Restriction map for
pFN21ASDA1809
(Circular) (Six-base) MAPSORT of: pFN21ASDA1809.seq Check: 8294 from: 1 to: 7048
pFN21ASDA1809 7048 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3660 4858 278
Size: 53 83 186 936 2124 1198 2468
Fragments arranged by size:
2468 2124 1198 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4391 1937
Size: 2454 4594
Acc65I G'GTAC_C
Cuts at: 4369 4369
Size: 7048
AclI AA'CG_TT
Cuts at: 1563 5175 5548 1563
Size: 3612 373 3063
Fragments arranged by size:
3612 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2649 3302 3679 5111 6495 6763 1632
Size: 1017 653 377 1432 1384 268 1917
Fragments arranged by size:
1917 1432 1384 1017 653 377 268
AflII C'TTAA_G
Cuts at: 820 1017 2056 4334 820
Size: 197 1039 2278 3534
Fragments arranged by size:
3534 2278 1039 197
AflIII A'CryG_T
Cuts at: 1203 2534 2997 3258 3984 5947 6924 1203
Size: 1331 463 261 726 1963 977 1327
Fragments arranged by size:
1963 1331 1327 977 726 463 261
AgeI A'CCGG_T
Cuts at: 5879 5879
Size: 7048
AhdI GACnn_n'nnGTC
Cuts at: 5777 5777
Size: 7048
AleI CACnn'nnGTG
Cuts at: 1166 2904 1166
Size: 1738 5310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7048
AlwNI CAG_nnn'CTG
Cuts at: 2247 3718 4791 6363 2247
Size: 1471 1073 1572 2932
Fragments arranged by size:
2932 1572 1471 1073
ApaI G_GGCC'C
Cuts at: 4211 4211
Size: 7048
ApaLI G'TGCA_C
Cuts at: 3203 3262 5105 6261 3203
Size: 59 1843 1156 3990
Fragments arranged by size:
3990 1843 1156 59
ApoI r'AATT_y
Cuts at: 1493 2069 2379 3182 4357 4583 4735 6844 1493
Size: 576 310 803 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 803 576 310 226 152
AscI GG'CGCG_CC
Cuts at: 3587 3587
Size: 7048
AseI AT'TA_AT
Cuts at: 160 1032 5602 160
Size: 872 4570 1606
Fragments arranged by size:
4570 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2902 3847 3862 3935 4130 4153
Size: 73 135 824 945 15 73 195 23
Cuts at: 4153 4210 4324 4373 1870
Size: 57 114 49 4545
Fragments arranged by size:
4545 945 824 195 135 114 73 73
57 49 23 15
BamHI G'GATC_C
Cuts at: 4378 4745 4378
Size: 367 6681
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3374 4369 5824 618
Size: 325 217 627 1587 995 1455 1842
Fragments arranged by size:
1842 1587 1455 995 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2137 2461 2523 3914 4211 4294 4333 4367
Size: 746 324 62 1391 297 83 39 34
Cuts at: 4367 1391
Size: 4072
Fragments arranged by size:
4072 1391 746 324 297 83 62 39
34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3302 3789 928
Size: 971 1403 487 4187
Fragments arranged by size:
4187 1403 971 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 5239 5273 1903
Size: 34 1979 34 1289 34 3678
Fragments arranged by size:
3678 1979 1289 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2669 4940 6150 2669
Size: 2271 1210 3567
Fragments arranged by size:
3567 2271 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7048
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3222 5659 136
Size: 107 122 71 631 605 1550 2437 1525
Fragments arranged by size:
2437 1550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7043 7043
Size: 7048
BlpI GC'TnA_GC
Cuts at: 3572 3677 3908 4465 3572
Size: 105 231 557 6155
Fragments arranged by size:
6155 557 231 105
Bme1580I G_kGCm'C
Cuts at: 1790 3207 3266 4211 5109 6265 1790
Size: 1417 59 945 898 1156 2573
Fragments arranged by size:
2573 1417 1156 945 898 59
BmgBI CAC'GTC
Cuts at: 1318 3000 1318
Size: 1682 5366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4779 5737 442
Size: 416 53 83 399 24 3362 958 1753
Fragments arranged by size:
3362 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3622 3877 4111 3622
Size: 255 234 6559
Fragments arranged by size:
6559 255 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 2443 3599 4003 4042 5708 1239
Size: 882 322 1156 404 39 1666 2579
Fragments arranged by size:
2579 1666 1156 882 404 322 39
Bpu10I CC'TnA_GC
Cuts at: 4043 4043
Size: 7048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3395 4278 5173 6038 6336 3395
Size: 883 895 865 298 4107
Fragments arranged by size:
4107 895 883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3350 5711 882
Size: 617 1851 2361 2219
Fragments arranged by size:
2361 2219 1851 617
BsaAI yAC'GTr
Cuts at: 493 1204 4345 4839 493
Size: 711 3141 494 2702
Fragments arranged by size:
3141 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2761 7042 2761
Size: 4281 2767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3092 3657 4855 5237
Size: 53 83 186 936 1559 565 1198 382
Cuts at: 5237 5872 275
Size: 635 1451
Fragments arranged by size:
1559 1451 1198 936 635 565 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5481 5879 6153 6300 6711 5481
Size: 398 274 147 411 5818
Fragments arranged by size:
5818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3332 3362 3971 4001 4031
Size: 30 165 30 1929 30 609 30 30
Cuts at: 4031 1178
Size: 4195
Fragments arranged by size:
4195 1929 609 165 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2425 3096 3114 3491 3500 3527 3536
Size: 961 278 671 18 377 9 27 9
Cuts at: 3536 3539 3542 3545 4069 4084 1186
Size: 3 3 3 524 15 4150
Fragments arranged by size:
4150 961 671 524 377 278 27 18
15 9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2049 2522 2691 3029 3132 3889 3899 4220 6251
Size: 473 169 338 103 757 10 321 2031
Cuts at: 6251 6747 2049
Size: 496 2350
Fragments arranged by size:
2350 2031 757 496 473 338 321 169
103 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2433 2505 2697 1163
Size: 573 666 31 72 192 5514
Fragments arranged by size:
5514 666 573 192 72 31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3621 3963 4498 5259 6287 1540
Size: 275 186 1620 342 535 761 1028 2301
Fragments arranged by size:
2301 1620 1028 761 535 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 2137 2220 2461 3207 3266 3678 4092 4187 4294
Size: 83 241 746 59 412 414 95 107
Cuts at: 4294 5109 5194 6265 2137
Size: 815 85 1071 2920
Fragments arranged by size:
2920 1071 815 746 414 412 241 107
95 85 83 59
BsmI GAATG_Cn'
Cuts at: 4559 4652 4559
Size: 93 6955
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2220 2461 2523 3207 3266 3678
Size: 399 347 83 241 62 684 59 412
Cuts at: 3678 3914 4092 4187 4211 4294 4333 4367 5109
Size: 236 178 95 24 83 39 34 742
Cuts at: 5109 5194 6265 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 412 399 347 241
236 178 95 85 83 83 62 59
39 34 24
BspEI T'CCGG_A
Cuts at: 6711 6711
Size: 7048
BspHI T'CATG_A
Cuts at: 2149 4937 6667 2149
Size: 2788 1730 2530
Fragments arranged by size:
2788 2530 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2748 3960 4403 844
Size: 1904 1212 443 3489
Fragments arranged by size:
3489 1904 1212 443
BsrBI CCG'CTC
Cuts at: 1394 1823 4935 1394
Size: 429 3112 3507
Fragments arranged by size:
3507 3112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 5543 5717 65
Size: 1183 994 3301 174 1396
Fragments arranged by size:
3301 1396 1183 994 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3024 3207 3794 4004 5692 5879 1700
Size: 110 1214 183 587 210 1688 187 2869
Fragments arranged by size:
2869 1688 1214 587 210 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 2537 96
Size: 2441 4607
BssHII G'CGCG_C
Cuts at: 1925 3405 3587 1925
Size: 1480 182 5386
Fragments arranged by size:
5386 1480 182
BssSI C'ACGA_G
Cuts at: 3596 5108 6120 3596
Size: 1512 1012 4524
Fragments arranged by size:
4524 1512 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2247 2492 3249 794
Size: 1453 245 757 4593
Fragments arranged by size:
4593 1453 757 245
BstBI TT'CG_AA
Cuts at: 2007 5852 2007
Size: 3845 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7048
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 3385 4199 2317
Size: 1068 814 5166
Fragments arranged by size:
5166 1068 814
BstYI r'GATC_y
Cuts at: 1972 4033 4149 4378 4745 5130 5147 6599 6685
Size: 2061 116 229 367 385 17 1452 86
Cuts at: 6685 6697 7043 1972
Size: 12 346 1977
Fragments arranged by size:
2061 1977 1452 385 367 346 229 116
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2119 2316 2648 2704 2958 3417 3835
Size: 552 1054 197 332 56 254 459 418
Cuts at: 3835 513
Size: 3726
Fragments arranged by size:
3726 1054 552 459 418 332 254 197
56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3980 487
Size: 683 56 2754 3555
Fragments arranged by size:
3555 2754 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 4001 4564 5358 5378 2177
Size: 1824 563 794 20 3847
Fragments arranged by size:
3847 1824 794 563 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7048
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4283 4318 529
Size: 35 3719 35 3259
Fragments arranged by size:
3719 3259 35 35
DraI TTT'AAA
Cuts at: 4352 4352
Size: 7048
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3331 6055 809
Size: 507 2015 2724 1802
Fragments arranged by size:
2724 2015 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2889 3423 3739
Size: 54 1425 50 275 18 1059 534 316
Cuts at: 3739 3747 3792 3832 3960 4495 5384 7013 8
Size: 8 45 40 128 535 889 1629 43
Fragments arranged by size:
1629 1425 1059 889 535 534 316 275
128 54 50 45 43 40 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 3960 4495 1537
Size: 275 2148 535 4090
Fragments arranged by size:
4090 2148 535 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 3477 3495 3504 3513 4978 1353
Size: 1518 606 18 9 9 1465 3423
Fragments arranged by size:
3423 1518 1465 606 18 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2888 3106 5635 6009 6155 387
Size: 1064 138 1299 218 2529 374 146 1280
Fragments arranged by size:
2529 1299 1280 1064 374 218 146 138
EcoICRI GAG'CTC
Cuts at: 2135 2459 4292 2135
Size: 324 1833 4891
Fragments arranged by size:
4891 1833 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2443 2649 3302 3599 3679 4003
Size: 393 489 322 206 653 297 80 324
Cuts at: 4003 4042 5111 5708 6495 6763 1239
Size: 39 1069 597 787 268 1524
Fragments arranged by size:
1524 1069 787 653 597 489 393 324
322 297 268 206 80 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2914 3749 2914
Size: 835 6213
EcoO109I rG'GnC_Cy
Cuts at: 2428 3715 3868 2428
Size: 1287 153 5608
Fragments arranged by size:
5608 1287 153
EcoRI G'AATT_C
Cuts at: 2069 4357 4735 2069
Size: 2288 378 4382
Fragments arranged by size:
4382 2288 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 3097 2114
Size: 983 6065
FseI GG_CCGG'CC
Cuts at: 3028 3028
Size: 7048
FspI TGC'GCA
Cuts at: 5554 5554
Size: 7048
HaeII r_GCGC'y
Cuts at: 1678 2797 2866 3289 3399 6195 1678
Size: 1119 69 423 110 2796 2531
Fragments arranged by size:
2796 2531 1119 423 110 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2448 2480 2595
Size: 32 135 32 773 32 71 32 115
Cuts at: 2595 2627 3332 3364 3770 3802 4307 4339 5689
Size: 32 705 32 406 32 505 32 1350
Cuts at: 5689 5721 5763 5795 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 773 705 505 406 135 115
71 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4392 4638 669
Size: 988 281 2454 246 3079
Fragments arranged by size:
3079 2454 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7048
HpaI GTT'AAC
Cuts at: 4638 4638
Size: 7048
KpnI G_GTAC'C
Cuts at: 4373 4373
Size: 7048
MfeI C'AATT_G
Cuts at: 4647 4647
Size: 7048
MluI A'CGCG_T
Cuts at: 3258 6924 3258
Size: 3666 3382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3360 3783 4065 6162 6346 1305
Size: 2055 423 282 2097 184 2007
Fragments arranged by size:
2097 2055 2007 423 282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2891 3425 10
Size: 54 1425 343 1059 534 3633
Fragments arranged by size:
3633 1425 1059 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2904 4197 5006 5365 5524 518
Size: 648 1738 1293 809 359 159 2042
Fragments arranged by size:
2042 1738 1293 809 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2650 3013 3837 4164 4464 5141 6289 6534
Size: 1044 363 824 327 300 677 1148 245
Cuts at: 6534 1606
Size: 2120
Fragments arranged by size:
2120 1148 1044 824 677 363 327 300
245
NaeI GCC'GGC
Cuts at: 3026 3796 4006 3026
Size: 770 210 6068
Fragments arranged by size:
6068 770 210
NcoI C'CATG_G
Cuts at: 513 1065 2704 2958 513
Size: 552 1639 254 4603
Fragments arranged by size:
4603 1639 552 254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7048
NgoMIV G'CCGG_C
Cuts at: 3024 3794 4004 3024
Size: 770 210 6068
Fragments arranged by size:
6068 770 210
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7048
NotI GC'GGCC_GC
Cuts at: 4495 4495
Size: 7048
NspI r_CATG'y
Cuts at: 1132 1229 2538 3307 4406 5951 1132
Size: 97 1309 769 1099 1545 2229
Fragments arranged by size:
2229 1545 1309 1099 769 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7048
PciI A'CATG_T
Cuts at: 2534 5947 2534
Size: 3413 3635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2568 3718 1108
Size: 1460 1150 4438
Fragments arranged by size:
4438 1460 1150
PfoI T'CCnGG_A
Cuts at: 3459 3459
Size: 7048
PmeI GTTT'AAAC
Cuts at: 4352 4352
Size: 7048
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 3929 3961 3974 4006 5117 5149 6630
Size: 32 1886 32 13 32 1111 32 1481
Cuts at: 6630 6662 2011
Size: 32 2397
Fragments arranged by size:
2397 1886 1481 1111 32 32 32 32
32 13
PpuMI rG'GwC_Cy
Cuts at: 3715 3715
Size: 7048
PshAI GACnn'nnGTC
Cuts at: 1613 3342 1613
Size: 1729 5319
PsiI TTA'TAA
Cuts at: 4618 4618
Size: 7048
PspOMI G'GGCC_C
Cuts at: 4207 4207
Size: 7048
PstI C_TGCA'G
Cuts at: 830 4400 830
Size: 3570 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7048
RsrII CG'GwC_CG
Cuts at: 3805 3805
Size: 7048
SacI G_AGCT'C
Cuts at: 2137 2461 4294 2137
Size: 324 1833 4891
Fragments arranged by size:
4891 1833 324
SacII CC_GC'GG
Cuts at: 2651 3838 2651
Size: 1187 5861
SalI G'TCGA_C
Cuts at: 1936 4390 1936
Size: 2454 4594
SbfI CC_TGCA'GG
Cuts at: 4400 4400
Size: 7048
ScaI AGT'ACT
Cuts at: 5296 7008 5296
Size: 1712 5336
SexAI A'CCwGG_T
Cuts at: 3195 3711 3195
Size: 516 6532
SfcI C'TryA_G
Cuts at: 826 1045 4396 5531 6212 6403 826
Size: 219 3351 1135 681 191 1471
Fragments arranged by size:
3351 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7048
SgrAI Cr'CCGG_yG
Cuts at: 1700 3207 1700
Size: 1507 5541
SmaI CCC'GGG
Cuts at: 1872 3849 4132 4212 4375 1872
Size: 1977 283 80 163 4545
Fragments arranged by size:
4545 1977 283 163 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 3410 3935 4293 4324 4334
Size: 197 926 113 1354 525 358 31 10
Cuts at: 4334 5152 6053 6315 820
Size: 818 901 262 1553
Fragments arranged by size:
1553 1354 926 901 818 525 358 262
197 113 31 10
SnaBI TAC'GTA
Cuts at: 493 4345 4839 493
Size: 3852 494 2702
Fragments arranged by size:
3852 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7048
SphI G_CATG'C
Cuts at: 1132 4406 1132
Size: 3274 3774
SrfI GCCC'GGGC
Cuts at: 4212 4212
Size: 7048
SspI AAT'ATT
Cuts at: 5 52 4972 5
Size: 47 4920 2081
Fragments arranged by size:
4920 2081 47
StuI AGG'CCT
Cuts at: 1517 3704 1517
Size: 2187 4861
StyI C'CwwG_G
Cuts at: 513 1065 2704 2958 4487 513
Size: 552 1639 254 1529 3074
Fragments arranged by size:
3074 1639 1529 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2818 2909 3281 5075 5092 5245 5430
Size: 1255 1238 91 372 1794 17 153 185
Cuts at: 5430 5875 325
Size: 445 1498
Fragments arranged by size:
1794 1498 1255 1238 445 372 185 153
91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2537 3987 5294
Size: 274 80 33 51 1443 560 1450 1307
Cuts at: 5294 7006 96
Size: 1712 138
Fragments arranged by size:
1712 1450 1443 1307 560 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 7008 7040 3299
Size: 32 3677 32 3307
Fragments arranged by size:
3677 3307 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7048
XbaI T'CTAG_A
Cuts at: 2372 4384 2372
Size: 2012 5036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3385 3385
Size: 7048
XhoI C'TCGA_G
Cuts at: 1943 3935 4324 1943
Size: 1992 389 4667
Fragments arranged by size:
4667 1992 389
XmaI C'CCGG_G
Cuts at: 1870 3847 4130 4210 4373 1870
Size: 1977 283 80 163 4545
Fragments arranged by size:
4545 1977 283 163 80
XmnI GAAnn'nnTTC
Cuts at: 2094 3944 5177 2094
Size: 1850 1233 3965
Fragments arranged by size:
3965 1850 1233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3658 4856 276
Size: 53 83 186 936 2124 1198 2468
Fragments arranged by size:
2468 2124 1198 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI PvuII
SanDI SapI SfiI SfoI SwaI