Restriction map for
pFN21ASDA1499
(Circular) (Six-base) MAPSORT of: pFN21ASDA1499.seq Check: 5630 from: 1 to: 6580
pFN21ASDA1499 6580 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3799 3799
Size: 6580
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2297 4390 278
Size: 53 83 186 936 761 2093 2468
Fragments arranged by size:
2468 2093 936 761 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3309 3923 1937
Size: 1372 614 4594
Fragments arranged by size:
4594 1372 614
Acc65I G'GTAC_C
Cuts at: 2990 3174 3901 2990
Size: 184 727 5669
Fragments arranged by size:
5669 727 184
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2847 2922 4643 6027 6295 1632
Size: 1215 75 1721 1384 268 1917
Fragments arranged by size:
1917 1721 1384 1215 268 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 2505 3081 5479 6456 1203
Size: 1302 576 2398 977 1327
Fragments arranged by size:
2398 1327 1302 977 576
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3617 3813 1184
Size: 2433 196 3951
Fragments arranged by size:
3951 2433 196
AlwNI CAG_nnn'CTG
Cuts at: 4323 5895 4323
Size: 1572 5008
ApaI G_GGCC'C
Cuts at: 2277 2277
Size: 6580
ApaLI G'TGCA_C
Cuts at: 2438 4637 5793 2438
Size: 2199 1156 3225
Fragments arranged by size:
3225 2199 1156
ApoI r'AATT_y
Cuts at: 1493 3889 4115 4267 6376 1493
Size: 2396 226 152 2109 1697
Fragments arranged by size:
2396 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 2260 2486 3905 1870
Size: 73 317 226 1419 4545
Fragments arranged by size:
4545 1419 317 226 73
BamHI G'GATC_C
Cuts at: 2890 3113 3397 3910 4277 2890
Size: 223 284 513 367 5193
Fragments arranged by size:
5193 513 367 284 223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2610 2990 3174 3717 3775
Size: 325 217 627 823 380 184 543 58
Cuts at: 3775 3811 3901 5356 618
Size: 36 90 1455 1842
Fragments arranged by size:
1842 1455 823 627 543 380 325 217
184 90 58 36
BanII G_rGCy'C
Cuts at: 1391 2277 3639 3899 1391
Size: 886 1362 260 4072
Fragments arranged by size:
4072 1362 886 260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5609
BbvCI CC'TCA_GC
Cuts at: 2363 2363
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2900 2934 4771 4805 1903
Size: 34 963 34 1837 34 3678
Fragments arranged by size:
3678 1837 963 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3520 4472 5682 3520
Size: 952 1210 4418
Fragments arranged by size:
4418 1210 952
BclI T'GATC_A
Cuts at: 1548 2353 1548
Size: 805 5775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2418 5191 136
Size: 107 122 71 631 605 746 2773 1525
Fragments arranged by size:
2773 1525 746 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6575 6575
Size: 6580
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 2277 2442 2613 3780 4641 5797 1790
Size: 487 165 171 1167 861 1156 2573
Fragments arranged by size:
2573 1167 1156 861 487 171 165
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4311 5269 442
Size: 416 53 83 399 24 2894 958 1753
Fragments arranged by size:
2894 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 2591 2764 3081 3629 5240 1239
Size: 1320 32 173 317 548 1611 2579
Fragments arranged by size:
2579 1611 1320 548 317 173 32
Bpu10I CC'TnA_GC
Cuts at: 2363 2363
Size: 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2906 4705 5570 5868 2906
Size: 1799 865 298 3618
Fragments arranged by size:
3618 1799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2391 5243 882
Size: 617 892 2852 2219
Fragments arranged by size:
2852 2219 892 617
BsaAI yAC'GTr
Cuts at: 493 1204 3283 3860 3877 4371 493
Size: 711 2079 577 17 494 2702
Fragments arranged by size:
2702 2079 711 577 494 17
BsaBI GATnn'nnATC
Cuts at: 6574 6574
Size: 6580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2294 3237 4387 4769
Size: 53 83 186 936 761 943 1150 382
Cuts at: 4769 5404 275
Size: 635 1451
Fragments arranged by size:
1451 1150 943 936 761 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2180 2526 3870 5013 5411 5685 5832 6243 2180
Size: 346 1344 1143 398 274 147 411 2517
Fragments arranged by size:
2517 1344 1143 411 398 346 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3829 3859 1178
Size: 30 165 30 2426 30 3899
Fragments arranged by size:
3899 2426 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2188 3647 1186
Size: 1002 1459 4119
Fragments arranged by size:
4119 1459 1002
BseYI C'CCAG_C
Cuts at: 2270 3333 3728 3793 5783 6279 2270
Size: 1063 395 65 1990 496 2571
Fragments arranged by size:
2571 1990 1063 496 395 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3709 3737 1163
Size: 573 1973 28 4006
Fragments arranged by size:
4006 1973 573 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2442 2799 3639 4641 4726 5797 2442
Size: 357 840 1002 85 1071 3225
Fragments arranged by size:
3225 1071 1002 840 357 85
BsmI GAATG_Cn'
Cuts at: 4091 4184 4091
Size: 93 6487
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 2974 2285
Size: 689 5891
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2442 2613 2799 3639 3780 3899
Size: 399 487 165 171 186 840 141 119
Cuts at: 3899 4641 4726 5797 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 840 742 487 399 186 171
165 141 119 85
BspEI T'CCGG_A
Cuts at: 2526 6243 2526
Size: 3717 2863
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3799 3935 844
Size: 2955 136 3489
Fragments arranged by size:
3489 2955 136
BsrBI CCG'CTC
Cuts at: 1394 1823 4467 1394
Size: 429 2644 3507
Fragments arranged by size:
3507 2644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3828 5075 5249 65
Size: 1183 2580 1247 174 1396
Fragments arranged by size:
2580 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3087 3769 5224 5411 1700
Size: 110 1277 682 1455 187 2869
Fragments arranged by size:
2869 1455 1277 682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3283 794
Size: 2489 4091
BstBI TT'CG_AA
Cuts at: 2007 5384 2007
Size: 3377 3203
BstEII G'GTnAC_C
Cuts at: 1184 3562 1184
Size: 2378 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 2147 2338 2768 3860 2147
Size: 191 430 1092 4867
Fragments arranged by size:
4867 1092 430 191
BstYI r'GATC_y
Cuts at: 1972 2890 3113 3397 3910 4277 4662 4679 6131
Size: 918 223 284 513 367 385 17 1452
Cuts at: 6131 6217 6229 6575 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 918 513 385 367 346 284
223 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2416 2618 3723 513
Size: 552 957 394 202 1105 3370
Fragments arranged by size:
3370 1105 957 552 394 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 2440 3048 3138 3277 3671 3697 3764 4096 4890
Size: 608 90 139 394 26 67 332 794
Cuts at: 4890 4910 2440
Size: 20 4110
Fragments arranged by size:
4110 794 608 394 332 139 90 67
26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DraIII CAC_nnn'GTG
Cuts at: 3724 3724
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3158 5587 809
Size: 507 1842 2429 1802
Fragments arranged by size:
2429 1842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2621 3486
Size: 54 1425 50 275 18 195 596 865
Cuts at: 3486 4027 4916 6545 8
Size: 541 889 1629 43
Fragments arranged by size:
1629 1425 889 865 596 541 275 195
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3033 3647 4510 1353
Size: 1680 614 863 3423
Fragments arranged by size:
3423 1680 863 614
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3637 3637
Size: 6580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 2591 2764 2847 2922 3081 3629
Size: 393 927 32 173 83 75 159 548
Cuts at: 3629 4643 5240 6027 6295 1239
Size: 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 927 787 597 548 393 268
173 159 83 75 32
EcoNI CCTnn'n_nnAGG
Cuts at: 2939 2939
Size: 6580
EcoO109I rG'GnC_Cy
Cuts at: 2274 2274
Size: 6580
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 2328 2658 5727 1678
Size: 650 330 3069 2531
Fragments arranged by size:
3069 2531 650 330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2954 2986 3518 3550 3829
Size: 32 135 32 1382 32 532 32 279
Cuts at: 3829 3861 5221 5253 5295 5327 1373
Size: 32 1360 32 42 32 2626
Fragments arranged by size:
2626 1382 1360 532 279 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3924 4170 669
Size: 988 281 1986 246 3079
Fragments arranged by size:
3079 1986 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6580
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 2994 3178 3905 2994
Size: 184 727 5669
Fragments arranged by size:
5669 727 184
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 5694 5878 1305
Size: 921 3468 184 2007
Fragments arranged by size:
3468 2007 921 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3488 10
Size: 54 1425 343 1656 3102
Fragments arranged by size:
3102 1656 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2407 4538 4897 5056 518
Size: 648 1241 2131 359 159 2042
Fragments arranged by size:
2131 2042 1241 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2583 3337 3685 3797 3996 4673 5821 6066
Size: 977 754 348 112 199 677 1148 245
Cuts at: 6066 1606
Size: 2120
Fragments arranged by size:
2120 1148 977 754 677 348 245 199
112
NaeI GCC'GGC
Cuts at: 3089 3089
Size: 6580
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5071
Fragments arranged by size:
5071 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NgoMIV G'CCGG_C
Cuts at: 3087 3087
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 2509 2690 3085 3938 5483 1132
Size: 97 1280 181 395 853 1545 2229
Fragments arranged by size:
2229 1545 1280 853 395 181 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 2505 3081 5479 2505
Size: 576 2398 3606
Fragments arranged by size:
3606 2398 576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3484 1108
Size: 2376 4204
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PmlI CAC'GTG
Cuts at: 3860 3860
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 3300 4150 3300
Size: 850 5730
PspOMI G'GGCC_C
Cuts at: 2273 2273
Size: 6580
PspXI vC'TCGA_Gb
Cuts at: 2260 2260
Size: 6580
PstI C_TGCA'G
Cuts at: 830 3055 3133 3588 3932 830
Size: 2225 78 455 344 3478
Fragments arranged by size:
3478 2225 455 344 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 2583 3337 3685 3797 2583
Size: 754 348 112 5366
Fragments arranged by size:
5366 754 348 112
SacI G_AGCT'C
Cuts at: 3639 3639
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SexAI A'CCwGG_T
Cuts at: 3177 3177
Size: 6580
SfcI C'TryA_G
Cuts at: 826 1045 3001 3051 3129 3138 3584 3928 5063
Size: 219 1956 50 78 9 446 344 1135
Cuts at: 5063 5744 5935 826
Size: 681 191 1471
Fragments arranged by size:
1956 1471 1135 681 446 344 219 191
78 50 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2260 2486 2885 4684 5585 5847
Size: 197 926 317 226 399 1799 901 262
Cuts at: 5847 820
Size: 1553
Fragments arranged by size:
1799 1553 926 901 399 317 262 226
197
SnaBI TAC'GTA
Cuts at: 493 3877 4371 493
Size: 3384 494 2702
Fragments arranged by size:
3384 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6580
StyI C'CwwG_G
Cuts at: 513 1065 2022 2812 3258 3393 4019 513
Size: 552 957 790 446 135 626 3074
Fragments arranged by size:
3074 957 790 626 552 446 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3347 3449 3807 4607 4624 4777 4962
Size: 1255 1767 102 358 800 17 153 185
Cuts at: 4962 5407 325
Size: 445 1498
Fragments arranged by size:
1767 1498 1255 800 445 358 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2636 2679 4826
Size: 274 80 33 51 1443 659 43 2147
Cuts at: 4826 6538 96
Size: 1712 138
Fragments arranged by size:
2147 1712 1443 659 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3459 3491 6540 6572 3459
Size: 32 3049 32 3467
Fragments arranged by size:
3467 3049 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2966 1316
Size: 1650 4930
XbaI T'CTAG_A
Cuts at: 2452 3916 2452
Size: 1464 5116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2057 3193 3801 2057
Size: 1136 608 4836
Fragments arranged by size:
4836 1136 608
XhoI C'TCGA_G
Cuts at: 1943 2260 2486 1943
Size: 317 226 6037
Fragments arranged by size:
6037 317 226
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 4709 4709
Size: 6580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2295 4388 276
Size: 53 83 186 936 761 2093 2468
Fragments arranged by size:
2468 2093 936 761 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PpuMI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI