Restriction map for
pFN21ASDA1561
(Circular) (Six-base) MAPSORT of: pFN21ASDA1561.seq Check: 8792 from: 1 to: 8977
pFN21ASDA1561 8977 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2177 6787 278
Size: 53 83 186 936 641 4610 2468
Fragments arranged by size:
4610 2468 936 641 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2517 3259 6320 1937
Size: 580 742 3061 4594
Fragments arranged by size:
4594 3061 742 580
Acc65I G'GTAC_C
Cuts at: 6298 6298
Size: 8977
AclI AA'CG_TT
Cuts at: 1563 7104 7477 1563
Size: 5541 373 3063
Fragments arranged by size:
5541 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3086 3425 3668 3675 5126 5447 5618 7040
Size: 1454 339 243 7 1451 321 171 1422
Cuts at: 7040 8424 8692 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1454 1451 1422 1384 339 321 268
243 171 7
AflII C'TTAA_G
Cuts at: 820 1017 3109 4396 4490 4511 820
Size: 197 2092 1287 94 21 5286
Fragments arranged by size:
5286 2092 1287 197 94 21
AflIII A'CryG_T
Cuts at: 1203 4644 5353 7876 8853 1203
Size: 3441 709 2523 977 1327
Fragments arranged by size:
3441 2523 1327 977 709
AgeI A'CCGG_T
Cuts at: 7808 7808
Size: 8977
AhdI GACnn_n'nnGTC
Cuts at: 7706 7706
Size: 8977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2492 1184
Size: 1308 7669
AlwNI CAG_nnn'CTG
Cuts at: 6720 8292 6720
Size: 1572 7405
ApaLI G'TGCA_C
Cuts at: 7034 8190 7034
Size: 1156 7821
ApoI r'AATT_y
Cuts at: 1493 2848 2958 3342 3914 4688 4709 5012 6286
Size: 1355 110 384 572 774 21 303 1274
Cuts at: 6286 6512 6664 8773 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 1274 774 572 384 303
226 152 110 21
AseI AT'TA_AT
Cuts at: 160 1032 7531 160
Size: 872 6499 1606
Fragments arranged by size:
6499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8977
AvaI C'yCGr_G
Cuts at: 1870 1943 6302 1870
Size: 73 4359 4545
Fragments arranged by size:
4545 4359 73
BamHI G'GATC_C
Cuts at: 6307 6674 6307
Size: 367 8610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 6298 7753 618
Size: 325 217 627 294 4217 1455 1842
Fragments arranged by size:
4217 1842 1455 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 3935 4823 4944 6296 1391
Size: 2544 888 121 1352 4072
Fragments arranged by size:
4072 2544 1352 888 121
BbeI G_GCGC'C
Cuts at: 2085 2085
Size: 8977
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 2786 4281 4536 928
Size: 971 328 559 1495 255 5369
Fragments arranged by size:
5369 1495 971 559 328 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7168 7202 1903
Size: 34 5231 34 3678
Fragments arranged by size:
5231 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6869 8079 6869
Size: 1210 7767
BclI T'GATC_A
Cuts at: 1548 3157 1548
Size: 1609 7368
BfrBI ATG'CAT
Cuts at: 2363 3515 4962 2363
Size: 1152 1447 6378
Fragments arranged by size:
6378 1447 1152
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2450 7588 136
Size: 107 122 71 631 605 778 5138 1525
Fragments arranged by size:
5138 1525 778 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2228 3281 8972 2228
Size: 1053 5691 2233
Fragments arranged by size:
5691 2233 1053
BlpI GC'TnA_GC
Cuts at: 4059 6394 4059
Size: 2335 6642
Bme1580I G_kGCm'C
Cuts at: 1790 2064 7038 8194 1790
Size: 274 4974 1156 2573
Fragments arranged by size:
4974 2573 1156 274
BmgBI CAC'GTC
Cuts at: 1318 2059 4066 1318
Size: 741 2007 6229
Fragments arranged by size:
6229 2007 741
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 6708 7666
Size: 416 53 83 399 24 1892 3399 958
Cuts at: 7666 442
Size: 1753
Fragments arranged by size:
3399 1892 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2349 2349
Size: 8977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6140 6221 7637 1239
Size: 4901 81 1416 2579
Fragments arranged by size:
4901 2579 1416 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 2285 4055 4193 5602 7102 7967 8265 2018
Size: 267 1770 138 1409 1500 865 298 2730
Fragments arranged by size:
2730 1770 1500 1409 865 298 267 138
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7640 882
Size: 617 6141 2219
Fragments arranged by size:
6141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6274 6768 493
Size: 711 5070 494 2702
Fragments arranged by size:
5070 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4701 8971 4701
Size: 4270 4707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2174 6784 7166
Size: 53 83 186 936 549 92 4610 382
Cuts at: 7166 7801 275
Size: 635 1451
Fragments arranged by size:
4610 1451 936 635 549 382 186 92
83 53
BsaWI w'CCGG_w
Cuts at: 7410 7808 8082 8229 8640 7410
Size: 398 274 147 411 7747
Fragments arranged by size:
7747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2583 2613 3393 3423 1178
Size: 30 165 30 1180 30 780 30 6732
Fragments arranged by size:
6732 1180 780 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2604 4196 5012 1186
Size: 876 542 1592 816 5151
Fragments arranged by size:
5151 1592 876 816 542
BseYI C'CCAG_C
Cuts at: 8180 8676 8180
Size: 496 8481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2415 3548 4868 6180 1163
Size: 573 679 1133 1320 1312 3960
Fragments arranged by size:
3960 1320 1312 1133 679 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6427 7188 8216 1540
Size: 275 186 4426 761 1028 2301
Fragments arranged by size:
4426 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3935 4806 4823 4944 7038 7123 8194 3935
Size: 871 17 121 2094 85 1071 4718
Fragments arranged by size:
4718 2094 1071 871 121 85 17
BsmI GAATG_Cn'
Cuts at: 2281 2509 3446 4964 6488 6581 2281
Size: 228 937 1518 1524 93 4677
Fragments arranged by size:
4677 1524 1518 937 228 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3935 4806 4823 4944 6296 7038
Size: 399 274 1871 871 17 121 1352 742
Cuts at: 7038 7123 8194 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1871 1352 1071 871 742 399 274
121 85 17
BspEI T'CCGG_A
Cuts at: 8640 8640
Size: 8977
BspHI T'CATG_A
Cuts at: 5185 6866 8596 5185
Size: 1681 1730 5566
Fragments arranged by size:
5566 1730 1681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 6332 844
Size: 1580 3908 3489
Fragments arranged by size:
3908 3489 1580
BsrBI CCG'CTC
Cuts at: 1394 1823 2594 6864 1394
Size: 429 771 4270 3507
Fragments arranged by size:
4270 3507 771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2454 3372 7472 7646 65
Size: 1183 1206 918 4100 174 1396
Fragments arranged by size:
4100 1396 1206 1183 918 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7621 7808 1700
Size: 110 5811 187 2869
Fragments arranged by size:
5811 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8977
BssHII G'CGCG_C
Cuts at: 1925 2097 1925
Size: 172 8805
BssSI C'ACGA_G
Cuts at: 4805 7037 8049 4805
Size: 2232 1012 5733
Fragments arranged by size:
5733 2232 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 794
Size: 1265 7712
BstBI TT'CG_AA
Cuts at: 2007 7781 2007
Size: 5774 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8977
BstYI r'GATC_y
Cuts at: 1972 2228 3281 6307 6674 7059 7076 8528 8614
Size: 256 1053 3026 367 385 17 1452 86
Cuts at: 8614 8626 8972 1972
Size: 12 346 1977
Fragments arranged by size:
3026 1977 1452 1053 385 367 346 256
86 17 12
BstZ17I GTA'TAC
Cuts at: 3260 3260
Size: 8977
Bsu36I CC'TnA_GG
Cuts at: 2045 3619 2045
Size: 1574 7403
BtgI C'CryG_G
Cuts at: 513 1065 2488 513
Size: 552 1423 7002
Fragments arranged by size:
7002 1423 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8238
Fragments arranged by size:
8238 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 4907 5334 6493 7287 7307 2316
Size: 2591 427 1159 794 20 3986
Fragments arranged by size:
3986 2591 1159 794 427 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8942
DraI TTT'AAA
Cuts at: 3142 3348 4263 5017 6281 3142
Size: 206 915 754 1264 5838
Fragments arranged by size:
5838 1264 915 754 206
DraIII CAC_nnn'GTG
Cuts at: 3433 4007 6109 3433
Size: 574 2102 6301
Fragments arranged by size:
6301 2102 574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7984 809
Size: 507 6668 1802
Fragments arranged by size:
6668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2719 4872 5763
Size: 54 1425 50 275 18 889 2153 891
Cuts at: 5763 6132 6424 7313 8942 8
Size: 369 292 889 1629 43
Fragments arranged by size:
2153 1629 1425 891 889 889 369 292
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6424 1537
Size: 275 4612 4090
Fragments arranged by size:
4612 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2340 3527 3741 3923 4614 4890 5423
Size: 668 319 1187 214 182 691 276 533
Cuts at: 5423 5512 6907 1353
Size: 89 1395 3423
Fragments arranged by size:
3423 1395 1187 691 668 533 319 276
214 182 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2539 7564 7938 8084 387
Size: 1064 138 950 5025 374 146 1280
Fragments arranged by size:
5025 1280 1064 950 374 146 138
EcoICRI GAG'CTC
Cuts at: 3933 4821 4942 3933
Size: 888 121 7968
Fragments arranged by size:
7968 888 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3086 3425 3668 3675 5126 5447 5618
Size: 393 1454 339 243 7 1451 321 171
Cuts at: 5618 6140 6221 7040 7637 8424 8692 1239
Size: 522 81 819 597 787 268 1524
Fragments arranged by size:
1524 1454 1451 819 787 597 522 393
339 321 268 243 171 81 7
EcoO109I rG'GnC_Cy
Cuts at: 2493 2493
Size: 8977
EcoRI G'AATT_C
Cuts at: 6286 6664 6286
Size: 378 8599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3221 3221
Size: 8977
FspI TGC'GCA
Cuts at: 7483 7483
Size: 8977
HaeII r_GCGC'y
Cuts at: 1678 2085 8124 1678
Size: 407 6039 2531
Fragments arranged by size:
6039 2531 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2884 2916 3393 3425 4450
Size: 32 135 32 1312 32 477 32 1025
Cuts at: 4450 4482 4576 4608 5033 5065 6079 6111 7618
Size: 32 94 32 425 32 1014 32 1507
Cuts at: 7618 7650 7692 7724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1507 1312 1025 1014 477 425 135
94 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2573 4146 5695 6321 6567 669
Size: 988 281 635 1573 1549 626 246 3079
Fragments arranged by size:
3079 1573 1549 988 635 626 281 246
HindIII A'AGCT_T
Cuts at: 748 2701 3106 3447 4052 4322 748
Size: 1953 405 341 605 270 5403
Fragments arranged by size:
5403 1953 605 405 341 270
HpaI GTT'AAC
Cuts at: 4146 6567 4146
Size: 2421 6556
KasI G'GCGC_C
Cuts at: 2081 2081
Size: 8977
KpnI G_GTAC'C
Cuts at: 6302 6302
Size: 8977
MfeI C'AATT_G
Cuts at: 2392 4233 4983 5403 5483 6576 2392
Size: 1841 750 420 80 1093 4793
Fragments arranged by size:
4793 1841 1093 750 420 80
MluI A'CGCG_T
Cuts at: 8853 8853
Size: 8977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4341 5469 5542 5619 8091 8275 1305
Size: 3036 1128 73 77 2472 184 2007
Fragments arranged by size:
3036 2472 2007 1128 184 77 73
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2721 4874 5765 10
Size: 54 1425 343 889 2153 891 3222
Fragments arranged by size:
3222 2153 1425 891 889 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3460 3854 6210 6248 6935 7294 7453
Size: 648 2294 394 2356 38 687 359 159
Cuts at: 7453 518
Size: 2042
Fragments arranged by size:
2356 2294 2042 687 648 394 359 159
38
MspA1I CmG'CkG
Cuts at: 1606 2476 3013 3908 4501 6085 6130 6393 7070
Size: 870 537 895 593 1584 45 263 677
Cuts at: 7070 8218 8463 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1584 1148 895 870 677 593 537
263 245 45
NarI GG'CG_CC
Cuts at: 2082 2082
Size: 8977
NcoI C'CATG_G
Cuts at: 513 1065 2488 513
Size: 552 1423 7002
Fragments arranged by size:
7002 1423 552
NdeI CA'TA_TG
Cuts at: 387 3270 387
Size: 2883 6094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8977
NotI GC'GGCC_GC
Cuts at: 6424 6424
Size: 8977
NsiI A_TGCA'T
Cuts at: 2365 3517 4964 2365
Size: 1152 1447 6378
Fragments arranged by size:
6378 1447 1152
NspI r_CATG'y
Cuts at: 1132 1229 2105 2363 2411 2863 4916 5357 5813
Size: 97 876 258 48 452 2053 441 456
Cuts at: 5813 6335 7880 1132
Size: 522 1545 2229
Fragments arranged by size:
2229 2053 1545 876 522 456 452 441
258 97 48
PacI TTA_AT'TAA
Cuts at: 2682 2682
Size: 8977
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8977
PciI A'CATG_T
Cuts at: 5353 7876 5353
Size: 2523 6454
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8977
PmeI GTTT'AAAC
Cuts at: 6281 6281
Size: 8977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7046 7078 8559 8591 7046
Size: 32 1481 32 7432
Fragments arranged by size:
7432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8977
PsiI TTA'TAA
Cuts at: 6547 6547
Size: 8977
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2961 2993 3294 3326 2961
Size: 32 301 32 8612
Fragments arranged by size:
8612 301 32 32
PstI C_TGCA'G
Cuts at: 830 2323 2421 6102 6329 830
Size: 1493 98 3681 227 3478
Fragments arranged by size:
3681 3478 1493 227 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8977
PvuII CAG'CTG
Cuts at: 2476 3013 3908 4501 6085 6130 2476
Size: 537 895 593 1584 45 5323
Fragments arranged by size:
5323 1584 895 593 537 45
SacI G_AGCT'C
Cuts at: 3935 4823 4944 3935
Size: 888 121 7968
Fragments arranged by size:
7968 888 121
SalI G'TCGA_C
Cuts at: 1936 6319 1936
Size: 4383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 2340 3923 2021
Size: 319 1583 7075
Fragments arranged by size:
7075 1583 319
SbfI CC_TGCA'GG
Cuts at: 2421 6329 2421
Size: 3908 5069
ScaI AGT'ACT
Cuts at: 7225 8937 7225
Size: 1712 7265
SfcI C'TryA_G
Cuts at: 826 1045 2319 2384 2417 4879 6098 6325 7460
Size: 219 1274 65 33 2462 1219 227 1135
Cuts at: 7460 8141 8332 826
Size: 681 191 1471
Fragments arranged by size:
2462 1471 1274 1219 1135 681 227 219
191 65 33
SfoI GGC'GCC
Cuts at: 2083 2083
Size: 8977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8977
SmaI CCC'GGG
Cuts at: 1872 6304 1872
Size: 4432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 2264 3109 4034 4208 4396
Size: 197 926 90 231 845 925 174 188
Cuts at: 4396 4490 4511 5581 7081 7982 8244 820
Size: 94 21 1070 1500 901 262 1553
Fragments arranged by size:
1553 1500 1070 926 925 901 845 262
231 197 188 174 94 90 21
SnaBI TAC'GTA
Cuts at: 493 6274 6768 493
Size: 5781 494 2702
Fragments arranged by size:
5781 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8977
SphI G_CATG'C
Cuts at: 1132 2105 2411 4916 5813 6335 1132
Size: 973 306 2505 897 522 3774
Fragments arranged by size:
3774 2505 973 897 522 306
SspI AAT'ATT
Cuts at: 5 52 2908 3146 4480 4740 6901 5
Size: 47 2856 238 1334 260 2161 2081
Fragments arranged by size:
2856 2161 2081 1334 260 238 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8977
StyI C'CwwG_G
Cuts at: 513 1065 2488 6416 513
Size: 552 1423 3928 3074
Fragments arranged by size:
3928 3074 1423 552
SwaI ATTT'AAAT
Cuts at: 3142 3142
Size: 8977
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 3414 7004 7021 7174 7359 7804
Size: 1255 565 1269 3590 17 153 185 445
Cuts at: 7804 325
Size: 1498
Fragments arranged by size:
3590 1498 1269 1255 565 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 3245 4785
Size: 274 80 33 51 1443 411 857 1540
Cuts at: 4785 7223 8935 96
Size: 2438 1712 138
Fragments arranged by size:
2438 1712 1540 1443 857 411 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8937 8969 8937
Size: 32 8945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8977
XbaI T'CTAG_A
Cuts at: 6313 6313
Size: 8977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8977
XmaI C'CCGG_G
Cuts at: 1870 6302 1870
Size: 4432 4545
XmnI GAAnn'nnTTC
Cuts at: 6219 7106 6219
Size: 887 8090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2175 6785 276
Size: 53 83 186 936 641 4610 2468
Fragments arranged by size:
4610 2468 936 641 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
Bpu10I BsiWI BsmBI BstXI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SrfI XcmI