:
6305 183 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2884 2918 3797 3831 4804 4838 1903
Size: 34 947 34 879 34 973 34 3678
Fragments arranged by size:
3678 973 947 879 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4505 5715 4505
Size: 1210 5403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2994 3233 5224
Size: 107 122 71 631 605 1322 239 1991
Cuts at: 5224 136
Size: 1525
Fragments arranged by size:
1991 1525 1322 631 605 239 122 107
71
BglII A'GATC_T
Cuts at: 6608 6608
Size: 6613
BlpI GC'TnA_GC
Cuts at: 2696 4030 2696
Size: 1334 5279
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2114 2120 2155 2190 2466 2622 2817
Size: 305 19 6 35 35 276 156 195
Cuts at: 2817 3039 3597 3697 4674 5830 1790
Size: 222 558 100 977 1156 2573
Fragments arranged by size:
2573 1156 977 558 305 276 222 195
156 100 35 35 19 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6613
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2180 4344 5302
Size: 416 53 83 399 24 763 2164 958
Cuts at: 5302 442
Size: 1753
Fragments arranged by size:
2164 1753 958 763 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 6613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3475 3489 5273 1239
Size: 2236 14 1784 2579
Fragments arranged by size:
2579 2236 1784 14
Bpu10I CC'TnA_GC
Cuts at: 3564 3689 3714 3872 3564
Size: 125 25 158 6305
Fragments arranged by size:
6305 158 125 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4738 5603 5901 4738
Size: 865 298 5450
Fragments arranged by size:
5450 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 5276 882
Size: 617 879 2898 2219
Fragments arranged by size:
2898 2219 879 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 3910 4404 493
Size: 711 864 1842 494 2702
Fragments arranged by size:
2702 1842 864 711 494
BsaBI GATnn'nnATC
Cuts at: 6607 6607
Size: 6613
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2175 2537 2628
Size: 53 83 186 936 591 51 362 91
Cuts at: 2628 2719 2907 3120 3183 4420 4802 5437 275
Size: 91 188 213 63 1237 382 635 1451
Fragments arranged by size:
1451 1237 936 635 591 382 362 213
188 186 91 91 83 63 53 51
BsaWI w'CCGG_w
Cuts at: 2786 5046 5444 5718 5865 6276 2786
Size: 2260 398 274 147 411 3123
Fragments arranged by size:
3123 2260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2999 3029 1178
Size: 30 165 30 1596 30 4762
Fragments arranged by size:
4762 1596 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3020 3409 3527 3605 3668 3671 3890 1186
Size: 1834 389 118 78 63 3 219 3909
Fragments arranged by size:
3909 1834 389 219 118 78 63 3
BseYI C'CCAG_C
Cuts at: 2076 2148 2239 2284 2950 3404 3575 3697 3767
Size: 72 91 45 666 454 171 122 70
Cuts at: 3767 3819 3894 5816 6312 2076
Size: 52 75 1922 496 2377
Fragments arranged by size:
2377 1922 666 496 454 171 122 91
75 72 70 52 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6040
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2667 2823 4063 4824 5852 1540
Size: 275 186 666 156 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 666 275 186 156
BsiHKAI G_wGCw'C
Cuts at: 2114 3039 3789 4674 4759 5830 2114
Size: 925 750 885 85 1071 2897
Fragments arranged by size:
2897 1071 925 885 750 85
BsmI GAATG_Cn'
Cuts at: 3881 4124 4217 3881
Size: 243 93 6277
Fragments arranged by size:
6277 243 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3594 3594
Size: 6613
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2114 2120 2155 2190 2349 2466
Size: 399 305 19 6 35 35 159 117
Cuts at: 2466 2594 2622 2706 2817 2852 3039 3336 3458
Size: 128 28 84 111 35 187 297 122
Cuts at: 3458 3597 3678 3697 3720 3746 3789 3798 3932
Size: 139 81 19 23 26 43 9 134
Cuts at: 3932 4674 4759 5830 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 297 187 159
139 134 128 122 117 111 85 84
81 43 35 35 35 28 26 23
19 19 9 6
BspEI T'CCGG_A
Cuts at: 6276 6276
Size: 6613
BspHI T'CATG_A
Cuts at: 4502 6232 4502
Size: 1730 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3276 3968 844
Size: 2432 692 3489
Fragments arranged by size:
3489 2432 692
BsrBI CCG'CTC
Cuts at: 1394 1823 2711 4500 1394
Size: 429 888 1789 3507
Fragments arranged by size:
3507 1789 888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5108 5282 65
Size: 1183 3860 174 1396
Fragments arranged by size:
3860 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2666 2958 3027 5257 5444 1700
Size: 110 856 292 69 2230 187 2869
Fragments arranged by size:
2869 2230 856 292 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6613
BssHII G'CGCG_C
Cuts at: 1925 2881 1925
Size: 956 5657
BssSI C'ACGA_G
Cuts at: 4673 5685 4673
Size: 1012 5601
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3277 794
Size: 2483 4130
BstBI TT'CG_AA
Cuts at: 2007 5417 2007
Size: 3410 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6613
BstXI CCAn_nnnn'nTGG
Cuts at: 3841 3841
Size: 6613
BstYI r'GATC_y
Cuts at: 1972 3389 3943 4310 4695 4712 6164 6250 6262
Size: 1417 554 367 385 17 1452 86 12
Cuts at: 6262 6608 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1417 554 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3510 3737 3510
Size: 227 6386
BtgI C'CryG_G
Cuts at: 513 1065 2272 2437 3228 3855 513
Size: 552 1207 165 791 627 3271
Fragments arranged by size:
3271 1207 791 627 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3706 487
Size: 683 56 2480 3394
Fragments arranged by size:
3394 2480 683 56
BtsI GCAGTG_nn'
Cuts at: 3204 3217 3458 4129 4923 4943 3204
Size: 13 241 671 794 20 4874
Fragments arranged by size:
4874 794 671 241 20 13
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6578
DraI TTT'AAA
Cuts at: 3917 3917
Size: 6613
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3110 5620 809
Size: 507 1794 2510 1802
Fragments arranged by size:
2510 1802 1794 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 2613 2664
Size: 54 1425 50 275 18 688 95 51
Cuts at: 2664 2820 2853 3246 4060 4949 6578 8
Size: 156 33 393 814 889 1629 43
Fragments arranged by size:
1629 1425 889 814 688 393 275 156
95 54 51 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2664 2820 4060 1537
Size: 275 852 156 1240 4090
Fragments arranged by size:
4090 1240 852 275 156
EarI CTCTTCn'nnn_
Cuts at: 1353 3666 3777 4543 1353
Size: 2313 111 766 3423
Fragments arranged by size:
3423 2313 766 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2208 2685 2730 5200 5574 5720
Size: 1064 138 619 477 45 2470 374 146
Cuts at: 5720 387
Size: 1280
Fragments arranged by size:
2470 1280 1064 619 477 374 146 138
45
EcoICRI GAG'CTC
Cuts at: 3787 3787
Size: 6613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 3475 3489 4676 5273 6060 6328
Size: 393 683 1160 14 1187 597 787 268
Cuts at: 6328 1239
Size: 1524
Fragments arranged by size:
1524 1187 1160 787 683 597 393 268
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2843 3191 3341 3468 2843
Size: 348 150 127 5988
Fragments arranged by size:
5988 348 150 127
EcoO109I rG'GnC_Cy
Cuts at: 2117 2186 2814 3265 3286 3482 3593 3693 3694
Size: 69 628 451 21 196 111 100 1
Cuts at: 3694 3758 2117
Size: 64 4972
Fragments arranged by size:
4972 628 451 196 111 100 69 64
21 1
EcoRI G'AATT_C
Cuts at: 3922 4300 3922
Size: 378 6235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 6613
FspI TGC'GCA
Cuts at: 2756 5119 2756
Size: 2363 4250
HaeII r_GCGC'y
Cuts at: 1678 2127 2437 2483 2831 2920 3123 3154 5760
Size: 449 310 46 348 89 203 31 2606
Cuts at: 5760 1678
Size: 2531
Fragments arranged by size:
2606 2531 449 348 310 203 89 46
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 5254 5286 5328
Size: 32 135 32 1052 32 2598 32 42
Cuts at: 5328 5360 1373
Size: 32 2626
Fragments arranged by size:
2626 2598 1052 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3957 4203 669
Size: 988 281 2019 246 3079
Fragments arranged by size:
3079 2019 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3501 748
Size: 2753 3860
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KasI G'GCGC_C
Cuts at: 2123 3119 2123
Size: 996 5617
KpnI G_GTAC'C
Cuts at: 3938 3938
Size: 6613
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 2946 3348 5727 5911 1305
Size: 1113 528 402 2379 184 2007
Fragments arranged by size:
2379 2007 1113 528 402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3248 10
Size: 54 1425 343 1416 3375
Fragments arranged by size:
3375 1425 1416 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 3725 4571 4930 5089 518
Size: 648 1695 864 846 359 159 2042
Fragments arranged by size:
2042 1695 864 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2262 2274 2307 2700 2811 2898 3167
Size: 536 120 12 33 393 111 87 269
Cuts at: 3167 3688 3800 4029 4706 5854 6099 1606
Size: 521 112 229 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 521 393 269 245
229 120 112 111 87 33 12
NaeI GCC'GGC
Cuts at: 2668 2668
Size: 6613
NarI GG'CG_CC
Cuts at: 2124 3120 2124
Size: 996 5617
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6061
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NgoMIV G'CCGG_C
Cuts at: 2666 2666
Size: 6613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 3971 5516 1132
Size: 97 2742 1545 2229
Fragments arranged by size:
2742 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2128 2731 3353 1348
Size: 780 603 622 4608
Fragments arranged by size:
4608 780 622 603
PciI A'CATG_T
Cuts at: 5512 5512
Size: 6613
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3504 1108
Size: 2396 4217
PfoI T'CCnGG_A
Cuts at: 2364 3466 3864 2364
Size: 1102 398 5113
Fragments arranged by size:
5113 1102 398
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6195 6227 4682
Size: 32 1481 32 5068
Fragments arranged by size:
5068 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3265 3482 3758 3265
Size: 217 276 6120
Fragments arranged by size:
6120 276 217
PshAI GACnn'nnGTC
Cuts at: 1613 3480 1613
Size: 1867 4746
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PspOMI G'GGCC_C
Cuts at: 2116 2186 2618 2813 3593 3693 2116
Size: 70 432 195 780 100 5036
Fragments arranged by size:
5036 780 432 195 100 70
PspXI vC'TCGA_Gb
Cuts at: 3751 3751
Size: 6613
PstI C_TGCA'G
Cuts at: 830 3273 3629 3965 830
Size: 2443 356 336 3478
Fragments arranged by size:
3478 2443 356 336
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
RsrII CG'GwC_CG
Cuts at: 2789 2789
Size: 6613
SacI G_AGCT'C
Cuts at: 3789 3789
Size: 6613
SacII CC_GC'GG
Cuts at: 2275 2275
Size: 6613
SalI G'TCGA_C
Cuts at: 1936 3955 1936
Size: 2019 4594
SanDI GG'GwC_CC
Cuts at: 3482 3482
Size: 6613
SapI GCTCTTCn'nnn_
Cuts at: 3666 3777 3666
Size: 111 6502
SbfI CC_TGCA'GG
Cuts at: 3965 3965
Size: 6613
ScaI AGT'ACT
Cuts at: 4861 6573 4861
Size: 1712 4901
SfcI C'TryA_G
Cuts at: 826 1045 3269 3625 3961 5096 5777 5968 826
Size: 219 2224 356 336 1135 681 191 1471
Fragments arranged by size:
2224 1471 1135 681 356 336 219 191
SfoI GGC'GCC
Cuts at: 2125 3121 2125
Size: 996 5617
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6613
SmaI CCC'GGG
Cuts at: 1872 2032 2597 2623 3940 1872
Size: 160 565 26 1317 4545
Fragments arranged by size:
4545 1317 565 160 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4717 5618 5880 820
Size: 197 926 1808 966 901 262 1553
Fragments arranged by size:
1808 1553 966 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3910 4404 493
Size: 3417 494 2702
Fragments arranged by size:
3417 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 3971 1132
Size: 2839 3774
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 3814 1517
Size: 2297 4316
StyI C'CwwG_G
Cuts at: 513 1065 4052 513
Size: 552 2987 3074
Fragments arranged by size:
3074 2987 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3759 4640 4657 4810 4995 5440 325
Size: 1255 2179 881 17 153 185 445 1498
Fragments arranged by size:
2179 1498 1255 881 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 3015 4859
Size: 274 80 33 51 1443 159 879 1844
Cuts at: 4859 6571 96
Size: 1712 138
Fragments arranged by size:
1844 1712 1443 879 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6573 6605 6573
Size: 32 6581
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6613
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3727 3841 3727
Size: 114 6499
XhoI C'TCGA_G
Cuts at: 1943 3751 1943
Size: 1808 4805
XmaI C'CCGG_G
Cuts at: 1870 2030 2595 2621 3938 1870
Size: 160 565 26 1317 4545
Fragments arranged by size:
4545 1317 565 160 26
XmnI GAAnn'nnTTC
Cuts at: 4742 4742
Size: 6613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2720 4421 276
Size: 53 83 186 936 1186 1701 2468
Fragments arranged by size:
2468 1701 1186 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI
PsrI PvuII SexAI SfiI SrfI SwaI