Restriction map for
pFN21ASDA1716
(Circular) (Six-base) MAPSORT of: pFN21ASDA1716.seq Check: 3107 from: 1 to: 7006
pFN21ASDA1716 7006 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4248 4248
Size: 7006
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4816 278
Size: 53 83 186 936 3280 2468
Fragments arranged by size:
3280 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2793 4349 1937
Size: 856 1556 4594
Fragments arranged by size:
4594 1556 856
Acc65I G'GTAC_C
Cuts at: 4327 4327
Size: 7006
AclI AA'CG_TT
Cuts at: 1563 5133 5506 1563
Size: 3570 373 3063
Fragments arranged by size:
3570 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4085 5069 6453 6721 1632
Size: 2453 984 1384 268 1917
Fragments arranged by size:
2453 1917 1384 984 268
AfeI AGC'GCT
Cuts at: 3328 3328
Size: 7006
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6809
AflIII A'CryG_T
Cuts at: 1203 5905 6882 1203
Size: 4702 977 1327
Fragments arranged by size:
4702 1327 977
AgeI A'CCGG_T
Cuts at: 5837 5837
Size: 7006
AhdI GACnn_n'nnGTC
Cuts at: 5735 5735
Size: 7006
AleI CACnn'nnGTG
Cuts at: 1166 2825 1166
Size: 1659 5347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7006
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 4235 4267 3605
Size: 32 598 32 6344
Fragments arranged by size:
6344 598 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3698 4749 6321 3698
Size: 1051 1572 4383
Fragments arranged by size:
4383 1572 1051
ApaI G_GGCC'C
Cuts at: 2365 2365
Size: 7006
ApaLI G'TGCA_C
Cuts at: 5063 6219 5063
Size: 1156 5850
ApoI r'AATT_y
Cuts at: 1493 4315 4541 4693 6802 1493
Size: 2822 226 152 2109 1697
Fragments arranged by size:
2822 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5560 160
Size: 872 4528 1606
Fragments arranged by size:
4528 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
AvaI C'yCGr_G
Cuts at: 1870 1943 2323 3081 3799 4216 4331 1870
Size: 73 380 758 718 417 115 4545
Fragments arranged by size:
4545 758 718 417 380 115 73
BamHI G'GATC_C
Cuts at: 4336 4703 4336
Size: 367 6639
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2328 3457 3466 3605 4009
Size: 325 217 627 541 1129 9 139 404
Cuts at: 4009 4220 4327 5782 618
Size: 211 107 1455 1842
Fragments arranged by size:
1842 1455 1129 627 541 404 325 217
211 139 107 9
BanII G_rGCy'C
Cuts at: 1391 2365 3005 3570 3807 3941 4248 4325 1391
Size: 974 640 565 237 134 307 77 4072
Fragments arranged by size:
4072 974 640 565 307 237 134 77
BbeI G_GCGC'C
Cuts at: 3461 4013 4224 3461
Size: 552 211 6243
Fragments arranged by size:
6243 552 211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6035
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2047 2081 3824 3858 5197 5231 1903
Size: 34 110 34 1743 34 1339 34 3678
Fragments arranged by size:
3678 1743 1339 110 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 4898 6108 4274
Size: 624 1210 5172
Fragments arranged by size:
5172 1210 624
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7006
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2360 3150
Size: 107 122 71 631 605 367 321 790
Cuts at: 3150 3479 3695 3869 4044 5617 136
Size: 329 216 174 175 1573 1525
Fragments arranged by size:
1573 1525 790 631 605 367 329 321
216 175 174 122 107 71
BglII A'GATC_T
Cuts at: 3339 7001 3339
Size: 3662 3344
BlpI GC'TnA_GC
Cuts at: 4423 4423
Size: 7006
Bme1580I G_kGCm'C
Cuts at: 1790 2365 3608 3832 5067 6223 1790
Size: 575 1243 224 1235 1156 2573
Fragments arranged by size:
2573 1243 1235 1156 575 224
BmgBI CAC'GTC
Cuts at: 1318 2683 3784 3982 1318
Size: 1365 1101 198 4342
Fragments arranged by size:
4342 1365 1101 198
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 2688 3346
Size: 416 53 83 399 24 1062 209 658
Cuts at: 3346 3892 4737 5695 442
Size: 546 845 958 1753
Fragments arranged by size:
1753 1062 958 845 658 546 416 399
209 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3676 3653
Size: 23 6983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2312 2399 3497 3514 4232 5666 1239
Size: 962 111 87 1098 17 718 1434 2579
Fragments arranged by size:
2579 1434 1098 962 718 111 87 17
Bpu10I CC'TnA_GC
Cuts at: 3291 3291
Size: 7006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2792 3584 5131 5996 6294 2792
Size: 792 1547 865 298 3504
Fragments arranged by size:
3504 1547 865 792 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5669 882
Size: 617 4170 2219
Fragments arranged by size:
4170 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3433 4303 4797 493
Size: 711 2229 870 494 2702
Fragments arranged by size:
2702 2229 870 711 494
BsaBI GATnn'nnATC
Cuts at: 7000 7000
Size: 7006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3458 3911 4010
Size: 53 83 186 936 543 1382 453 99
Cuts at: 4010 4221 4813 5195 5830 275
Size: 211 592 382 635 1451
Fragments arranged by size:
1451 1382 936 635 592 543 453 382
211 186 99 83 53
BsaWI w'CCGG_w
Cuts at: 5439 5837 6111 6258 6669 5439
Size: 398 274 147 411 5776
Fragments arranged by size:
5776 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3226 3256 3966 3996 4235
Size: 30 165 30 1823 30 710 30 239
Cuts at: 4235 4265 1178
Size: 30 3919
Fragments arranged by size:
3919 1823 710 239 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3740 1186
Size: 2554 4452
BseYI C'CCAG_C
Cuts at: 2091 3177 4033 6209 6705 2091
Size: 1086 856 2176 496 2392
Fragments arranged by size:
2392 2176 1086 856 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 2990 3143 3384 3521 3735 3779
Size: 573 768 486 153 241 137 214 44
Cuts at: 3779 3941 4001 4148 1163
Size: 162 60 147 4021
Fragments arranged by size:
4021 768 573 486 241 214 162 153
147 137 60 44
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4456 5217 6245 1540
Size: 275 186 2455 761 1028 2301
Fragments arranged by size:
2455 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 2852 3221 4248 5067 5152 6223 2415
Size: 437 369 1027 819 85 1071 3198
Fragments arranged by size:
3198 1071 1027 819 437 369 85
BsmI GAATG_Cn'
Cuts at: 4517 4610 4517
Size: 93 6913
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2365 2415 2852 3005 3221 3570 3608
Size: 399 575 50 437 153 216 349 38
Cuts at: 3608 3807 3832 3941 4248 4325 5067 5152 6223
Size: 199 25 109 307 77 742 85 1071
Cuts at: 6223 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 575 437 399 349 307
216 199 153 109 85 77 50 38
25
BspEI T'CCGG_A
Cuts at: 6669 6669
Size: 7006
BspHI T'CATG_A
Cuts at: 4895 6625 4895
Size: 1730 5276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4248 4361 844
Size: 3404 113 3489
Fragments arranged by size:
3489 3404 113
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 2878 3590 3977 4893 1394
Size: 429 250 805 712 387 916 3507
Fragments arranged by size:
3507 916 805 712 429 387 250
BsrDI GCAATG_nn'
Cuts at: 65 1248 5501 5675 65
Size: 1183 4253 174 1396
Fragments arranged by size:
4253 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2331 2757 3134 4043 5650 5837
Size: 110 223 298 426 377 909 1607 187
Cuts at: 5837 1700
Size: 2869
Fragments arranged by size:
2869 1607 909 426 377 298 223 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7006
BssHII G'CGCG_C
Cuts at: 1925 4169 1925
Size: 2244 4762
BssSI C'ACGA_G
Cuts at: 2339 3052 3280 5066 6078 2339
Size: 713 228 1786 1012 3267
Fragments arranged by size:
3267 1786 1012 713 228
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3121 3216 3827 3928 794
Size: 2327 95 611 101 3872
Fragments arranged by size:
3872 2327 611 101 95
BstBI TT'CG_AA
Cuts at: 2007 5810 2007
Size: 3803 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7006
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 3492 4033 2701
Size: 791 541 5674
Fragments arranged by size:
5674 791 541
BstYI r'GATC_y
Cuts at: 1972 2451 3339 4336 4703 5088 5105 6557 6643
Size: 479 888 997 367 385 17 1452 86
Cuts at: 6643 6655 7001 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 997 888 479 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2823 3462 3865 4239 513
Size: 552 957 801 639 403 374 3280
Fragments arranged by size:
3280 957 801 639 552 403 374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2965 4105 487
Size: 683 56 1739 1140 3388
Fragments arranged by size:
3388 1739 1140 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 3247 3540 4131 4154 4522 5316 5336 2102
Size: 1145 293 591 23 368 794 20 3772
Fragments arranged by size:
3772 1145 794 591 368 293 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7006
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6971
DraI TTT'AAA
Cuts at: 4310 4310
Size: 7006
DraIII CAC_nnn'GTG
Cuts at: 2344 2344
Size: 7006
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6013 809
Size: 507 4697 1802
Fragments arranged by size:
4697 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3132 3160 3585
Size: 54 1425 50 275 18 1302 28 425
Cuts at: 3585 4045 4116 4134 4453 5342 6971 8
Size: 460 71 18 319 889 1629 43
Fragments arranged by size:
1629 1425 1302 889 460 425 319 275
71 54 50 43 28 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4453 1537
Size: 275 2641 4090
Fragments arranged by size:
4090 2641 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 3644 3671 4287 4936 1353
Size: 1373 918 27 616 649 3423
Fragments arranged by size:
3423 1373 918 649 616 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2378 2831 2977 3058 3614 3794
Size: 1064 138 789 453 146 81 556 180
Cuts at: 3794 3902 4193 5593 5967 6113 387
Size: 108 291 1400 374 146 1280
Fragments arranged by size:
1400 1280 1064 789 556 453 374 291
180 146 146 138 108 81
EcoICRI GAG'CTC
Cuts at: 4246 4246
Size: 7006
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2312 2399 3497 3514 4085 4232
Size: 393 569 111 87 1098 17 571 147
Cuts at: 4232 5069 5666 6453 6721 1239
Size: 837 597 787 268 1524
Fragments arranged by size:
1524 1098 837 787 597 571 569 393
268 147 111 87 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 7006
EcoO109I rG'GnC_Cy
Cuts at: 2287 2320 2361 2362 3481 3772 4108 2287
Size: 33 41 1 1119 291 336 5185
Fragments arranged by size:
5185 1119 336 291 41 33 1
EcoRI G'AATT_C
Cuts at: 4315 4693 4315
Size: 378 6628
FspI TGC'GCA
Cuts at: 5512 5512
Size: 7006
HaeII r_GCGC'y
Cuts at: 1678 2764 3330 3461 3860 4013 4224 6153 1678
Size: 1086 566 131 399 153 211 1929 2531
Fragments arranged by size:
2531 1929 1086 566 399 211 153 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2885 2917 5647 5679 5721
Size: 32 135 32 1313 32 2730 32 42
Cuts at: 5721 5753 1373
Size: 32 2626
Fragments arranged by size:
2730 2626 1313 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3880 4350 4596 669
Size: 988 281 1942 470 246 3079
Fragments arranged by size:
3079 1942 988 470 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7006
HpaI GTT'AAC
Cuts at: 4596 4596
Size: 7006
KasI G'GCGC_C
Cuts at: 3457 4009 4220 3457
Size: 552 211 6243
Fragments arranged by size:
6243 552 211
KpnI G_GTAC'C
Cuts at: 4331 4331
Size: 7006
MfeI C'AATT_G
Cuts at: 4605 4605
Size: 7006
MluI A'CGCG_T
Cuts at: 6882 6882
Size: 7006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3702 6120 6304 1305
Size: 2397 2418 184 2007
Fragments arranged by size:
2418 2397 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4118 10
Size: 54 1425 343 2286 2898
Fragments arranged by size:
2898 2286 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2825 4124 4964 5323 5482 518
Size: 648 1659 1299 840 359 159 2042
Fragments arranged by size:
2042 1659 1299 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2569 2632 2788 3177 3735 3916 4105
Size: 921 42 63 156 389 558 181 189
Cuts at: 4105 4422 5099 6247 6492 1606
Size: 317 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 558 389 317 245
189 181 156 63 42
NaeI GCC'GGC
Cuts at: 3136 3136
Size: 7006
NarI GG'CG_CC
Cuts at: 3458 4010 4221 3458
Size: 552 211 6243
Fragments arranged by size:
6243 552 211
NcoI C'CATG_G
Cuts at: 513 1065 2022 3462 513
Size: 552 957 1440 4057
Fragments arranged by size:
4057 1440 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7006
NgoMIV G'CCGG_C
Cuts at: 3134 3134
Size: 7006
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7006
NotI GC'GGCC_GC
Cuts at: 4453 4453
Size: 7006
NspI r_CATG'y
Cuts at: 1132 1229 2923 4364 5909 1132
Size: 97 1694 1441 1545 2229
Fragments arranged by size:
2229 1694 1545 1441 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7006
PciI A'CATG_T
Cuts at: 5905 5905
Size: 7006
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 1108
Size: 1236 5770
PfoI T'CCnGG_A
Cuts at: 2454 4266 2454
Size: 1812 5194
PmeI GTTT'AAAC
Cuts at: 4310 4310
Size: 7006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 4235 4267 5075 5107 6588 6620 2867
Size: 32 1336 32 808 32 1481 32 3253
Fragments arranged by size:
3253 1481 1336 808 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2287 4108 2287
Size: 1821 5185
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7006
PsiI TTA'TAA
Cuts at: 4576 4576
Size: 7006
PspOMI G'GGCC_C
Cuts at: 2361 2361
Size: 7006
PstI C_TGCA'G
Cuts at: 830 2133 2433 2646 2928 3117 4358 830
Size: 1303 300 213 282 189 1241 3478
Fragments arranged by size:
3478 1303 1241 300 282 213 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7006
PvuII CAG'CTG
Cuts at: 2527 2569 2788 2527
Size: 42 219 6745
Fragments arranged by size:
6745 219 42
SacI G_AGCT'C
Cuts at: 4248 4248
Size: 7006
SalI G'TCGA_C
Cuts at: 1936 4348 1936
Size: 2412 4594
SanDI GG'GwC_CC
Cuts at: 4108 4108
Size: 7006
SapI GCTCTTCn'nnn_
Cuts at: 3671 3671
Size: 7006
SbfI CC_TGCA'GG
Cuts at: 4358 4358
Size: 7006
ScaI AGT'ACT
Cuts at: 5254 6966 5254
Size: 1712 5294
SfcI C'TryA_G
Cuts at: 826 1045 2129 2150 2429 2509 2642 2924 3016
Size: 219 1084 21 279 80 133 282 92
Cuts at: 3016 3113 4354 5489 6170 6361 826
Size: 97 1241 1135 681 191 1471
Fragments arranged by size:
1471 1241 1135 1084 681 282 279 219
191 133 97 92 80 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 3869 4044 3869
Size: 175 6831
SfoI GGC'GCC
Cuts at: 3459 4011 4222 3459
Size: 552 211 6243
Fragments arranged by size:
6243 552 211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7006
SmaI CCC'GGG
Cuts at: 1872 3801 4218 4333 1872
Size: 1929 417 115 4545
Fragments arranged by size:
4545 1929 417 115
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2807 3563 5110 6011 6273 820
Size: 197 926 864 756 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 864 756 262 197
SnaBI TAC'GTA
Cuts at: 493 4303 4797 493
Size: 3810 494 2702
Fragments arranged by size:
3810 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7006
SphI G_CATG'C
Cuts at: 1132 2923 4364 1132
Size: 1791 1441 3774
Fragments arranged by size:
3774 1791 1441
SspI AAT'ATT
Cuts at: 5 52 4930 5
Size: 47 4878 2081
Fragments arranged by size:
4878 2081 47
StuI AGG'CCT
Cuts at: 1517 2042 3413 3767 1517
Size: 525 1371 354 4756
Fragments arranged by size:
4756 1371 525 354
StyI C'CwwG_G
Cuts at: 513 1065 2022 3255 3462 4445 513
Size: 552 957 1233 207 983 3074
Fragments arranged by size:
3074 1233 983 957 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2920 3230 5033 5050 5203 5388 5833
Size: 1255 1340 310 1803 17 153 185 445
Cuts at: 5833 325
Size: 1498
Fragments arranged by size:
1803 1498 1340 1255 445 310 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5252 6964 96
Size: 274 80 33 51 1443 3275 1712 138
Fragments arranged by size:
3275 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2471 2503 3226 3258 3966 3998 6966 6998 2471
Size: 32 723 32 708 32 2968 32 2479
Fragments arranged by size:
2968 2479 723 708 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 1316
Size: 795 6211
XbaI T'CTAG_A
Cuts at: 4342 4342
Size: 7006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3190 3190
Size: 7006
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7006
XmaI C'CCGG_G
Cuts at: 1870 3799 4216 4331 1870
Size: 1929 417 115 4545
Fragments arranged by size:
4545 1929 417 115
XmnI GAAnn'nnTTC
Cuts at: 2384 5135 2384
Size: 2751 4255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4814 276
Size: 53 83 186 936 3280 2468
Fragments arranged by size:
3280 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SexAI SrfI SwaI