Restriction map for
pFN21ASDA1704
(Circular) (Six-base) MAPSORT of: pFN21ASDA1704.seq Check: 5620 from: 1 to: 5749
pFN21ASDA1704 5749 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2235 2910 3812 5196 5464 1632
Size: 603 675 902 1384 268 1917
Fragments arranged by size:
1917 1384 902 675 603 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 3492 5064 3492
Size: 1572 4177
ApaI G_GGCC'C
Cuts at: 2527 2527
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 3074 1870
Size: 73 158 973 4545
Fragments arranged by size:
4545 973 158 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3070 4525 618
Size: 325 217 627 1283 1455 1842
Fragments arranged by size:
1842 1455 1283 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2108 2527 3068 1391
Size: 717 419 541 4072
Fragments arranged by size:
4072 717 541 419
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 2898 928
Size: 971 282 717 3779
Fragments arranged by size:
3779 971 717 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2938 5744 2938
Size: 2806 2943
BlpI GC'TnA_GC
Cuts at: 2805 3166 2805
Size: 361 5388
Bme1580I G_kGCm'C
Cuts at: 1790 2527 3810 4966 1790
Size: 737 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 737
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2595 2685 2694 4409 1239
Size: 1356 90 9 1715 2579
Fragments arranged by size:
2579 1715 1356 90 9
Bpu10I CC'TnA_GC
Cuts at: 2724 2724
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3874 4739 5037 2927
Size: 947 865 298 3639
Fragments arranged by size:
3639 947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2025 4412 882
Size: 617 526 2387 2219
Fragments arranged by size:
2387 2219 617 526
BsaAI yAC'GTr
Cuts at: 493 1204 3046 3540 493
Size: 711 1842 494 2702
Fragments arranged by size:
2702 1842 711 494
BsaBI GATnn'nnATC
Cuts at: 2842 2937 5743 2842
Size: 95 2806 2848
Fragments arranged by size:
2848 2806 95
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2683 2713 1178
Size: 30 165 30 1280 30 4214
Fragments arranged by size:
4214 1280 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2328 1186
Size: 1142 4607
BseYI C'CCAG_C
Cuts at: 4952 5448 4952
Size: 496 5253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2527 3068 3810 3895 4966 1391
Size: 399 318 419 541 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 541 419 399 318 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2800 3104 844
Size: 1956 304 3489
Fragments arranged by size:
3489 1956 304
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3636 1394
Size: 429 272 1541 3507
Fragments arranged by size:
3507 1541 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 2363 4244 4418 65
Size: 1183 1115 1881 174 1396
Fragments arranged by size:
1881 1396 1183 1115 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 4393 4580 1700
Size: 110 234 2349 187 2869
Fragments arranged by size:
2869 2349 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 2007 4553 2007
Size: 2546 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2938 3079 3446 3831 3848 5300 5386 5398
Size: 966 141 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 966 385 367 346 141 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2071 513
Size: 552 957 49 4191
Fragments arranged by size:
4191 957 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2184 487
Size: 683 56 958 4052
Fragments arranged by size:
4052 958 683 56
BtsI GCAGTG_nn'
Cuts at: 2449 3265 4059 4079 2449
Size: 816 794 20 4119
Fragments arranged by size:
4119 816 794 20
ClaI AT'CG_AT
Cuts at: 1554 2680 1554
Size: 1126 4623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 2320 3053 2320
Size: 733 5016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714
Size: 54 1425 50 275 18 1366 889 1629
Cuts at: 5714 8
Size: 43
Fragments arranged by size:
1629 1425 1366 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 2650 2782 3679 1353
Size: 732 565 132 897 3423
Fragments arranged by size:
3423 897 732 565 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2595 2685 2694 2910 3812 4409
Size: 393 603 360 90 9 216 902 597
Cuts at: 4409 5196 5464 1239
Size: 787 268 1524
Fragments arranged by size:
1524 902 787 603 597 393 360 268
216 90 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2112 2478 2112
Size: 366 5383
EcoO109I rG'GnC_Cy
Cuts at: 2098 2357 2523 2098
Size: 259 166 5324
Fragments arranged by size:
5324 259 166
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 4896 1678
Size: 3218 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2242 2274 2552
Size: 32 135 32 450 32 188 32 278
Cuts at: 2552 2584 2654 2683 2686 2715 2798 2830 4390
Size: 32 70 29 3 29 83 32 1560
Cuts at: 4390 4422 4464 4496 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1560 450 278 188 135 83 70
42 32 32 32 32 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2491 3093 3339 669
Size: 988 281 553 602 246 3079
Fragments arranged by size:
3079 988 602 553 281 246
HindIII A'AGCT_T
Cuts at: 748 2903 748
Size: 2155 3594
HpaI GTT'AAC
Cuts at: 2491 3339 2491
Size: 848 4901
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2258 4863 5047 1305
Size: 953 2605 184 2007
Fragments arranged by size:
2605 2007 953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3707 4066 4225 518
Size: 648 2541 359 159 2042
Fragments arranged by size:
2541 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2073 2081 2705 3165 3842 4990 5235 1606
Size: 467 8 624 460 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 624 467 460 245 8
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4240
Fragments arranged by size:
4240 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 3107 4652 1132
Size: 97 1878 1545 2229
Fragments arranged by size:
2229 1878 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PfoI T'CCnGG_A
Cuts at: 2311 2419 2311
Size: 108 5641
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2357 2098
Size: 259 5490
PshAI GACnn'nnGTC
Cuts at: 1613 2229 2628 1613
Size: 616 399 4734
Fragments arranged by size:
4734 616 399
PsiI TTA'TAA
Cuts at: 2139 3319 2139
Size: 1180 4569
PspOMI G'GGCC_C
Cuts at: 2523 2523
Size: 5749
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2705 2705
Size: 5749
RsrII CG'GwC_CG
Cuts at: 2042 2042
Size: 5749
SacII CC_GC'GG
Cuts at: 2074 2074
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SanDI GG'GwC_CC
Cuts at: 2098 2098
Size: 5749
SapI GCTCTTCn'nnn_
Cuts at: 2085 2650 2085
Size: 565 5184
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SexAI A'CCwGG_T
Cuts at: 2371 2371
Size: 5749
SfcI C'TryA_G
Cuts at: 826 1045 3097 4232 4913 5104 826
Size: 219 2052 1135 681 191 1471
Fragments arranged by size:
2052 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2942 3853 4754 5016 820
Size: 197 926 999 911 901 262 1553
Fragments arranged by size:
1553 999 926 911 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3046 3540 493
Size: 2553 494 2702
Fragments arranged by size:
2702 2553 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2022 2342 2639 3188 513
Size: 552 957 320 297 549 3074
Fragments arranged by size:
3074 957 552 549 320 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 2993 3085 2993
Size: 92 5657
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI
SfiI SfoI SrfI SwaI XcmI