Restriction map for
pFN21ASDA1581
(Circular) (Six-base) MAPSORT of: pFN21ASDA1581.seq Check: 6527 from: 1 to: 6205
pFN21ASDA1581 6205 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3375 3548 1937
Size: 1438 173 4594
Fragments arranged by size:
4594 1438 173
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3224 4268 5652 5920 1632
Size: 1592 1044 1384 268 1917
Fragments arranged by size:
1917 1592 1384 1044 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 3063 5104 6081 1203
Size: 1860 2041 977 1327
Fragments arranged by size:
2041 1860 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 3948 5520 3948
Size: 1572 4633
ApaLI G'TGCA_C
Cuts at: 3290 4262 5418 3290
Size: 972 1156 4077
Fragments arranged by size:
4077 1156 972
ApoI r'AATT_y
Cuts at: 1493 2030 3514 3740 3892 6001 1493
Size: 537 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 537 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2811 3530 1870
Size: 73 868 719 4545
Fragments arranged by size:
4545 868 719 73
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 3526 4981 618
Size: 325 217 627 287 1452 1455 1842
Fragments arranged by size:
1842 1455 1452 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 2589 3317 3524 1391
Size: 1198 728 207 4072
Fragments arranged by size:
4072 1198 728 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3432 928
Size: 971 1533 3701
Fragments arranged by size:
3701 1533 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2345 2931 4097 5307 2345
Size: 586 1166 1210 3243
Fragments arranged by size:
3243 1210 1166 586
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2843 4816 136
Size: 107 122 71 631 605 1171 1973 1525
Fragments arranged by size:
1973 1525 1171 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 2735 3622 2735
Size: 887 5318
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3294 4266 5422 1790
Size: 289 1215 972 1156 2573
Fragments arranged by size:
2573 1215 1156 972 289
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3149 3936 4894
Size: 416 53 83 399 24 1732 787 958
Cuts at: 4894 442
Size: 1753
Fragments arranged by size:
1753 1732 958 787 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4865 1239
Size: 3626 2579
Bpu10I CC'TnA_GC
Cuts at: 2535 2535
Size: 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4330 5195 5493 4330
Size: 865 298 5042
Fragments arranged by size:
5042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3502 3996 493
Size: 711 2298 494 2702
Fragments arranged by size:
2702 2298 711 494
BsaBI GATnn'nnATC
Cuts at: 3086 6199 3086
Size: 3113 3092
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4012 4394 5029 275
Size: 53 83 186 936 2479 382 635 1451
Fragments arranged by size:
2479 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2064 3378 4638 5036 5310 5457 5868 2064
Size: 1314 1260 398 274 147 411 2401
Fragments arranged by size:
2401 1314 1260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3392 3422 1178
Size: 30 165 30 1989 30 3961
Fragments arranged by size:
3961 1989 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3473 3503 1186
Size: 2287 30 3888
Fragments arranged by size:
3888 2287 30
BseYI C'CCAG_C
Cuts at: 2704 2945 3227 5408 5904 2704
Size: 241 282 2181 496 3005
Fragments arranged by size:
3005 2181 496 282 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 2620 1163
Size: 573 621 263 4748
Fragments arranged by size:
4748 621 573 263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2966 3294 4266 4351 5422 2966
Size: 328 972 85 1071 3749
Fragments arranged by size:
3749 1071 972 328 85
BsmI GAATG_Cn'
Cuts at: 2472 2582 3716 3809 2472
Size: 110 1134 93 4868
Fragments arranged by size:
4868 1134 110 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2589 2966 3294 3317 3524 4266
Size: 399 289 510 377 328 23 207 742
Cuts at: 4266 4351 5422 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 377 328 289
207 85 23
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 2196 4094 5824 2196
Size: 1898 1730 2577
Fragments arranged by size:
2577 1898 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2133 2457 4700 4874 65
Size: 1183 885 324 2243 174 1396
Fragments arranged by size:
2243 1396 1183 885 324 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4849 5036 1700
Size: 110 3039 187 2869
Fragments arranged by size:
3039 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2613 96
Size: 2517 3688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 2965 3266 4265 5277 2965
Size: 301 999 1012 3893
Fragments arranged by size:
3893 1012 999 301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 2007 5009 2007
Size: 3002 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 2895 3268 2895
Size: 373 5832
BstYI r'GATC_y
Cuts at: 1972 3138 3535 3902 4287 4304 5756 5842 5854
Size: 1166 397 367 385 17 1452 86 12
Cuts at: 5854 6200 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1166 397 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3348 3414 513
Size: 552 957 1326 66 3304
Fragments arranged by size:
3304 1326 957 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2293 3241 3343 487
Size: 683 56 1067 948 102 3349
Fragments arranged by size:
3349 1067 948 683 102 56
BtsI GCAGTG_nn'
Cuts at: 2853 3721 4515 4535 2853
Size: 868 794 20 4523
Fragments arranged by size:
4523 868 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DraIII CAC_nnn'GTG
Cuts at: 2380 2646 3189 2380
Size: 266 543 5396
Fragments arranged by size:
5396 543 266
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2766 3411
Size: 54 1425 50 275 18 339 597 645
Cuts at: 3411 3652 4541 6170 8
Size: 241 889 1629 43
Fragments arranged by size:
1629 1425 889 645 597 339 275 241
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4135 1353
Size: 2782 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3224 4268 4865 5652 5920 1239
Size: 393 1592 1044 597 787 268 1524
Fragments arranged by size:
1592 1524 1044 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2213 2412 2815 2213
Size: 199 403 5603
Fragments arranged by size:
5603 403 199
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 3229 3022
Size: 207 5998
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2042 5352 1678
Size: 364 3310 2531
Fragments arranged by size:
3310 2531 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2767 2799 3163 3195 3392
Size: 32 135 32 1195 32 364 32 197
Cuts at: 3392 3424 4846 4878 4920 4952 1373
Size: 32 1422 32 42 32 2626
Fragments arranged by size:
2626 1422 1195 364 197 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 2901 3549 3795 669
Size: 988 281 859 104 648 246 3079
Fragments arranged by size:
3079 988 859 648 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3476 5319 5503 1305
Size: 2171 1843 184 2007
Fragments arranged by size:
2171 2007 1843 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 2768 10
Size: 54 1425 343 339 597 3447
Fragments arranged by size:
3447 1425 597 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3095 4163 4522 4681 518
Size: 648 1929 1068 359 159 2042
Fragments arranged by size:
2042 1929 1068 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3621 4298 5446 5691 1606
Size: 2015 677 1148 245 2120
Fragments arranged by size:
2120 2015 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 3348 513
Size: 552 957 1326 3370
Fragments arranged by size:
3370 1326 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2887 3448 3563 5108 1132
Size: 97 1658 561 115 1545 2229
Fragments arranged by size:
2229 1658 1545 561 115 97
PasI CC'CwG_GG
Cuts at: 1348 2080 3304 1348
Size: 732 1224 4249
Fragments arranged by size:
4249 1224 732
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6205
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2213 2815 2213
Size: 602 5603
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PstI C_TGCA'G
Cuts at: 830 2836 3557 830
Size: 2006 721 3478
Fragments arranged by size:
3478 2006 721
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SanDI GG'GwC_CC
Cuts at: 2213 2213
Size: 6205
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2832 3553 4688 5369 5560 826
Size: 219 1787 721 1135 681 191 1471
Fragments arranged by size:
1787 1471 1135 721 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 2813 3532 1872
Size: 941 719 4545
Fragments arranged by size:
4545 941 719
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4309 5210 5472 820
Size: 197 926 2366 901 262 1553
Fragments arranged by size:
2366 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3502 3996 493
Size: 3009 494 2702
Fragments arranged by size:
3009 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 2120 1517
Size: 603 5602
StyI C'CwwG_G
Cuts at: 513 1065 2022 2818 3348 3644 513
Size: 552 957 796 530 296 3074
Fragments arranged by size:
3074 957 796 552 530 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3073 4232 4249 4402 4587 5032 325
Size: 1255 1493 1159 17 153 185 445 1498
Fragments arranged by size:
1498 1493 1255 1159 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 4451 6163
Size: 274 80 33 51 1443 636 1838 1712
Cuts at: 6163 96
Size: 138
Fragments arranged by size:
1838 1712 1443 636 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6165 6197 6165
Size: 32 6173
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2647 2845 2872 2953 2179
Size: 468 198 27 81 5431
Fragments arranged by size:
5431 468 198 81 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 2811 3530 1870
Size: 941 719 4545
Fragments arranged by size:
4545 941 719
XmnI GAAnn'nnTTC
Cuts at: 2204 3212 4334 2204
Size: 1008 1122 4075
Fragments arranged by size:
4075 1122 1008
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI