Restriction map for
pFN21ASDA1627
(Circular) (Six-base) MAPSORT of: pFN21ASDA1627.seq Check: 2511 from: 1 to: 6097
pFN21ASDA1627 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2394 3440 1937
Size: 457 1046 4594
Fragments arranged by size:
4594 1046 457
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2342 820
Size: 197 1325 4575
Fragments arranged by size:
4575 1325 197
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 3372 1166
Size: 2206 3891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2296 2416 2576 3009 3406 3632 3784 5893
Size: 803 120 160 433 397 226 152 2109
Cuts at: 5893 1493
Size: 1697
Fragments arranged by size:
2109 1697 803 433 397 226 160 152
120
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 3422 1870
Size: 73 1479 4545
Fragments arranged by size:
4545 1479 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2201 2234 2201
Size: 33 6064
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3418 4873 618
Size: 325 217 627 330 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 330 325 217
BanII G_rGCy'C
Cuts at: 1391 2478 3259 3416 1391
Size: 1087 781 157 4072
Fragments arranged by size:
4072 1087 781 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2840 3052 928
Size: 971 351 590 212 3973
Fragments arranged by size:
3973 971 590 351 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BfrBI ATG'CAT
Cuts at: 3146 3146
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3314 6092 3314
Size: 2778 3319
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 4158 5314 1790
Size: 2368 1156 2573
Fragments arranged by size:
2573 2368 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4757 1239
Size: 3518 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3394 3888 493
Size: 711 2190 494 2702
Fragments arranged by size:
2702 2190 711 494
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3049 3904 4286 4921
Size: 53 83 186 936 1516 855 382 635
Cuts at: 4921 275
Size: 1451
Fragments arranged by size:
1516 1451 936 855 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2182 2212 1178
Size: 30 165 30 779 30 5063
Fragments arranged by size:
5063 779 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2508 3213 3261 3342 3375 1186
Size: 880 442 705 48 81 33 3908
Fragments arranged by size:
3908 880 705 442 81 48 33
BseYI C'CCAG_C
Cuts at: 5300 5796 5300
Size: 496 5601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 3163 3259 3367 4158 4243 5314 2478
Size: 685 96 108 791 85 1071 3261
Fragments arranged by size:
3261 1071 791 685 108 96 85
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2478 3163 3259 3367 3416 4158 4243
Size: 399 688 685 96 108 49 742 85
Cuts at: 4243 5314 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 688 685 399 108 96
85 49
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 3984 1394
Size: 429 230 1931 3507
Fragments arranged by size:
3507 1931 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4741 4928 1700
Size: 110 2931 187 2869
Fragments arranged by size:
2931 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3185 96
Size: 3089 3008
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 2007 4901 2007
Size: 2894 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2043 2848 3314 3427 3794 4179 4196 5648
Size: 71 805 466 113 367 385 17 1452
Cuts at: 5648 5734 5746 6092 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 805 466 385 367 346 113
86 71 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3295 3355 513
Size: 552 957 1273 60 3255
Fragments arranged by size:
3255 1273 957 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 2634 2881 3613 4407 4427 2634
Size: 247 732 794 20 4304
Fragments arranged by size:
4304 794 732 247 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3292 3544 4433
Size: 54 1425 50 275 18 1462 252 889
Cuts at: 4433 6062 8
Size: 1629 43
Fragments arranged by size:
1629 1462 1425 889 275 252 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2749 3061 3268 4027 1353
Size: 954 442 312 207 759 3423
Fragments arranged by size:
3423 954 759 442 312 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 3257 2476
Size: 781 5316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4160 4757 5544 5812 1239
Size: 393 2528 597 787 268 1524
Fragments arranged by size:
2528 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2187 2878 2187
Size: 691 5406
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2347 2379 2833
Size: 32 135 32 489 32 254 32 454
Cuts at: 2833 2865 4738 4770 4812 4844 1373
Size: 32 1873 32 42 32 2626
Fragments arranged by size:
2626 1873 489 454 254 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2950 3441 3687 669
Size: 988 281 1012 491 246 3079
Fragments arranged by size:
3079 1012 988 491 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 3687 3687
Size: 6097
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2724 2783 3082 5211 5395 1305
Size: 1419 59 299 2129 184 2007
Fragments arranged by size:
2129 2007 1419 299 184 59
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4275
Fragments arranged by size:
4275 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2943 3372 4055 4414 4573 518
Size: 648 1777 429 683 359 159 2042
Fragments arranged by size:
2042 1777 683 648 429 359 159
MspA1I CmG'CkG
Cuts at: 1606 3513 4190 5338 5583 1606
Size: 1907 677 1148 245 2120
Fragments arranged by size:
2120 1907 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4588
Fragments arranged by size:
4588 957 552
NdeI CA'TA_TG
Cuts at: 387 2305 3142 387
Size: 1918 837 3342
Fragments arranged by size:
3342 1918 837
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NsiI A_TGCA'T
Cuts at: 3148 3148
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 3455 5000 1132
Size: 97 2226 1545 2229
Fragments arranged by size:
2229 2226 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6097
PfoI T'CCnGG_A
Cuts at: 2046 2046
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2871 2903 4166 4198 5679 5711 2871
Size: 32 1263 32 1481 32 3257
Fragments arranged by size:
3257 1481 1263 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
SacI G_AGCT'C
Cuts at: 2478 3259 2478
Size: 781 5316
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 2335 4345 6057 2335
Size: 2010 1712 2375
Fragments arranged by size:
2375 2010 1712
SfcI C'TryA_G
Cuts at: 826 1045 3003 3445 4580 5261 5452 826
Size: 219 1958 442 1135 681 191 1471
Fragments arranged by size:
1958 1471 1135 681 442 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 4201 5102 5364 820
Size: 197 926 399 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 399 262 197
SnaBI TAC'GTA
Cuts at: 493 3394 3888 493
Size: 2901 494 2702
Fragments arranged by size:
2901 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 2615 4021 5
Size: 47 2563 1406 2081
Fragments arranged by size:
2563 2081 1406 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2022 2749 3536 513
Size: 552 957 727 787 3074
Fragments arranged by size:
3074 957 787 727 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2587 3258 4124 4141 4294 4479 4924
Size: 1255 1007 671 866 17 153 185 445
Cuts at: 4924 325
Size: 1498
Fragments arranged by size:
1498 1255 1007 866 671 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2333 2526 3074
Size: 274 80 33 51 1443 356 193 548
Cuts at: 3074 3185 4343 6055 96
Size: 111 1158 1712 138
Fragments arranged by size:
1712 1443 1158 548 356 274 193 138
111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 3012 4226 3012
Size: 1214 4883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI