Restriction map for
pFN21ASDA1798
(Circular) (Six-base) MAPSORT of: pFN21ASDA1798.seq Check: 7437 from: 1 to: 7069
pFN21ASDA1798 7069 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 2284
Size: 7069
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4879 278
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3162 3474 4412 1937
Size: 1225 312 938 4594
Fragments arranged by size:
4594 1225 938 312
Acc65I G'GTAC_C
Cuts at: 2218 4390 2218
Size: 2172 4897
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3626 3663 5132 6516 6784 1632
Size: 725 1269 37 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 1269 725 268 37
AflII C'TTAA_G
Cuts at: 820 1017 3059 3820 820
Size: 197 2042 761 4069
Fragments arranged by size:
4069 2042 761 197
AflIII A'CryG_T
Cuts at: 1203 3065 5968 6945 1203
Size: 1862 2903 977 1327
Fragments arranged by size:
2903 1862 1327 977
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 4159 5798 4159
Size: 1639 5430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7069
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3993 4025 3993
Size: 32 7037
AlwNI CAG_nnn'CTG
Cuts at: 4812 6384 4812
Size: 1572 5497
ApaI G_GGCC'C
Cuts at: 3752 4295 3752
Size: 543 6526
ApaLI G'TGCA_C
Cuts at: 5126 6282 5126
Size: 1156 5913
ApoI r'AATT_y
Cuts at: 1493 3038 4166 4305 4345 4378 4604 4756 6865
Size: 1545 1128 139 40 33 226 152 2109
Cuts at: 6865 1493
Size: 1697
Fragments arranged by size:
2109 1697 1545 1128 226 152 139 40
33
AseI AT'TA_AT
Cuts at: 160 1032 3668 5623 160
Size: 872 2636 1955 1606
Fragments arranged by size:
2636 1955 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2846 3703 3993 4394 1870
Size: 73 308 595 857 290 401 4545
Fragments arranged by size:
4545 857 595 401 308 290 73
BamHI G'GATC_C
Cuts at: 2350 2842 4399 4766 2350
Size: 492 1557 367 4653
Fragments arranged by size:
4653 1557 492 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 3989 4390 5845 618
Size: 325 217 627 431 1771 401 1455 1842
Fragments arranged by size:
1842 1771 1455 627 431 401 325 217
BanII G_rGCy'C
Cuts at: 1391 2149 2258 3659 3752 4295 4388 1391
Size: 758 109 1401 93 543 93 4072
Fragments arranged by size:
4072 1401 758 543 109 93 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 2508 3942 928
Size: 971 431 178 1434 4055
Fragments arranged by size:
4055 1434 971 431 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5260 5294 1903
Size: 34 3323 34 3678
Fragments arranged by size:
3678 3323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 4961 6171 2888
Size: 2073 1210 3786
Fragments arranged by size:
3786 2073 1210
BclI T'GATC_A
Cuts at: 1548 3526 1548
Size: 1978 5091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5680 136
Size: 107 122 71 631 605 4008 1525
Fragments arranged by size:
4008 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7064 7064
Size: 7069
BlpI GC'TnA_GC
Cuts at: 4486 4486
Size: 7069
Bme1580I G_kGCm'C
Cuts at: 1790 2102 3752 3994 4295 5130 6286 1790
Size: 312 1650 242 301 835 1156 2573
Fragments arranged by size:
2573 1650 1156 835 312 301 242
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7069
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2337 2969 3253
Size: 416 53 83 399 24 920 632 284
Cuts at: 3253 4298 4800 5758 442
Size: 1045 502 958 1753
Fragments arranged by size:
1753 1045 958 920 632 502 416 399
284 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2242 2445 5729 1239
Size: 1003 203 3284 2579
Fragments arranged by size:
3284 2579 1003 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2214 3060 3173 3485 5194 6059 6357 2214
Size: 846 113 312 1709 865 298 2926
Fragments arranged by size:
2926 1709 865 846 312 298 113
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3903 5732 882
Size: 617 2404 1829 2219
Fragments arranged by size:
2404 2219 1829 617
BsaAI yAC'GTr
Cuts at: 493 1204 4366 4860 493
Size: 711 3162 494 2702
Fragments arranged by size:
3162 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2472 7063 2472
Size: 4591 2478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4876 5258 5893 275
Size: 53 83 186 936 3343 382 635 1451
Fragments arranged by size:
3343 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2898 5502 5900 6174 6321 6732 2898
Size: 2604 398 274 147 411 3235
Fragments arranged by size:
3235 2604 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6844
Fragments arranged by size:
6844 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 3426 1186
Size: 1570 670 4829
Fragments arranged by size:
4829 1570 670
BseYI C'CCAG_C
Cuts at: 2721 4080 6272 6768 2721
Size: 1359 2192 496 3022
Fragments arranged by size:
3022 2192 1359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4519 5280 6308 1540
Size: 275 186 2518 761 1028 2301
Fragments arranged by size:
2518 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 2455 2650 4151 5130 5215 6286 2258
Size: 197 195 1501 979 85 1071 3041
Fragments arranged by size:
3041 1501 1071 979 197 195 85
BsmI GAATG_Cn'
Cuts at: 2956 4580 4673 2956
Size: 1624 93 5352
Fragments arranged by size:
5352 1624 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2149 2258 2455 2650 3659 3752
Size: 399 312 47 109 197 195 1009 93
Cuts at: 3752 3994 4151 4295 4388 5130 5215 6286 1391
Size: 242 157 144 93 742 85 1071 2174
Fragments arranged by size:
2174 1071 1009 742 399 312 242 197
195 157 144 109 93 93 85 47
BspEI T'CCGG_A
Cuts at: 6732 6732
Size: 7069
BspHI T'CATG_A
Cuts at: 4958 6688 4958
Size: 1730 5339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2284 3412 4424 844
Size: 1440 1128 1012 3489
Fragments arranged by size:
3489 1440 1128 1012
BsrBI CCG'CTC
Cuts at: 1394 1823 4956 1394
Size: 429 3133 3507
Fragments arranged by size:
3507 3133 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5564 5738 65
Size: 1183 4316 174 1396
Fragments arranged by size:
4316 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2761 2996 5713 5900 1700
Size: 110 951 235 2717 187 2869
Fragments arranged by size:
2869 2717 951 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7069
BssSI C'ACGA_G
Cuts at: 5129 6141 5129
Size: 1012 6057
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2692 794
Size: 1898 5171
BstBI TT'CG_AA
Cuts at: 2007 5873 2007
Size: 3866 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7069
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2978 2982 3303 2215
Size: 763 4 321 5981
Fragments arranged by size:
5981 763 321 4
BstYI r'GATC_y
Cuts at: 1972 2350 2518 2842 4399 4766 5151 5168 6620
Size: 378 168 324 1557 367 385 17 1452
Cuts at: 6620 6706 6718 7064 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1557 1452 385 378 367 346 324
168 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2630 2630
Size: 7069
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6517
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6330
Fragments arranged by size:
6330 683 56
BtsI GCAGTG_nn'
Cuts at: 2763 4585 5379 5399 2763
Size: 1822 794 20 4433
Fragments arranged by size:
4433 1822 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7034
DraI TTT'AAA
Cuts at: 4213 4373 4213
Size: 160 6909
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6076 809
Size: 507 4760 1802
Fragments arranged by size:
4760 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2998 3721 4516
Size: 54 1425 50 275 18 1168 723 795
Cuts at: 4516 5405 7034 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1168 889 795 723 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4516 1537
Size: 275 2704 4090
Fragments arranged by size:
4090 2704 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2580 2733 3895 3939 4999 1353
Size: 1227 153 1162 44 1060 3423
Fragments arranged by size:
3423 1227 1162 1060 153 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5656 6030 6176 387
Size: 1064 138 4067 374 146 1280
Fragments arranged by size:
4067 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2256 2256
Size: 7069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 2357 2445 3626 3663 5132 5729
Size: 393 610 115 88 1181 37 1469 597
Cuts at: 5729 6516 6784 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1469 1181 787 610 597 393 268
115 88 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2700 2738 3018 2700
Size: 38 280 6751
Fragments arranged by size:
6751 280 38
EcoO109I rG'GnC_Cy
Cuts at: 3749 4292 3749
Size: 543 6526
EcoRI G'AATT_C
Cuts at: 3038 4345 4378 4756 3038
Size: 1307 33 378 5351
Fragments arranged by size:
5351 1307 378 33
EcoRV GAT'ATC
Cuts at: 2968 2968
Size: 7069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 3909 2929
Size: 980 6089
FspI TGC'GCA
Cuts at: 5575 5575
Size: 7069
HaeII r_GCGC'y
Cuts at: 1678 6216 1678
Size: 4538 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2771 2803 2840 2872 4145
Size: 32 135 32 1199 32 37 32 1273
Cuts at: 4145 4177 5710 5742 5784 5816 1373
Size: 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 1273 1199 135 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4413 4659 669
Size: 988 281 2475 246 3079
Fragments arranged by size:
3079 2475 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2906 3149 3461 3675 748
Size: 2158 243 312 214 4142
Fragments arranged by size:
4142 2158 312 243 214
HpaI GTT'AAC
Cuts at: 4659 4659
Size: 7069
KpnI G_GTAC'C
Cuts at: 2222 4394 2222
Size: 2172 4897
MfeI C'AATT_G
Cuts at: 3043 3562 4668 3043
Size: 519 1106 5444
Fragments arranged by size:
5444 1106 519
MluI A'CGCG_T
Cuts at: 6945 6945
Size: 7069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2513 4130 6183 6367 1305
Size: 1208 1617 2053 184 2007
Fragments arranged by size:
2053 2007 1617 1208 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3723 10
Size: 54 1425 343 1891 3356
Fragments arranged by size:
3356 1891 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2299 2916 3549 3921 4322 5027
Size: 648 1047 86 617 633 372 401 705
Cuts at: 5027 5386 5545 518
Size: 359 159 2042
Fragments arranged by size:
2042 1047 705 648 633 617 401 372
359 159 86
MspA1I CmG'CkG
Cuts at: 1606 4034 4485 5162 6310 6555 1606
Size: 2428 451 677 1148 245 2120
Fragments arranged by size:
2428 2120 1148 677 451 245
NaeI GCC'GGC
Cuts at: 2763 2763
Size: 7069
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6517
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NgoMIV G'CCGG_C
Cuts at: 2761 2761
Size: 7069
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7069
NotI GC'GGCC_GC
Cuts at: 4516 4516
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 3069 3920 4427 5972 1132
Size: 97 1840 851 507 1545 2229
Fragments arranged by size:
2229 1840 1545 851 507 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7069
PciI A'CATG_T
Cuts at: 3065 5968 3065
Size: 2903 4166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2211 1108
Size: 1103 5966
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5138 5170 6651 6683 5138
Size: 32 1481 32 5524
Fragments arranged by size:
5524 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PspOMI G'GGCC_C
Cuts at: 3748 4291 3748
Size: 543 6526
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3316 3348 3316
Size: 32 7037
PstI C_TGCA'G
Cuts at: 830 4339 4421 830
Size: 3509 82 3478
Fragments arranged by size:
3509 3478 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 4034 4034
Size: 7069
SacI G_AGCT'C
Cuts at: 2258 2258
Size: 7069
SalI G'TCGA_C
Cuts at: 1936 4411 1936
Size: 2475 4594
SapI GCTCTTCn'nnn_
Cuts at: 3895 3895
Size: 7069
SbfI CC_TGCA'GG
Cuts at: 4421 4421
Size: 7069
ScaI AGT'ACT
Cuts at: 5317 7029 5317
Size: 1712 5357
SexAI A'CCwGG_T
Cuts at: 3340 3340
Size: 7069
SfcI C'TryA_G
Cuts at: 826 1045 3609 4335 4417 5552 6233 6424 826
Size: 219 2564 726 82 1135 681 191 1471
Fragments arranged by size:
2564 1471 1135 726 681 219 191 82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7069
SmaI CCC'GGG
Cuts at: 1872 3705 3995 4396 1872
Size: 1833 290 401 4545
Fragments arranged by size:
4545 1833 401 290
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2229 3059 3075 3152 3464 3820
Size: 197 926 286 830 16 77 312 356
Cuts at: 3820 5173 6074 6336 820
Size: 1353 901 262 1553
Fragments arranged by size:
1553 1353 926 901 830 356 312 286
262 197 77 16
SnaBI TAC'GTA
Cuts at: 493 4366 4860 493
Size: 3873 494 2702
Fragments arranged by size:
3873 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 3920 4427 1132
Size: 2788 507 3774
Fragments arranged by size:
3774 2788 507
SspI AAT'ATT
Cuts at: 5 52 4993 5
Size: 47 4941 2081
Fragments arranged by size:
4941 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7069
StyI C'CwwG_G
Cuts at: 513 1065 4508 513
Size: 552 3443 3074
Fragments arranged by size:
3443 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2935 2970 2988 4017 4176 5096 5113
Size: 1255 1355 35 18 1029 159 920 17
Cuts at: 5113 5266 5451 5896 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1355 1255 1029 920 445 185 159
153 35 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2677 2862 5315
Size: 274 80 33 51 1443 700 185 2453
Cuts at: 5315 7027 96
Size: 1712 138
Fragments arranged by size:
2453 1712 1443 700 274 185 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7029 7061 7029
Size: 32 7037
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7069
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2280 2212
Size: 68 7001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7069
XmaI C'CCGG_G
Cuts at: 1870 3703 3993 4394 1870
Size: 1833 290 401 4545
Fragments arranged by size:
4545 1833 401 290
XmnI GAAnn'nnTTC
Cuts at: 2370 2771 4239 5198 2370
Size: 401 1468 959 4241
Fragments arranged by size:
4241 1468 959 401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4877 276
Size: 53 83 186 936 3343 2468
Fragments arranged by size:
3343 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI
RsrII SacII SanDI SfiI SfoI SrfI SwaI