Restriction map for
pFN21ASDA1710
(Circular) (Six-base) MAPSORT of: pFN21ASDA1710.seq Check: 2027 from: 1 to: 6139
pFN21ASDA1710 6139 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3482 1937
Size: 1545 4594
Acc65I G'GTAC_C
Cuts at: 3460 3460
Size: 6139
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2446 2530 2782 2866 2950 3118 4202 5586
Size: 814 84 252 84 84 168 1084 1384
Cuts at: 5586 5854 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1084 814 268 252 168 84
84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 5038 6015 1203
Size: 3835 977 1327
Fragments arranged by size:
3835 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 2757 2841 1166
Size: 1591 84 4464
Fragments arranged by size:
4464 1591 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 2390 3882 5454 2390
Size: 1492 1572 3075
Fragments arranged by size:
3075 1572 1492
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3046 3168 3448 3674 3826 5935 1493
Size: 1553 122 280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1553 280 226 152 122
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3464 1870
Size: 73 151 1370 4545
Fragments arranged by size:
4545 1370 151 73
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 3460 4915 618
Size: 325 217 627 269 1404 1455 1842
Fragments arranged by size:
1842 1455 1404 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2810 3004 3146 3314 3398 3458 1391
Size: 1419 194 142 168 84 60 4072
Fragments arranged by size:
4072 1419 194 168 142 84 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2501 928
Size: 971 602 4566
Fragments arranged by size:
4566 971 602
BbvCI CC'TCA_GC
Cuts at: 2280 2280
Size: 6139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4750 136
Size: 107 122 71 631 605 3078 1525
Fragments arranged by size:
3078 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2469 2891 3232 6134 2469
Size: 422 341 2902 2474
Fragments arranged by size:
2902 2474 422 341
BlpI GC'TnA_GC
Cuts at: 2820 3397 3556 2820
Size: 577 159 5403
Fragments arranged by size:
5403 577 159
Bme1580I G_kGCm'C
Cuts at: 1790 4200 5356 1790
Size: 2410 1156 2573
Fragments arranged by size:
2573 2410 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2598 3870 4828
Size: 416 53 83 399 24 1181 1272 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1753 1272 1181 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3417 3417
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2526 2694 3114 3198 4799 1239
Size: 1287 168 420 84 1601 2579
Fragments arranged by size:
2579 1601 1287 420 168 84
Bpu10I CC'TnA_GC
Cuts at: 2280 2280
Size: 6139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2101 4802 882
Size: 617 602 2701 2219
Fragments arranged by size:
2701 2219 617 602
BsaAI yAC'GTr
Cuts at: 493 1204 3436 3930 493
Size: 711 2232 494 2702
Fragments arranged by size:
2702 2232 711 494
BsaBI GATnn'nnATC
Cuts at: 6133 6133
Size: 6139
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3946 4328 4963 275
Size: 53 83 186 936 2413 382 635 1451
Fragments arranged by size:
2413 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2795 1186
Size: 1609 4530
BseYI C'CCAG_C
Cuts at: 2045 5342 5838 2045
Size: 3297 496 2346
Fragments arranged by size:
3297 2346 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2106 3305 1163
Size: 573 370 1199 3997
Fragments arranged by size:
3997 1199 573 370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3589 4350 5378 1540
Size: 275 186 1588 761 1028 2301
Fragments arranged by size:
2301 1588 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2810 3146 3314 3398 4200 4285 5356 2810
Size: 336 168 84 802 85 1071 3593
Fragments arranged by size:
3593 1071 802 336 168 85 84
BsmI GAATG_Cn'
Cuts at: 2281 3116 3650 3743 2281
Size: 835 534 93 4677
Fragments arranged by size:
4677 835 534 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2726 2726
Size: 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2810 3004 3146 3314 3398 3458 4200
Size: 399 1020 194 142 168 84 60 742
Cuts at: 4200 4285 5356 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1020 742 399 194 168 142
85 84 60
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2275 3494 844
Size: 1431 1219 3489
Fragments arranged by size:
3489 1431 1219
BsrBI CCG'CTC
Cuts at: 1394 1823 4026 1394
Size: 429 2203 3507
Fragments arranged by size:
3507 2203 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3121 4634 4808 65
Size: 1183 1873 1513 174 1396
Fragments arranged by size:
1873 1513 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4783 4970 1700
Size: 110 2973 187 2869
Fragments arranged by size:
2973 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2239 96
Size: 2143 3996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6139
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 2007 4943 2007
Size: 2936 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstYI r'GATC_y
Cuts at: 1972 2469 2891 3232 3469 3836 4221 4238 5690
Size: 497 422 341 237 367 385 17 1452
Cuts at: 5690 5776 5788 6134 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 497 422 385 367 346 341
237 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4630
Fragments arranged by size:
4630 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2978 487
Size: 683 56 1752 3648
Fragments arranged by size:
3648 1752 683 56
BtsI GCAGTG_nn'
Cuts at: 3293 3655 4449 4469 3293
Size: 362 794 20 4963
Fragments arranged by size:
4963 794 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3103 3138 529
Size: 35 2539 35 3530
Fragments arranged by size:
3530 2539 35 35
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2872 3586
Size: 54 1425 50 275 18 249 793 714
Cuts at: 3586 4475 6104 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 793 714 275 249 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3586 1537
Size: 275 1774 4090
Fragments arranged by size:
4090 1774 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2295 2352 3374 4069 1353
Size: 942 57 1022 695 3423
Fragments arranged by size:
3423 1022 942 695 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4726 5100 5246 387
Size: 1064 138 3137 374 146 1280
Fragments arranged by size:
3137 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2808 3144 3312 3396 2808
Size: 336 168 84 5551
Fragments arranged by size:
5551 336 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2446 2526 2530 2694 2782 2866 2950
Size: 393 814 80 4 164 88 84 84
Cuts at: 2950 3114 3118 3198 4202 4799 5586 5854 1239
Size: 164 4 80 1004 597 787 268 1524
Fragments arranged by size:
1524 1004 814 787 597 393 268 164
164 88 84 84 80 80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2049 2131 2748 2832 2934 2049
Size: 82 617 84 102 5254
Fragments arranged by size:
5254 617 102 84 82
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 5286 1678
Size: 3608 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2641 2673 2881 2883 2913
Size: 32 135 32 1069 32 208 2 30
Cuts at: 2913 2915 3133 3165 4780 4812 4854 4886 1373
Size: 2 218 32 1615 32 42 32 2626
Fragments arranged by size:
2626 1615 1069 218 208 135 42 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3483 3729 669
Size: 988 281 1545 246 3079
Fragments arranged by size:
3079 1545 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2794 3130 3214 748
Size: 2046 336 84 3673
Fragments arranged by size:
3673 2046 336 84
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KpnI G_GTAC'C
Cuts at: 3464 3464
Size: 6139
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2404 2761 3003 3339 5253 5437 1305
Size: 1099 357 242 336 1914 184 2007
Fragments arranged by size:
2007 1914 1099 357 336 242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 10
Size: 54 1425 343 249 4068
Fragments arranged by size:
4068 1425 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 2841 3109 4097 4456 4615 518
Size: 648 1591 84 268 988 359 159 2042
Fragments arranged by size:
2042 1591 988 648 359 268 159 84
MspA1I CmG'CkG
Cuts at: 1606 3555 4232 5380 5625 1606
Size: 1949 677 1148 245 2120
Fragments arranged by size:
2120 1949 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4630
Fragments arranged by size:
4630 957 552
NdeI CA'TA_TG
Cuts at: 387 3106 387
Size: 2719 3420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 3586 3586
Size: 6139
NspI r_CATG'y
Cuts at: 1132 1229 3497 5042 1132
Size: 97 2268 1545 2229
Fragments arranged by size:
2268 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6139
PciI A'CATG_T
Cuts at: 5038 5038
Size: 6139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6139
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2049 2131 2748 2832 2049
Size: 82 617 84 5356
Fragments arranged by size:
5356 617 84 82
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PstI C_TGCA'G
Cuts at: 830 3491 830
Size: 2661 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
SacI G_AGCT'C
Cuts at: 2810 3146 3314 3398 2810
Size: 336 168 84 5551
Fragments arranged by size:
5551 336 168 84
SalI G'TCGA_C
Cuts at: 1936 3481 1936
Size: 1545 4594
SanDI GG'GwC_CC
Cuts at: 2049 2049
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 3491 3491
Size: 6139
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 3487 4622 5303 5494 826
Size: 219 2442 1135 681 191 1471
Fragments arranged by size:
2442 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 2096 3466 1872
Size: 224 1370 4545
Fragments arranged by size:
4545 1370 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4243 5144 5406 820
Size: 197 926 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3436 3930 493
Size: 2943 494 2702
Fragments arranged by size:
2943 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 2599 1517
Size: 942 140 5057
Fragments arranged by size:
5057 942 140
StyI C'CwwG_G
Cuts at: 513 1065 2022 3578 513
Size: 552 957 1556 3074
Fragments arranged by size:
3074 1556 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 4166 4183 4336 4521 4966 325
Size: 1255 1740 846 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 846 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2239 4385 6097
Size: 274 80 33 51 1443 262 2146 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
2146 1712 1443 274 262 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2740 2772 6099 6131 2740
Size: 32 3327 32 2748
Fragments arranged by size:
3327 2748 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6139
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2038
Size: 6139
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6139
XmaI C'CCGG_G
Cuts at: 1870 2094 3464 1870
Size: 224 1370 4545
Fragments arranged by size:
4545 1370 224
XmnI GAAnn'nnTTC
Cuts at: 2638 2795 2963 3131 3299 3383 4268 2638
Size: 157 168 168 168 84 885 4509
Fragments arranged by size:
4509 885 168 168 168 157 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI