Restriction map for
pFN21ASDA1580
(Circular) (Six-base) MAPSORT of: pFN21ASDA1580.seq Check: 6480 from: 1 to: 6649
pFN21ASDA1580 6649 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3783 3783
Size: 6649
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4459 278
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 3468 3521 3992 1937
Size: 520 1011 53 471 4594
Fragments arranged by size:
4594 1011 520 471 53
Acc65I G'GTAC_C
Cuts at: 3970 3970
Size: 6649
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2492 2804 3141 3318 4712 6096 6364 1632
Size: 860 312 337 177 1394 1384 268 1917
Fragments arranged by size:
1917 1394 1384 860 337 312 268 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 3296 5548 6525 1203
Size: 2093 2252 977 1327
Fragments arranged by size:
2252 2093 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 5378 5378
Size: 6649
AleI CACnn'nnGTG
Cuts at: 1166 3461 3485 1166
Size: 2295 24 4330
Fragments arranged by size:
4330 2295 24
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6649
AlwNI CAG_nnn'CTG
Cuts at: 3551 4392 5964 3551
Size: 841 1572 4236
Fragments arranged by size:
4236 1572 841
ApaI G_GGCC'C
Cuts at: 2086 2086
Size: 6649
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 2532 3256 3865 3958 4184 4336 6445 1493
Size: 1039 724 609 93 226 152 2109 1697
Fragments arranged by size:
2109 1697 1039 724 609 226 152 93
AseI AT'TA_AT
Cuts at: 160 1032 3729 5203 160
Size: 872 2697 1474 1606
Fragments arranged by size:
2697 1606 1474 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 3974 1870
Size: 73 2031 4545
Fragments arranged by size:
4545 2031 73
AvrII C'CTAG_G
Cuts at: 2069 2069
Size: 6649
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3156 3189 3156
Size: 33 6616
BamHI G'GATC_C
Cuts at: 3567 3979 4346 3567
Size: 412 367 5870
Fragments arranged by size:
5870 412 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 3970 5425 618
Size: 325 217 627 807 1376 1455 1842
Fragments arranged by size:
1842 1455 1376 807 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2086 2155 2481 3054 3099 3968 1391
Size: 695 69 326 573 45 869 4072
Fragments arranged by size:
4072 869 695 573 326 69 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3599 928
Size: 971 1700 3978
Fragments arranged by size:
3978 1700 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3668 3702 4840 4874 1903
Size: 34 1731 34 1138 34 3678
Fragments arranged by size:
3678 1731 1138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2901 4541 5751 2901
Size: 1640 1210 3799
Fragments arranged by size:
3799 1640 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5260 136
Size: 107 122 71 631 605 3588 1525
Fragments arranged by size:
3588 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6644 6644
Size: 6649
BlpI GC'TnA_GC
Cuts at: 2957 3135 4066 2957
Size: 178 931 5540
Fragments arranged by size:
5540 931 178
Bme1580I G_kGCm'C
Cuts at: 1790 2086 3007 4710 5866 1790
Size: 296 921 1703 1156 2573
Fragments arranged by size:
2573 1703 1156 921 296
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6649
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3481 3539 4380
Size: 416 53 83 399 24 2064 58 841
Cuts at: 4380 5338 442
Size: 958 1753
Fragments arranged by size:
2064 1753 958 841 416 399 83 58
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3444 5309 1239
Size: 2205 1865 2579
Fragments arranged by size:
2579 2205 1865
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2338 2638 4774 5639 5937 2338
Size: 300 2136 865 298 3050
Fragments arranged by size:
3050 2136 865 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3470 5312 882
Size: 617 1971 1842 2219
Fragments arranged by size:
2219 1971 1842 617
BsaAI yAC'GTr
Cuts at: 493 1204 3946 4440 493
Size: 711 2742 494 2702
Fragments arranged by size:
2742 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6643 6643
Size: 6649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4456 4838 5473 275
Size: 53 83 186 936 2923 382 635 1451
Fragments arranged by size:
2923 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2233 3082 5082 5480 5754 5901 6312 2233
Size: 849 2000 398 274 147 411 2570
Fragments arranged by size:
2570 2000 849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2439 2468 2469 2498 3352
Size: 30 165 30 1036 29 1 29 854
Cuts at: 3352 3382 1178
Size: 30 4445
Fragments arranged by size:
4445 1036 854 165 30 30 30 29
29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3009 1186
Size: 1823 4826
BseYI C'CCAG_C
Cuts at: 5852 6348 5852
Size: 496 6153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6076
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4099 4860 5888 1540
Size: 275 186 2098 761 1028 2301
Fragments arranged by size:
2301 2098 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 2481 3054 3240 3515 3804 4710 4795 5866
Size: 304 573 186 275 289 906 85 1071
Cuts at: 5866 2177
Size: 2960
Fragments arranged by size:
2960 1071 906 573 304 289 275 186
85
BsmI GAATG_Cn'
Cuts at: 2809 4160 4253 2809
Size: 1351 93 5205
Fragments arranged by size:
5205 1351 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 6649
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2155 2177 2481 3007 3054 3099
Size: 399 296 69 22 304 526 47 45
Cuts at: 3099 3240 3515 3804 3968 4710 4795 5866 1391
Size: 141 275 289 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 304 296 289
275 164 141 85 69 47 45 22
BspEI T'CCGG_A
Cuts at: 2233 6312 2233
Size: 4079 2570
BspHI T'CATG_A
Cuts at: 2872 3201 3588 4538 6268 2872
Size: 329 387 950 1730 3253
Fragments arranged by size:
3253 1730 950 387 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2168 2229 3783 4004 844
Size: 1324 61 1554 221 3489
Fragments arranged by size:
3489 1554 1324 221 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2674 2905 4536 1394
Size: 429 851 231 1631 3507
Fragments arranged by size:
3507 1631 851 429 231
BsrDI GCAATG_nn'
Cuts at: 65 1248 5144 5318 65
Size: 1183 3896 174 1396
Fragments arranged by size:
3896 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5293 5480 1700
Size: 110 3483 187 2869
Fragments arranged by size:
3483 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3291 3925 96
Size: 3195 634 2820
Fragments arranged by size:
3195 2820 634
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 4709 5721 4709
Size: 1012 5637
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6649
BstBI TT'CG_AA
Cuts at: 2007 5453 2007
Size: 3446 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6649
BstXI CCAn_nnnn'nTGG
Cuts at: 3463 3463
Size: 6649
BstYI r'GATC_y
Cuts at: 1972 2695 3567 3979 4346 4731 4748 6200 6286
Size: 723 872 412 367 385 17 1452 86
Cuts at: 6286 6298 6644 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 872 723 412 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2458 2458
Size: 6649
BtgI C'CryG_G
Cuts at: 513 1065 3762 513
Size: 552 2697 3400
Fragments arranged by size:
3400 2697 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5910
Fragments arranged by size:
5910 683 56
BtsI GCAGTG_nn'
Cuts at: 3651 4165 4959 4979 3651
Size: 514 794 20 5321
Fragments arranged by size:
5321 794 514 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2747 2782 529
Size: 35 2183 35 4396
Fragments arranged by size:
4396 2183 35 35
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3113 5656 809
Size: 507 1797 2543 1802
Fragments arranged by size:
2543 1802 1797 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3624 4096 4985
Size: 54 1425 50 275 18 1794 472 889
Cuts at: 4985 6614 8
Size: 1629 43
Fragments arranged by size:
1794 1629 1425 889 472 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2885 4579 1353
Size: 771 761 1694 3423
Fragments arranged by size:
3423 1694 771 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5236 5610 5756 387
Size: 1064 138 3647 374 146 1280
Fragments arranged by size:
3647 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2479 3052 2479
Size: 573 6076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2804 3141 3318 3444 4712 5309
Size: 393 860 312 337 177 126 1268 597
Cuts at: 5309 6096 6364 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1268 860 787 597 393 337 312
268 177 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2225
Size: 6649
EcoO109I rG'GnC_Cy
Cuts at: 2066 2082 2083 3452 2066
Size: 16 1 1369 5263
Fragments arranged by size:
5263 1369 16 1
EcoRI G'AATT_C
Cuts at: 2532 3958 4336 2532
Size: 1426 378 4845
Fragments arranged by size:
4845 1426 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2529 2056
Size: 473 6176
FspI TGC'GCA
Cuts at: 5155 5155
Size: 6649
HaeII r_GCGC'y
Cuts at: 1678 5796 1678
Size: 4118 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2228 2260 3481
Size: 32 135 32 457 32 167 32 1221
Cuts at: 3481 3513 5290 5322 5364 5396 1373
Size: 32 1777 32 42 32 2626
Fragments arranged by size:
2626 1777 1221 457 167 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3469 3522 3856 3993 4239 669
Size: 988 281 1531 53 334 137 246 3079
Fragments arranged by size:
3079 1531 988 334 281 246 137 53
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6649
HpaI GTT'AAC
Cuts at: 3856 4239 3856
Size: 383 6266
KpnI G_GTAC'C
Cuts at: 3974 3974
Size: 6649
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2637 5763 5947 1305
Size: 1332 3126 184 2007
Fragments arranged by size:
3126 2007 1332 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3626 10
Size: 54 1425 343 1794 3033
Fragments arranged by size:
3033 1794 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 3299 3461 3485 3545 3635 3761
Size: 648 1131 1002 162 24 60 90 126
Cuts at: 3761 4607 4966 5125 518
Size: 846 359 159 2042
Fragments arranged by size:
2042 1131 1002 846 648 359 162 159
126 90 60 24
MspA1I CmG'CkG
Cuts at: 1606 2961 3134 4065 4742 5890 6135 1606
Size: 1355 173 931 677 1148 245 2120
Fragments arranged by size:
2120 1355 1148 931 677 245 173
NcoI C'CATG_G
Cuts at: 513 1065 3762 513
Size: 552 2697 3400
Fragments arranged by size:
3400 2697 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 3075 3292 3300 4007 5552 1132
Size: 97 1846 217 8 707 1545 2229
Fragments arranged by size:
2229 1846 1545 707 217 97 8
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6649
PciI A'CATG_T
Cuts at: 3296 5548 3296
Size: 2252 4397
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6649
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6231 6263 4718
Size: 32 1481 32 5104
Fragments arranged by size:
5104 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 3452 2066
Size: 1386 5263
PshAI GACnn'nnGTC
Cuts at: 1613 2424 1613
Size: 811 5838
PsiI TTA'TAA
Cuts at: 4219 4219
Size: 6649
PspOMI G'GGCC_C
Cuts at: 2082 2082
Size: 6649
PstI C_TGCA'G
Cuts at: 830 2183 3106 3358 3646 4001 830
Size: 1353 923 252 288 355 3478
Fragments arranged by size:
3478 1353 923 355 288 252
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
PvuII CAG'CTG
Cuts at: 2961 3134 2961
Size: 173 6476
SacI G_AGCT'C
Cuts at: 2481 3054 2481
Size: 573 6076
SalI G'TCGA_C
Cuts at: 1936 3467 3520 3991 1936
Size: 1531 53 471 4594
Fragments arranged by size:
4594 1531 471 53
SanDI GG'GwC_CC
Cuts at: 3452 3452
Size: 6649
SapI GCTCTTCn'nnn_
Cuts at: 2124 2124
Size: 6649
SbfI CC_TGCA'GG
Cuts at: 4001 4001
Size: 6649
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SfcI C'TryA_G
Cuts at: 826 1045 2179 2336 3102 3354 3642 3997 5132
Size: 219 1134 157 766 252 288 355 1135
Cuts at: 5132 5813 6004 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1134 766 681 355 288 252
219 191 157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 3976 1872
Size: 2104 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2317 2617 4753 5654 5916 820
Size: 197 926 374 300 2136 901 262 1553
Fragments arranged by size:
2136 1553 926 901 374 300 262 197
SnaBI TAC'GTA
Cuts at: 493 3946 4440 493
Size: 3453 494 2702
Fragments arranged by size:
3453 2702 494
SpeI A'CTAG_T
Cuts at: 152 2827 152
Size: 2675 3974
SphI G_CATG'C
Cuts at: 1132 4007 1132
Size: 2875 3774
SspI AAT'ATT
Cuts at: 5 52 4573 5
Size: 47 4521 2081
Fragments arranged by size:
4521 2081 47
StuI AGG'CCT
Cuts at: 1517 2728 1517
Size: 1211 5438
StyI C'CwwG_G
Cuts at: 513 1065 2069 2927 3762 4088 513
Size: 552 1004 858 835 326 3074
Fragments arranged by size:
3074 1004 858 835 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3603 3775 4676 4693 4846 5031 5476
Size: 1255 2023 172 901 17 153 185 445
Cuts at: 5476 325
Size: 1498
Fragments arranged by size:
2023 1498 1255 901 445 185 172 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3291 3925 4895
Size: 274 80 33 51 1443 1314 634 970
Cuts at: 4895 6607 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1314 970 634 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6609 6641 6609
Size: 32 6617
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6649
XbaI T'CTAG_A
Cuts at: 2899 3985 2899
Size: 1086 5563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3468 3634 3468
Size: 166 6483
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 3974 1870
Size: 2104 4545
XmnI GAAnn'nnTTC
Cuts at: 2306 2536 3065 3533 4778 2306
Size: 230 529 468 1245 4177
Fragments arranged by size:
4177 1245 529 468 230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4457 276
Size: 53 83 186 936 2923 2468
Fragments arranged by size:
2923 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI