Restriction map for
pFN21ASDA1715
(Circular) (Six-base) MAPSORT of: pFN21ASDA1715.seq Check: 4182 from: 1 to: 6013
pFN21ASDA1715 6013 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2989 2989
Size: 6013
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 4076 5460 5728 1632
Size: 1065 1379 1384 268 1917
Fragments arranged by size:
1917 1384 1379 1065 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 2720 3251 3756 5328 2720
Size: 531 505 1572 3405
Fragments arranged by size:
3405 1572 531 505
ApaI G_GGCC'C
Cuts at: 2582 2582
Size: 6013
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2094 2111 2228 2891 3322 3548 3700 5809
Size: 601 17 117 663 431 226 152 2109
Cuts at: 5809 1493
Size: 1697
Fragments arranged by size:
2109 1697 663 601 431 226 152 117
17
AseI AT'TA_AT
Cuts at: 160 1032 2159 2423 4567 160
Size: 872 1127 264 2144 1606
Fragments arranged by size:
2144 1606 1127 872 264
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2772 3338 1870
Size: 73 829 566 4545
Fragments arranged by size:
4545 829 566 73
BamHI G'GATC_C
Cuts at: 2699 3343 3710 2699
Size: 644 367 5002
Fragments arranged by size:
5002 644 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 4789 618
Size: 325 217 627 1547 1455 1842
Fragments arranged by size:
1842 1547 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2582 3332 1391
Size: 1191 750 4072
Fragments arranged by size:
4072 1191 750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2723 928
Size: 971 824 4218
Fragments arranged by size:
4218 971 824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 2277 1548
Size: 729 5284
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2582 4074 5230 1790
Size: 792 1492 1156 2573
Fragments arranged by size:
2573 1492 1156 792
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3744 4702 442
Size: 416 53 83 399 24 2327 958 1753
Fragments arranged by size:
2327 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2305 2505 2646 2724 3273 4673 1239
Size: 1066 200 141 78 549 1400 2579
Fragments arranged by size:
2579 1400 1066 549 200 141 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4676 882
Size: 617 3177 2219
Fragments arranged by size:
3177 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3310 3804 493
Size: 711 2106 494 2702
Fragments arranged by size:
2702 2106 711 494
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4202 4837 275
Size: 53 83 186 936 2287 382 635 1451
Fragments arranged by size:
2287 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2443 4446 4844 5118 5265 5676 2443
Size: 2003 398 274 147 411 2780
Fragments arranged by size:
2780 2003 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2478 2508 2644 2674 2751
Size: 30 165 30 1075 30 136 30 77
Cuts at: 2751 2781 1178
Size: 30 4410
Fragments arranged by size:
4410 1075 165 136 77 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2574 3206 1186
Size: 1388 632 3993
Fragments arranged by size:
3993 1388 632
BseYI C'CCAG_C
Cuts at: 5216 5712 5216
Size: 496 5517
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4074 4159 5230 4074
Size: 85 1071 4857
Fragments arranged by size:
4857 1071 85
BsmI GAATG_Cn'
Cuts at: 3524 3617 3524
Size: 93 5920
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2582 3332 4074 4159 5230 1391
Size: 399 792 750 742 85 1071 2174
Fragments arranged by size:
2174 1071 792 750 742 399 85
BspEI T'CCGG_A
Cuts at: 2443 5676 2443
Size: 3233 2780
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2989 3368 844
Size: 2145 379 3489
Fragments arranged by size:
3489 2145 379
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2901 4508 4682 65
Size: 1183 1653 1607 174 1396
Fragments arranged by size:
1653 1607 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2243 794
Size: 1449 4564
BstBI TT'CG_AA
Cuts at: 2007 4817 2007
Size: 2810 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2699 3343 3710 4095 4112 5564 5650 5662
Size: 727 644 367 385 17 1452 86 12
Cuts at: 5662 6008 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 727 644 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2958 2958
Size: 6013
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4504
Fragments arranged by size:
4504 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 2861 3529 4323 4343 2861
Size: 668 794 20 4531
Fragments arranged by size:
4531 794 668 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 2358 3317 2358
Size: 959 5054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978
Size: 54 1425 50 275 18 1630 889 1629
Cuts at: 5978 8
Size: 43
Fragments arranged by size:
1630 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2987 3943 1353
Size: 1634 956 3423
Fragments arranged by size:
3423 1634 956
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2505 2646 2697 2724 3273 4076
Size: 393 673 200 141 51 27 549 803
Cuts at: 4076 4673 5460 5728 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 803 787 673 597 549 393 268
200 141 51 27
EcoO109I rG'GnC_Cy
Cuts at: 2578 2579 2578
Size: 1 6012
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2432 2462 2464
Size: 32 135 32 773 32 55 30 2
Cuts at: 2464 2494 2644 2676 2751 2783 4654 4686 4728
Size: 30 150 32 75 32 1871 32 42
Cuts at: 4728 4760 1373
Size: 32 2626
Fragments arranged by size:
2626 1871 773 150 135 75 55 42
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2131 2579 5127 5311 1305
Size: 826 448 2548 184 2007
Fragments arranged by size:
2548 2007 826 448 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2845 3971 4330 4489 518
Size: 648 1679 1126 359 159 2042
Fragments arranged by size:
2042 1679 1126 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2492 3429 4106 5254 5499 1606
Size: 886 937 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 886 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4504
Fragments arranged by size:
4504 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2196 3371 4916 1132
Size: 97 967 1175 1545 2229
Fragments arranged by size:
2229 1545 1175 967 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2483 1108
Size: 1375 4638
PfoI T'CCnGG_A
Cuts at: 2605 3250 2605
Size: 645 5368
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2521 2553 4082 4114 5595 5627 2521
Size: 32 1529 32 1481 32 2907
Fragments arranged by size:
2907 1529 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 2578 2578
Size: 6013
PstI C_TGCA'G
Cuts at: 830 2536 3365 830
Size: 1706 829 3478
Fragments arranged by size:
3478 1706 829
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
PvuII CAG'CTG
Cuts at: 2492 2492
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 2064 2532 3138 3361 4496 5177 5368
Size: 219 1019 468 606 223 1135 681 191
Cuts at: 5368 826
Size: 1471
Fragments arranged by size:
1471 1135 1019 681 606 468 223 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4117 5018 5280 820
Size: 197 926 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3310 3804 493
Size: 2817 494 2702
Fragments arranged by size:
2817 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 2807 3937 5
Size: 47 2755 1130 2081
Fragments arranged by size:
2755 2081 1130 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2022 3452 513
Size: 552 957 1430 3074
Fragments arranged by size:
3074 1430 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 4040 4057 4210 4395 4840 325
Size: 1255 435 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 435 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4259 5971 96
Size: 274 80 33 51 1443 2282 1712 138
Fragments arranged by size:
2282 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 2074 3349 2074
Size: 1275 4738
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2280 2280
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2548 4142 2548
Size: 1594 4419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI