Restriction map for
pFN21ASDA1721
(Circular) (Six-base) MAPSORT of: pFN21ASDA1721.seq Check: 7811 from: 1 to: 8182
pFN21ASDA1721 8182 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5992 278
Size: 53 83 186 936 4456 2468
Fragments arranged by size:
4456 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3216 3499 5525 1937
Size: 1279 283 2026 4594
Fragments arranged by size:
4594 2026 1279 283
Acc65I G'GTAC_C
Cuts at: 5503 5503
Size: 8182
AclI AA'CG_TT
Cuts at: 1563 6309 6682 1563
Size: 4746 373 3063
Fragments arranged by size:
4746 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2667 3060 3765 4334 4343 6245 7629 7897
Size: 1035 393 705 569 9 1902 1384 268
Cuts at: 7897 1632
Size: 1917
Fragments arranged by size:
1917 1902 1384 1035 705 569 393 268
9
AflII C'TTAA_G
Cuts at: 820 1017 4372 5183 820
Size: 197 3355 811 3819
Fragments arranged by size:
3819 3355 811 197
AflIII A'CryG_T
Cuts at: 1203 5259 7081 8058 1203
Size: 4056 1822 977 1327
Fragments arranged by size:
4056 1822 1327 977
AgeI A'CCGG_T
Cuts at: 7013 7013
Size: 8182
AhdI GACnn_n'nnGTC
Cuts at: 6911 6911
Size: 8182
AleI CACnn'nnGTG
Cuts at: 1166 4151 5264 1166
Size: 2985 1113 4084
Fragments arranged by size:
4084 2985 1113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2934 4134 1184
Size: 1750 1200 5232
Fragments arranged by size:
5232 1750 1200
AlwNI CAG_nnn'CTG
Cuts at: 3830 4574 5925 7497 3830
Size: 744 1351 1572 4515
Fragments arranged by size:
4515 1572 1351 744
ApaI G_GGCC'C
Cuts at: 2046 2046
Size: 8182
ApaLI G'TGCA_C
Cuts at: 6239 7395 6239
Size: 1156 7026
ApoI r'AATT_y
Cuts at: 1493 2519 2564 2746 3367 3771 4204 4796 5438
Size: 1026 45 182 621 404 433 592 642
Cuts at: 5438 5491 5717 5869 7978 1493
Size: 53 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 642 621 592 433 404
226 182 152 53 45
AseI AT'TA_AT
Cuts at: 160 1032 3756 4970 6736 160
Size: 872 2724 1214 1766 1606
Fragments arranged by size:
2724 1766 1606 1214 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8182
AvaI C'yCGr_G
Cuts at: 1870 1943 3879 5507 1870
Size: 73 1936 1628 4545
Fragments arranged by size:
4545 1936 1628 73
BamHI G'GATC_C
Cuts at: 5512 5879 5512
Size: 367 7815
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 5503 6958 618
Size: 325 217 627 305 3411 1455 1842
Fragments arranged by size:
3411 1842 1455 627 325 305 217
BanII G_rGCy'C
Cuts at: 1391 2046 5501 1391
Size: 655 3455 4072
Fragments arranged by size:
4072 3455 655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2607 2673 928
Size: 971 708 66 6437
Fragments arranged by size:
6437 971 708 66
BbvCI CC'TCA_GC
Cuts at: 4470 4470
Size: 8182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6373 6407 1903
Size: 34 4436 34 3678
Fragments arranged by size:
4436 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6074 7284 6074
Size: 1210 6972
BclI T'GATC_A
Cuts at: 1548 3448 3994 1548
Size: 1900 546 5736
Fragments arranged by size:
5736 1900 546
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6793 136
Size: 107 122 71 631 605 5121 1525
Fragments arranged by size:
5121 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2613 3895 4862 8177 2613
Size: 1282 967 3315 2618
Fragments arranged by size:
3315 2618 1282 967
BlpI GC'TnA_GC
Cuts at: 2061 4212 5599 2061
Size: 2151 1387 4644
Fragments arranged by size:
4644 2151 1387
Bme1580I G_kGCm'C
Cuts at: 1790 2046 6243 7399 1790
Size: 256 4197 1156 2573
Fragments arranged by size:
4197 2573 1156 256
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8182
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2917 5324 5913
Size: 416 53 83 399 24 1500 2407 589
Cuts at: 5913 6871 442
Size: 958 1753
Fragments arranged by size:
2407 1753 1500 958 589 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3914
Size: 8182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 3040 3336 3693 3768 4120 5120 6842
Size: 848 953 296 357 75 352 1000 1722
Cuts at: 6842 1239
Size: 2579
Fragments arranged by size:
2579 1722 1000 953 848 357 352 296
75
Bpu10I CC'TnA_GC
Cuts at: 4470 4470
Size: 8182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2743 6307 7172 7470 2743
Size: 3564 865 298 3455
Fragments arranged by size:
3564 3455 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3429 5465 6845 882
Size: 617 1930 2036 1380 2219
Fragments arranged by size:
2219 2036 1930 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 5479 5973 493
Size: 711 4275 494 2702
Fragments arranged by size:
4275 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8176 8176
Size: 8182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5989 6371 7006 275
Size: 53 83 186 936 4456 382 635 1451
Fragments arranged by size:
4456 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6615 7013 7287 7434 7845 6615
Size: 398 274 147 411 6952
Fragments arranged by size:
6952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3236 3266 1178
Size: 30 165 30 1833 30 6094
Fragments arranged by size:
6094 1833 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3118 3413 5376 1186
Size: 1932 295 1963 3992
Fragments arranged by size:
3992 1963 1932 295
BseYI C'CCAG_C
Cuts at: 2038 3954 7385 7881 2038
Size: 1916 3431 496 2339
Fragments arranged by size:
3431 2339 1916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4154 4283 1163
Size: 573 2418 129 5062
Fragments arranged by size:
5062 2418 573 129
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5632 6393 7421 1540
Size: 275 186 3631 761 1028 2301
Fragments arranged by size:
3631 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2139 6243 6328 7399 2139
Size: 4104 85 1071 2922
Fragments arranged by size:
4104 2922 1071 85
BsmI GAATG_Cn'
Cuts at: 3474 3736 5693 5786 3474
Size: 262 1957 93 5870
Fragments arranged by size:
5870 1957 262 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2139 5501 6243 6328 7399 1391
Size: 399 256 93 3362 742 85 1071 2174
Fragments arranged by size:
3362 2174 1071 742 399 256 93 85
BspEI T'CCGG_A
Cuts at: 7845 7845
Size: 8182
BspHI T'CATG_A
Cuts at: 2437 6071 7801 2437
Size: 3634 1730 2818
Fragments arranged by size:
3634 2818 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4761 4791 5537 844
Size: 3917 30 746 3489
Fragments arranged by size:
3917 3489 746 30
BsrBI CCG'CTC
Cuts at: 1394 1823 6069 1394
Size: 429 4246 3507
Fragments arranged by size:
4246 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4464 6677 6851 65
Size: 1183 3216 2213 174 1396
Fragments arranged by size:
3216 2213 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6826 7013 1700
Size: 110 5016 187 2869
Fragments arranged by size:
5016 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8182
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8182
BssSI C'ACGA_G
Cuts at: 3393 6242 7254 3393
Size: 2849 1012 4321
Fragments arranged by size:
4321 2849 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2791 794
Size: 1997 6185
BstBI TT'CG_AA
Cuts at: 2007 6986 2007
Size: 4979 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8182
BstXI CCAn_nnnn'nTGG
Cuts at: 3385 3385
Size: 8182
BstYI r'GATC_y
Cuts at: 1972 2613 3051 3779 3895 4862 4927 5023 5512
Size: 641 438 728 116 967 65 96 489
Cuts at: 5512 5879 6264 6281 7733 7819 7831 8177 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 967 728 641 489 438 385
367 346 116 96 86 65 17 12
BstZ17I GTA'TAC
Cuts at: 3217 3500 3217
Size: 283 7899
Bsu36I CC'TnA_GG
Cuts at: 5019 5019
Size: 8182
BtgI C'CryG_G
Cuts at: 513 1065 2103 2569 3544 513
Size: 552 1038 466 975 5151
Fragments arranged by size:
5151 1038 975 552 466
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7443
Fragments arranged by size:
7443 683 56
BtsI GCAGTG_nn'
Cuts at: 2918 4437 5235 5370 5698 6492 6512 2918
Size: 1519 798 135 328 794 20 4588
Fragments arranged by size:
4588 1519 798 794 328 135 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8147
DraI TTT'AAA
Cuts at: 3948 5032 5486 3948
Size: 1084 454 6644
Fragments arranged by size:
6644 1084 454
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3546 7189 809
Size: 507 2230 3643 1802
Fragments arranged by size:
3643 2230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3456 5629 6518
Size: 54 1425 50 275 18 1626 2173 889
Cuts at: 6518 8147 8
Size: 1629 43
Fragments arranged by size:
2173 1629 1626 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5629 1537
Size: 275 3817 4090
Fragments arranged by size:
4090 3817 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 2940 3510 4156 4731 6112 1353
Size: 1479 108 570 646 575 1381 3423
Fragments arranged by size:
3423 1479 1381 646 575 570 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6769 7143 7289 387
Size: 1064 138 5180 374 146 1280
Fragments arranged by size:
5180 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2667 3040 3060 3336 3693 3765
Size: 393 455 580 373 20 276 357 72
Cuts at: 3765 3768 4120 4334 4343 5120 6245 6842 7629
Size: 3 352 214 9 777 1125 597 787
Cuts at: 7629 7897 1239
Size: 268 1524
Fragments arranged by size:
1524 1125 787 777 597 580 455 393
373 357 352 276 268 214 72 20
9 3
EcoO109I rG'GnC_Cy
Cuts at: 2042 2043 4114 4453 2042
Size: 1 2071 339 5771
Fragments arranged by size:
5771 2071 339 1
EcoRI G'AATT_C
Cuts at: 2564 4796 5491 5869 2564
Size: 2232 695 378 4877
Fragments arranged by size:
4877 2232 695 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2621 3212 4685 4772 5434 2621
Size: 591 1473 87 662 5369
Fragments arranged by size:
5369 1473 662 591 87
FspI TGC'GCA
Cuts at: 6688 6688
Size: 8182
HaeII r_GCGC'y
Cuts at: 1678 2039 7329 1678
Size: 361 5290 2531
Fragments arranged by size:
5290 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2948 2980 4267 4299 4564
Size: 32 135 32 1376 32 1287 32 265
Cuts at: 4564 4596 4612 4644 6823 6855 6897 6929 1373
Size: 32 16 32 2179 32 42 32 2626
Fragments arranged by size:
2626 2179 1376 1287 265 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 3139 3255 3604 3751 5526
Size: 988 281 687 514 116 349 147 1775
Cuts at: 5526 5772 669
Size: 246 3079
Fragments arranged by size:
3079 1775 988 687 514 349 281 246
147 116
HindIII A'AGCT_T
Cuts at: 748 5250 748
Size: 4502 3680
HpaI GTT'AAC
Cuts at: 2625 3139 3255 5772 2625
Size: 514 116 2517 5035
Fragments arranged by size:
5035 2517 514 116
KpnI G_GTAC'C
Cuts at: 5507 5507
Size: 8182
MfeI C'AATT_G
Cuts at: 2415 4531 5781 2415
Size: 2116 1250 4816
Fragments arranged by size:
4816 2116 1250
MluI A'CGCG_T
Cuts at: 8058 8058
Size: 8182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3222 4967 7296 7480 1305
Size: 1917 1745 2329 184 2007
Fragments arranged by size:
2329 2007 1917 1745 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3458 10
Size: 54 1425 343 1626 4734
Fragments arranged by size:
4734 1626 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4151 5228 5264 6140 6499 6658 518
Size: 648 2985 1077 36 876 359 159 2042
Fragments arranged by size:
2985 2042 1077 876 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 2065 4877 5598 6275 7423 7668 1606
Size: 459 2812 721 677 1148 245 2120
Fragments arranged by size:
2812 2120 1148 721 677 459 245
NcoI C'CATG_G
Cuts at: 513 1065 2103 2569 3544 513
Size: 552 1038 466 975 5151
Fragments arranged by size:
5151 1038 975 552 466
NdeI CA'TA_TG
Cuts at: 387 3229 4308 387
Size: 2842 1079 4261
Fragments arranged by size:
4261 2842 1079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8182
NotI GC'GGCC_GC
Cuts at: 5629 5629
Size: 8182
NspI r_CATG'y
Cuts at: 1132 1229 2132 3308 5540 7085 1132
Size: 97 903 1176 2232 1545 2229
Fragments arranged by size:
2232 2229 1545 1176 903 97
PasI CC'CwG_GG
Cuts at: 1348 3809 1348
Size: 2461 5721
PciI A'CATG_T
Cuts at: 7081 7081
Size: 8182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8182
PfoI T'CCnGG_A
Cuts at: 3784 3784
Size: 8182
PmeI GTTT'AAAC
Cuts at: 5486 5486
Size: 8182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6251 6283 7764 7796 6251
Size: 32 1481 32 6637
Fragments arranged by size:
6637 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4114 4114
Size: 8182
PshAI GACnn'nnGTC
Cuts at: 1613 2607 1613
Size: 994 7188
PsiI TTA'TAA
Cuts at: 5752 5752
Size: 8182
PspOMI G'GGCC_C
Cuts at: 2042 2042
Size: 8182
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2352 2384 3483 3515 2352
Size: 32 1099 32 7019
Fragments arranged by size:
7019 1099 32 32
PstI C_TGCA'G
Cuts at: 830 2643 2913 3490 5534 830
Size: 1813 270 577 2044 3478
Fragments arranged by size:
3478 2044 1813 577 270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8182
PvuII CAG'CTG
Cuts at: 2065 2065
Size: 8182
SalI G'TCGA_C
Cuts at: 1936 5524 1936
Size: 3588 4594
SapI GCTCTTCn'nnn_
Cuts at: 4731 4731
Size: 8182
SbfI CC_TGCA'GG
Cuts at: 5534 5534
Size: 8182
ScaI AGT'ACT
Cuts at: 4961 6430 8142 4961
Size: 1469 1712 5001
Fragments arranged by size:
5001 1712 1469
SfcI C'TryA_G
Cuts at: 826 1045 2639 2909 3486 5530 6665 7346 7537
Size: 219 1594 270 577 2044 1135 681 191
Cuts at: 7537 826
Size: 1471
Fragments arranged by size:
2044 1594 1471 1135 681 577 270 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8182
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8182
SmaI CCC'GGG
Cuts at: 1872 5509 1872
Size: 3637 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2722 3879 4372 5183 6286 7187
Size: 197 926 779 1157 493 811 1103 901
Cuts at: 7187 7449 820
Size: 262 1553
Fragments arranged by size:
1553 1157 1103 926 901 811 779 493
262 197
SnaBI TAC'GTA
Cuts at: 493 5479 5973 493
Size: 4986 494 2702
Fragments arranged by size:
4986 2702 494
SpeI A'CTAG_T
Cuts at: 152 2201 152
Size: 2049 6133
SphI G_CATG'C
Cuts at: 1132 2132 5540 1132
Size: 1000 3408 3774
Fragments arranged by size:
3774 3408 1000
SspI AAT'ATT
Cuts at: 5 52 3725 5288 6106 5
Size: 47 3673 1563 818 2081
Fragments arranged by size:
3673 2081 1563 818 47
StuI AGG'CCT
Cuts at: 1517 5105 1517
Size: 3588 4594
StyI C'CwwG_G
Cuts at: 513 1065 2103 2569 3544 5621 513
Size: 552 1038 466 975 2077 3074
Fragments arranged by size:
3074 2077 1038 975 552 466
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6209 6226 6379 6564 7009 325
Size: 1255 4629 17 153 185 445 1498
Fragments arranged by size:
4629 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2863 4016 4959
Size: 274 80 33 51 1443 886 1153 943
Cuts at: 4959 5043 6428 8140 96
Size: 84 1385 1712 138
Fragments arranged by size:
1712 1443 1385 1153 943 886 274 138
84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8142 8174 8142
Size: 32 8150
Tth111I GACn'n_nGTC
Cuts at: 1316 2216 1316
Size: 900 7282
XbaI T'CTAG_A
Cuts at: 5518 5518
Size: 8182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 2811 2242
Size: 569 7613
XhoI C'TCGA_G
Cuts at: 1943 3879 1943
Size: 1936 6246
XmaI C'CCGG_G
Cuts at: 1870 5507 1870
Size: 3637 4545
XmnI GAAnn'nnTTC
Cuts at: 2364 2604 3636 3826 6311 2364
Size: 240 1032 190 2485 4235
Fragments arranged by size:
4235 2485 1032 240 190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5990 276
Size: 53 83 186 936 4456 2468
Fragments arranged by size:
4456 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI