Restriction map for
pFN21ASDA1734
(Circular) (Six-base) MAPSORT of: pFN21ASDA1734.seq Check: 6026 from: 1 to: 8605
pFN21ASDA1734 8605 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2529 2751 4080 6415
Size: 53 83 186 936 993 222 1329 2335
Cuts at: 6415 278
Size: 2468
Fragments arranged by size:
2468 2335 1329 993 936 222 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2661 3831 5948 1937
Size: 724 1170 2117 4594
Fragments arranged by size:
4594 2117 1170 724
Acc65I G'GTAC_C
Cuts at: 2400 5926 2400
Size: 3526 5079
AclI AA'CG_TT
Cuts at: 1563 6732 7105 1563
Size: 5169 373 3063
Fragments arranged by size:
5169 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 4870 6668 8052 8320 1632
Size: 1076 2162 1798 1384 268 1917
Fragments arranged by size:
2162 1917 1798 1384 1076 268
AfeI AGC'GCT
Cuts at: 3077 3077
Size: 8605
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8408
AflIII A'CryG_T
Cuts at: 1203 7504 8481 1203
Size: 6301 977 1327
Fragments arranged by size:
6301 1327 977
AgeI A'CCGG_T
Cuts at: 7436 7436
Size: 8605
AhdI GACnn_n'nnGTC
Cuts at: 2218 7334 2218
Size: 5116 3489
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2612 1184
Size: 1428 7177
AlwNI CAG_nnn'CTG
Cuts at: 2197 2857 3824 4519 5316 5494 5644 6348 7920
Size: 660 967 695 797 178 150 704 1572
Cuts at: 7920 2197
Size: 2882
Fragments arranged by size:
2882 1572 967 797 704 695 660 178
150
ApaI G_GGCC'C
Cuts at: 4997 4997
Size: 8605
ApaLI G'TGCA_C
Cuts at: 2243 3749 5884 6662 7818 2243
Size: 1506 2135 778 1156 3030
Fragments arranged by size:
3030 2135 1506 1156 778
ApoI r'AATT_y
Cuts at: 1493 5914 6140 6292 8401 1493
Size: 4421 226 152 2109 1697
Fragments arranged by size:
4421 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7159 160
Size: 872 6127 1606
Fragments arranged by size:
6127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8605
AvaI C'yCGr_G
Cuts at: 1870 1943 2317 2469 2770 3243 3627 4566 4697
Size: 73 374 152 301 473 384 939 131
Cuts at: 4697 5381 5930 1870
Size: 684 549 4545
Fragments arranged by size:
4545 939 684 549 473 384 374 301
152 131 73
AvrII C'CTAG_G
Cuts at: 3019 3019
Size: 8605
BamHI G'GATC_C
Cuts at: 2029 5935 6302 2029
Size: 3906 367 4332
Fragments arranged by size:
4332 3906 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2131 2400 2464 2558
Size: 325 217 627 338 6 269 64 94
Cuts at: 2558 2702 2732 5156 5506 5633 5704 5926 7381
Size: 144 30 2424 350 127 71 222 1455
Cuts at: 7381 618
Size: 1842
Fragments arranged by size:
2424 1842 1455 627 350 338 325 269
222 217 144 127 94 71 64 30
6
BanII G_rGCy'C
Cuts at: 1391 2318 3164 3381 4030 4296 4529 4997 5535
Size: 927 846 217 649 266 233 468 538
Cuts at: 5535 5618 5924 1391
Size: 83 306 4072
Fragments arranged by size:
4072 927 846 649 538 468 306 266
233 217 83
BbeI G_GCGC'C
Cuts at: 2129 2135 2706 2736 2129
Size: 6 571 30 7998
Fragments arranged by size:
7998 571 30 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3082 3267 4625 4798 928
Size: 971 1183 185 1358 173 4735
Fragments arranged by size:
4735 1358 1183 971 185 173
BbvCI CC'TCA_GC
Cuts at: 3682 3893 5650 3682
Size: 211 1757 6637
Fragments arranged by size:
6637 1757 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2503 2537 3320 3354 6796 6830 1903
Size: 34 566 34 783 34 3442 34 3678
Fragments arranged by size:
3678 3442 783 566 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6497 7707 6497
Size: 1210 7395
BclI T'GATC_A
Cuts at: 1548 4347 1548
Size: 2799 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2090 4406 4702
Size: 107 122 71 631 605 418 2316 296
Cuts at: 4702 5154 7216 136
Size: 452 2062 1525
Fragments arranged by size:
2316 2062 1525 631 605 452 418 296
122 107 71
BglII A'GATC_T
Cuts at: 2835 8600 2835
Size: 5765 2840
BlpI GC'TnA_GC
Cuts at: 2879 3057 6022 2879
Size: 178 2965 5462
Fragments arranged by size:
5462 2965 178
Bme1580I G_kGCm'C
Cuts at: 1790 2247 2469 2975 3753 4671 4997 5070 5159
Size: 457 222 506 778 918 326 73 89
Cuts at: 5159 5511 5638 5888 6666 7822 1790
Size: 352 127 250 778 1156 2573
Fragments arranged by size:
2573 1156 918 778 778 506 457 352
326 250 222 127 89 73
BmgBI CAC'GTC
Cuts at: 1318 2761 3691 3913 1318
Size: 1443 930 222 6010
Fragments arranged by size:
6010 1443 930 222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3229 5706 6336
Size: 416 53 83 399 24 1812 2477 630
Cuts at: 6336 7294 442
Size: 958 1753
Fragments arranged by size:
2477 1812 1753 958 630 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5658 1056
Size: 4602 4003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4825 4825
Size: 8605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2046 2064 2827 3801 4331 4505 4838 4851
Size: 807 18 763 974 530 174 333 13
Cuts at: 4851 5192 5498 7265 1239
Size: 341 306 1767 2579
Fragments arranged by size:
2579 1767 974 807 763 530 341 333
306 174 18 13
Bpu10I CC'TnA_GC
Cuts at: 3682 3893 5650 3682
Size: 211 1757 6637
Fragments arranged by size:
6637 1757 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4478 4598 6730 7595 7893 4478
Size: 120 2132 865 298 5190
Fragments arranged by size:
5190 2132 865 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2426 2780 5678 7268 882
Size: 617 927 354 2898 1590 2219
Fragments arranged by size:
2898 2219 1590 927 617 354
BsaAI yAC'GTr
Cuts at: 493 1204 2386 5902 6396 493
Size: 711 1182 3516 494 2702
Fragments arranged by size:
3516 2702 1182 711 494
BsaBI GATnn'nnATC
Cuts at: 8599 8599
Size: 8605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 2132 2412 2526
Size: 53 83 186 936 593 6 280 114
Cuts at: 2526 2703 2733 2748 3326 4077 4776 6412 6794
Size: 177 30 15 578 751 699 1636 382
Cuts at: 6794 7429 275
Size: 635 1451
Fragments arranged by size:
1636 1451 936 751 699 635 593 578
382 280 186 177 114 83 53 30
15 6
BsaWI w'CCGG_w
Cuts at: 5745 7038 7436 7710 7857 8268 5745
Size: 1293 398 274 147 411 6082
Fragments arranged by size:
6082 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 2222 2252 3713
Size: 30 165 30 732 30 57 30 1461
Cuts at: 3713 3743 3774 3804 4085 4115 4562 4592 1178
Size: 30 31 30 281 30 447 30 5191
Fragments arranged by size:
5191 1461 732 447 281 165 57 31
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2315 2366 3335 3389 3499 4020 4181
Size: 1126 3 51 969 54 110 521 161
Cuts at: 4181 4304 4661 4664 1186
Size: 123 357 3 5127
Fragments arranged by size:
5127 1126 969 521 357 161 123 110
54 51 3 3
BseYI C'CCAG_C
Cuts at: 2104 2650 3094 4134 4242 4730 5059 5361 5536
Size: 546 444 1040 108 488 329 302 175
Cuts at: 5536 7808 8304 2104
Size: 2272 496 2405
Fragments arranged by size:
2405 2272 1040 546 496 488 444 329
302 175 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2939 3390 3425 5369 1163
Size: 573 1203 451 35 1944 4399
Fragments arranged by size:
4399 1944 1203 573 451 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 6055 6816 7844 1540
Size: 275 186 1864 2190 761 1028 2301
Fragments arranged by size:
2301 2190 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2318 2327 2714 2820 2880 3753 4278 5217
Size: 71 9 387 106 60 873 525 939
Cuts at: 5217 5888 6666 6751 7822 2247
Size: 671 778 85 1071 3030
Fragments arranged by size:
3030 1071 939 873 778 671 525 387
106 85 71 60 9
BsmI GAATG_Cn'
Cuts at: 3722 6116 6209 3722
Size: 2394 93 6118
Fragments arranged by size:
6118 2394 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3041 3317 4241 3041
Size: 276 924 7405
Fragments arranged by size:
7405 924 276
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2318 2327 2469 2714 2820 2880
Size: 399 457 71 9 142 245 106 60
Cuts at: 2880 2975 3164 3381 3753 4030 4278 4296 4529
Size: 95 189 217 372 277 248 18 233
Cuts at: 4529 4671 4997 5070 5159 5217 5511 5535 5618
Size: 142 326 73 89 58 294 24 83
Cuts at: 5618 5638 5888 5924 6666 6751 7822 1391
Size: 20 250 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 399 372 326 294
277 250 248 245 233 217 189 142
142 106 95 89 85 83 73 71
60 58 36 24 20 18 9
BspEI T'CCGG_A
Cuts at: 8268 8268
Size: 8605
BspHI T'CATG_A
Cuts at: 6494 8224 6494
Size: 1730 6875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 2911 3190 3878 5960 844
Size: 1760 307 279 688 2082 3489
Fragments arranged by size:
3489 2082 1760 688 307 279
BsrBI CCG'CTC
Cuts at: 1394 1823 2060 3540 3729 6492 1394
Size: 429 237 1480 189 2763 3507
Fragments arranged by size:
3507 2763 1480 429 237 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 2631 3954 7100 7274 65
Size: 1183 1383 1323 3146 174 1396
Fragments arranged by size:
3146 1396 1383 1323 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2128 2183 4318 5267 7249 7436 1700
Size: 110 318 55 2135 949 1982 187 2869
Fragments arranged by size:
2869 2135 1982 949 318 187 110 55
BsrGI T'GTAC_A
Cuts at: 96 4143 96
Size: 4047 4558
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8605
BssSI C'ACGA_G
Cuts at: 2348 3383 3550 6665 7677 2348
Size: 1035 167 3115 1012 3276
Fragments arranged by size:
3276 3115 1035 1012 167
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2197 2565 3205 794
Size: 1403 368 640 6194
Fragments arranged by size:
6194 1403 640 368
BstBI TT'CG_AA
Cuts at: 2007 7409 2007
Size: 5402 3203
BstEII G'GTnAC_C
Cuts at: 1184 3502 3667 1184
Size: 2318 165 6122
Fragments arranged by size:
6122 2318 165
BstXI CCAn_nnnn'nTGG
Cuts at: 3349 4760 5099 3349
Size: 1411 339 6855
Fragments arranged by size:
6855 1411 339
BstYI r'GATC_y
Cuts at: 1972 2029 2835 4479 5935 6302 6687 6704 8156
Size: 57 806 1644 1456 367 385 17 1452
Cuts at: 8156 8242 8254 8600 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1644 1456 1452 806 385 367 346
86 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 3318 4329 4932 5577 3318
Size: 1011 603 645 6346
Fragments arranged by size:
6346 1011 645 603
BtgI C'CryG_G
Cuts at: 513 1065 2022 2233 2269 2308 2377 3255 3711
Size: 552 957 211 36 39 69 878 456
Cuts at: 3711 4392 4404 513
Size: 681 12 4714
Fragments arranged by size:
4714 957 878 681 552 456 211 69
39 36 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7866
Fragments arranged by size:
7866 683 56
BtsI GCAGTG_nn'
Cuts at: 3663 4671 4763 4925 5104 5492 6121 6915 6935
Size: 1008 92 162 179 388 629 794 20
Cuts at: 6935 3663
Size: 5333
Fragments arranged by size:
5333 1008 794 629 388 179 162 92
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8570
DraI TTT'AAA
Cuts at: 5909 5909
Size: 8605
DraIII CAC_nnn'GTG
Cuts at: 3237 5714 3237
Size: 2477 6128
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2543 3827 5194 7612 809
Size: 507 1227 1284 1367 2418 1802
Fragments arranged by size:
2418 1802 1367 1284 1227 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3168 3862 4389
Size: 54 1425 50 275 18 1338 694 527
Cuts at: 4389 4407 4635 5101 5563 6052 6941 8570 8
Size: 18 228 466 462 489 889 1629 43
Fragments arranged by size:
1629 1425 1338 889 694 527 489 466
462 275 228 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 6052 1537
Size: 275 2050 2190 4090
Fragments arranged by size:
4090 2190 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 2907 3746 3924 4350 4979 5759 5801
Size: 1430 124 839 178 426 629 780 42
Cuts at: 5801 6535 1353
Size: 734 3423
Fragments arranged by size:
3423 1430 839 780 734 629 426 178
124 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 7192 7566 7712 387
Size: 1064 138 453 5150 374 146 1280
Fragments arranged by size:
5150 1280 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 2316 2316
Size: 8605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2064 2708 2827 3801 4331 4505
Size: 393 414 18 644 119 974 530 174
Cuts at: 4505 4838 4851 4870 5192 5498 6668 7265 8052
Size: 333 13 19 322 306 1170 597 787
Cuts at: 8052 8320 1239
Size: 268 1524
Fragments arranged by size:
1524 1170 974 787 644 597 530 414
393 333 322 306 268 174 119 19
18 13
EcoNI CCTnn'n_nnAGG
Cuts at: 5207 5864 5207
Size: 657 7948
EcoO109I rG'GnC_Cy
Cuts at: 2071 2593 4993 4994 2071
Size: 522 2400 1 5682
Fragments arranged by size:
5682 2400 522 1
EcoRI G'AATT_C
Cuts at: 5914 6292 5914
Size: 378 8227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4900 4900
Size: 8605
FspI TGC'GCA
Cuts at: 7111 7111
Size: 8605
HaeII r_GCGC'y
Cuts at: 1678 2129 2135 2197 2706 2736 3079 3187 4794
Size: 451 6 62 509 30 343 108 1607
Cuts at: 4794 4913 5336 7752 1678
Size: 119 423 2416 2531
Fragments arranged by size:
2531 2416 1607 509 451 423 343 119
108 62 30 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2204 2236 2270
Size: 32 135 32 563 32 37 32 34
Cuts at: 2270 2302 2463 2495 2947 2979 4034 4066 4332
Size: 32 161 32 452 32 1055 32 266
Cuts at: 4332 4364 4691 4723 4961 4993 7246 7278 7320
Size: 32 327 32 238 32 2253 32 42
Cuts at: 7320 7352 1373
Size: 32 2626
Fragments arranged by size:
2626 2253 1055 563 452 327 266 238
161 135 42 37 34 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 2584 5949 6195 669
Size: 988 281 592 54 3365 246 3079
Fragments arranged by size:
3365 3079 988 592 281 246 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8605
HpaI GTT'AAC
Cuts at: 6195 6195
Size: 8605
KasI G'GCGC_C
Cuts at: 2125 2131 2702 2732 2125
Size: 6 571 30 7998
Fragments arranged by size:
7998 571 30 6
KpnI G_GTAC'C
Cuts at: 2404 5930 2404
Size: 3526 5079
MfeI C'AATT_G
Cuts at: 6204 6204
Size: 8605
MluI A'CGCG_T
Cuts at: 8481 8481
Size: 8605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2721 3756 4565 7719 7903 1305
Size: 1416 1035 809 3154 184 2007
Fragments arranged by size:
3154 2007 1416 1035 809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3170 4391 4637 10
Size: 54 1425 343 1338 1221 246 3978
Fragments arranged by size:
3978 1425 1338 1221 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4505 6563 6922 7081 518
Size: 648 3339 2058 359 159 2042
Fragments arranged by size:
3339 2058 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2310 2379 3061 3400 3546 4203 4242 4370
Size: 704 69 682 339 146 657 39 128
Cuts at: 4370 4516 5365 5540 6021 6698 7846 8091 1606
Size: 146 849 175 481 677 1148 245 2120
Fragments arranged by size:
2120 1148 849 704 682 677 657 481
339 245 175 146 146 128 69 39
NaeI GCC'GGC
Cuts at: 2130 2185 5269 2130
Size: 55 3084 5466
Fragments arranged by size:
5466 3084 55
NarI GG'CG_CC
Cuts at: 2126 2132 2703 2733 2126
Size: 6 571 30 7998
Fragments arranged by size:
7998 571 30 6
NcoI C'CATG_G
Cuts at: 513 1065 2022 3255 4392 4404 513
Size: 552 957 1233 1137 12 4714
Fragments arranged by size:
4714 1233 1137 957 552 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8605
NgoMIV G'CCGG_C
Cuts at: 2128 2183 5267 2128
Size: 55 3084 5466
Fragments arranged by size:
5466 3084 55
NheI G'CTAG_C
Cuts at: 1052 5654 1052
Size: 4602 4003
NotI GC'GGCC_GC
Cuts at: 6052 6052
Size: 8605
NspI r_CATG'y
Cuts at: 1132 1229 2426 2491 5878 5963 7508 1132
Size: 97 1197 65 3387 85 1545 2229
Fragments arranged by size:
3387 2229 1545 1197 97 85 65
PasI CC'CwG_GG
Cuts at: 1348 3151 5611 1348
Size: 1803 2460 4342
Fragments arranged by size:
4342 2460 1803
PciI A'CATG_T
Cuts at: 7504 7504
Size: 8605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3256 5644 1108
Size: 2148 2388 4069
Fragments arranged by size:
4069 2388 2148
PfoI T'CCnGG_A
Cuts at: 4455 4482 4455
Size: 27 8578
PmeI GTTT'AAAC
Cuts at: 5909 5909
Size: 8605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6674 6706 8187 8219 6674
Size: 32 1481 32 7060
Fragments arranged by size:
7060 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2593 2593
Size: 8605
PshAI GACnn'nnGTC
Cuts at: 1613 2772 1613
Size: 1159 7446
PsiI TTA'TAA
Cuts at: 6175 6175
Size: 8605
PspOMI G'GGCC_C
Cuts at: 4993 4993
Size: 8605
PstI C_TGCA'G
Cuts at: 830 2601 5295 5957 830
Size: 1771 2694 662 3478
Fragments arranged by size:
3478 2694 1771 662
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8605
PvuII CAG'CTG
Cuts at: 3400 4242 4370 4516 5365 5540 3400
Size: 842 128 146 849 175 6465
Fragments arranged by size:
6465 849 842 175 146 128
SacI G_AGCT'C
Cuts at: 2318 2318
Size: 8605
SacII CC_GC'GG
Cuts at: 2311 2380 2311
Size: 69 8536
SalI G'TCGA_C
Cuts at: 1936 5947 1936
Size: 4011 4594
SbfI CC_TGCA'GG
Cuts at: 5295 5957 5295
Size: 662 7943
ScaI AGT'ACT
Cuts at: 6853 8565 6853
Size: 1712 6893
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 8605
SfcI C'TryA_G
Cuts at: 826 1045 2597 4601 5291 5541 5953 7088 7769
Size: 219 1552 2004 690 250 412 1135 681
Cuts at: 7769 7960 826
Size: 191 1471
Fragments arranged by size:
2004 1552 1471 1135 690 681 412 250
219 191
SfoI GGC'GCC
Cuts at: 2127 2133 2704 2734 2127
Size: 6 571 30 7998
Fragments arranged by size:
7998 571 30 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8605
SgrAI Cr'CCGG_yG
Cuts at: 1700 2128 1700
Size: 428 8177
SmaI CCC'GGG
Cuts at: 1872 3245 5932 1872
Size: 1373 2687 4545
Fragments arranged by size:
4545 2687 1373
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3627 4493 4613 6709 7610 7872
Size: 197 926 1684 866 120 2096 901 262
Cuts at: 7872 820
Size: 1553
Fragments arranged by size:
2096 1684 1553 926 901 866 262 197
120
SnaBI TAC'GTA
Cuts at: 493 5902 6396 493
Size: 5409 494 2702
Fragments arranged by size:
5409 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8605
SphI G_CATG'C
Cuts at: 1132 5878 5963 1132
Size: 4746 85 3774
Fragments arranged by size:
4746 3774 85
SspI AAT'ATT
Cuts at: 5 52 6529 5
Size: 47 6477 2081
Fragments arranged by size:
6477 2081 47
StuI AGG'CCT
Cuts at: 1517 5289 5606 1517
Size: 3772 317 4516
Fragments arranged by size:
4516 3772 317
StyI C'CwwG_G
Cuts at: 513 1065 2022 3019 3099 3255 4392 4404 4794
Size: 552 957 997 80 156 1137 12 390
Cuts at: 4794 5227 5511 5808 6044 513
Size: 433 284 297 236 3074
Fragments arranged by size:
3074 1137 997 957 552 433 390 297
284 236 156 80 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3023 3248 4459 5298 5725 6632 6649
Size: 1255 1443 225 1211 839 427 907 17
Cuts at: 6649 6802 6987 7432 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1443 1255 1211 907 839 445 427
225 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4143 6851 8563
Size: 274 80 33 51 1443 2166 2708 1712
Cuts at: 8563 96
Size: 138
Fragments arranged by size:
2708 2166 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 3774 3806 4085 4117 5125 5157 8565
Size: 32 1265 32 279 32 1008 32 3408
Cuts at: 8565 8597 2477
Size: 32 2485
Fragments arranged by size:
3408 2485 1265 1008 279 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2981 3995 1316
Size: 1665 1014 5926
Fragments arranged by size:
5926 1665 1014
XbaI T'CTAG_A
Cuts at: 5941 5941
Size: 8605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2819 4306 4480 5362 2819
Size: 1487 174 882 6062
Fragments arranged by size:
6062 1487 882 174
XhoI C'TCGA_G
Cuts at: 1943 3627 1943
Size: 1684 6921
XmaI C'CCGG_G
Cuts at: 1870 3243 5930 1870
Size: 1373 2687 4545
Fragments arranged by size:
4545 2687 1373
XmnI GAAnn'nnTTC
Cuts at: 3884 4883 6734 3884
Size: 999 1851 5755
Fragments arranged by size:
5755 1851 999
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2527 2749 4078 6413
Size: 53 83 186 936 993 222 1329 2335
Cuts at: 6413 276
Size: 2468
Fragments arranged by size:
2468 2335 1329 993 936 222 186 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SapI SfiI SrfI SwaI