Restriction map for
pFN21ASDA1706
(Circular) (Six-base) MAPSORT of: pFN21ASDA1706.seq Check: 2955 from: 1 to: 6436
pFN21ASDA1706 6436 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3779 1937
Size: 1842 4594
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 4499 5883 6151 1632
Size: 463 2404 1384 268 1917
Fragments arranged by size:
2404 1917 1384 463 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6239
AflIII A'CryG_T
Cuts at: 1203 2192 2428 5335 6312 1203
Size: 989 236 2907 977 1327
Fragments arranged by size:
2907 1327 989 977 236
AgeI A'CCGG_T
Cuts at: 3294 5267 3294
Size: 1973 4463
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 2411 1166
Size: 1245 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 4179 5751 4179
Size: 1572 4864
ApaI G_GGCC'C
Cuts at: 2532 2736 3009 3173 2532
Size: 204 273 164 5795
Fragments arranged by size:
5795 273 204 164
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 3745 3971 4123 6232 1493
Size: 2252 226 152 2109 1697
Fragments arranged by size:
2252 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2677 2858 3009 3185 3761 1870
Size: 73 734 181 151 176 576 4545
Fragments arranged by size:
4545 734 576 181 176 151 73
BamHI G'GATC_C
Cuts at: 2592 3766 4133 2592
Size: 1174 367 4895
Fragments arranged by size:
4895 1174 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2598 3757 5212 618
Size: 325 217 627 495 316 1159 1455 1842
Fragments arranged by size:
1842 1455 1159 627 495 325 316 217
BanII G_rGCy'C
Cuts at: 1391 2532 2684 2736 3009 3140 3173 3755 1391
Size: 1141 152 52 273 131 33 582 4072
Fragments arranged by size:
4072 1141 582 273 152 131 52 33
BbeI G_GCGC'C
Cuts at: 2286 2286
Size: 6436
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5465
BbvCI CC'TCA_GC
Cuts at: 2498 2804 2498
Size: 306 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4627 4661 1903
Size: 34 2690 34 3678
Fragments arranged by size:
3678 2690 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 2731 5047
Size: 107 122 71 631 605 781 278 2316
Cuts at: 5047 136
Size: 1525
Fragments arranged by size:
2316 1525 781 631 605 278 122 107
71
BglII A'GATC_T
Cuts at: 2313 6431 2313
Size: 4118 2318
BlpI GC'TnA_GC
Cuts at: 2338 3853 2338
Size: 1515 4921
Bme1580I G_kGCm'C
Cuts at: 1790 2418 2532 2736 3009 3156 3173 4497 5653
Size: 628 114 204 273 147 17 1324 1156
Cuts at: 5653 1790
Size: 2573
Fragments arranged by size:
2573 1324 1156 628 273 204 147 114
17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 3547 3614
Size: 416 53 83 399 24 932 1198 67
Cuts at: 3614 4167 5125 442
Size: 553 958 1753
Fragments arranged by size:
1753 1198 958 932 553 416 399 83
67 53 24
BmtI G_CTAG'C
Cuts at: 1056 2381 3629 1056
Size: 1325 1248 3863
Fragments arranged by size:
3863 1325 1248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3575 3061
Size: 514 5922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 3041 3673 5096 1239
Size: 1076 726 632 1423 2579
Fragments arranged by size:
2579 1423 1076 726 632
Bpu10I CC'TnA_GC
Cuts at: 2498 2693 2804 2498
Size: 195 111 6130
Fragments arranged by size:
6130 195 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3456 4561 5426 5724 3456
Size: 1105 865 298 4168
Fragments arranged by size:
4168 1105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5099 882
Size: 617 3600 2219
Fragments arranged by size:
3600 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2429 3733 4227 493
Size: 711 1225 1304 494 2702
Fragments arranged by size:
2702 1304 1225 711 494
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 3666 4243 4625
Size: 53 83 186 936 750 1383 577 382
Cuts at: 4625 5260 275
Size: 635 1451
Fragments arranged by size:
1451 1383 936 750 635 577 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3294 3577 4869 5267 5541 5688 6099 3294
Size: 283 1292 398 274 147 411 3631
Fragments arranged by size:
3631 1292 411 398 283 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6211
Fragments arranged by size:
6211 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2180 1186
Size: 991 3 5442
Fragments arranged by size:
5442 991 3
BseYI C'CCAG_C
Cuts at: 2457 3068 3173 3269 5639 6135 2457
Size: 611 105 96 2370 496 2758
Fragments arranged by size:
2758 2370 611 496 105 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2941 1163
Size: 573 1205 4658
Fragments arranged by size:
4658 1205 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2759 3886 4647 5675 1540
Size: 275 186 758 1127 761 1028 2301
Fragments arranged by size:
2301 1127 1028 761 758 275 186
BsiHKAI G_wGCw'C
Cuts at: 2033 3140 4497 4582 5653 2033
Size: 1107 1357 85 1071 2816
Fragments arranged by size:
2816 1357 1107 1071 85
BsmI GAATG_Cn'
Cuts at: 3947 4040 3947
Size: 93 6343
BsmBI CGTCTCn'nnnn_
Cuts at: 3111 3111
Size: 6436
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2418 2532 2684 2736 3009 3140
Size: 399 243 385 114 152 52 273 131
Cuts at: 3140 3156 3173 3755 4497 4582 5653 1391
Size: 16 17 582 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 399 385 273 243
152 131 114 85 52 17 16
BspEI T'CCGG_A
Cuts at: 3577 6099 3577
Size: 2522 3914
BspHI T'CATG_A
Cuts at: 2463 4325 6055 2463
Size: 1862 1730 2844
Fragments arranged by size:
2844 1862 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 2382 2727 3791 844
Size: 1446 92 345 1064 3489
Fragments arranged by size:
3489 1446 1064 345 92
BsrBI CCG'CTC
Cuts at: 1394 1823 2049 2137 4323 1394
Size: 429 226 88 2186 3507
Fragments arranged by size:
3507 2186 429 226 88
BsrDI GCAATG_nn'
Cuts at: 65 1248 3394 4931 5105 65
Size: 1183 2146 1537 174 1396
Fragments arranged by size:
2146 1537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3101 3294 5080 5267 1700
Size: 110 1291 193 1786 187 2869
Fragments arranged by size:
2869 1786 1291 193 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 3722 4496 5508 3722
Size: 774 1012 4650
Fragments arranged by size:
4650 1012 774
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2926 794
Size: 2132 4304
BstBI TT'CG_AA
Cuts at: 2007 5240 2007
Size: 3233 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2293
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 2313 2592 3766 4133 4518 4535 5987 6073
Size: 341 279 1174 367 385 17 1452 86
Cuts at: 6073 6085 6431 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1174 385 367 346 341 279
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3404 3404
Size: 6436
BtgI C'CryG_G
Cuts at: 513 1065 2022 2157 2199 2763 2838 513
Size: 552 957 135 42 564 75 4111
Fragments arranged by size:
4111 957 564 552 135 75 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5697
Fragments arranged by size:
5697 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 3613 3952 4746 4766 2151
Size: 1462 339 794 20 3821
Fragments arranged by size:
3821 1462 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6401
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DraIII CAC_nnn'GTG
Cuts at: 2605 2605
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 2756 2811
Size: 54 1425 50 275 18 573 353 55
Cuts at: 2811 3289 3370 3883 4772 6401 8
Size: 478 81 513 889 1629 43
Fragments arranged by size:
1629 1425 889 573 513 478 353 275
81 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2756 3883 1537
Size: 275 944 1127 4090
Fragments arranged by size:
4090 1127 944 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4366 1353
Size: 3013 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2504 5023 5397 5543 387
Size: 1064 138 915 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 915 374 146 138
EcoICRI GAG'CTC
Cuts at: 3138 3138
Size: 6436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2315 3041 3673 4499 5096 5883
Size: 393 463 220 726 632 826 597 787
Cuts at: 5883 6151 1239
Size: 268 1524
Fragments arranged by size:
1524 826 787 726 632 597 463 393
268 220
EcoO109I rG'GnC_Cy
Cuts at: 2062 2257 2371 2529 2548 2625 2710 2732 3005
Size: 195 114 158 19 77 85 22 273
Cuts at: 3005 3006 3170 2062
Size: 1 164 5328
Fragments arranged by size:
5328 273 195 164 158 114 85 77
22 19 1
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
EcoRV GAT'ATC
Cuts at: 3400 3400
Size: 6436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2945 2945
Size: 6436
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 2286 5583 1678
Size: 608 3297 2531
Fragments arranged by size:
3297 2531 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 3250 3282 3572
Size: 32 135 32 645 32 1001 32 290
Cuts at: 3572 3604 5077 5109 5151 5183 1373
Size: 32 1473 32 42 32 2626
Fragments arranged by size:
2626 1473 1001 645 290 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 3226 3780 4026 669
Size: 988 281 496 792 554 246 3079
Fragments arranged by size:
3079 988 792 554 496 281 246
HindIII A'AGCT_T
Cuts at: 748 2906 3511 748
Size: 2158 605 3673
Fragments arranged by size:
3673 2158 605
HpaI GTT'AAC
Cuts at: 2434 3226 4026 2434
Size: 792 800 4844
Fragments arranged by size:
4844 800 792
KasI G'GCGC_C
Cuts at: 2282 2282
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 2192 6312 2192
Size: 4120 2316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5550 5734 1305
Size: 4245 184 2007
Fragments arranged by size:
4245 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2405 2813 3291 3372 10
Size: 54 1425 343 573 408 478 81 3074
Fragments arranged by size:
3074 1425 573 478 408 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2411 2468 3285 4394 4753 4912
Size: 648 957 288 57 817 1109 359 159
Cuts at: 4912 518
Size: 2042
Fragments arranged by size:
2042 1109 957 817 648 359 288 159
57
MspA1I CmG'CkG
Cuts at: 1606 2411 3684 3852 4529 5677 5922 1606
Size: 805 1273 168 677 1148 245 2120
Fragments arranged by size:
2120 1273 1148 805 677 245 168
NaeI GCC'GGC
Cuts at: 3103 3103
Size: 6436
NarI GG'CG_CC
Cuts at: 2283 2283
Size: 6436
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4927
Fragments arranged by size:
4927 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NgoMIV G'CCGG_C
Cuts at: 3101 3101
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 2377 3625 1052
Size: 1325 1248 3863
Fragments arranged by size:
3863 1325 1248
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 3794 5339 1132
Size: 97 2565 1545 2229
Fragments arranged by size:
2565 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2035 2058 1348
Size: 687 23 5726
Fragments arranged by size:
5726 687 23
PciI A'CATG_T
Cuts at: 5335 5335
Size: 6436
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3298 1108
Size: 2190 4246
PfoI T'CCnGG_A
Cuts at: 2583 2889 2583
Size: 306 6130
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PmlI CAC'GTG
Cuts at: 2429 2429
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2257 2371 2548 2710 2062
Size: 195 114 177 162 5788
Fragments arranged by size:
5788 195 177 162 114
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PspOMI G'GGCC_C
Cuts at: 2528 2732 3005 3169 2528
Size: 204 273 164 5795
Fragments arranged by size:
5795 273 204 164
PstI C_TGCA'G
Cuts at: 830 2703 3788 830
Size: 1873 1085 3478
Fragments arranged by size:
3478 1873 1085
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
PvuII CAG'CTG
Cuts at: 2411 2411
Size: 6436
SacI G_AGCT'C
Cuts at: 3140 3140
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SanDI GG'GwC_CC
Cuts at: 2062 2062
Size: 6436
SbfI CC_TGCA'GG
Cuts at: 3788 3788
Size: 6436
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SexAI A'CCwGG_T
Cuts at: 2601 2601
Size: 6436
SfcI C'TryA_G
Cuts at: 826 1045 2699 3320 3784 4919 5600 5791 826
Size: 219 1654 621 464 1135 681 191 1471
Fragments arranged by size:
1654 1471 1135 681 621 464 219 191
SfoI GGC'GCC
Cuts at: 2284 2284
Size: 6436
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 2860 3011 3763 1872
Size: 988 151 752 4545
Fragments arranged by size:
4545 988 752 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3471 4540 5441 5703 820
Size: 197 926 1528 1069 901 262 1553
Fragments arranged by size:
1553 1528 1069 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3733 4227 493
Size: 3240 494 2702
Fragments arranged by size:
3240 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 2478 2771 2797 2942 3558 1517
Size: 961 293 26 145 616 4395
Fragments arranged by size:
4395 961 616 293 145 26
StyI C'CwwG_G
Cuts at: 513 1065 2022 2943 3373 3569 3693 3875 513
Size: 552 957 921 430 196 124 182 3074
Fragments arranged by size:
3074 957 921 552 430 196 182 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 2753 4463 4480 4633 4818 5263
Size: 1255 1071 102 1710 17 153 185 445
Cuts at: 5263 325
Size: 1498
Fragments arranged by size:
1710 1498 1255 1071 445 185 153 102
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4682 6394 96
Size: 274 80 33 51 1443 2705 1712 138
Fragments arranged by size:
2705 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6396 6428 6396
Size: 32 6404
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3065 3065
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 2858 3009 3761 1870
Size: 988 151 752 4545
Fragments arranged by size:
4545 988 752 151
XmnI GAAnn'nnTTC
Cuts at: 3524 4565 3524
Size: 1041 5395
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I EcoNI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SacII SapI SfiI SrfI SwaI