Restriction map for
pFN21ASDA1537
(Circular) (Six-base) MAPSORT of: pFN21ASDA1537.seq Check: 5414 from: 1 to: 6499
pFN21ASDA1537 6499 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4309 278
Size: 53 83 186 936 2773 2468
Fragments arranged by size:
2773 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3842 1937
Size: 1905 4594
Acc65I G'GTAC_C
Cuts at: 3820 3820
Size: 6499
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 3108 3234 4562 5946 6214 1632
Size: 656 820 126 1328 1384 268 1917
Fragments arranged by size:
1917 1384 1328 820 656 268 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6302
AflIII A'CryG_T
Cuts at: 1203 5398 6375 1203
Size: 4195 977 1327
Fragments arranged by size:
4195 1327 977
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 5228 5228
Size: 6499
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6499
AlwNI CAG_nnn'CTG
Cuts at: 3577 4242 5814 3577
Size: 665 1572 4262
Fragments arranged by size:
4262 1572 665
ApaI G_GGCC'C
Cuts at: 2195 2195
Size: 6499
ApaLI G'TGCA_C
Cuts at: 4556 5712 4556
Size: 1156 5343
ApoI r'AATT_y
Cuts at: 1493 3629 3675 3808 4034 4186 6295 1493
Size: 2136 46 133 226 152 2109 1697
Fragments arranged by size:
2136 2109 1697 226 152 133 46
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 3824 1870
Size: 73 1881 4545
Fragments arranged by size:
4545 1881 73
BamHI G'GATC_C
Cuts at: 3829 4196 3829
Size: 367 6132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3820 5275 618
Size: 325 217 627 2033 1455 1842
Fragments arranged by size:
2033 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2195 3818 1391
Size: 804 1623 4072
Fragments arranged by size:
4072 1623 804
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 3549 928
Size: 971 377 1273 3878
Fragments arranged by size:
3878 1273 971 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4690 4724 1903
Size: 34 2753 34 3678
Fragments arranged by size:
3678 2753 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4391 5601 4391
Size: 1210 5289
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6499
BfrBI ATG'CAT
Cuts at: 3758 3758
Size: 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5110 136
Size: 107 122 71 631 605 3438 1525
Fragments arranged by size:
3438 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3358 6494 3358
Size: 3136 3363
BlpI GC'TnA_GC
Cuts at: 3518 3916 3518
Size: 398 6101
Bme1580I G_kGCm'C
Cuts at: 1790 2195 4560 5716 1790
Size: 405 2365 1156 2573
Fragments arranged by size:
2573 2365 1156 405
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4230 5188 442
Size: 416 53 83 399 24 2813 958 1753
Fragments arranged by size:
2813 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2933 1056
Size: 1877 4622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2711 3002 3428 5159 1239
Size: 1472 291 426 1731 2579
Fragments arranged by size:
2579 1731 1472 426 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2389 4624 5489 5787 2389
Size: 2235 865 298 3101
Fragments arranged by size:
3101 2235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5162 882
Size: 617 3663 2219
Fragments arranged by size:
3663 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3796 4290 493
Size: 711 2592 494 2702
Fragments arranged by size:
2702 2592 711 494
BsaBI GATnn'nnATC
Cuts at: 2460 3453 3778 6493 2460
Size: 993 325 2715 2466
Fragments arranged by size:
2715 2466 993 325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4306 4688 5323 275
Size: 53 83 186 936 2773 382 635 1451
Fragments arranged by size:
2773 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4932 5330 5604 5751 6162 4932
Size: 398 274 147 411 5269
Fragments arranged by size:
5269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 2232 2262 1178
Size: 30 165 30 798 30 1 30 5415
Fragments arranged by size:
5415 798 165 30 30 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6499
BseYI C'CCAG_C
Cuts at: 5702 6198 5702
Size: 496 6003
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5926
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3949 4710 5738 1540
Size: 275 186 1948 761 1028 2301
Fragments arranged by size:
2301 1948 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4560 4645 5716 4560
Size: 85 1071 5343
Fragments arranged by size:
5343 1071 85
BsmI GAATG_Cn'
Cuts at: 2839 4010 4103 2839
Size: 1171 93 5235
Fragments arranged by size:
5235 1171 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 3818 4560 4645 5716 1391
Size: 399 405 1623 742 85 1071 2174
Fragments arranged by size:
2174 1623 1071 742 405 399 85
BspEI T'CCGG_A
Cuts at: 6162 6162
Size: 6499
BspHI T'CATG_A
Cuts at: 3478 4388 6118 3478
Size: 910 1730 3859
Fragments arranged by size:
3859 1730 910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3854 844
Size: 3010 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4386 1394
Size: 429 2563 3507
Fragments arranged by size:
3507 2563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2128 3566 3758 4994 5168 65
Size: 1183 880 1438 192 1236 174 1396
Fragments arranged by size:
1438 1396 1236 1183 880 192 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5143 5330 1700
Size: 110 3333 187 2869
Fragments arranged by size:
3333 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2865 96
Size: 2769 3730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6499
BssSI C'ACGA_G
Cuts at: 4559 5571 4559
Size: 1012 5487
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3349 794
Size: 2555 3944
BstBI TT'CG_AA
Cuts at: 2007 5303 2007
Size: 3296 3203
BstEII G'GTnAC_C
Cuts at: 1184 2797 1184
Size: 1613 4886
BstYI r'GATC_y
Cuts at: 1972 2254 2362 3358 3829 4196 4581 4598 6050
Size: 282 108 996 471 367 385 17 1452
Cuts at: 6050 6136 6148 6494 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 996 471 385 367 346 282
108 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4990
Fragments arranged by size:
4990 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5760
Fragments arranged by size:
5760 683 56
BtsI GCAGTG_nn'
Cuts at: 4015 4809 4829 4015
Size: 794 20 5685
Fragments arranged by size:
5685 794 20
ClaI AT'CG_AT
Cuts at: 1554 2454 1554
Size: 900 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6464
DraI TTT'AAA
Cuts at: 2526 3803 2526
Size: 1277 5222
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 2328 3946
Size: 54 1425 50 275 18 241 257 1618
Cuts at: 3946 4835 6464 8
Size: 889 1629 43
Fragments arranged by size:
1629 1618 1425 889 275 257 241 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3946 1537
Size: 275 2134 4090
Fragments arranged by size:
4090 2134 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 3501 4429 1353
Size: 1332 816 928 3423
Fragments arranged by size:
3423 1332 928 816
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5086 5460 5606 387
Size: 1064 138 3497 374 146 1280
Fragments arranged by size:
3497 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2711 3002 3108 3234 3428 4562
Size: 393 656 423 291 106 126 194 1134
Cuts at: 4562 5159 5946 6214 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1134 787 656 597 423 393 291
268 194 126 106
EcoO109I rG'GnC_Cy
Cuts at: 2191 2192 2191
Size: 1 6498
EcoRI G'AATT_C
Cuts at: 3629 3808 4186 3629
Size: 179 378 5942
Fragments arranged by size:
5942 378 179
EcoRV GAT'ATC
Cuts at: 2992 2992
Size: 6499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3452 3452
Size: 6499
FspI TGC'GCA
Cuts at: 5005 5005
Size: 6499
HaeII r_GCGC'y
Cuts at: 1678 5646 1678
Size: 3968 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2295 2327 2474
Size: 32 135 32 660 32 31 32 147
Cuts at: 2474 2506 3368 3400 5140 5172 5214 5246 1373
Size: 32 862 32 1740 32 42 32 2626
Fragments arranged by size:
2626 1740 862 660 147 135 42 32
32 32 32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 3843 4089 669
Size: 988 281 1905 246 3079
Fragments arranged by size:
3079 1905 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3515 748
Size: 2767 3732
HpaI GTT'AAC
Cuts at: 4089 4089
Size: 6499
KpnI G_GTAC'C
Cuts at: 3824 3824
Size: 6499
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 6375 6375
Size: 6499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2479 5613 5797 1305
Size: 1174 3134 184 2007
Fragments arranged by size:
3134 2007 1174 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4677
Fragments arranged by size:
4677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3062 4457 4816 4975 518
Size: 648 1896 1395 359 159 2042
Fragments arranged by size:
2042 1896 1395 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3169 3298 3915 4592 5740 5985 1606
Size: 1563 129 617 677 1148 245 2120
Fragments arranged by size:
2120 1563 1148 677 617 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4990
Fragments arranged by size:
4990 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6499
NheI G'CTAG_C
Cuts at: 1052 2929 1052
Size: 1877 4622
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NsiI A_TGCA'T
Cuts at: 3760 3760
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 3857 5402 1132
Size: 97 2628 1545 2229
Fragments arranged by size:
2628 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6499
PciI A'CATG_T
Cuts at: 5398 5398
Size: 6499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6499
PfoI T'CCnGG_A
Cuts at: 2250 2727 3444 2250
Size: 477 717 5305
Fragments arranged by size:
5305 717 477
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 6081 6113 4568
Size: 32 1481 32 4954
Fragments arranged by size:
4954 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6499
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PspOMI G'GGCC_C
Cuts at: 2191 2191
Size: 6499
PstI C_TGCA'G
Cuts at: 830 3157 3270 3851 830
Size: 2327 113 581 3478
Fragments arranged by size:
3478 2327 581 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 3169 3298 3169
Size: 129 6370
SalI G'TCGA_C
Cuts at: 1936 3841 1936
Size: 1905 4594
SapI GCTCTTCn'nnn_
Cuts at: 3501 3501
Size: 6499
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 4747 6459 4747
Size: 1712 4787
SexAI A'CCwGG_T
Cuts at: 2260 2260
Size: 6499
SfcI C'TryA_G
Cuts at: 826 1045 3153 3266 3658 3847 4982 5663 5854
Size: 219 2108 113 392 189 1135 681 191
Cuts at: 5854 826
Size: 1471
Fragments arranged by size:
2108 1471 1135 681 392 219 191 189
113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 3826 1872
Size: 1954 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2368 4603 5504 5766 820
Size: 197 926 425 2235 901 262 1553
Fragments arranged by size:
2235 1553 926 901 425 262 197
SnaBI TAC'GTA
Cuts at: 493 3796 4290 493
Size: 3303 494 2702
Fragments arranged by size:
3303 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6499
SphI G_CATG'C
Cuts at: 1132 3857 1132
Size: 2725 3774
SspI AAT'ATT
Cuts at: 5 52 4423 5
Size: 47 4371 2081
Fragments arranged by size:
4371 2081 47
StuI AGG'CCT
Cuts at: 1517 3021 1517
Size: 1504 4995
StyI C'CwwG_G
Cuts at: 513 1065 2022 3938 513
Size: 552 957 1916 3074
Fragments arranged by size:
3074 1916 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4526 4543 4696 4881 5326 325
Size: 1255 2946 17 153 185 445 1498
Fragments arranged by size:
2946 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2865 2935 4745
Size: 274 80 33 51 1443 888 70 1810
Cuts at: 4745 6457 96
Size: 1712 138
Fragments arranged by size:
1810 1712 1443 888 274 138 80 70
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6459 6491 6459
Size: 32 6467
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6499
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 2977 3166 2809
Size: 168 189 6142
Fragments arranged by size:
6142 189 168
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6499
XmaI C'CCGG_G
Cuts at: 1870 3824 1870
Size: 1954 4545
XmnI GAAnn'nnTTC
Cuts at: 3360 4628 3360
Size: 1268 5231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4307 276
Size: 53 83 186 936 2773 2468
Fragments arranged by size:
2773 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI