Restriction map for
pFN21ASDA1750
(Circular) (Six-base) MAPSORT of: pFN21ASDA1750.seq Check: 8476 from: 1 to: 5980
pFN21ASDA1750 5980 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 4043 5427 5695 1632
Size: 1040 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 1040 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2381 3723 5295 2381
Size: 1342 1572 3066
Fragments arranged by size:
3066 1572 1342
ApaI G_GGCC'C
Cuts at: 2296 2607 2296
Size: 311 5669
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 3289 3515 3667 5776 1493
Size: 1796 226 152 2109 1697
Fragments arranged by size:
2109 1796 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 2153 2240 2295 2338 2767 3305
Size: 73 148 62 87 55 43 429 538
Cuts at: 3305 1870
Size: 4545
Fragments arranged by size:
4545 538 429 148 87 73 62 55
43
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 4756 618
Size: 325 217 627 1514 1455 1842
Fragments arranged by size:
1842 1514 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2296 2607 2877 3184 3299 1391
Size: 905 311 270 307 115 4072
Fragments arranged by size:
4072 905 311 307 270 115
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2166 2377 928
Size: 971 267 211 4531
Fragments arranged by size:
4531 971 267 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2340 2374 4171 4205 1903
Size: 34 403 34 1797 34 3678
Fragments arranged by size:
3678 1797 403 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2623 3872 5082 2623
Size: 1249 1210 3521
Fragments arranged by size:
3521 1249 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2296 2607 4041 5197 1790
Size: 506 311 1434 1156 2573
Fragments arranged by size:
2573 1434 1156 506 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2810 1056
Size: 1754 4226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2517 2569 2517
Size: 52 5928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2657 2678 2686 3014 4640 1239
Size: 852 566 21 8 328 1626 2579
Fragments arranged by size:
2579 1626 852 566 328 21 8
Bpu10I CC'TnA_GC
Cuts at: 3038 3038
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4970 5268 4105
Size: 865 298 4817
Fragments arranged by size:
4817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3277 3771 493
Size: 711 2073 494 2702
Fragments arranged by size:
2702 2073 711 494
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3787 4169 4804
Size: 53 83 186 936 582 1672 382 635
Cuts at: 4804 275
Size: 1451
Fragments arranged by size:
1672 1451 936 635 582 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3080 3110 1178
Size: 30 165 30 1677 30 4048
Fragments arranged by size:
4048 1677 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2261 1186
Size: 1036 39 4905
Fragments arranged by size:
4905 1036 39
BseYI C'CCAG_C
Cuts at: 2564 2608 2802 5183 5679 2564
Size: 44 194 2381 496 2865
Fragments arranged by size:
2865 2381 496 194 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 2666 1163
Size: 573 465 465 4477
Fragments arranged by size:
4477 573 465 465
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 2920 3430 4191 5219 1540
Size: 275 186 31 888 510 761 1028 2301
Fragments arranged by size:
2301 1028 888 761 510 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 2877 2949 4041 4126 5197 2877
Size: 72 1092 85 1071 3660
Fragments arranged by size:
3660 1092 1071 85 72
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2296 2607 2877 2949 3184 3299 4041
Size: 399 506 311 270 72 235 115 742
Cuts at: 4041 4126 5197 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 506 399 311 270 235
115 85 72
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3867 1394
Size: 429 205 1839 3507
Fragments arranged by size:
3507 1839 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 2442 4624 4811 1700
Size: 110 449 183 2182 187 2869
Fragments arranged by size:
2869 2182 449 187 183 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 3183 4040 5052 3183
Size: 857 1012 4111
Fragments arranged by size:
4111 1012 857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 2007 4784 2007
Size: 2777 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 3009 3009
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 3310 3677 4062 4079 5531 5617 5629 5975
Size: 1338 367 385 17 1452 86 12 346
Cuts at: 5975 1972
Size: 1977
Fragments arranged by size:
1977 1452 1338 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2502 2502
Size: 5980
BtgI C'CryG_G
Cuts at: 513 1065 2311 2322 2406 2487 513
Size: 552 1246 11 84 81 4006
Fragments arranged by size:
4006 1246 552 84 81 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 4290 4310 3496
Size: 794 20 5166
Fragments arranged by size:
5166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2325 2370
Size: 54 1425 50 275 18 199 296 45
Cuts at: 2370 2977 3427 4316 5945 8
Size: 607 450 889 1629 43
Fragments arranged by size:
1629 1425 889 607 450 296 275 199
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2029 3427 1537
Size: 275 217 1398 4090
Fragments arranged by size:
4090 1398 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 2865 3910 1353
Size: 1224 288 1045 3423
Fragments arranged by size:
3423 1224 1045 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2693 4567 4941 5087 387
Size: 1064 138 498 606 1874 374 146 1280
Fragments arranged by size:
1874 1280 1064 606 498 374 146 138
EcoICRI GAG'CTC
Cuts at: 2875 2875
Size: 5980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2657 2672 2678 2686 3014 4043
Size: 393 459 566 15 6 8 328 1029
Cuts at: 4043 4640 5427 5695 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1029 787 597 566 459 393 328
268 15 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 3041
Size: 5980
EcoO109I rG'GnC_Cy
Cuts at: 2467 2498 2603 2604 2685 2467
Size: 31 105 1 81 5762
Fragments arranged by size:
5762 105 81 31 1
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2368 5127 1678
Size: 690 2759 2531
Fragments arranged by size:
2759 2531 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 3017 3049 4621
Size: 32 135 32 1010 32 403 32 1572
Cuts at: 4621 4653 4695 4727 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1572 1010 403 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 3294 5094 5278 1305
Size: 1111 878 1800 184 2007
Fragments arranged by size:
2007 1800 1111 878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2979 10
Size: 54 1425 343 1147 3011
Fragments arranged by size:
3011 1425 1147 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3938 4297 4456 518
Size: 648 2772 359 159 2042
Fragments arranged by size:
2772 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2324 2399 2489 2564 2650 3396 4073
Size: 610 108 75 90 75 86 746 677
Cuts at: 4073 5221 5466 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 610 245 108 90
86 75 75
NaeI GCC'GGC
Cuts at: 2261 2261
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5428
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 2259 2259
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 2806 1052
Size: 1754 4226
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 3338 4883 1132
Size: 97 2109 1545 2229
Fragments arranged by size:
2229 2109 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3017 1348
Size: 1669 4311
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2702 3223 2702
Size: 521 5459
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspOMI G'GGCC_C
Cuts at: 2292 2603 2292
Size: 311 5669
PstI C_TGCA'G
Cuts at: 830 2730 3332 830
Size: 1900 602 3478
Fragments arranged by size:
3478 1900 602
PvuI CG_AT'CG
Cuts at: 2001 2920 2001
Size: 919 5061
PvuII CAG'CTG
Cuts at: 2564 2650 2564
Size: 86 5894
SacI G_AGCT'C
Cuts at: 2877 2877
Size: 5980
SacII CC_GC'GG
Cuts at: 2325 2490 2325
Size: 165 5815
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SapI GCTCTTCn'nnn_
Cuts at: 2865 2865
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2479 2726 3328 4463 5144 5335 826
Size: 219 1434 247 602 1135 681 191 1471
Fragments arranged by size:
1471 1434 1135 681 602 247 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2340 2769 3307 1872
Size: 468 429 538 4545
Fragments arranged by size:
4545 538 468 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4084 4985 5247 820
Size: 197 926 2141 901 262 1553
Fragments arranged by size:
2141 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3277 3771 493
Size: 2784 494 2702
Fragments arranged by size:
2784 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 3419 513
Size: 552 2354 3074
Fragments arranged by size:
3074 2354 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 2952 3066 4007 4024 4177 4362
Size: 1255 600 772 114 941 17 153 185
Cuts at: 4362 4807 325
Size: 445 1498
Fragments arranged by size:
1498 1255 941 772 600 445 185 153
114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2948 2980 5940 5972 2948
Size: 32 2960 32 2956
Fragments arranged by size:
2960 2956 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2381 1316
Size: 1065 4915
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 2338 2767 3305 1870
Size: 468 429 538 4545
Fragments arranged by size:
4545 538 468 429
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI