Restriction map for
pFN21ASDA1769
(Circular) (Six-base) MAPSORT of: pFN21ASDA1769.seq Check: 1021 from: 1 to: 10969
pFN21ASDA1769 10969 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2954 5073 5296 5887 5956 6898 7343 2954
Size: 2119 223 591 69 942 445 6580
Fragments arranged by size:
6580 2119 942 591 445 223 69
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4560 8779 278
Size: 53 83 186 936 3024 4219 2468
Fragments arranged by size:
4219 3024 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5712 6434 8312 1937
Size: 3775 722 1878 4594
Fragments arranged by size:
4594 3775 1878 722
Acc65I G'GTAC_C
Cuts at: 3699 5346 6004 6117 7554 8290 3699
Size: 1647 658 113 1437 736 6378
Fragments arranged by size:
6378 1647 1437 736 658 113
AclI AA'CG_TT
Cuts at: 1563 9096 9469 1563
Size: 7533 373 3063
Fragments arranged by size:
7533 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 4028 4655 5428 6025 6197 6307 7052
Size: 2288 108 627 773 597 172 110 745
Cuts at: 7052 7667 9032 10416 10684 1632
Size: 615 1365 1384 268 1917
Fragments arranged by size:
2288 1917 1384 1365 773 745 627 615
597 268 172 110 108
AfeI AGC'GCT
Cuts at: 5246 5831 5246
Size: 585 10384
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10772
AflIII A'CryG_T
Cuts at: 1203 2691 3942 5319 9868 10845 1203
Size: 1488 1251 1377 4549 977 1327
Fragments arranged by size:
4549 1488 1377 1327 1251 977
AgeI A'CCGG_T
Cuts at: 6114 7536 9800 6114
Size: 1422 2264 7283
Fragments arranged by size:
7283 2264 1422
AhdI GACnn_n'nnGTC
Cuts at: 9698 9698
Size: 10969
AleI CACnn'nnGTG
Cuts at: 1166 4268 4436 4722 6222 1166
Size: 3102 168 286 1500 5913
Fragments arranged by size:
5913 3102 1500 286 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4512 4740 5478 6002 7121 7178 1184
Size: 3328 228 738 524 1119 57 4975
Fragments arranged by size:
4975 3328 1119 738 524 228 57
AlwNI CAG_nnn'CTG
Cuts at: 2332 2778 2851 2956 3138 3491 4191 4548 5377
Size: 446 73 105 182 353 700 357 829
Cuts at: 5377 5527 5809 6048 6220 6330 7352 7663 8712
Size: 150 282 239 172 110 1022 311 1049
Cuts at: 8712 10284 2332
Size: 1572 3017
Fragments arranged by size:
3017 1572 1049 1022 829 700 446 357
353 311 282 239 182 172 150 110
105 73
ApaI G_GGCC'C
Cuts at: 2443 3776 6106 6393 6839 8119 2443
Size: 1333 2330 287 446 1280 5293
Fragments arranged by size:
5293 2330 1333 1280 446 287
ApaLI G'TGCA_C
Cuts at: 2835 2939 4424 4948 6512 9026 10182 2835
Size: 104 1485 524 1564 2514 1156 3622
Fragments arranged by size:
3622 2514 1564 1485 1156 524 104
ApoI r'AATT_y
Cuts at: 1493 8278 8504 8656 10765 1493
Size: 6785 226 152 2109 1697
Fragments arranged by size:
6785 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9523 160
Size: 872 8491 1606
Fragments arranged by size:
8491 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10969
AvaI C'yCGr_G
Cuts at: 1870 1943 2102 2442 3143 3204 3422 3617 3692
Size: 73 159 340 701 61 218 195 75
Cuts at: 3692 3769 3863 4720 5268 5937 6106 6296 6350
Size: 77 94 857 548 669 169 190 54
Cuts at: 6350 6386 7122 8294 1870
Size: 36 736 1172 4545
Fragments arranged by size:
4545 1172 857 736 701 669 548 340
218 195 190 169 159 94 77 75
73 61 54 36
AvrII C'CTAG_G
Cuts at: 2866 2866
Size: 10969
BamHI G'GATC_C
Cuts at: 5726 7173 8299 8666 5726
Size: 1447 1126 367 8029
Fragments arranged by size:
8029 1447 1126 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2281 2375 2822 3418
Size: 325 217 627 448 46 94 447 596
Cuts at: 3418 3699 3859 4237 4361 4724 4809 4856 5035
Size: 281 160 378 124 363 85 47 179
Cuts at: 5035 5346 5703 5978 6004 6031 6117 6269 6572
Size: 311 357 275 26 27 86 152 303
Cuts at: 6572 7554 8290 9745 618
Size: 982 736 1455 1842
Fragments arranged by size:
1842 1455 982 736 627 596 448 447
378 363 357 325 311 303 281 275
217 179 160 152 124 94 86 85
47 46 27 26
BanII G_rGCy'C
Cuts at: 1391 2030 2277 2443 3335 3519 3693 3776 5402
Size: 639 247 166 892 184 174 83 1626
Cuts at: 5402 5433 5567 6014 6106 6393 6839 7121 7245
Size: 31 134 447 92 287 446 282 124
Cuts at: 7245 7860 8119 8130 8288 1391
Size: 615 259 11 158 4072
Fragments arranged by size:
4072 1626 892 639 615 447 446 287
282 259 247 184 174 166 158 134
124 92 83 31 11
BbeI G_GCGC'C
Cuts at: 3863 4365 5707 6273 3863
Size: 502 1342 566 8559
Fragments arranged by size:
8559 1342 566 502
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 3417 6860 7655 928
Size: 971 622 896 3443 795 4242
Fragments arranged by size:
4242 3443 971 896 795 622
BbvCI CC'TCA_GC
Cuts at: 2852 3477 4562 6015 6563 7062 7809 2852
Size: 625 1085 1453 548 499 747 6012
Fragments arranged by size:
6012 1453 1085 747 625 548 499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2365 2399 2791 2825 4305 4339 6331
Size: 34 428 34 392 34 1480 34 1992
Cuts at: 6331 6365 9160 9194 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 1992 1480 428 392 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8861 10071 8861
Size: 1210 9759
BclI T'GATC_A
Cuts at: 1548 4707 1548
Size: 3159 7810
BfrBI ATG'CAT
Cuts at: 7597 7597
Size: 10969
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2447 2908 3147
Size: 107 122 71 631 605 775 461 239
Cuts at: 3147 3434 3868 5397 5682 5916 6256 7695 7943
Size: 287 434 1529 285 234 340 1439 248
Cuts at: 7943 9580 136
Size: 1637 1525
Fragments arranged by size:
1637 1529 1525 1439 775 631 605 461
434 340 287 285 248 239 234 122
107 71
BglII A'GATC_T
Cuts at: 3990 10964 3990
Size: 6974 3995
BlpI GC'TnA_GC
Cuts at: 2144 2930 2960 8202 8386 2144
Size: 786 30 5242 184 4727
Fragments arranged by size:
5242 4727 786 184 30
Bme1580I G_kGCm'C
Cuts at: 1790 2443 2827 2839 2943 2974 3423 3776 4242
Size: 653 384 12 104 31 449 353 466
Cuts at: 4242 4428 4455 4530 4952 5040 6106 6393 6516
Size: 186 27 75 422 88 1066 287 123
Cuts at: 6516 6575 6839 8119 9030 10186 1790
Size: 59 264 1280 911 1156 2573
Fragments arranged by size:
2573 1280 1156 1066 911 653 466 449
422 384 353 287 264 186 123 104
88 75 59 31 27 12
BmgBI CAC'GTC
Cuts at: 1318 2086 3415 4474 5356 5554 6127 7375 7653
Size: 768 1329 1059 882 198 573 1248 278
Cuts at: 7653 1318
Size: 4634
Fragments arranged by size:
4634 1329 1248 1059 882 768 573 278
198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2153 3025 3244
Size: 416 53 83 399 24 736 872 219
Cuts at: 3244 6475 6689 7073 7671 8700 9658 442
Size: 3231 214 384 598 1029 958 1753
Fragments arranged by size:
3231 1753 1029 958 872 736 598 416
399 384 219 214 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10969
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5581 6217 7259 7264 8165 5581
Size: 636 1042 5 901 8385
Fragments arranged by size:
8385 1042 901 636 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 4241 5261 5435 5831 5948 6074 6188
Size: 1115 1887 1020 174 396 117 126 114
Cuts at: 6188 6302 6752 6830 7234 7817 9629 1239
Size: 114 450 78 404 583 1812 2579
Fragments arranged by size:
2579 1887 1812 1115 1020 583 450 404
396 174 126 117 114 114 78
Bpu10I CC'TnA_GC
Cuts at: 2276 2852 3477 4562 6015 6563 7062 7109 7809
Size: 576 625 1085 1453 548 499 47 700
Cuts at: 7809 2276
Size: 5436
Fragments arranged by size:
5436 1453 1085 700 625 576 548 499
47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4091 4252 4988 5498 8042 9094 9959 10257 4091
Size: 161 736 510 2544 1052 865 298 4803
Fragments arranged by size:
4803 2544 1052 865 736 510 298 161
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3719 4389 7470 7545 8136 9632 882
Size: 617 2220 670 3081 75 591 1496 2219
Fragments arranged by size:
3081 2220 2219 1496 670 617 591 75
BsaAI yAC'GTr
Cuts at: 493 1204 2590 2668 3945 5322 6061 6432 7729
Size: 711 1386 78 1277 1377 739 371 1297
Cuts at: 7729 8266 8760 493
Size: 537 494 2702
Fragments arranged by size:
2702 1386 1377 1297 1277 739 711 537
494 371 78
BsaBI GATnn'nnATC
Cuts at: 10963 10963
Size: 10969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2556 2702 2997 3057
Size: 53 83 186 936 1023 146 295 60
Cuts at: 3057 3531 3740 3860 4362 4557 4764 5704 5873
Size: 474 209 120 502 195 207 940 169
Cuts at: 5873 6239 6248 6270 6653 8776 9158 9793 275
Size: 366 9 22 383 2123 382 635 1451
Fragments arranged by size:
2123 1451 1023 940 936 635 502 474
383 382 366 295 209 207 195 186
169 146 120 83 60 53 22 9
BsaWI w'CCGG_w
Cuts at: 4041 6114 6550 7170 7536 9402 9800 10074 10221
Size: 2073 436 620 366 1866 398 274 147
Cuts at: 10221 10632 4041
Size: 411 4378
Fragments arranged by size:
4378 2073 1866 620 436 411 398 366
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3387 3417 3749 3779 5899
Size: 30 165 30 1984 30 332 30 2120
Cuts at: 5899 5929 1178
Size: 30 6218
Fragments arranged by size:
6218 2120 1984 332 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 2103 2869 3287 3757 4706 4715 6314
Size: 834 83 766 418 470 949 9 1599
Cuts at: 6314 6662 7681 7814 1186
Size: 348 1019 133 4341
Fragments arranged by size:
4341 1599 1019 949 834 766 470 418
348 133 83 9
BseYI C'CCAG_C
Cuts at: 2771 2778 3036 3218 3334 3889 4153 5386 5520
Size: 7 258 182 116 555 264 1233 134
Cuts at: 5520 5536 5591 5877 5944 6392 6719 6779 6946
Size: 16 55 286 67 448 327 60 167
Cuts at: 6946 10172 10668 2771
Size: 3226 496 3072
Fragments arranged by size:
3226 3072 1233 555 496 448 327 286
264 258 182 167 134 116 67 60
55 16 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 2533 2768 3857 5033 5051 5087
Size: 573 714 83 235 1089 1176 18 36
Cuts at: 5087 5480 5528 5915 5921 5990 6932 7148 7315
Size: 393 48 387 6 69 942 216 167
Cuts at: 7315 7365 7460 7751 1163
Size: 50 95 291 4381
Fragments arranged by size:
4381 1176 1089 942 714 573 393 387
291 235 216 167 95 83 69 50
48 36 18 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2141 2194 2289 2451 2788 4312
Size: 275 186 140 53 95 162 337 1524
Cuts at: 4312 4732 5260 5681 6861 8419 9180 10208 1540
Size: 420 528 421 1180 1558 761 1028 2301
Fragments arranged by size:
2301 1558 1524 1180 1028 761 528 421
420 337 275 186 162 140 95 53
BsiHKAI G_wGCw'C
Cuts at: 2030 2277 2457 2839 2931 2943 3009 3519 4428
Size: 247 180 382 92 12 66 510 909
Cuts at: 4428 4952 5658 6060 6516 7245 8003 9030 9115
Size: 524 706 402 456 729 758 1027 85
Cuts at: 9115 10186 2030
Size: 1071 2813
Fragments arranged by size:
2813 1071 1027 909 758 729 706 524
510 456 402 382 247 180 92 85
66 12
BsmI GAATG_Cn'
Cuts at: 8480 8573 8480
Size: 93 10876
BsmBI CGTCTCn'nnnn_
Cuts at: 3181 3390 5597 5780 3181
Size: 209 2207 183 8370
Fragments arranged by size:
8370 2207 209 183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2277 2443 2457 2827 2839 2931
Size: 399 240 247 166 14 370 12 92
Cuts at: 2931 2943 2974 3009 3335 3423 3519 3693 3776
Size: 12 31 35 326 88 96 174 83
Cuts at: 3776 4242 4428 4455 4530 4952 5040 5402 5433
Size: 466 186 27 75 422 88 362 31
Cuts at: 5433 5567 5658 6014 6060 6106 6393 6516 6575
Size: 134 91 356 46 46 287 123 59
Cuts at: 6575 6839 7121 7245 7860 8003 8119 8130 8288
Size: 264 282 124 615 143 116 11 158
Cuts at: 8288 9030 9115 10186 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 615 466 422 399 370
362 356 326 287 282 264 247 240
186 174 166 158 143 134 124 123
116 96 92 91 88 88 85 83
75 59 46 46 35 31 31 27
14 12 12 11
BspEI T'CCGG_A
Cuts at: 4041 6550 10632 4041
Size: 2509 4082 4378
Fragments arranged by size:
4378 4082 2509
BspHI T'CATG_A
Cuts at: 8858 10588 8858
Size: 1730 9239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2398 2722 2954 3154 3660 4012 5073 5119
Size: 1554 324 232 200 506 352 1061 46
Cuts at: 5119 5296 5334 5887 5956 6714 6898 7343 8324
Size: 177 38 553 69 758 184 445 981
Cuts at: 8324 844
Size: 3489
Fragments arranged by size:
3489 1554 1061 981 758 553 506 445
352 324 232 200 184 177 69 46
38
BsrBI CCG'CTC
Cuts at: 1394 1823 2653 3127 3443 3697 3736 6067 6736
Size: 429 830 474 316 254 39 2331 669
Cuts at: 6736 7513 7552 7744 8856 1394
Size: 777 39 192 1112 3507
Fragments arranged by size:
3507 2331 1112 830 777 669 474 429
316 254 192 39 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 9464 9638 65
Size: 1183 8216 174 1396
Fragments arranged by size:
8216 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2284 4050 4101 4307 4537 4727
Size: 110 326 148 1766 51 206 230 190
Cuts at: 4727 4741 4806 5676 6114 6179 6489 6684 7311
Size: 14 65 870 438 65 310 195 627
Cuts at: 7311 7536 8222 9613 9800 1700
Size: 225 686 1391 187 2869
Fragments arranged by size:
2869 1766 1391 870 686 627 438 326
310 230 225 206 195 190 187 148
110 65 65 51 14
BsrGI T'GTAC_A
Cuts at: 96 2127 7047 96
Size: 2031 4920 4018
Fragments arranged by size:
4920 4018 2031
BssHII G'CGCG_C
Cuts at: 1925 3682 5486 1925
Size: 1757 1804 7408
Fragments arranged by size:
7408 1804 1757
BssSI C'ACGA_G
Cuts at: 2384 2501 2534 3914 4919 7559 7739 8213 9029
Size: 117 33 1380 1005 2640 180 474 816
Cuts at: 9029 10041 2384
Size: 1012 3312
Fragments arranged by size:
3312 2640 1380 1012 1005 816 474 180
117 33
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2461 4816 4947 5377 7352 794
Size: 1667 2355 131 430 1975 4411
Fragments arranged by size:
4411 2355 1975 1667 430 131
BstBI TT'CG_AA
Cuts at: 2007 9773 2007
Size: 7766 3203
BstEII G'GTnAC_C
Cuts at: 1184 2248 3574 5179 6664 1184
Size: 1064 1326 1605 1485 5489
Fragments arranged by size:
5489 1605 1485 1326 1064
BstXI CCAn_nnnn'nTGG
Cuts at: 3754 7684 3754
Size: 3930 7039
BstYI r'GATC_y
Cuts at: 1972 2895 3990 5373 5726 7173 7383 8299 8666
Size: 923 1095 1383 353 1447 210 916 367
Cuts at: 8666 9051 9068 10520 10606 10618 10964 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1447 1383 1095 923 916 385
367 353 346 210 86 17 12
BstZ17I GTA'TAC
Cuts at: 6435 6435
Size: 10969
Bsu36I CC'TnA_GG
Cuts at: 4179 7148 7664 4179
Size: 2969 516 7484
Fragments arranged by size:
7484 2969 516
BtgI C'CryG_G
Cuts at: 513 1065 2697 2818 3060 3156 3297 3468 3503
Size: 552 1632 121 242 96 141 171 35
Cuts at: 3503 4611 4659 4758 5184 5392 5781 6369 6448
Size: 1108 48 99 426 208 389 588 79
Cuts at: 6448 6931 7410 7761 513
Size: 483 479 351 3721
Fragments arranged by size:
3721 1632 1108 588 552 483 479 426
389 351 242 208 171 141 121 99
96 79 48 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4002 6658 7852 7918 487
Size: 683 56 2776 2656 1194 66 3538
Fragments arranged by size:
3538 2776 2656 1194 683 66 56
BtsI GCAGTG_nn'
Cuts at: 2050 3408 3706 5045 5744 5956 8485 9279 9299
Size: 1358 298 1339 699 212 2529 794 20
Cuts at: 9299 2050
Size: 3720
Fragments arranged by size:
3720 2529 1358 1339 794 699 298 212
20
ClaI AT'CG_AT
Cuts at: 1554 2313 5424 1554
Size: 759 3111 7099
Fragments arranged by size:
7099 3111 759
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10934
DraI TTT'AAA
Cuts at: 8273 8273
Size: 10969
DraIII CAC_nnn'GTG
Cuts at: 2242 2506 2242
Size: 264 10705
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2561 4769 7871 9976 809
Size: 507 1245 2208 3102 2105 1802
Fragments arranged by size:
3102 2208 2105 1802 1245 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2138 2433
Size: 54 1425 50 275 18 265 43 295
Cuts at: 2433 2448 2592 2785 3267 3294 3500 3999 4297
Size: 15 144 193 482 27 206 499 298
Cuts at: 4297 4309 4440 4539 4576 4590 4627 4729 4776
Size: 12 131 99 37 14 37 102 47
Cuts at: 4776 5056 5257 5290 5502 5649 5678 5778 5834
Size: 280 201 33 212 147 29 100 56
Cuts at: 5834 5917 6174 6372 7407 7723 7944 8224 8416
Size: 83 257 198 1035 316 221 280 192
Cuts at: 8416 9305 10934 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1035 889 499 482 316 298
295 280 280 275 265 257 221 212
206 201 198 193 192 147 144 131
102 100 99 83 56 54 50 47
43 43 37 37 33 29 27 18
15 14 12
EagI C'GGCC_G
Cuts at: 1537 1812 2138 2448 2785 4309 4729 5257 5678
Size: 275 326 310 337 1524 420 528 421
Cuts at: 5678 8416 1537
Size: 2738 4090
Fragments arranged by size:
4090 2738 1524 528 421 420 337 326
310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2480 3896 3902 4112 4631 5591 6524 6549
Size: 1127 1416 6 210 519 960 933 25
Cuts at: 6549 7109 7250 8899 1353
Size: 560 141 1649 3423
Fragments arranged by size:
3423 1649 1416 1127 960 933 560 519
210 141 25 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2195 2619 4712 6266 9556 9930
Size: 1064 138 606 424 2093 1554 3290 374
Cuts at: 9930 10076 387
Size: 146 1280
Fragments arranged by size:
3290 2093 1554 1280 1064 606 424 374
146 138
EcoICRI GAG'CTC
Cuts at: 2028 2275 3517 7243 2028
Size: 247 1242 3726 5754
Fragments arranged by size:
5754 3726 1242 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 3920 4028 4241 4655 5261 5428
Size: 393 722 1566 108 213 414 606 167
Cuts at: 5428 5435 5831 5948 6025 6074 6188 6197 6302
Size: 7 396 117 77 49 114 9 105
Cuts at: 6302 6307 6752 6830 7052 7234 7667 7817 9032
Size: 5 445 78 222 182 433 150 1215
Cuts at: 9032 9629 10416 10684 1239
Size: 597 787 268 1524
Fragments arranged by size:
1566 1524 1215 787 722 606 597 445
433 414 396 393 268 222 213 182
167 150 117 114 108 105 78 77
49 9 7 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 3204 3269 3629 4043 4550 5231 7799 2870
Size: 334 65 360 414 507 681 2568 6040
Fragments arranged by size:
6040 2568 681 507 414 360 334 65
EcoO109I rG'GnC_Cy
Cuts at: 2344 2439 2863 3081 3148 3262 3426 3773 3949
Size: 95 424 218 67 114 164 347 176
Cuts at: 3949 4012 4024 4201 4403 5008 5236 6103 6835
Size: 63 12 177 202 605 228 867 732
Cuts at: 6835 6923 6953 7183 7258 7660 7915 2344
Size: 88 30 230 75 402 255 5398
Fragments arranged by size:
5398 867 732 605 424 402 347 255
230 228 218 202 177 176 164 114
95 88 75 67 63 30 12
EcoRI G'AATT_C
Cuts at: 8278 8656 8278
Size: 378 10591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5557 7297 5557
Size: 1740 9229
FseI GG_CCGG'CC
Cuts at: 5680 6688 5680
Size: 1008 9961
FspI TGC'GCA
Cuts at: 2730 9475 2730
Size: 6745 4224
HaeII r_GCGC'y
Cuts at: 1678 3196 3863 4365 4497 5248 5387 5707 5833
Size: 1518 667 502 132 751 139 320 126
Cuts at: 5833 6273 6481 7987 10116 1678
Size: 440 208 1506 2129 2531
Fragments arranged by size:
2531 2129 1518 1506 751 667 502 440
320 208 139 132 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3137 3169 3725 3749 3752
Size: 32 135 32 1565 32 556 24 3
Cuts at: 3752 3757 3781 3784 6068 6100 6638 6670 7917
Size: 5 24 3 2284 32 538 32 1247
Cuts at: 7917 7949 9610 9642 9684 9716 1373
Size: 32 1661 32 42 32 2626
Fragments arranged by size:
2626 2284 1661 1565 1247 556 538 135
42 32 32 32 32 32 32 32
32 24 24 5 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 6421 7393 8313 8559 669
Size: 988 281 4483 972 920 246 3079
Fragments arranged by size:
4483 3079 988 972 920 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10969
HpaI GTT'AAC
Cuts at: 8559 8559
Size: 10969
KasI G'GCGC_C
Cuts at: 3859 4361 5703 6269 3859
Size: 502 1342 566 8559
Fragments arranged by size:
8559 1342 566 502
KpnI G_GTAC'C
Cuts at: 3703 5350 6008 6121 7558 8294 3703
Size: 1647 658 113 1437 736 6378
Fragments arranged by size:
6378 1647 1437 736 658 113
MfeI C'AATT_G
Cuts at: 8568 8568
Size: 10969
MluI A'CGCG_T
Cuts at: 10845 10845
Size: 10969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4227 4848 7005 7182 10083 10267 1305
Size: 2922 621 2157 177 2901 184 2007
Fragments arranged by size:
2922 2901 2157 2007 621 184 177
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 2594 4442 4592 4629
Size: 54 1425 343 603 159 1848 150 37
Cuts at: 4629 5919 7409 7725 10
Size: 1290 1490 316 3254
Fragments arranged by size:
3254 1848 1490 1425 1290 603 343 316
159 150 54 37
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 4268 4436 4722 4886 6222 6536
Size: 648 1530 1572 168 286 164 1336 314
Cuts at: 6536 8927 9286 9445 518
Size: 2391 359 159 2042
Fragments arranged by size:
2391 2042 1572 1530 1336 648 359 314
286 168 164 159
MspA1I CmG'CkG
Cuts at: 1606 2091 2143 2356 2547 2775 3040 3227 3455
Size: 485 52 213 191 228 265 187 228
Cuts at: 3455 3754 4153 4633 4681 5262 5368 5479 5571
Size: 299 399 480 48 581 106 111 92
Cuts at: 5571 5647 5806 5881 5905 6262 6327 6661 7489
Size: 76 159 75 24 357 65 334 828
Cuts at: 7489 7834 8038 8385 9062 10210 10455 1606
Size: 345 204 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 581 485 480 399
357 347 345 334 299 265 245 228
228 213 204 191 187 159 111 106
92 76 75 65 52 48 24
NaeI GCC'GGC
Cuts at: 4052 4103 4309 4539 4729 4743 4808 5678 6686
Size: 51 206 230 190 14 65 870 1008
Cuts at: 6686 8224 4052
Size: 1538 6797
Fragments arranged by size:
6797 1538 1008 870 230 206 190 65
51 14
NarI GG'CG_CC
Cuts at: 3860 4362 5704 6270 3860
Size: 502 1342 566 8559
Fragments arranged by size:
8559 1342 566 502
NcoI C'CATG_G
Cuts at: 513 1065 2697 3156 3297 4611 5392 513
Size: 552 1632 459 141 1314 781 6090
Fragments arranged by size:
6090 1632 1314 781 552 459 141
NdeI CA'TA_TG
Cuts at: 387 7572 387
Size: 7185 3784
NgoMIV G'CCGG_C
Cuts at: 4050 4101 4307 4537 4727 4741 4806 5676 6684
Size: 51 206 230 190 14 65 870 1008
Cuts at: 6684 8222 4050
Size: 1538 6797
Fragments arranged by size:
6797 1538 1008 870 230 206 190 65
51 14
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10969
NotI GC'GGCC_GC
Cuts at: 8416 8416
Size: 10969
NsiI A_TGCA'T
Cuts at: 7599 7599
Size: 10969
NspI r_CATG'y
Cuts at: 1132 1229 2407 2695 5197 7597 8327 9872 1132
Size: 97 1178 288 2502 2400 730 1545 2229
Fragments arranged by size:
2502 2400 2229 1545 1178 730 288 97
PasI CC'CwG_GG
Cuts at: 1348 2966 4357 4455 4861 7840 1348
Size: 1618 1391 98 406 2979 4477
Fragments arranged by size:
4477 2979 1618 1391 406 98
PciI A'CATG_T
Cuts at: 2691 9868 2691
Size: 7177 3792
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 2778 3043 4191 4885 5527 5668 5750
Size: 1224 446 265 1148 694 642 141 82
Cuts at: 5750 5926 6532 6790 8052 1108
Size: 176 606 258 1262 4025
Fragments arranged by size:
4025 1262 1224 1148 694 642 606 446
265 258 176 141 82
PfoI T'CCnGG_A
Cuts at: 2064 4164 5856 5925 6729 7368 8184 2064
Size: 2100 1692 69 804 639 816 4849
Fragments arranged by size:
4849 2100 1692 816 804 639 69
PmeI GTTT'AAAC
Cuts at: 8273 8273
Size: 10969
PmlI CAC'GTG
Cuts at: 2668 3945 5322 6061 7729 2668
Size: 1277 1377 739 1668 5908
Fragments arranged by size:
5908 1668 1377 1277 739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9038 9070 10551 10583 9038
Size: 32 1481 32 9424
Fragments arranged by size:
9424 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2863 3262 3949 4012 4024 4403 6953 7258 2863
Size: 399 687 63 12 379 2550 305 6574
Fragments arranged by size:
6574 2550 687 399 379 305 63 12
PshAI GACnn'nnGTC
Cuts at: 1613 4401 1613
Size: 2788 8181
PsiI TTA'TAA
Cuts at: 8539 8539
Size: 10969
PspOMI G'GGCC_C
Cuts at: 2439 3772 6102 6389 6835 8115 2439
Size: 1333 2330 287 446 1280 5293
Fragments arranged by size:
5293 2330 1333 1280 446 287
PspXI vC'TCGA_Gb
Cuts at: 6296 6350 6296
Size: 54 10915
PstI C_TGCA'G
Cuts at: 830 2172 3454 3630 4059 4278 5218 5307 5646
Size: 1342 1282 176 429 219 940 89 339
Cuts at: 5646 5829 5967 5991 6267 6710 7233 8243 8321
Size: 183 138 24 276 443 523 1010 78
Cuts at: 8321 830
Size: 3478
Fragments arranged by size:
3478 1342 1282 1010 940 523 443 429
339 276 219 183 176 138 89 78
24
PvuI CG_AT'CG
Cuts at: 2001 6861 2001
Size: 4860 6109
PvuII CAG'CTG
Cuts at: 2775 3040 4633 4681 5647 5806 6262 6327 7489
Size: 265 1593 48 966 159 456 65 1162
Cuts at: 7489 7834 2775
Size: 345 5910
Fragments arranged by size:
5910 1593 1162 966 456 345 265 159
65 48
RsrII CG'GwC_CG
Cuts at: 2134 4821 2134
Size: 2687 8282
SacI G_AGCT'C
Cuts at: 2030 2277 3519 7245 2030
Size: 247 1242 3726 5754
Fragments arranged by size:
5754 3726 1242 247
SalI G'TCGA_C
Cuts at: 1936 8311 1936
Size: 6375 4594
SanDI GG'GwC_CC
Cuts at: 3949 4403 6953 3949
Size: 454 2550 7965
Fragments arranged by size:
7965 2550 454
SapI GCTCTTCn'nnn_
Cuts at: 4112 4631 5591 7109 7250 4112
Size: 519 960 1518 141 7831
Fragments arranged by size:
7831 1518 960 519 141
SbfI CC_TGCA'GG
Cuts at: 5218 5967 8321 5218
Size: 749 2354 7866
Fragments arranged by size:
7866 2354 749
ScaI AGT'ACT
Cuts at: 2174 9217 10929 2174
Size: 7043 1712 2214
Fragments arranged by size:
7043 2214 1712
SexAI A'CCwGG_T
Cuts at: 6841 7260 6841
Size: 419 10550
SfcI C'TryA_G
Cuts at: 826 1045 2168 3450 3626 4033 4055 4274 5214
Size: 219 1123 1282 176 407 22 219 940
Cuts at: 5214 5303 5642 5825 5963 5987 6263 6706 7229
Size: 89 339 183 138 24 276 443 523
Cuts at: 7229 8239 8317 9452 10133 10324 826
Size: 1010 78 1135 681 191 1471
Fragments arranged by size:
1471 1282 1135 1123 1010 940 681 523
443 407 339 276 219 219 191 183
176 138 89 78 24 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 2447 5682 2447
Size: 3235 7734
SfoI GGC'GCC
Cuts at: 3861 4363 5705 6271 3861
Size: 502 1342 566 8559
Fragments arranged by size:
8559 1342 566 502
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10969
SgrAI Cr'CCGG_yG
Cuts at: 1700 6179 1700
Size: 4479 6490
SmaI CCC'GGG
Cuts at: 1872 2444 3424 4722 5270 5939 6388 7124 8296
Size: 572 980 1298 548 669 449 736 1172
Cuts at: 8296 1872
Size: 4545
Fragments arranged by size:
4545 1298 1172 980 736 669 572 548
449
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4070 4231 5003 5513 6296 6350
Size: 197 926 2127 161 772 510 783 54
Cuts at: 6350 8057 9073 9974 10236 820
Size: 1707 1016 901 262 1553
Fragments arranged by size:
2127 1707 1553 1016 926 901 783 772
510 262 197 161 54
SnaBI TAC'GTA
Cuts at: 493 8266 8760 493
Size: 7773 494 2702
Fragments arranged by size:
7773 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10969
SphI G_CATG'C
Cuts at: 1132 7597 8327 1132
Size: 6465 730 3774
Fragments arranged by size:
6465 3774 730
SrfI GCCC'GGGC
Cuts at: 2444 2444
Size: 10969
SspI AAT'ATT
Cuts at: 5 52 8893 5
Size: 47 8841 2081
Fragments arranged by size:
8841 2081 47
StuI AGG'CCT
Cuts at: 1517 2756 5441 7214 7898 1517
Size: 1239 2685 1773 684 4588
Fragments arranged by size:
4588 2685 1773 1239 684
StyI C'CwwG_G
Cuts at: 513 1065 2595 2697 2866 3156 3297 4266 4611
Size: 552 1530 102 169 290 141 969 345
Cuts at: 4611 5392 7785 8408 513
Size: 781 2393 623 3074
Fragments arranged by size:
3074 2393 1530 969 781 623 552 345
290 169 141 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 5165 5214 6430 8996 9013 9166
Size: 1255 621 2964 49 1216 2566 17 153
Cuts at: 9166 9351 9796 325
Size: 185 445 1498
Fragments arranged by size:
2964 2566 1498 1255 1216 621 445 185
153 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 2172 4224
Size: 274 80 33 51 1443 150 45 2052
Cuts at: 4224 4896 6966 7047 9215 10927 96
Size: 672 2070 81 2168 1712 138
Fragments arranged by size:
2168 2070 2052 1712 1443 672 274 150
138 81 80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3387 3419 5899 5931 10929 10961 3387
Size: 32 2480 32 4998 32 3395
Fragments arranged by size:
4998 3395 2480 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 3605 5177 6203 7262 1316
Size: 843 1446 1572 1026 1059 5023
Fragments arranged by size:
5023 1572 1446 1059 1026 843
XbaI T'CTAG_A
Cuts at: 8305 8305
Size: 10969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3994 4292 4414 6676 6727 6787 6988 7258 7792
Size: 298 122 2262 51 60 201 270 534
Cuts at: 7792 8026 3994
Size: 234 6937
Fragments arranged by size:
6937 2262 534 298 270 234 201 122
60 51
XhoI C'TCGA_G
Cuts at: 1943 6296 6350 1943
Size: 4353 54 6562
Fragments arranged by size:
6562 4353 54
XmaI C'CCGG_G
Cuts at: 1870 2442 3422 4720 5268 5937 6386 7122 8294
Size: 572 980 1298 548 669 449 736 1172
Cuts at: 8294 1870
Size: 4545
Fragments arranged by size:
4545 1298 1172 980 736 669 572 548
449
XmnI GAAnn'nnTTC
Cuts at: 6818 9098 6818
Size: 2280 8689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4558 8777 276
Size: 53 83 186 936 3024 4219 2468
Fragments arranged by size:
4219 3024 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI EcoRV FspAI NruI PacI
PsrI SacII SwaI