Restriction map for
pFN21ASDA1617
(Circular) (Six-base) MAPSORT of: pFN21ASDA1617.seq Check: 6870 from: 1 to: 7411
pFN21ASDA1617 7411 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5221 278
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4754 1937
Size: 2817 4594
Acc65I G'GTAC_C
Cuts at: 4732 4732
Size: 7411
AclI AA'CG_TT
Cuts at: 1563 5538 5911 1563
Size: 3975 373 3063
Fragments arranged by size:
3975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2142 2588 2775 3842 4649 5474 6858 7126
Size: 510 446 187 1067 807 825 1384 268
Cuts at: 7126 1632
Size: 1917
Fragments arranged by size:
1917 1384 1067 825 807 510 446 268
187
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7214
AflIII A'CryG_T
Cuts at: 1203 2215 2289 6310 7287 1203
Size: 1012 74 4021 977 1327
Fragments arranged by size:
4021 1327 1012 977 74
AgeI A'CCGG_T
Cuts at: 6242 6242
Size: 7411
AhdI GACnn_n'nnGTC
Cuts at: 4264 6140 4264
Size: 1876 5535
AleI CACnn'nnGTG
Cuts at: 1166 4505 1166
Size: 3339 4072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3719 3913 1184
Size: 2535 194 4682
Fragments arranged by size:
4682 2535 194
AlwNI CAG_nnn'CTG
Cuts at: 2332 3527 5154 6726 2332
Size: 1195 1627 1572 3017
Fragments arranged by size:
3017 1627 1572 1195
ApaLI G'TGCA_C
Cuts at: 2422 5468 6624 2422
Size: 3046 1156 3209
Fragments arranged by size:
3209 3046 1156
ApoI r'AATT_y
Cuts at: 1493 2489 2864 3802 3892 3934 4720 4946 5098
Size: 996 375 938 90 42 786 226 152
Cuts at: 5098 7207 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 996 938 786 375 226 152
90 42
AseI AT'TA_AT
Cuts at: 160 1032 5965 160
Size: 872 4933 1606
Fragments arranged by size:
4933 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 4165 4424 4736 1870
Size: 73 2222 259 312 4545
Fragments arranged by size:
4545 2222 312 259 73
BamHI G'GATC_C
Cuts at: 4741 5108 4741
Size: 367 7044
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3105 4276 4372 4732 6187
Size: 325 217 627 1318 1171 96 360 1455
Cuts at: 6187 618
Size: 1842
Fragments arranged by size:
1842 1455 1318 1171 627 360 325 217
96
BanII G_rGCy'C
Cuts at: 1391 2964 4166 4242 4329 4730 1391
Size: 1573 1202 76 87 401 4072
Fragments arranged by size:
4072 1573 1202 401 87 76
BbeI G_GCGC'C
Cuts at: 4376 4376
Size: 7411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4131 928
Size: 971 2232 4208
Fragments arranged by size:
4208 2232 971
BbvCI CC'TCA_GC
Cuts at: 3609 4387 3609
Size: 778 6633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5303 6513 5303
Size: 1210 6201
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4544 6022 136
Size: 107 122 71 631 605 2872 1478 1525
Fragments arranged by size:
2872 1525 1478 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4032 7406 4032
Size: 3374 4037
BlpI GC'TnA_GC
Cuts at: 2092 4828 2092
Size: 2736 4675
Bme1580I G_kGCm'C
Cuts at: 1790 2426 4281 4412 4540 5472 6628 1790
Size: 636 1855 131 128 932 1156 2573
Fragments arranged by size:
2573 1855 1156 932 636 131 128
BmgBI CAC'GTC
Cuts at: 1318 4507 1318
Size: 3189 4222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2167 2305 3175
Size: 416 53 83 399 24 750 138 870
Cuts at: 3175 3404 4687 5142 6100 442
Size: 229 1283 455 958 1753
Fragments arranged by size:
1753 1283 958 870 750 455 416 399
229 138 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3997 4249 4297 3143
Size: 854 252 48 6257
Fragments arranged by size:
6257 854 252 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4493 6071 1239
Size: 3254 1578 2579
Fragments arranged by size:
3254 2579 1578
Bpu10I CC'TnA_GC
Cuts at: 3609 4387 3609
Size: 778 6633
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 2960 5536 6401 6699 2116
Size: 844 2576 865 298 2828
Fragments arranged by size:
2828 2576 865 844 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2103 4220 6074 882
Size: 617 604 2117 1854 2219
Fragments arranged by size:
2219 2117 1854 617 604
BsaAI yAC'GTr
Cuts at: 493 1204 2292 2319 2501 4708 5202 493
Size: 711 1088 27 182 2207 494 2702
Fragments arranged by size:
2702 2207 1088 711 494 182 27
BsaBI GATnn'nnATC
Cuts at: 7405 7405
Size: 7411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4197 4373 5218 5600
Size: 53 83 186 936 2664 176 845 382
Cuts at: 5600 6235 275
Size: 635 1451
Fragments arranged by size:
2664 1451 936 845 635 382 186 176
83 53
BsaWI w'CCGG_w
Cuts at: 5844 6242 6516 6663 7074 5844
Size: 398 274 147 411 6181
Fragments arranged by size:
6181 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7186
Fragments arranged by size:
7186 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3123 4199 4238 4375 4466 4469 4472 1186
Size: 1937 1076 39 137 91 3 3 4125
Fragments arranged by size:
4125 1937 1076 137 91 39 3 3
BseYI C'CCAG_C
Cuts at: 4633 6614 7110 4633
Size: 1981 496 4934
Fragments arranged by size:
4934 1981 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3432 4142 4631 1163
Size: 573 1696 710 489 3943
Fragments arranged by size:
3943 1696 710 573 489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4861 5622 6650 1540
Size: 275 186 2860 761 1028 2301
Fragments arranged by size:
2860 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2033 2426 4242 5472 5557 6628 2033
Size: 393 1816 1230 85 1071 2816
Fragments arranged by size:
2816 1816 1230 1071 393 85
BsiWI C'GTAC_G
Cuts at: 3868 3868
Size: 7411
BsmI GAATG_Cn'
Cuts at: 2229 4922 5015 2229
Size: 2693 93 4625
Fragments arranged by size:
4625 2693 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3809 3809
Size: 7411
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2426 2964 4166 4242 4281 4329
Size: 399 243 393 538 1202 76 39 48
Cuts at: 4329 4412 4540 4730 5472 5557 6628 1391
Size: 83 128 190 742 85 1071 2174
Fragments arranged by size:
2174 1202 1071 742 538 399 393 243
190 128 85 83 76 48 39
BspEI T'CCGG_A
Cuts at: 7074 7074
Size: 7411
BspHI T'CATG_A
Cuts at: 3544 5300 7030 3544
Size: 1756 1730 3925
Fragments arranged by size:
3925 1756 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4766 844
Size: 3922 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 5298 1394
Size: 429 2494 981 3507
Fragments arranged by size:
3507 2494 981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5906 6080 65
Size: 1183 4658 174 1396
Fragments arranged by size:
4658 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4229 4335 4375 6055 6242 1700
Size: 110 2419 106 40 1680 187 2869
Fragments arranged by size:
2869 2419 1680 187 110 106 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7411
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 5471 6483 5471
Size: 1012 6399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2192 2332 794
Size: 1398 140 5873
Fragments arranged by size:
5873 1398 140
BstBI TT'CG_AA
Cuts at: 2007 6215 2007
Size: 4208 3203
BstEII G'GTnAC_C
Cuts at: 1184 4498 1184
Size: 3314 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2754 3102 3417 2539
Size: 215 348 315 6533
Fragments arranged by size:
6533 348 315 215
BstYI r'GATC_y
Cuts at: 1972 3790 4032 4741 5108 5493 5510 6962 7048
Size: 1818 242 709 367 385 17 1452 86
Cuts at: 7048 7060 7406 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1818 1452 709 385 367 346 242
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2412 3396 4146 4158 4200 4366
Size: 552 957 390 984 750 12 42 166
Cuts at: 4366 513
Size: 3558
Fragments arranged by size:
3558 984 957 750 552 390 166 42
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2380 487
Size: 683 56 1154 5518
Fragments arranged by size:
5518 1154 683 56
BtsI GCAGTG_nn'
Cuts at: 4927 5721 5741 4927
Size: 794 20 6597
Fragments arranged by size:
6597 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7376
DraI TTT'AAA
Cuts at: 4715 4715
Size: 7411
DraIII CAC_nnn'GTG
Cuts at: 2413 2413
Size: 7411
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6418 809
Size: 507 5102 1802
Fragments arranged by size:
5102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 3151 4337
Size: 54 1425 50 275 18 471 850 1186
Cuts at: 4337 4545 4576 4858 5747 7376 8
Size: 208 31 282 889 1629 43
Fragments arranged by size:
1629 1425 1186 889 850 471 282 275
208 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 1537
Size: 275 3046 4090
Fragments arranged by size:
4090 3046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2877 3470 3620 3807 4176 5341 1353
Size: 816 708 593 150 187 369 1165 3423
Fragments arranged by size:
3423 1165 816 708 593 369 187 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4286 5998 6372 6518 387
Size: 1064 138 2519 178 1712 374 146 1280
Fragments arranged by size:
2519 1712 1280 1064 374 178 146 138
EcoICRI GAG'CTC
Cuts at: 4240 4240
Size: 7411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2588 2775 3842 4493 4649 5474
Size: 393 510 446 187 1067 651 156 825
Cuts at: 5474 6071 6858 7126 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1067 825 787 651 597 510 446
393 268 187 156
EcoNI CCTnn'n_nnAGG
Cuts at: 2553 3138 2553
Size: 585 6826
EcoO109I rG'GnC_Cy
Cuts at: 2546 2564 3641 4637 2546
Size: 18 1077 996 5320
Fragments arranged by size:
5320 1077 996 18
EcoRI G'AATT_C
Cuts at: 4720 5098 4720
Size: 378 7033
EcoRV GAT'ATC
Cuts at: 3957 3957
Size: 7411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3740 3740
Size: 7411
FspI TGC'GCA
Cuts at: 5917 5917
Size: 7411
HaeII r_GCGC'y
Cuts at: 1678 4376 6558 1678
Size: 2698 2182 2531
Fragments arranged by size:
2698 2531 2182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2687 2719 3377
Size: 32 135 32 476 32 607 32 658
Cuts at: 3377 3409 4250 4282 6052 6084 6126 6158 1373
Size: 32 841 32 1770 32 42 32 2626
Fragments arranged by size:
2626 1770 841 658 607 476 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3589 4755 5001 669
Size: 988 281 1651 1166 246 3079
Fragments arranged by size:
3079 1651 1166 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2134 2936 748
Size: 1386 802 5223
Fragments arranged by size:
5223 1386 802
HpaI GTT'AAC
Cuts at: 5001 5001
Size: 7411
KasI G'GCGC_C
Cuts at: 4372 4372
Size: 7411
KpnI G_GTAC'C
Cuts at: 4736 4736
Size: 7411
MfeI C'AATT_G
Cuts at: 3509 5010 3509
Size: 1501 5910
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2638 4467 4628 6525 6709 1305
Size: 1333 1829 161 1897 184 2007
Fragments arranged by size:
2007 1897 1829 1333 184 161
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 3153 4547 10
Size: 54 1425 343 471 850 1394 2874
Fragments arranged by size:
2874 1425 1394 850 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4118 4505 5369 5728 5887 518
Size: 648 2952 387 864 359 159 2042
Fragments arranged by size:
2952 2042 864 648 387 359 159
MspA1I CmG'CkG
Cuts at: 1606 2329 3781 4071 4160 4368 4827 5504 6652
Size: 723 1452 290 89 208 459 677 1148
Cuts at: 6652 6897 1606
Size: 245 2120
Fragments arranged by size:
2120 1452 1148 723 677 459 290 245
208 89
NaeI GCC'GGC
Cuts at: 4337 4377 4337
Size: 40 7371
NarI GG'CG_CC
Cuts at: 4373 4373
Size: 7411
NcoI C'CATG_G
Cuts at: 513 1065 2022 3396 4200 513
Size: 552 957 1374 804 3724
Fragments arranged by size:
3724 1374 957 804 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7411
NgoMIV G'CCGG_C
Cuts at: 4335 4375 4335
Size: 40 7371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NspI r_CATG'y
Cuts at: 1132 1229 4769 6314 1132
Size: 97 3540 1545 2229
Fragments arranged by size:
3540 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7411
PciI A'CATG_T
Cuts at: 6310 6310
Size: 7411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7411
PfoI T'CCnGG_A
Cuts at: 2538 3286 3807 4242 2538
Size: 748 521 435 5707
Fragments arranged by size:
5707 748 521 435
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PmlI CAC'GTG
Cuts at: 2319 2319
Size: 7411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5480 5512 6993 7025 5480
Size: 32 1481 32 5866
Fragments arranged by size:
5866 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 2546
Size: 7411
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7411
PsiI TTA'TAA
Cuts at: 4981 4981
Size: 7411
PstI C_TGCA'G
Cuts at: 830 3360 4763 830
Size: 2530 1403 3478
Fragments arranged by size:
3478 2530 1403
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2329 3781 2329
Size: 1452 5959
SacI G_AGCT'C
Cuts at: 4242 4242
Size: 7411
SacII CC_GC'GG
Cuts at: 4161 4369 4161
Size: 208 7203
SalI G'TCGA_C
Cuts at: 1936 4753 1936
Size: 2817 4594
SapI GCTCTTCn'nnn_
Cuts at: 3620 3620
Size: 7411
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 2630 5659 7371 2630
Size: 3029 1712 2670
Fragments arranged by size:
3029 2670 1712
SfcI C'TryA_G
Cuts at: 826 1045 2568 3356 4759 5894 6575 6766 826
Size: 219 1523 788 1403 1135 681 191 1471
Fragments arranged by size:
1523 1471 1403 1135 788 681 219 191
SfoI GGC'GCC
Cuts at: 4374 4374
Size: 7411
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 4738 1872
Size: 2866 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2131 2939 5515 6416 6678 820
Size: 197 926 188 808 2576 901 262 1553
Fragments arranged by size:
2576 1553 926 901 808 262 197 188
SnaBI TAC'GTA
Cuts at: 493 4708 5202 493
Size: 4215 494 2702
Fragments arranged by size:
4215 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7411
SphI G_CATG'C
Cuts at: 1132 4769 1132
Size: 3637 3774
SspI AAT'ATT
Cuts at: 5 52 2168 5335 5
Size: 47 2116 3167 2081
Fragments arranged by size:
3167 2116 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7411
StyI C'CwwG_G
Cuts at: 513 1065 2022 2073 3396 4200 4542 4850 513
Size: 552 957 51 1323 804 342 308 3074
Fragments arranged by size:
3074 1323 957 804 552 342 308 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2287 5438 5455 5608 5793 6238 325
Size: 1255 707 3151 17 153 185 445 1498
Fragments arranged by size:
3151 1498 1255 707 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2472 2628 2831
Size: 274 80 33 51 1443 495 156 203
Cuts at: 2831 3097 5657 7369 96
Size: 266 2560 1712 138
Fragments arranged by size:
2560 1712 1443 495 274 266 203 156
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 7371 7403 2626
Size: 32 4713 32 2634
Fragments arranged by size:
4713 2634 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2390 3884 1316
Size: 1074 1494 4843
Fragments arranged by size:
4843 1494 1074
XbaI T'CTAG_A
Cuts at: 4747 4747
Size: 7411
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7411
XmaI C'CCGG_G
Cuts at: 1870 4736 1870
Size: 2866 4545
XmnI GAAnn'nnTTC
Cuts at: 5540 5540
Size: 7411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5219 276
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI
XcmI