Restriction map for
pFN21ASDA1540
(Circular) (Six-base) MAPSORT of: pFN21ASDA1540.seq Check: 9329 from: 1 to: 5998
pFN21ASDA1540 5998 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3044 3044
Size: 5998
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3061 3148 4061 5445 5713 1632
Size: 1429 87 913 1384 268 1917
Fragments arranged by size:
1917 1429 1384 913 268 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2880 1184
Size: 1696 4302
AlwNI CAG_nnn'CTG
Cuts at: 3036 3741 5313 3036
Size: 705 1572 3721
Fragments arranged by size:
3721 1572 705
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3319 4774 618
Size: 325 217 627 1532 1455 1842
Fragments arranged by size:
1842 1532 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 3205 3317 1391
Size: 688 1126 112 4072
Fragments arranged by size:
4072 1126 688 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 2894 3257 928
Size: 971 364 631 363 3669
Fragments arranged by size:
3669 971 631 364 363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 2379 1548
Size: 831 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2130 4059 5215 1790
Size: 340 1929 1156 2573
Fragments arranged by size:
2573 1929 1156 340
BmgBI CAC'GTC
Cuts at: 1318 2491 1318
Size: 1173 4825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2467 3146 3729
Size: 416 53 83 399 24 1050 679 583
Cuts at: 3729 4687 442
Size: 958 1753
Fragments arranged by size:
1753 1050 958 679 583 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3224 4658 1239
Size: 1985 1434 2579
Fragments arranged by size:
2579 1985 1434
Bpu10I CC'TnA_GC
Cuts at: 2709 2709
Size: 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3295 3789 493
Size: 711 2091 494 2702
Fragments arranged by size:
2702 2091 711 494
BsaBI GATnn'nnATC
Cuts at: 2567 5992 2567
Size: 3425 2573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 1178
Size: 30 165 30 750 30 4993
Fragments arranged by size:
4993 750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2768 2951 1186
Size: 856 726 183 4233
Fragments arranged by size:
4233 856 726 183
BseYI C'CCAG_C
Cuts at: 5201 5697 5201
Size: 496 5502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2471 1163
Size: 573 735 4690
Fragments arranged by size:
4690 735 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4059 4144 5215 4059
Size: 85 1071 4842
Fragments arranged by size:
4842 1071 85
BsmI GAATG_Cn'
Cuts at: 2331 3509 3602 2331
Size: 1178 93 4727
Fragments arranged by size:
4727 1178 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2497 2497
Size: 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2130 3205 3317 4059 4144 5215
Size: 399 289 51 1075 112 742 85 1071
Cuts at: 5215 1391
Size: 2174
Fragments arranged by size:
2174 1075 1071 742 399 289 112 85
51
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 3353 844
Size: 2200 309 3489
Fragments arranged by size:
3489 2200 309
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2514 4493 4667 65
Size: 1183 1266 1979 174 1396
Fragments arranged by size:
1979 1396 1266 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 2081 2802 4058 5070 2081
Size: 721 1256 1012 3009
Fragments arranged by size:
3009 1256 1012 721
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3036 794
Size: 2242 3756
BstBI TT'CG_AA
Cuts at: 2007 4802 2007
Size: 2795 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 2521
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2562 3328 3695 4080 4097 5549 5635 5647
Size: 590 766 367 385 17 1452 86 12
Cuts at: 5647 5993 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 766 590 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3129 3129
Size: 5998
BtgI C'CryG_G
Cuts at: 513 1065 2022 2226 2607 513
Size: 552 957 204 381 3904
Fragments arranged by size:
3904 957 552 381 204
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2575 2856 487
Size: 683 56 1349 281 3629
Fragments arranged by size:
3629 1349 683 281 56
BtsI GCAGTG_nn'
Cuts at: 2483 3514 4308 4328 2483
Size: 1031 794 20 4153
Fragments arranged by size:
4153 1031 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3259 5005 809
Size: 507 1943 1746 1802
Fragments arranged by size:
1943 1802 1746 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2334 2745
Size: 54 1425 50 275 18 291 213 411
Cuts at: 2745 2808 3193 3445 4334 5963 8
Size: 63 385 252 889 1629 43
Fragments arranged by size:
1629 1425 889 411 385 291 275 252
213 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2351 2729 3928 1353
Size: 998 378 1199 3423
Fragments arranged by size:
3423 1199 998 378
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3061 3148 3224 4061 4658 5445 5713
Size: 393 1429 87 76 837 597 787 268
Cuts at: 5713 1239
Size: 1524
Fragments arranged by size:
1524 1429 837 787 597 393 268 87
76
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 2203 2211 5145 1678
Size: 525 8 2934 2531
Fragments arranged by size:
2934 2531 525 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3094 3126 3248 3280 4639
Size: 32 135 32 1522 32 122 32 1359
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1522 1359 135 122 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2356 3342 3588 669
Size: 988 281 418 986 246 3079
Fragments arranged by size:
3079 988 986 418 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 5112 5296 1305
Size: 891 2916 184 2007
Fragments arranged by size:
2916 2007 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 2810 3195 10
Size: 54 1425 343 915 63 385 2813
Fragments arranged by size:
2813 1425 915 385 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 2789 2897 3956 4315 4474 518
Size: 648 1353 270 108 1059 359 159 2042
Fragments arranged by size:
2042 1353 1059 648 359 270 159 108
MspA1I CmG'CkG
Cuts at: 1606 2717 3414 4091 5239 5484 1606
Size: 1111 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 1111 697 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2607 513
Size: 552 957 585 3904
Fragments arranged by size:
3904 957 585 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2452 2636 3109 3356 4901 1132
Size: 97 1223 184 473 247 1545 2229
Fragments arranged by size:
2229 1545 1223 473 247 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PstI C_TGCA'G
Cuts at: 830 3350 830
Size: 2520 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2717 2717
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 2477 2861 4246 5958 2477
Size: 384 1385 1712 2517
Fragments arranged by size:
2517 1712 1385 384
SexAI A'CCwGG_T
Cuts at: 2532 2532
Size: 5998
SfcI C'TryA_G
Cuts at: 826 1045 3346 4481 5162 5353 826
Size: 219 2301 1135 681 191 1471
Fragments arranged by size:
2301 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4102 5003 5265 820
Size: 197 926 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3295 3789 493
Size: 2802 494 2702
Fragments arranged by size:
2802 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2022 2058 2607 3437 513
Size: 552 957 36 549 830 3074
Fragments arranged by size:
3074 957 830 552 549 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4025 4042 4195 4380 4825 325
Size: 1255 2445 17 153 185 445 1498
Fragments arranged by size:
2445 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 2859 4244
Size: 274 80 33 51 1443 498 384 1385
Cuts at: 4244 5956 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1385 498 384 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5958 5990 5958
Size: 32 5966
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3242 3242
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI