Restriction map for
pFN21ASDA1587
(Circular) (Six-base) MAPSORT of: pFN21ASDA1587.seq Check: 3167 from: 1 to: 7600
pFN21ASDA1587 7600 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2157 5410 278
Size: 53 83 186 936 621 3253 2468
Fragments arranged by size:
3253 2468 936 621 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4503 4943 1937
Size: 2566 440 4594
Fragments arranged by size:
4594 2566 440
Acc65I G'GTAC_C
Cuts at: 2497 4183 4921 2497
Size: 1686 738 5176
Fragments arranged by size:
5176 1686 738
AclI AA'CG_TT
Cuts at: 1563 5727 6100 1563
Size: 4164 373 3063
Fragments arranged by size:
4164 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3497 4049 4873 5663 7047 7315 1632
Size: 1865 552 824 790 1384 268 1917
Fragments arranged by size:
1917 1865 1384 824 790 552 268
AfeI AGC'GCT
Cuts at: 4667 4667
Size: 7600
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7403
AflIII A'CryG_T
Cuts at: 1203 6499 7476 1203
Size: 5296 977 1327
Fragments arranged by size:
5296 1327 977
AgeI A'CCGG_T
Cuts at: 6431 6431
Size: 7600
AhdI GACnn_n'nnGTC
Cuts at: 6329 6329
Size: 7600
AleI CACnn'nnGTG
Cuts at: 1166 2258 2330 2402 2474 2546 2582 2618 2654
Size: 1092 72 72 72 72 36 36 36
Cuts at: 2654 2690 2726 2762 2798 2834 2906 2942 2978
Size: 36 36 36 36 36 72 36 36
Cuts at: 2978 3014 3050 3122 3158 3194 1166
Size: 36 36 72 36 36 5572
Fragments arranged by size:
5572 1092 72 72 72 72 72 72
36 36 36 36 36 36 36 36
36 36 36 36 36 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2276 2348 2492 2528 2564 2636 2672 2708
Size: 1092 72 144 36 36 72 36 36
Cuts at: 2708 2744 2780 2816 2852 2924 2960 2970 2996
Size: 36 36 36 36 72 36 10 26
Cuts at: 2996 3032 3068 3140 3212 1184
Size: 36 36 72 72 5572
Fragments arranged by size:
5572 1092 144 72 72 72 72 72
36 36 36 36 36 36 36 36
36 36 36 26 10
AlwNI CAG_nnn'CTG
Cuts at: 2094 3362 3526 3953 5343 6915 2094
Size: 1268 164 427 1390 1572 2779
Fragments arranged by size:
2779 1572 1390 1268 427 164
ApaI G_GGCC'C
Cuts at: 2213 2249 3257 3992 2213
Size: 36 1008 735 5821
Fragments arranged by size:
5821 1008 735 36
ApaLI G'TGCA_C
Cuts at: 2395 5657 6813 2395
Size: 3262 1156 3182
Fragments arranged by size:
3262 3182 1156
ApoI r'AATT_y
Cuts at: 1493 2041 3395 4023 4332 4909 5135 5287 7396
Size: 548 1354 628 309 577 226 152 2109
Cuts at: 7396 1493
Size: 1697
Fragments arranged by size:
2109 1697 1354 628 577 548 309 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6154 160
Size: 872 5122 1606
Fragments arranged by size:
5122 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7600
AvaI C'yCGr_G
Cuts at: 1870 1943 3256 3459 4925 1870
Size: 73 1313 203 1466 4545
Fragments arranged by size:
4545 1466 1313 203 73
AvrII C'CTAG_G
Cuts at: 3329 3329
Size: 7600
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4645 4678 4645
Size: 33 7567
BamHI G'GATC_C
Cuts at: 4930 5297 4930
Size: 367 7233
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 2497 2587 2648 2875
Size: 325 217 627 537 173 90 61 227
Cuts at: 2875 4183 4921 6376 618
Size: 1308 738 1455 1842
Fragments arranged by size:
1842 1455 1308 738 627 537 325 227
217 173 90 61
BanII G_rGCy'C
Cuts at: 1391 2213 2249 3257 3425 3749 3992 4738 4919
Size: 822 36 1008 168 324 243 746 181
Cuts at: 4919 1391
Size: 4072
Fragments arranged by size:
4072 1008 822 746 324 243 181 168
36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6629
BbvCI CC'TCA_GC
Cuts at: 2241 4122 2241
Size: 1881 5719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5791 5825 1903
Size: 34 3854 34 3678
Fragments arranged by size:
3854 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5492 6702 5492
Size: 1210 6390
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7600
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3821 6211 136
Size: 107 122 71 631 605 2149 2390 1525
Fragments arranged by size:
2390 2149 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7595 7595
Size: 7600
BlpI GC'TnA_GC
Cuts at: 3510 5017 3510
Size: 1507 6093
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2249 2399 2592 2880 2913 3257 3726
Size: 423 36 150 193 288 33 344 469
Cuts at: 3726 3992 4866 5661 6817 1790
Size: 266 874 795 1156 2573
Fragments arranged by size:
2573 1156 874 795 469 423 344 288
266 193 150 36 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 3722 5331
Size: 416 53 83 399 24 923 1382 1609
Cuts at: 5331 6289 442
Size: 958 1753
Fragments arranged by size:
1753 1609 1382 958 923 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2422 3662 3865 6260 1239
Size: 932 251 1240 203 2395 2579
Fragments arranged by size:
2579 2395 1240 932 251 203
Bpu10I CC'TnA_GC
Cuts at: 2241 2357 2681 2753 2933 4122 4547 4612 2241
Size: 116 324 72 180 1189 425 65 5229
Fragments arranged by size:
5229 1189 425 324 180 116 72 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 4678 5725 6590 6888 2269
Size: 2409 1047 865 298 2981
Fragments arranged by size:
2981 2409 1047 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 6263 882
Size: 617 633 4131 2219
Fragments arranged by size:
4131 2219 633 617
BsaAI yAC'GTr
Cuts at: 493 1204 4897 5391 493
Size: 711 3693 494 2702
Fragments arranged by size:
3693 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7594 7594
Size: 7600
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 5407 5789 6424
Size: 53 83 186 936 621 3253 382 635
Cuts at: 6424 275
Size: 1451
Fragments arranged by size:
3253 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3291 6033 6431 6705 6852 7263 3291
Size: 2742 398 274 147 411 3628
Fragments arranged by size:
3628 2742 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3962 3992 1178
Size: 30 165 30 2559 30 4786
Fragments arranged by size:
4786 2559 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3050 4508 1186
Size: 1864 1458 4278
Fragments arranged by size:
4278 1864 1458
BseYI C'CCAG_C
Cuts at: 2094 2168 4625 6803 7299 2094
Size: 74 2457 2178 496 2395
Fragments arranged by size:
2457 2395 2178 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 2966 3218 3399 3878 4301 4378
Size: 573 978 252 252 181 479 423 77
Cuts at: 4378 1163
Size: 4385
Fragments arranged by size:
4385 978 573 479 423 252 252 181
77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5050 5811 6839 1540
Size: 275 186 3049 761 1028 2301
Fragments arranged by size:
3049 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2399 2507 2543 2687 2723 2795 2831 2939 2975
Size: 108 36 144 36 72 36 108 36
Cuts at: 2975 3011 3047 3119 3263 5661 5746 6817 2399
Size: 36 36 72 144 2398 85 1071 3182
Fragments arranged by size:
3182 2398 1071 144 144 108 108 85
72 72 36 36 36 36 36 36
BsmI GAATG_Cn'
Cuts at: 5111 5204 5111
Size: 93 7507
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2249 2399 2507 2543 2592 2687
Size: 399 423 36 150 108 36 49 95
Cuts at: 2687 2723 2795 2831 2880 2913 2939 2975 3011
Size: 36 72 36 49 33 26 36 36
Cuts at: 3011 3047 3119 3257 3263 3425 3726 3749 3992
Size: 36 72 138 6 162 301 23 243
Cuts at: 3992 4738 4866 4919 5661 5746 6817 1391
Size: 746 128 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 423 399 301 243
162 150 138 128 108 95 85 72
72 53 49 49 36 36 36 36
36 36 36 33 26 23 6
BspEI T'CCGG_A
Cuts at: 7263 7263
Size: 7600
BspHI T'CATG_A
Cuts at: 5489 7219 5489
Size: 1730 5870
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4531 4655 4955 844
Size: 3687 124 300 3489
Fragments arranged by size:
3687 3489 300 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2662 5487 1394
Size: 429 839 2825 3507
Fragments arranged by size:
3507 2825 839 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4836 6095 6269 65
Size: 1183 3588 1259 174 1396
Fragments arranged by size:
3588 1396 1259 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2803 6244 6431 1700
Size: 110 993 3441 187 2869
Fragments arranged by size:
3441 2869 993 187 110
BsrGI T'GTAC_A
Cuts at: 96 4464 96
Size: 4368 3232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7600
BssSI C'ACGA_G
Cuts at: 5660 6672 5660
Size: 1012 6588
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3526 794
Size: 2732 4868
BstBI TT'CG_AA
Cuts at: 2007 6404 2007
Size: 4397 3203
BstEII G'GTnAC_C
Cuts at: 1184 3334 1184
Size: 2150 5450
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 4239 4415 4806 2029
Size: 2210 176 391 4823
Fragments arranged by size:
4823 2210 391 176
BstYI r'GATC_y
Cuts at: 1972 2181 3995 4057 4930 5297 5682 5699 7151
Size: 209 1814 62 873 367 385 17 1452
Cuts at: 7151 7237 7249 7595 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1814 1452 873 385 367 346 209
86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 3372 3681 3372
Size: 309 7291
BtgI C'CryG_G
Cuts at: 513 1065 2583 2884 3348 513
Size: 552 1518 301 464 4765
Fragments arranged by size:
4765 1518 552 464 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6861
Fragments arranged by size:
6861 683 56
BtsI GCAGTG_nn'
Cuts at: 2952 5116 5910 5930 2952
Size: 2164 794 20 4622
Fragments arranged by size:
4622 2164 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7600
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7565
DraI TTT'AAA
Cuts at: 4904 4904
Size: 7600
DraIII CAC_nnn'GTG
Cuts at: 2636 2636
Size: 7600
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6607 809
Size: 507 5291 1802
Fragments arranged by size:
5291 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2805 4070 4348
Size: 54 1425 50 275 18 975 1265 278
Cuts at: 4348 4776 4808 5047 5936 7565 8
Size: 428 32 239 889 1629 43
Fragments arranged by size:
1629 1425 1265 975 889 428 278 275
239 54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 5047 1537
Size: 275 3235 4090
Fragments arranged by size:
4090 3235 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3735 5530 1353
Size: 2382 1795 3423
Fragments arranged by size:
3423 2382 1795
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6187 6561 6707 387
Size: 1064 138 4598 374 146 1280
Fragments arranged by size:
4598 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2422 3497 3662 3865 4049 4873
Size: 393 539 251 1075 165 203 184 824
Cuts at: 4873 5663 6260 7047 7315 1239
Size: 790 597 787 268 1524
Fragments arranged by size:
1524 1075 824 790 787 597 539 393
268 251 203 184 165
EcoO109I rG'GnC_Cy
Cuts at: 2110 2165 2191 2209 2245 2318 2353 2677 2749
Size: 55 26 18 36 73 35 324 72
Cuts at: 2749 2929 3253 3368 3386 3403 3716 3988 4155
Size: 180 324 115 18 17 313 272 167
Cuts at: 4155 4543 4727 2110
Size: 388 184 4983
Fragments arranged by size:
4983 388 324 324 313 272 184 180
167 115 73 72 55 36 35 26
18 18 17
EcoRI G'AATT_C
Cuts at: 2041 3395 4332 4909 5287 2041
Size: 1354 937 577 378 4354
Fragments arranged by size:
4354 1354 937 577 378
FspI TGC'GCA
Cuts at: 2082 6106 2082
Size: 4024 3576
HaeII r_GCGC'y
Cuts at: 1678 2292 4669 6747 1678
Size: 614 2377 2078 2531
Fragments arranged by size:
2531 2377 2078 614
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2850 2882 4172 4204 6241
Size: 32 135 32 1278 32 1290 32 2037
Cuts at: 6241 6273 6315 6347 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2037 1290 1278 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4944 5190 669
Size: 988 281 3006 246 3079
Fragments arranged by size:
3079 3006 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4549 748
Size: 3801 3799
HpaI GTT'AAC
Cuts at: 5190 5190
Size: 7600
KpnI G_GTAC'C
Cuts at: 2501 4187 4925 2501
Size: 1686 738 5176
Fragments arranged by size:
5176 1686 738
MfeI C'AATT_G
Cuts at: 5199 5199
Size: 7600
MluI A'CGCG_T
Cuts at: 7476 7476
Size: 7600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6714 6898 1305
Size: 5409 184 2007
Fragments arranged by size:
5409 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4072 4778 4810 10
Size: 54 1425 343 2240 706 32 2800
Fragments arranged by size:
2800 2240 1425 706 343 54 32
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2330 2402 2474 2546 2582 2618
Size: 648 1092 72 72 72 72 36 36
Cuts at: 2618 2654 2690 2726 2762 2798 2834 2906 2942
Size: 36 36 36 36 36 36 72 36
Cuts at: 2942 2978 3014 3050 3122 3158 3194 4016 4237
Size: 36 36 36 72 36 36 822 221
Cuts at: 4237 4413 5558 5917 6076 518
Size: 176 1145 359 159 2042
Fragments arranged by size:
2042 1145 1092 822 648 359 221 176
159 72 72 72 72 72 72 36
36 36 36 36 36 36 36 36
36 36 36 36 36
MspA1I CmG'CkG
Cuts at: 1606 2094 2135 2240 2886 3862 5016 5693 6841
Size: 488 41 105 646 976 1154 677 1148
Cuts at: 6841 7086 1606
Size: 245 2120
Fragments arranged by size:
2120 1154 1148 976 677 646 488 245
105 41
NaeI GCC'GGC
Cuts at: 2805 2805
Size: 7600
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7048
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7600
NgoMIV G'CCGG_C
Cuts at: 2803 2803
Size: 7600
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7600
NotI GC'GGCC_GC
Cuts at: 5047 5047
Size: 7600
NspI r_CATG'y
Cuts at: 1132 1229 4640 4958 6503 1132
Size: 97 3411 318 1545 2229
Fragments arranged by size:
3411 2229 1545 318 97
PasI CC'CwG_GG
Cuts at: 1348 2161 1348
Size: 813 6787
PciI A'CATG_T
Cuts at: 6499 6499
Size: 7600
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7600
PfoI T'CCnGG_A
Cuts at: 3558 3558
Size: 7600
PmeI GTTT'AAAC
Cuts at: 4904 4904
Size: 7600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 5669 5701 7182 7214 2011
Size: 32 3626 32 1481 32 2397
Fragments arranged by size:
3626 2397 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2165 2318 3368 3403 3716 4543 4727 2165
Size: 153 1050 35 313 827 184 5038
Fragments arranged by size:
5038 1050 827 313 184 153 35
PshAI GACnn'nnGTC
Cuts at: 1613 3904 1613
Size: 2291 5309
PsiI TTA'TAA
Cuts at: 5170 5170
Size: 7600
PspOMI G'GGCC_C
Cuts at: 2209 2245 3253 3988 2209
Size: 36 1008 735 5821
Fragments arranged by size:
5821 1008 735 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4416 4448 4416
Size: 32 7568
PstI C_TGCA'G
Cuts at: 830 4180 4591 4652 4952 830
Size: 3350 411 61 300 3478
Fragments arranged by size:
3478 3350 411 300 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7600
PvuII CAG'CTG
Cuts at: 2094 2094
Size: 7600
RsrII CG'GwC_CG
Cuts at: 3218 3218
Size: 7600
SacII CC_GC'GG
Cuts at: 2887 2887
Size: 7600
SalI G'TCGA_C
Cuts at: 1936 4942 1936
Size: 3006 4594
SanDI GG'GwC_CC
Cuts at: 2165 2165
Size: 7600
SbfI CC_TGCA'GG
Cuts at: 4952 4952
Size: 7600
ScaI AGT'ACT
Cuts at: 5848 7560 5848
Size: 1712 5888
SexAI A'CCwGG_T
Cuts at: 2632 2632
Size: 7600
SfcI C'TryA_G
Cuts at: 826 1045 3390 3940 4176 4587 4648 4948 6083
Size: 219 2345 550 236 411 61 300 1135
Cuts at: 6083 6764 6955 826
Size: 681 191 1471
Fragments arranged by size:
2345 1471 1135 681 550 411 300 236
219 191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7600
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7600
SmaI CCC'GGG
Cuts at: 1872 3461 4927 1872
Size: 1589 1466 4545
Fragments arranged by size:
4545 1589 1466
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 4657 5704 6605 6867 820
Size: 197 926 341 2373 1047 901 262 1553
Fragments arranged by size:
2373 1553 1047 926 901 341 262 197
SnaBI TAC'GTA
Cuts at: 493 4897 5391 493
Size: 4404 494 2702
Fragments arranged by size:
4404 2702 494
SpeI A'CTAG_T
Cuts at: 152 4426 152
Size: 4274 3326
SphI G_CATG'C
Cuts at: 1132 4958 1132
Size: 3826 3774
SspI AAT'ATT
Cuts at: 5 52 3795 5524 5
Size: 47 3743 1729 2081
Fragments arranged by size:
3743 2081 1729 47
StuI AGG'CCT
Cuts at: 1517 2282 3344 4519 1517
Size: 765 1062 1175 4598
Fragments arranged by size:
4598 1175 1062 765
StyI C'CwwG_G
Cuts at: 513 1065 2871 3329 3339 3816 5039 513
Size: 552 1806 458 10 477 1223 3074
Fragments arranged by size:
3074 1806 1223 552 477 458 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2731 2785 2803 2839 5627 5644 5797
Size: 1255 1151 54 18 36 2788 17 153
Cuts at: 5797 5982 6427 325
Size: 185 445 1498
Fragments arranged by size:
2788 1498 1255 1151 445 185 153 54
36 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4338 4464 4818
Size: 274 80 33 51 1443 2361 126 354
Cuts at: 4818 5846 7558 96
Size: 1028 1712 138
Fragments arranged by size:
2361 1712 1443 1028 354 274 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3216 3248 7560 7592 3216
Size: 32 4312 32 3224
Fragments arranged by size:
4312 3224 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7600
XbaI T'CTAG_A
Cuts at: 4936 4936
Size: 7600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3346 3439 3346
Size: 93 7507
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7600
XmaI C'CCGG_G
Cuts at: 1870 3459 4925 1870
Size: 1589 1466 4545
Fragments arranged by size:
4545 1589 1466
XmnI GAAnn'nnTTC
Cuts at: 5729 5729
Size: 7600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2155 5408 276
Size: 53 83 186 936 621 3253 2468
Fragments arranged by size:
3253 2468 936 621 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI SacI SapI
SfiI SfoI SrfI SwaI