Restriction map for
pFN21ASDA1778
(Circular) (Six-base) MAPSORT of: pFN21ASDA1778.seq Check: 9970 from: 1 to: 5704
pFN21ASDA1778 5704 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2927 2927
Size: 5704
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2750 3514 278
Size: 53 83 186 936 1214 764 2468
Fragments arranged by size:
2468 1214 936 764 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AfeI AGC'GCT
Cuts at: 2816 2816
Size: 5704
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2042 4603 5580 1203
Size: 839 2561 977 1327
Fragments arranged by size:
2561 1327 977 839
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 2872 4433 2872
Size: 1561 4143
AleI CACnn'nnGTG
Cuts at: 1166 2177 1166
Size: 1011 4693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2375 1184
Size: 1191 4513
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 5672
AlwNI CAG_nnn'CTG
Cuts at: 2236 2623 3447 5019 2236
Size: 387 824 1572 2921
Fragments arranged by size:
2921 1572 824 387
ApaI G_GGCC'C
Cuts at: 2600 2600
Size: 5704
ApaLI G'TGCA_C
Cuts at: 2459 3761 4917 2459
Size: 1302 1156 3246
Fragments arranged by size:
3246 1302 1156
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 2345 2408 2431 2641 3029 1870
Size: 73 298 104 63 23 210 388 4545
Fragments arranged by size:
4545 388 298 210 104 73 63 23
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 2413 2528 2542 2915
Size: 325 217 627 398 228 115 14 373
Cuts at: 2915 3025 4480 618
Size: 110 1455 1842
Fragments arranged by size:
1842 1455 627 398 373 325 228 217
115 110 14
BanII G_rGCy'C
Cuts at: 1391 2397 2474 2514 2600 3023 1391
Size: 1006 77 40 86 423 4072
Fragments arranged by size:
4072 1006 423 86 77 40
BbeI G_GCGC'C
Cuts at: 2417 2532 2546 2417
Size: 115 14 5575
Fragments arranged by size:
5575 115 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2977 928
Size: 971 1078 3655
Fragments arranged by size:
3655 1078 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2449 4315 136
Size: 107 122 71 631 605 777 1866 1525
Fragments arranged by size:
1866 1525 777 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2958 5699 2958
Size: 2741 2963
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 2190 2463 2600 2918 3765 4921 1790
Size: 400 273 137 318 847 1156 2573
Fragments arranged by size:
2573 1156 847 400 318 273 137
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2611 3435 4393
Size: 416 53 83 399 24 1194 824 958
Cuts at: 4393 442
Size: 1753
Fragments arranged by size:
1753 1194 958 824 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2571 1056
Size: 1515 4189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2873 4364 1239
Size: 1340 294 1491 2579
Fragments arranged by size:
2579 1491 1340 294
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3829 4694 4992 2822
Size: 1007 865 298 3534
Fragments arranged by size:
3534 1007 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2045 2158 3001 3495 493
Size: 711 841 113 843 494 2702
Fragments arranged by size:
2702 843 841 711 494 113
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2414 2529 2543 2703
Size: 53 83 186 936 881 115 14 160
Cuts at: 2703 2747 3511 3893 4528 275
Size: 44 764 382 635 1451
Fragments arranged by size:
1451 936 881 764 635 382 186 160
115 83 53 44 14
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2654 2684 1178
Size: 30 165 30 1251 30 4198
Fragments arranged by size:
4198 1251 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2077 1186
Size: 891 4813
BseYI C'CCAG_C
Cuts at: 2418 2476 4907 5403 2418
Size: 58 2431 496 2719
Fragments arranged by size:
2719 2431 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 2923 1163
Size: 573 419 768 3944
Fragments arranged by size:
3944 768 573 419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3154 3915 4943 1540
Size: 275 186 802 351 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 351 275 186
BsiHKAI G_wGCw'C
Cuts at: 2397 2463 3765 3850 4921 2397
Size: 66 1302 85 1071 3180
Fragments arranged by size:
3180 1302 1071 85 66
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2397 2463 2474 2514 2600 2918
Size: 399 400 207 66 11 40 86 318
Cuts at: 2918 3023 3765 3850 4921 1391
Size: 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 400 399 318 207 105
86 85 66 40 11
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2927 3059 844
Size: 1472 611 132 3489
Fragments arranged by size:
3489 1472 611 132
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 3591 1394
Size: 429 290 1478 3507
Fragments arranged by size:
3507 1478 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2355 2634 4348 4535 1700
Size: 110 545 279 1714 187 2869
Fragments arranged by size:
2869 1714 545 279 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 2723 1925
Size: 798 4906
BssSI C'ACGA_G
Cuts at: 2324 2396 3764 4776 2324
Size: 72 1368 1012 3252
Fragments arranged by size:
3252 1368 1012 72
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 794
Size: 1532 4172
BstBI TT'CG_AA
Cuts at: 2007 4508 2007
Size: 2501 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2958 3034 3401 3786 3803 5255 5341 5353
Size: 986 76 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 986 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2100 2665 2910 513
Size: 552 957 78 565 245 3307
Fragments arranged by size:
3307 957 565 552 245 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 3220 4014 4034 2086
Size: 1134 794 20 3756
Fragments arranged by size:
3756 1134 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DraIII CAC_nnn'GTG
Cuts at: 2896 2896
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2754 3151 4040
Size: 54 1425 50 275 18 924 397 889
Cuts at: 4040 5669 8
Size: 1629 43
Fragments arranged by size:
1629 1425 924 889 397 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2291 3634 1353
Size: 767 171 1343 3423
Fragments arranged by size:
3423 1343 767 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 2714 4291 4665 4811 387
Size: 1064 138 459 666 1577 374 146 1280
Fragments arranged by size:
1577 1280 1064 666 459 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2395
Size: 5704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 2873 3767 4364 5151 5419 1239
Size: 393 947 294 894 597 787 268 1524
Fragments arranged by size:
1524 947 894 787 597 393 294 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 2855
Size: 5704
EcoO109I rG'GnC_Cy
Cuts at: 2794 2794
Size: 5704
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2170 2417 2532 2546 2641 2818 4851 1678
Size: 492 247 115 14 95 177 2033 2531
Fragments arranged by size:
2531 2033 492 247 177 115 95 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2890 4345 4377 4419
Size: 32 135 32 1286 32 1455 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 1455 1286 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2413 2528 2542 2413
Size: 115 14 5575
Fragments arranged by size:
5575 115 14
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 2958
Fragments arranged by size:
2958 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3662 4021 4180 518
Size: 648 1011 1485 359 159 2042
Fragments arranged by size:
2042 1485 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2602 2667 3120 3797 4945 5190 1606
Size: 496 500 65 453 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 500 496 453 245 65
NaeI GCC'GGC
Cuts at: 2357 2636 2357
Size: 279 5425
NarI GG'CG_CC
Cuts at: 2414 2529 2543 2414
Size: 115 14 5575
Fragments arranged by size:
5575 115 14
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4195
Fragments arranged by size:
4195 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2355 2634 2355
Size: 279 5425
NheI G'CTAG_C
Cuts at: 1052 2567 1052
Size: 1515 4189
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2212 3062 4607 1132
Size: 97 983 850 1545 2229
Fragments arranged by size:
2229 1545 983 850 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 2896 1108
Size: 1515 273 3916
Fragments arranged by size:
3916 1515 273
PfoI T'CCnGG_A
Cuts at: 2298 2850 2298
Size: 552 5152
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PmlI CAC'GTG
Cuts at: 2045 2045
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 3773 3805 5286 5318 2011
Size: 32 1730 32 1481 32 2397
Fragments arranged by size:
2397 1730 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 2794
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2596 2596
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2364 2993 3056 830
Size: 1534 629 63 3478
Fragments arranged by size:
3478 1534 629 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
SacI G_AGCT'C
Cuts at: 2397 2397
Size: 5704
SacII CC_GC'GG
Cuts at: 2103 2668 2103
Size: 565 5139
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SanDI GG'GwC_CC
Cuts at: 2794 2794
Size: 5704
SapI GCTCTTCn'nnn_
Cuts at: 2120 2120
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 2378 3952 5664 2378
Size: 1574 1712 2418
Fragments arranged by size:
2418 1712 1574
SfcI C'TryA_G
Cuts at: 826 1045 2360 2989 3052 4187 4868 5059 826
Size: 219 1315 629 63 1135 681 191 1471
Fragments arranged by size:
1471 1315 1135 681 629 219 191 63
SfoI GGC'GCC
Cuts at: 2415 2530 2544 2415
Size: 115 14 5575
Fragments arranged by size:
5575 115 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 2243 2433 3031 1872
Size: 371 190 598 4545
Fragments arranged by size:
4545 598 371 190
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 3808 4709 4971 820
Size: 197 926 894 971 901 262 1553
Fragments arranged by size:
1553 971 926 901 894 262 197
SnaBI TAC'GTA
Cuts at: 493 3001 3495 493
Size: 2508 494 2702
Fragments arranged by size:
2702 2508 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2861 1517
Size: 1344 4360
StyI C'CwwG_G
Cuts at: 513 1065 2022 2757 3143 513
Size: 552 957 735 386 3074
Fragments arranged by size:
3074 957 735 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 3731 3748 3901 4086 4531 325
Size: 1255 888 1263 17 153 185 445 1498
Fragments arranged by size:
1498 1263 1255 888 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 3950 5662
Size: 274 80 33 51 1443 399 1574 1712
Cuts at: 5662 96
Size: 138
Fragments arranged by size:
1712 1574 1443 399 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 5664 5696 2100
Size: 32 3532 32 2108
Fragments arranged by size:
3532 2108 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 2241 2431 3029 1870
Size: 371 190 598 4545
Fragments arranged by size:
4545 598 371 190
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2748 3512 276
Size: 53 83 186 936 1214 764 2468
Fragments arranged by size:
2468 1214 936 764 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI
PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI