Restriction map for
pFN21ASDA1736
(Circular) (Six-base) MAPSORT of: pFN21ASDA1736.seq Check: 8194 from: 1 to: 8443
pFN21ASDA1736 8443 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6253 278
Size: 53 83 186 936 4717 2468
Fragments arranged by size:
4717 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2521 5786 1937
Size: 584 3265 4594
Fragments arranged by size:
4594 3265 584
Acc65I G'GTAC_C
Cuts at: 5552 5764 5552
Size: 212 8231
AclI AA'CG_TT
Cuts at: 1563 6570 6943 1563
Size: 5007 373 3063
Fragments arranged by size:
5007 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2188 3118 3399 3503 3545 4762 5096 5153
Size: 556 930 281 104 42 1217 334 57
Cuts at: 5153 6506 7890 8158 1632
Size: 1353 1384 268 1917
Fragments arranged by size:
1917 1384 1353 1217 930 556 334 281
268 104 57 42
AfeI AGC'GCT
Cuts at: 5629 5629
Size: 8443
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8246
AflIII A'CryG_T
Cuts at: 1203 4864 7342 8319 1203
Size: 3661 2478 977 1327
Fragments arranged by size:
3661 2478 1327 977
AgeI A'CCGG_T
Cuts at: 7274 7274
Size: 8443
AhdI GACnn_n'nnGTC
Cuts at: 2900 4253 7172 2900
Size: 1353 2919 4171
Fragments arranged by size:
4171 2919 1353
AleI CACnn'nnGTG
Cuts at: 1166 2237 3681 5135 1166
Size: 1071 1444 1454 4474
Fragments arranged by size:
4474 1454 1444 1071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2832 3884 1184
Size: 1648 1052 5743
Fragments arranged by size:
5743 1648 1052
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2643 2675 2643
Size: 32 8411
AlwNI CAG_nnn'CTG
Cuts at: 2116 2476 2961 3272 3568 3580 4552 5161 5237
Size: 360 485 311 296 12 972 609 76
Cuts at: 5237 5302 6186 7758 2116
Size: 65 884 1572 2801
Fragments arranged by size:
2801 1572 972 884 609 485 360 311
296 76 65 12
ApaLI G'TGCA_C
Cuts at: 6500 7656 6500
Size: 1156 7287
ApoI r'AATT_y
Cuts at: 1493 3999 5752 5978 6130 8239 1493
Size: 2506 1753 226 152 2109 1697
Fragments arranged by size:
2506 2109 1753 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6997 160
Size: 872 5965 1606
Fragments arranged by size:
5965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8443
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2973 3496 3878 5475 5768 1870
Size: 73 139 891 523 382 1597 293 4545
Fragments arranged by size:
4545 1597 891 523 382 293 139 73
AvrII C'CTAG_G
Cuts at: 5714 5714
Size: 8443
BamHI G'GATC_C
Cuts at: 2940 4145 4734 5292 5773 6140 2940
Size: 1205 589 558 481 367 5243
Fragments arranged by size:
5243 1205 589 558 481 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4203 5150 5206 5264 5438
Size: 325 217 627 2416 947 56 58 174
Cuts at: 5438 5552 5764 7219 618
Size: 114 212 1455 1842
Fragments arranged by size:
2416 1842 1455 947 627 325 217 212
174 114 58 56
BanII G_rGCy'C
Cuts at: 1391 2098 3066 4073 4213 5762 1391
Size: 707 968 1007 140 1549 4072
Fragments arranged by size:
4072 1549 1007 968 707 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 2552 4768 5002 928
Size: 971 377 276 2216 234 4369
Fragments arranged by size:
4369 2216 971 377 276 234
BbvCI CC'TCA_GC
Cuts at: 2847 5631 2847
Size: 2784 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6634 6668 1903
Size: 34 4697 34 3678
Fragments arranged by size:
4697 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2049 6335 7545 2049
Size: 4286 1210 2947
Fragments arranged by size:
4286 2947 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2450 4581 7054
Size: 107 122 71 631 605 778 2131 2473
Cuts at: 7054 136
Size: 1525
Fragments arranged by size:
2473 2131 1525 778 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4019 4506 8438 4019
Size: 487 3932 4024
Fragments arranged by size:
4024 3932 487
BlpI GC'TnA_GC
Cuts at: 2097 2469 5285 5860 2097
Size: 372 2816 575 4680
Fragments arranged by size:
4680 2816 575 372
Bme1580I G_kGCm'C
Cuts at: 1790 2704 5153 5429 6504 7660 1790
Size: 914 2449 276 1075 1156 2573
Fragments arranged by size:
2573 2449 1156 1075 914 276
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4000 4003 4996
Size: 416 53 83 399 24 2583 3 993
Cuts at: 4996 6174 7132 442
Size: 1178 958 1753
Fragments arranged by size:
2583 1753 1178 993 958 416 399 83
53 24 3
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3709 4087 3103
Size: 606 378 7459
Fragments arranged by size:
7459 606 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2536 3466 3566 3892 3938 4412 7103 1239
Size: 1297 930 100 326 46 474 2691 2579
Fragments arranged by size:
2691 2579 1297 930 474 326 100 46
Bpu10I CC'TnA_GC
Cuts at: 2847 4896 5519 5631 2847
Size: 2049 623 112 5659
Fragments arranged by size:
5659 2049 623 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6568 7433 7731 6568
Size: 865 298 7280
Fragments arranged by size:
7280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4799 4929 7106 882
Size: 617 3300 130 2177 2219
Fragments arranged by size:
3300 2219 2177 617 130
BsaAI yAC'GTr
Cuts at: 493 1204 5740 6234 493
Size: 711 4536 494 2702
Fragments arranged by size:
4536 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8437 8437
Size: 8443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2732 6250 6632 7267
Size: 53 83 186 936 1199 3518 382 635
Cuts at: 7267 275
Size: 1451
Fragments arranged by size:
3518 1451 1199 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2052 6876 7274 7548 7695 8106 2052
Size: 4824 398 274 147 411 2389
Fragments arranged by size:
4824 2389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4926 4956 5628 5658 1178
Size: 30 165 30 3523 30 672 30 3963
Fragments arranged by size:
3963 3523 672 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 3787 3962 4079 4091 4108 4831 5443
Size: 1543 1058 175 117 12 17 723 612
Cuts at: 5443 1186
Size: 4186
Fragments arranged by size:
4186 1543 1058 723 612 175 117 17
12
BseYI C'CCAG_C
Cuts at: 3771 3830 3902 4174 4295 4568 5460 5574 5624
Size: 59 72 272 121 273 892 114 50
Cuts at: 5624 7646 8142 3771
Size: 2022 496 4072
Fragments arranged by size:
4072 2022 892 496 273 272 121 114
72 59 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2738 4218 4514 4727 5354 1163
Size: 573 1002 1480 296 213 627 4252
Fragments arranged by size:
4252 1480 1002 627 573 296 213
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3125 5893 6654 7682 1540
Size: 275 186 1124 2768 761 1028 2301
Fragments arranged by size:
2768 2301 1124 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2083 2098 4073 4158 4213 4761 5351 6504 6589
Size: 15 1975 85 55 548 590 1153 85
Cuts at: 6589 7660 2083
Size: 1071 2866
Fragments arranged by size:
2866 1975 1153 1071 590 548 85 85
55 15
BsmI GAATG_Cn'
Cuts at: 2050 3504 5104 5185 5403 5954 6047 2050
Size: 1454 1600 81 218 551 93 4446
Fragments arranged by size:
4446 1600 1454 551 218 93 81
BsmBI CGTCTCn'nnnn_
Cuts at: 3526 4997 3526
Size: 1471 6972
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2098 2704 3066 4073 4158 4213
Size: 399 293 15 606 362 1007 85 55
Cuts at: 4213 4761 5153 5351 5429 5762 6504 6589 7660
Size: 548 392 198 78 333 742 85 1071
Cuts at: 7660 1391
Size: 2174
Fragments arranged by size:
2174 1071 1007 742 606 548 399 392
362 333 293 198 85 85 78 55
15
BspEI T'CCGG_A
Cuts at: 2052 8106 2052
Size: 6054 2389
BspHI T'CATG_A
Cuts at: 4306 6332 8062 4306
Size: 2026 1730 4687
Fragments arranged by size:
4687 2026 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3814 5798 844
Size: 2970 1984 3489
Fragments arranged by size:
3489 2970 1984
BsrBI CCG'CTC
Cuts at: 1394 1823 2772 3201 3309 3883 5044 6330 1394
Size: 429 949 429 108 574 1161 1286 3507
Fragments arranged by size:
3507 1286 1161 949 574 429 429 108
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4225 4404 6938 7112 65
Size: 1183 1191 1786 179 2534 174 1396
Fragments arranged by size:
2534 1786 1396 1191 1183 179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3120 3158 3555 4422 7087 7274 1700
Size: 110 1310 38 397 867 2665 187 2869
Fragments arranged by size:
2869 2665 1310 867 397 187 110 38
BsrGI T'GTAC_A
Cuts at: 96 3052 3408 96
Size: 2956 356 5131
Fragments arranged by size:
5131 2956 356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8443
BssSI C'ACGA_G
Cuts at: 6503 7515 6503
Size: 1012 7431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2961 794
Size: 2167 6276
BstBI TT'CG_AA
Cuts at: 2007 7247 2007
Size: 5240 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8443
BstXI CCAn_nnnn'nTGG
Cuts at: 2936 3916 2936
Size: 980 7463
BstYI r'GATC_y
Cuts at: 1972 2499 2658 2940 3269 4019 4145 4196 4506
Size: 527 159 282 329 750 126 51 310
Cuts at: 4506 4734 5292 5773 6140 6525 6542 7994 8080
Size: 228 558 481 367 385 17 1452 86
Cuts at: 8080 8092 8438 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 750 558 527 481 385 367
346 329 310 282 228 159 126 86
51 17 12
Bsu36I CC'TnA_GG
Cuts at: 4062 5230 4062
Size: 1168 7275
BtgI C'CryG_G
Cuts at: 513 1065 2088 2315 2856 3706 4795 5328 5355
Size: 552 1023 227 541 850 1089 533 27
Cuts at: 5355 5364 5726 513
Size: 9 362 3230
Fragments arranged by size:
3230 1089 1023 850 552 541 533 362
227 27 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7704
Fragments arranged by size:
7704 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 2487 3153 3688 4374 4614 5647 5959 6753
Size: 74 666 535 686 240 1033 312 794
Cuts at: 6753 6773 2413
Size: 20 4083
Fragments arranged by size:
4083 1033 794 686 666 535 312 240
74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8408
DraI TTT'AAA
Cuts at: 5747 5747
Size: 8443
DraIII CAC_nnn'GTG
Cuts at: 4765 4765
Size: 8443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7450 809
Size: 507 6134 1802
Fragments arranged by size:
6134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3122 3709 4395
Size: 54 1425 50 275 18 1292 587 686
Cuts at: 4395 4543 5890 6779 8408 8
Size: 148 1347 889 1629 43
Fragments arranged by size:
1629 1425 1347 1292 889 686 587 275
148 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3122 5890 1537
Size: 275 1310 2768 4090
Fragments arranged by size:
4090 2768 1310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2779 3355 4345 5596 6373 1353
Size: 1426 576 990 1251 777 3423
Fragments arranged by size:
3423 1426 1251 990 777 576
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3332 5324 7030 7404 7550 387
Size: 1064 138 1743 1992 1706 374 146 1280
Fragments arranged by size:
1992 1743 1706 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2096 4071 4211 2096
Size: 1975 140 6328
Fragments arranged by size:
6328 1975 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2536 3118 3399 3466 3503 3545
Size: 393 556 348 582 281 67 37 42
Cuts at: 3545 3566 3892 3938 4412 4762 5096 5153 6506
Size: 21 326 46 474 350 334 57 1353
Cuts at: 6506 7103 7890 8158 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1353 787 597 582 556 474 393
350 348 334 326 281 268 67 57
46 42 37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 4593 4729 4813 4899 5241 4593
Size: 136 84 86 342 7795
Fragments arranged by size:
7795 342 136 86 84
EcoO109I rG'GnC_Cy
Cuts at: 3109 3188 3212 3653 3826 4354 5234 5711 3109
Size: 79 24 441 173 528 880 477 5841
Fragments arranged by size:
5841 880 528 477 441 173 79 24
EcoRI G'AATT_C
Cuts at: 5752 6130 5752
Size: 378 8065
FspI TGC'GCA
Cuts at: 6949 6949
Size: 8443
HaeII r_GCGC'y
Cuts at: 1678 2866 5631 7590 1678
Size: 1188 2765 1959 2531
Fragments arranged by size:
2765 2531 1959 1188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2572 2604 2886 2918 3305
Size: 32 135 32 1000 32 282 32 387
Cuts at: 3305 3337 4239 4271 4370 4402 5628 5660 7084
Size: 32 902 32 99 32 1226 32 1424
Cuts at: 7084 7116 7158 7190 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1424 1226 1000 902 387 282 135
99 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2522 2623 3093 3264 4348 5658
Size: 988 281 584 101 470 171 1084 1310
Cuts at: 5658 5787 6033 669
Size: 129 246 3079
Fragments arranged by size:
3079 1310 1084 988 584 470 281 246
171 129 101
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8443
HpaI GTT'AAC
Cuts at: 2623 4348 6033 2623
Size: 1725 1685 5033
Fragments arranged by size:
5033 1725 1685
KpnI G_GTAC'C
Cuts at: 5556 5768 5556
Size: 212 8231
MfeI C'AATT_G
Cuts at: 2616 6042 2616
Size: 3426 5017
MluI A'CGCG_T
Cuts at: 8319 8319
Size: 8443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2917 3720 5049 7557 7741 1305
Size: 1612 803 1329 2508 184 2007
Fragments arranged by size:
2508 2007 1612 1329 803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3711 4397 4545 10
Size: 54 1425 343 1879 686 148 3908
Fragments arranged by size:
3908 1879 1425 686 343 148 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2786 3681 4311 4679 5135 5354
Size: 648 1071 549 895 630 368 456 219
Cuts at: 5354 6401 6760 6919 518
Size: 1047 359 159 2042
Fragments arranged by size:
2042 1071 1047 895 648 630 549 456
368 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2899 2918 2961 4524 4858 4996 5173
Size: 495 798 19 43 1563 334 138 177
Cuts at: 5173 5338 5859 6536 7684 7929 1606
Size: 165 521 677 1148 245 2120
Fragments arranged by size:
2120 1563 1148 798 677 521 495 334
245 177 165 138 43 19
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 8443
NcoI C'CATG_G
Cuts at: 513 1065 2088 2315 4795 5355 5364 513
Size: 552 1023 227 2480 560 9 3592
Fragments arranged by size:
3592 2480 1023 560 552 227 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8443
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 8443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8443
NotI GC'GGCC_GC
Cuts at: 5890 5890
Size: 8443
NspI r_CATG'y
Cuts at: 1132 1229 2761 4868 5801 7346 1132
Size: 97 1532 2107 933 1545 2229
Fragments arranged by size:
2229 2107 1545 1532 933 97
PasI CC'CwG_GG
Cuts at: 1348 5558 1348
Size: 4210 4233
PciI A'CATG_T
Cuts at: 4864 7342 4864
Size: 2478 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3857 4552 4633 4907 5161 5677 1108
Size: 2749 695 81 274 254 516 3874
Fragments arranged by size:
3874 2749 695 516 274 254 81
PmeI GTTT'AAAC
Cuts at: 5747 5747
Size: 8443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 6512 6544 8025 8057 3293
Size: 32 3187 32 1481 32 3679
Fragments arranged by size:
3679 3187 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3188 3826 4354 5234 3188
Size: 638 528 880 6397
Fragments arranged by size:
6397 880 638 528
PshAI GACnn'nnGTC
Cuts at: 1613 2517 1613
Size: 904 7539
PsiI TTA'TAA
Cuts at: 6013 6013
Size: 8443
PspXI vC'TCGA_Gb
Cuts at: 2973 2973
Size: 8443
PstI C_TGCA'G
Cuts at: 830 2112 4540 5178 5710 5795 830
Size: 1282 2428 638 532 85 3478
Fragments arranged by size:
3478 2428 1282 638 532 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8443
PvuII CAG'CTG
Cuts at: 2918 4524 4996 5173 2918
Size: 1606 472 177 6188
Fragments arranged by size:
6188 1606 472 177
SacI G_AGCT'C
Cuts at: 2098 4073 4213 2098
Size: 1975 140 6328
Fragments arranged by size:
6328 1975 140
SalI G'TCGA_C
Cuts at: 1936 2520 5785 1936
Size: 584 3265 4594
Fragments arranged by size:
4594 3265 584
SapI GCTCTTCn'nnn_
Cuts at: 2779 2779
Size: 8443
SbfI CC_TGCA'GG
Cuts at: 5795 5795
Size: 8443
ScaI AGT'ACT
Cuts at: 3291 4283 4982 6691 8403 3291
Size: 992 699 1709 1712 3331
Fragments arranged by size:
3331 1712 1709 992 699
SexAI A'CCwGG_T
Cuts at: 4938 5434 4938
Size: 496 7947
SfcI C'TryA_G
Cuts at: 826 1045 2108 4536 5174 5706 5791 6926 7607
Size: 219 1063 2428 638 532 85 1135 681
Cuts at: 7607 7798 826
Size: 191 1471
Fragments arranged by size:
2428 1471 1135 1063 681 638 532 219
191 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8443
SmaI CCC'GGG
Cuts at: 1872 5770 1872
Size: 3898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2973 3878 6547 7448 7710 820
Size: 197 926 1030 905 2669 901 262 1553
Fragments arranged by size:
2669 1553 1030 926 905 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5740 6234 493
Size: 5247 494 2702
Fragments arranged by size:
5247 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8443
SphI G_CATG'C
Cuts at: 1132 5801 1132
Size: 4669 3774
SspI AAT'ATT
Cuts at: 5 52 6367 5
Size: 47 6315 2081
Fragments arranged by size:
6315 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 3086 4043 4961 5517 1517
Size: 832 737 957 918 556 4443
Fragments arranged by size:
4443 957 918 832 737 556
StyI C'CwwG_G
Cuts at: 513 1065 2088 2315 2935 4259 4795 5355 5364
Size: 552 1023 227 620 1324 536 560 9
Cuts at: 5364 5714 5882 513
Size: 350 168 3074
Fragments arranged by size:
3074 1324 1023 620 560 552 536 350
227 168 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 3074 4022 4173 5168 6470 6487
Size: 1255 669 825 948 151 995 1302 17
Cuts at: 6487 6640 6825 7270 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1302 1255 995 948 825 669 445
185 153 151 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3052 3137 3289
Size: 274 80 33 51 1443 1075 85 152
Cuts at: 3289 3408 4281 4611 4980 6689 8401 96
Size: 119 873 330 369 1709 1712 138
Fragments arranged by size:
1712 1709 1443 1075 873 369 330 274
152 138 119 85 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2317 2349 2747 2779 2962 2994 4719 4751 4926
Size: 32 398 32 183 32 1725 32 175
Cuts at: 4926 4958 8403 8435 2317
Size: 32 3445 32 2325
Fragments arranged by size:
3445 2325 1725 398 183 175 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3005 1316
Size: 1689 6754
XbaI T'CTAG_A
Cuts at: 5779 5779
Size: 8443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 4759 5362 5678 2446
Size: 2313 603 316 5211
Fragments arranged by size:
5211 2313 603 316
XhoI C'TCGA_G
Cuts at: 1943 2973 3878 1943
Size: 1030 905 6508
Fragments arranged by size:
6508 1030 905
XmaI C'CCGG_G
Cuts at: 1870 5768 1870
Size: 3898 4545
XmnI GAAnn'nnTTC
Cuts at: 6572 6572
Size: 8443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6251 276
Size: 53 83 186 936 4717 2468
Fragments arranged by size:
4717 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI