Restriction map for
pFN21ASDA1819
(Circular) (Six-base) MAPSORT of: pFN21ASDA1819.seq Check: 6550 from: 1 to: 8188
pFN21ASDA1819 8188 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2442 4993 2442
Size: 2551 5637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4955 5998 278
Size: 53 83 186 936 3419 1043 2468
Fragments arranged by size:
3419 2468 1043 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4562 5531 1937
Size: 2625 969 4594
Fragments arranged by size:
4594 2625 969
Acc65I G'GTAC_C
Cuts at: 5509 5509
Size: 8188
AclI AA'CG_TT
Cuts at: 1563 6315 6688 1563
Size: 4752 373 3063
Fragments arranged by size:
4752 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3222 4055 4073 4650 6251 7635 7903 1632
Size: 1590 833 18 577 1601 1384 268 1917
Fragments arranged by size:
1917 1601 1590 1384 833 577 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7991
AflIII A'CryG_T
Cuts at: 1203 3561 7087 8064 1203
Size: 2358 3526 977 1327
Fragments arranged by size:
3526 2358 1327 977
AgeI A'CCGG_T
Cuts at: 7019 7019
Size: 8188
AhdI GACnn_n'nnGTC
Cuts at: 6917 6917
Size: 8188
AleI CACnn'nnGTG
Cuts at: 1166 5322 1166
Size: 4156 4032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4470 1184
Size: 3286 4902
AlwNI CAG_nnn'CTG
Cuts at: 2251 2434 2947 4345 5011 5423 5931 7503 2251
Size: 183 513 1398 666 412 508 1572 2936
Fragments arranged by size:
2936 1572 1398 666 513 508 412 183
ApaI G_GGCC'C
Cuts at: 3149 5372 3149
Size: 2223 5965
ApaLI G'TGCA_C
Cuts at: 2117 6245 7401 2117
Size: 4128 1156 2904
Fragments arranged by size:
4128 2904 1156
ApoI r'AATT_y
Cuts at: 1493 2637 4828 5497 5723 5875 7984 1493
Size: 1144 2191 669 226 152 2109 1697
Fragments arranged by size:
2191 2109 1697 1144 669 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6742 160
Size: 872 5710 1606
Fragments arranged by size:
5710 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8188
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 5513 1870
Size: 73 167 3403 4545
Fragments arranged by size:
4545 3403 167 73
AvrII C'CTAG_G
Cuts at: 3080 3080
Size: 8188
BamHI G'GATC_C
Cuts at: 2929 5518 5885 2929
Size: 2589 367 5232
Fragments arranged by size:
5232 2589 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3594 5509 6964 618
Size: 325 217 627 1807 1915 1455 1842
Fragments arranged by size:
1915 1842 1807 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2102 2149 2219 3149 3506 3675 3917
Size: 697 14 47 70 930 357 169 242
Cuts at: 3917 5372 5507 1391
Size: 1455 135 4072
Fragments arranged by size:
4072 1455 930 697 357 242 169 135
70 47 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5420 928
Size: 971 3521 3696
Fragments arranged by size:
3696 3521 971
BbvCI CC'TCA_GC
Cuts at: 3162 3167 3580 5345 3162
Size: 5 413 1765 6005
Fragments arranged by size:
6005 1765 413 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6379 6413 1903
Size: 34 4442 34 3678
Fragments arranged by size:
4442 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3322 4118 6080 7290 3322
Size: 796 1962 1210 4220
Fragments arranged by size:
4220 1962 1210 796
BclI T'GATC_A
Cuts at: 1548 3009 3111 4881 1548
Size: 1461 102 1770 4855
Fragments arranged by size:
4855 1770 1461 102
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 3153 3301
Size: 107 122 71 631 605 406 1075 148
Cuts at: 3301 6799 136
Size: 3498 1525
Fragments arranged by size:
3498 1525 1075 631 605 406 148 122
107 71
BglII A'GATC_T
Cuts at: 8183 8183
Size: 8188
BlpI GC'TnA_GC
Cuts at: 3258 5174 5605 3258
Size: 1916 431 5841
Fragments arranged by size:
5841 1916 431
Bme1580I G_kGCm'C
Cuts at: 1790 2121 3149 3597 5372 6249 7405 1790
Size: 331 1028 448 1775 877 1156 2573
Fragments arranged by size:
2573 1775 1156 1028 877 448 331
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4182 4511 4918
Size: 416 53 83 399 24 2765 329 407
Cuts at: 4918 5919 6877 442
Size: 1001 958 1753
Fragments arranged by size:
2765 1753 1001 958 416 407 399 329
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8188
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 3273 2674
Size: 599 7589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 2934 3020 4316 4392 6848 1239
Size: 1283 412 86 1296 76 2456 2579
Fragments arranged by size:
2579 2456 1296 1283 412 86 76
Bpu10I CC'TnA_GC
Cuts at: 2177 2252 2934 3162 3167 3580 4052 5345 2177
Size: 75 682 228 5 413 472 1293 5020
Fragments arranged by size:
5020 1293 682 472 413 228 75 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4902 6313 7178 7476 4902
Size: 1411 865 298 5614
Fragments arranged by size:
5614 1411 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4816 5086 6851 882
Size: 617 3317 270 1765 2219
Fragments arranged by size:
3317 2219 1765 617 270
BsaAI yAC'GTr
Cuts at: 493 1204 5485 5979 493
Size: 711 4281 494 2702
Fragments arranged by size:
4281 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8182 8182
Size: 8188
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4952 5261 5995 6377
Size: 53 83 186 936 3419 309 734 382
Cuts at: 6377 7012 275
Size: 635 1451
Fragments arranged by size:
3419 1451 936 734 635 382 309 186
83 53
BsaWI w'CCGG_w
Cuts at: 3275 6621 7019 7293 7440 7851 3275
Size: 3346 398 274 147 411 3612
Fragments arranged by size:
3612 3346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2320 2350 2761 2791 3211
Size: 30 165 30 917 30 411 30 420
Cuts at: 3211 3241 4118 4148 4226 4256 4314 4344 1178
Size: 30 877 30 78 30 58 30 5022
Fragments arranged by size:
5022 917 877 420 411 165 78 58
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3085 3150 3409 5049 1186
Size: 1899 65 259 1640 4325
Fragments arranged by size:
4325 1899 1640 259 65
BseYI C'CCAG_C
Cuts at: 3321 3464 3661 4245 5264 7391 7887 3321
Size: 143 197 584 1019 2127 496 3622
Fragments arranged by size:
3622 2127 1019 584 496 197 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4884 1163
Size: 573 3148 4467
Fragments arranged by size:
4467 3148 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5638 6399 7427 1540
Size: 275 186 3637 761 1028 2301
Fragments arranged by size:
3637 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2219 3077 3506 3917 6249 6334 7405 2121
Size: 98 858 429 411 2332 85 1071 2904
Fragments arranged by size:
2904 2332 1071 858 429 411 98 85
BsmI GAATG_Cn'
Cuts at: 3191 4587 5699 5792 3191
Size: 1396 1112 93 5587
Fragments arranged by size:
5587 1396 1112 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2898 3231 2898
Size: 333 7855
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2102 2121 2149 2219 3077 3149
Size: 399 298 14 19 28 70 858 72
Cuts at: 3149 3506 3597 3675 3917 5372 5507 6249 6334
Size: 357 91 78 242 1455 135 742 85
Cuts at: 6334 7405 1391
Size: 1071 2174
Fragments arranged by size:
2174 1455 1071 858 742 399 357 298
242 135 91 85 78 72 70 28
19 14
BspEI T'CCGG_A
Cuts at: 3275 7851 3275
Size: 4576 3612
BspHI T'CATG_A
Cuts at: 6077 7807 6077
Size: 1730 6458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 4993 5543 844
Size: 1598 2551 550 3489
Fragments arranged by size:
3489 2551 1598 550
BsrBI CCG'CTC
Cuts at: 1394 1823 6075 1394
Size: 429 4252 3507
Fragments arranged by size:
4252 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2791 4110 6683 6857 65
Size: 1183 1543 1319 2573 174 1396
Fragments arranged by size:
2573 1543 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3572 6832 7019 1700
Size: 110 1762 3260 187 2869
Fragments arranged by size:
3260 2869 1762 187 110
BsrGI T'GTAC_A
Cuts at: 96 2260 3564 96
Size: 2164 1304 4720
Fragments arranged by size:
4720 2164 1304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8188
BssSI C'ACGA_G
Cuts at: 6248 7260 6248
Size: 1012 7176
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 4342 794
Size: 1640 1908 4640
Fragments arranged by size:
4640 1908 1640
BstBI TT'CG_AA
Cuts at: 2007 6992 2007
Size: 4985 3203
BstEII G'GTnAC_C
Cuts at: 1184 4056 1184
Size: 2872 5316
BstYI r'GATC_y
Cuts at: 1972 2929 3628 4796 5518 5885 6270 6287 7739
Size: 957 699 1168 722 367 385 17 1452
Cuts at: 7739 7825 7837 8183 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1168 957 722 699 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3151 3624 4753 5144 3151
Size: 473 1129 391 6195
Fragments arranged by size:
6195 1129 473 391
BtgI C'CryG_G
Cuts at: 513 1065 2022 3096 3198 3666 4739 5133 513
Size: 552 957 1074 102 468 1073 394 3568
Fragments arranged by size:
3568 1074 1073 957 552 468 394 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7449
Fragments arranged by size:
7449 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 2467 5009 5058 5088 5329 5704 6498 6518
Size: 154 2542 49 30 241 375 794 20
Cuts at: 6518 2313
Size: 3983
Fragments arranged by size:
3983 2542 794 375 241 154 49 30
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2437 2472 529
Size: 35 1873 35 6245
Fragments arranged by size:
6245 1873 35 35
DraI TTT'AAA
Cuts at: 5492 5492
Size: 8188
DraIII CAC_nnn'GTG
Cuts at: 4165 4165
Size: 8188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7195 809
Size: 507 5879 1802
Fragments arranged by size:
5879 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2269 2863 3201
Size: 54 1425 50 275 18 439 594 338
Cuts at: 3201 3535 4742 5635 6524 8153 8
Size: 334 1207 893 889 1629 43
Fragments arranged by size:
1629 1425 1207 893 889 594 439 338
334 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5635 1537
Size: 275 3823 4090
Fragments arranged by size:
4090 3823 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6118 1353
Size: 4765 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6775 7149 7295 387
Size: 1064 138 5186 374 146 1280
Fragments arranged by size:
5186 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2217 3504 3915 2217
Size: 1287 411 6490
Fragments arranged by size:
6490 1287 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2934 3020 3222 4055 4073 4316
Size: 393 890 412 86 202 833 18 243
Cuts at: 4316 4392 4650 6251 6848 7635 7903 1239
Size: 76 258 1601 597 787 268 1524
Fragments arranged by size:
1601 1524 890 833 787 597 412 393
268 258 243 202 86 76 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3267 4752 3267
Size: 1485 6703
EcoO109I rG'GnC_Cy
Cuts at: 2047 2070 2715 3084 3145 3146 3171 4184 5138
Size: 23 645 369 61 1 25 1013 954
Cuts at: 5138 5369 2047
Size: 231 4866
Fragments arranged by size:
4866 1013 954 645 369 231 61 25
23 1
EcoRI G'AATT_C
Cuts at: 2637 5497 5875 2637
Size: 2860 378 4950
Fragments arranged by size:
4950 2860 378
EcoRV GAT'ATC
Cuts at: 2629 2629
Size: 8188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4062 4077 4062
Size: 15 8173
FseI GG_CCGG'CC
Cuts at: 3576 3576
Size: 8188
FspI TGC'GCA
Cuts at: 6694 6694
Size: 8188
HaeII r_GCGC'y
Cuts at: 1678 2039 2140 7335 1678
Size: 361 101 5195 2531
Fragments arranged by size:
5195 2531 361 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2740 2772 2825 2857 4116
Size: 32 135 32 1168 32 53 32 1259
Cuts at: 4116 4138 4145 4148 4170 4177 6829 6861 6903
Size: 22 7 3 22 7 2652 32 42
Cuts at: 6903 6935 1373
Size: 32 2626
Fragments arranged by size:
2652 2626 1259 1168 135 53 42 32
32 32 32 32 32 22 22 7
7 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2569 5073 5532 5778 669
Size: 988 281 631 2504 459 246 3079
Fragments arranged by size:
3079 2504 988 631 459 281 246
HindIII A'AGCT_T
Cuts at: 748 4486 748
Size: 3738 4450
HpaI GTT'AAC
Cuts at: 2569 5073 5778 2569
Size: 2504 705 4979
Fragments arranged by size:
4979 2504 705
KpnI G_GTAC'C
Cuts at: 5513 5513
Size: 8188
MfeI C'AATT_G
Cuts at: 4268 4679 4706 4817 5787 4268
Size: 411 27 111 970 6669
Fragments arranged by size:
6669 970 411 111 27
MluI A'CGCG_T
Cuts at: 8064 8064
Size: 8188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 7302 7486 1305
Size: 3609 2388 184 2007
Fragments arranged by size:
3609 2388 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2271 2865 3203 10
Size: 54 1425 343 439 594 338 4995
Fragments arranged by size:
4995 1425 594 439 343 338 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2859 2972 3600 5322 6146 6505 6664
Size: 648 1693 113 628 1722 824 359 159
Cuts at: 6664 518
Size: 2042
Fragments arranged by size:
2042 1722 1693 824 648 628 359 159
113
MspA1I CmG'CkG
Cuts at: 1606 3166 3182 3534 5604 6281 7429 7674 1606
Size: 1560 16 352 2070 677 1148 245 2120
Fragments arranged by size:
2120 2070 1560 1148 677 352 245 16
NaeI GCC'GGC
Cuts at: 3574 3574
Size: 8188
NcoI C'CATG_G
Cuts at: 513 1065 2022 3096 3198 3666 5133 513
Size: 552 957 1074 102 468 1467 3568
Fragments arranged by size:
3568 1467 1074 957 552 468 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8188
NgoMIV G'CCGG_C
Cuts at: 3572 3572
Size: 8188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8188
NotI GC'GGCC_GC
Cuts at: 5635 5635
Size: 8188
NspI r_CATG'y
Cuts at: 1132 1229 3362 3565 4855 5546 7091 1132
Size: 97 2133 203 1290 691 1545 2229
Fragments arranged by size:
2229 2133 1545 1290 691 203 97
PasI CC'CwG_GG
Cuts at: 1348 2504 3586 1348
Size: 1156 1082 5950
Fragments arranged by size:
5950 1156 1082
PciI A'CATG_T
Cuts at: 3561 7087 3561
Size: 3526 4662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3182 4223 4507 4843 1108
Size: 2074 1041 284 336 4453
Fragments arranged by size:
4453 2074 1041 336 284
PmeI GTTT'AAAC
Cuts at: 5492 5492
Size: 8188
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6257 6289 7770 7802 6257
Size: 32 1481 32 6643
Fragments arranged by size:
6643 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2715 5138 2715
Size: 2423 5765
PshAI GACnn'nnGTC
Cuts at: 1613 3054 4393 1613
Size: 1441 1339 5408
Fragments arranged by size:
5408 1441 1339
PsiI TTA'TAA
Cuts at: 4416 5758 4416
Size: 1342 6846
PspOMI G'GGCC_C
Cuts at: 3145 5368 3145
Size: 2223 5965
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5465 5497 5465
Size: 32 8156
PstI C_TGCA'G
Cuts at: 830 2665 2943 4960 4990 5540 830
Size: 1835 278 2017 30 550 3478
Fragments arranged by size:
3478 2017 1835 550 278 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8188
PvuII CAG'CTG
Cuts at: 3166 3182 3166
Size: 16 8172
SacI G_AGCT'C
Cuts at: 2219 3506 3917 2219
Size: 1287 411 6490
Fragments arranged by size:
6490 1287 411
SalI G'TCGA_C
Cuts at: 1936 5530 1936
Size: 3594 4594
SanDI GG'GwC_CC
Cuts at: 5138 5138
Size: 8188
SbfI CC_TGCA'GG
Cuts at: 5540 5540
Size: 8188
ScaI AGT'ACT
Cuts at: 6436 8148 6436
Size: 1712 6476
SexAI A'CCwGG_T
Cuts at: 3436 3436
Size: 8188
SfcI C'TryA_G
Cuts at: 826 1045 2328 2661 2939 4292 4596 4956 4986
Size: 219 1283 333 278 1353 304 360 30
Cuts at: 4986 5536 6671 7352 7543 826
Size: 550 1135 681 191 1471
Fragments arranged by size:
1471 1353 1283 1135 681 550 360 333
304 278 219 191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8188
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8188
SmaI CCC'GGG
Cuts at: 1872 5515 1872
Size: 3643 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4917 6292 7193 7455 820
Size: 197 926 2974 1375 901 262 1553
Fragments arranged by size:
2974 1553 1375 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5485 5979 493
Size: 4992 494 2702
Fragments arranged by size:
4992 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8188
SphI G_CATG'C
Cuts at: 1132 3362 4855 5546 1132
Size: 2230 1493 691 3774
Fragments arranged by size:
3774 2230 1493 691
SspI AAT'ATT
Cuts at: 5 52 2884 6112 5
Size: 47 2832 3228 2081
Fragments arranged by size:
3228 2832 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8188
StyI C'CwwG_G
Cuts at: 513 1065 2022 2089 2301 3080 3096 3198 3666
Size: 552 957 67 212 779 16 102 468
Cuts at: 3666 5133 5627 513
Size: 1467 494 3074
Fragments arranged by size:
3074 1467 957 779 552 494 468 212
102 67 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3675 3797 5296 6215 6232 6385 6570
Size: 1255 2095 122 1499 919 17 153 185
Cuts at: 6570 7015 325
Size: 445 1498
Fragments arranged by size:
2095 1499 1498 1255 919 445 185 153
122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2260 3564 6434
Size: 274 80 33 51 1443 283 1304 2870
Cuts at: 6434 8146 96
Size: 1712 138
Fragments arranged by size:
2870 1712 1443 1304 283 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3211 3243 8148 8180 3211
Size: 32 4905 32 3219
Fragments arranged by size:
4905 3219 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2697 2839 1316
Size: 1381 142 6665
Fragments arranged by size:
6665 1381 142
XbaI T'CTAG_A
Cuts at: 5524 5524
Size: 8188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2687 4117 5120 2605
Size: 82 1430 1003 5673
Fragments arranged by size:
5673 1430 1003 82
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8188
XmaI C'CCGG_G
Cuts at: 1870 5513 1870
Size: 3643 4545
XmnI GAAnn'nnTTC
Cuts at: 6317 6317
Size: 8188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4953 5996 276
Size: 53 83 186 936 3419 1043 2468
Fragments arranged by size:
3419 2468 1043 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsiWI BstXI
BstZ17I FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI RsrII SacII SapI SfiI SfoI SrfI
SwaI