Restriction map for
pFN21ASDA1490
(Circular) (Six-base) MAPSORT of: pFN21ASDA1490.seq Check: 3767 from: 1 to: 6973
pFN21ASDA1490 6973 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4226 4226
Size: 6973
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4783 278
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4316 1937
Size: 2379 4594
Acc65I G'GTAC_C
Cuts at: 4294 4294
Size: 6973
AclI AA'CG_TT
Cuts at: 1563 5100 5473 1563
Size: 3537 373 3063
Fragments arranged by size:
3537 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2071 2598 2602 2882 5036 6420 6688 1632
Size: 439 527 4 280 2154 1384 268 1917
Fragments arranged by size:
2154 1917 1384 527 439 280 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6776
AflIII A'CryG_T
Cuts at: 1203 3906 3953 5872 6849 1203
Size: 2703 47 1919 977 1327
Fragments arranged by size:
2703 1919 1327 977 47
AgeI A'CCGG_T
Cuts at: 5804 5804
Size: 6973
AhdI GACnn_n'nnGTC
Cuts at: 5702 5702
Size: 6973
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3527 1184
Size: 2343 4630
AlwNI CAG_nnn'CTG
Cuts at: 2033 4716 6288 2033
Size: 2683 1572 2718
Fragments arranged by size:
2718 2683 1572
ApaLI G'TGCA_C
Cuts at: 5030 6186 5030
Size: 1156 5817
ApoI r'AATT_y
Cuts at: 1493 2582 2970 4282 4508 4660 6769 1493
Size: 1089 388 1312 226 152 2109 1697
Fragments arranged by size:
2109 1697 1312 1089 388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5527 160
Size: 872 4495 1606
Fragments arranged by size:
4495 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
AvaI C'yCGr_G
Cuts at: 1870 1943 4298 1870
Size: 73 2355 4545
Fragments arranged by size:
4545 2355 73
BamHI G'GATC_C
Cuts at: 3480 3742 4303 4670 3480
Size: 262 561 367 5783
Fragments arranged by size:
5783 561 367 262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2410 4238 4294 5749
Size: 325 217 627 516 107 1828 56 1455
Cuts at: 5749 618
Size: 1842
Fragments arranged by size:
1842 1828 1455 627 516 325 217 107
56
BanII G_rGCy'C
Cuts at: 1391 2344 2466 3096 4292 1391
Size: 953 122 630 1196 4072
Fragments arranged by size:
4072 1196 953 630 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6002
BbvCI CC'TCA_GC
Cuts at: 3087 3087
Size: 6973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2714 2748 5164 5198 1903
Size: 34 777 34 2416 34 3678
Fragments arranged by size:
3678 2416 777 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4865 6075 4865
Size: 1210 5763
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6973
BfrBI ATG'CAT
Cuts at: 3000 3155 3275 3000
Size: 155 120 6698
Fragments arranged by size:
6698 155 120
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3708 5584 136
Size: 107 122 71 631 605 2036 1876 1525
Fragments arranged by size:
2036 1876 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6968 6968
Size: 6973
BlpI GC'TnA_GC
Cuts at: 2607 4390 2607
Size: 1783 5190
Bme1580I G_kGCm'C
Cuts at: 1790 2357 5034 6190 1790
Size: 567 2677 1156 2573
Fragments arranged by size:
2677 2573 1156 567
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 4704 5662
Size: 416 53 83 399 24 759 2528 958
Cuts at: 5662 442
Size: 1753
Fragments arranged by size:
2528 1753 958 759 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2551 3733 5633 1239
Size: 1312 1182 1900 2579
Fragments arranged by size:
2579 1900 1312 1182
Bpu10I CC'TnA_GC
Cuts at: 3087 3203 3087
Size: 116 6857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2976 5098 5963 6261 2976
Size: 2122 865 298 3688
Fragments arranged by size:
3688 2122 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5636 882
Size: 617 4137 2219
Fragments arranged by size:
4137 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2386 4270 4764 493
Size: 711 1182 1884 494 2702
Fragments arranged by size:
2702 1884 1182 711 494
BsaBI GATnn'nnATC
Cuts at: 6967 6967
Size: 6973
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4780 5162 5797 275
Size: 53 83 186 936 3247 382 635 1451
Fragments arranged by size:
3247 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5406 5804 6078 6225 6636 5406
Size: 398 274 147 411 5743
Fragments arranged by size:
5743 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3922 3952 4222 4252 1178
Size: 30 165 30 2519 30 270 30 3899
Fragments arranged by size:
3899 2519 270 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 2269 2414 2417 2789 3104 3943 1186
Size: 1047 36 145 3 372 315 839 4216
Fragments arranged by size:
4216 1047 839 372 315 145 36 3
BseYI C'CCAG_C
Cuts at: 2174 6176 6672 2174
Size: 4002 496 2475
Fragments arranged by size:
4002 2475 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2679 1163
Size: 573 943 5457
Fragments arranged by size:
5457 943 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4423 5184 6212 1540
Size: 275 186 2422 761 1028 2301
Fragments arranged by size:
2422 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 2222 2466 3096 3797 3916 5034 5119 6190
Size: 56 244 630 701 119 1118 85 1071
Cuts at: 6190 2166
Size: 2949
Fragments arranged by size:
2949 1118 1071 701 630 244 119 85
56
BsmI GAATG_Cn'
Cuts at: 4484 4577 4484
Size: 93 6880
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2103
Size: 6973
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2222 2344 2357 2466 3096 3797
Size: 399 376 56 122 13 109 630 701
Cuts at: 3797 3916 4292 5034 5119 6190 1391
Size: 119 376 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 701 630 399 376 376
122 119 109 85 56 13
BspEI T'CCGG_A
Cuts at: 6636 6636
Size: 6973
BspHI T'CATG_A
Cuts at: 2937 3985 4862 6592 2937
Size: 1048 877 1730 3318
Fragments arranged by size:
3318 1730 1048 877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2701 4226 4328 844
Size: 1857 1525 102 3489
Fragments arranged by size:
3489 1857 1525 102
BsrBI CCG'CTC
Cuts at: 1394 1823 4860 1394
Size: 429 3037 3507
Fragments arranged by size:
3507 3037 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3202 3327 5468 5642 65
Size: 1183 1954 125 2141 174 1396
Fragments arranged by size:
2141 1954 1396 1183 174 125
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 2531 5617 5804 1700
Size: 110 298 423 3086 187 2869
Fragments arranged by size:
3086 2869 423 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6973
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6973
BssSI C'ACGA_G
Cuts at: 2323 5033 6045 2323
Size: 2710 1012 3251
Fragments arranged by size:
3251 2710 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2065 794
Size: 1271 5702
BstBI TT'CG_AA
Cuts at: 2007 5777 2007
Size: 3770 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6973
BstYI r'GATC_y
Cuts at: 1972 3480 3742 4303 4670 5055 5072 6524 6610
Size: 1508 262 561 367 385 17 1452 86
Cuts at: 6610 6622 6968 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1508 1452 561 385 367 346 262
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4065 513
Size: 552 3000 3421
Fragments arranged by size:
3421 3000 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6234
Fragments arranged by size:
6234 683 56
BtsI GCAGTG_nn'
Cuts at: 2155 3233 4489 5283 5303 2155
Size: 1078 1256 794 20 3825
Fragments arranged by size:
3825 1256 1078 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6973
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3234 3269 529
Size: 35 2670 35 4233
Fragments arranged by size:
4233 2670 35 35
DraI TTT'AAA
Cuts at: 4277 4277
Size: 6973
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5980 809
Size: 507 4664 1802
Fragments arranged by size:
4664 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 2736 3108
Size: 54 1425 50 275 18 703 203 372
Cuts at: 3108 3948 4420 5309 6938 8
Size: 840 472 889 1629 43
Fragments arranged by size:
1629 1425 889 840 703 472 372 275
203 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4420 1537
Size: 275 2608 4090
Fragments arranged by size:
4090 2608 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2251 2257 2278 2287 2384 4903 1353
Size: 737 161 6 21 9 97 2519 3423
Fragments arranged by size:
3423 2519 737 161 97 21 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5560 5934 6080 387
Size: 1064 138 3971 374 146 1280
Fragments arranged by size:
3971 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2464 3094 2464
Size: 630 6343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2551 2598 2602 2882 3733 5036
Size: 393 439 480 47 4 280 851 1303
Cuts at: 5036 5633 6420 6688 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1303 851 787 597 480 439 393
280 268 47 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2138
Size: 6973
EcoO109I rG'GnC_Cy
Cuts at: 2171 2815 3680 2171
Size: 644 865 5464
Fragments arranged by size:
5464 865 644
EcoRI G'AATT_C
Cuts at: 2582 2970 4282 4660 2582
Size: 388 1312 378 4895
Fragments arranged by size:
4895 1312 388 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3369 3369
Size: 6973
FspI TGC'GCA
Cuts at: 5479 5479
Size: 6973
HaeII r_GCGC'y
Cuts at: 1678 6120 1678
Size: 4442 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2549 2581 3457 3489 3924
Size: 32 135 32 977 32 876 32 435
Cuts at: 3924 3956 5614 5646 5688 5720 1373
Size: 32 1658 32 42 32 2626
Fragments arranged by size:
2626 1658 977 876 435 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3755 4317 4563 669
Size: 988 281 1704 113 562 246 3079
Fragments arranged by size:
3079 1704 988 562 281 246 113
HindIII A'AGCT_T
Cuts at: 748 2942 748
Size: 2194 4779
HpaI GTT'AAC
Cuts at: 4563 4563
Size: 6973
KpnI G_GTAC'C
Cuts at: 4298 4298
Size: 6973
MfeI C'AATT_G
Cuts at: 3783 4572 3783
Size: 789 6184
MluI A'CGCG_T
Cuts at: 6849 6849
Size: 6973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 3376 3817 3922 6087 6271 1305
Size: 1443 628 441 105 2165 184 2007
Fragments arranged by size:
2165 2007 1443 628 441 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3110 3950 10
Size: 54 1425 343 1278 840 3033
Fragments arranged by size:
3033 1425 1278 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3651 4931 5290 5449 518
Size: 648 2485 1280 359 159 2042
Fragments arranged by size:
2485 2042 1280 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2296 3086 4389 5066 6214 6459 1606
Size: 470 220 790 1303 677 1148 245 2120
Fragments arranged by size:
2120 1303 1148 790 677 470 245 220
NcoI C'CATG_G
Cuts at: 513 1065 4065 513
Size: 552 3000 3421
Fragments arranged by size:
3421 3000 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6973
NotI GC'GGCC_GC
Cuts at: 4420 4420
Size: 6973
NsiI A_TGCA'T
Cuts at: 3002 3157 3277 3002
Size: 155 120 6698
Fragments arranged by size:
6698 155 120
NspI r_CATG'y
Cuts at: 1132 1229 3184 3211 3910 3957 4331 5876 1132
Size: 97 1955 27 699 47 374 1545 2229
Fragments arranged by size:
2229 1955 1545 699 374 97 47 27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6973
PciI A'CATG_T
Cuts at: 3906 3953 5872 3906
Size: 47 1919 5007
Fragments arranged by size:
5007 1919 47
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3618 1108
Size: 2510 4463
PfoI T'CCnGG_A
Cuts at: 2428 2428
Size: 6973
PmeI GTTT'AAAC
Cuts at: 4277 4277
Size: 6973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5042 5074 6555 6587 5042
Size: 32 1481 32 5428
Fragments arranged by size:
5428 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2171 2815 2171
Size: 644 6329
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6973
PsiI TTA'TAA
Cuts at: 3224 4543 3224
Size: 1319 5654
PstI C_TGCA'G
Cuts at: 830 2334 2887 3516 4325 830
Size: 1504 553 629 809 3478
Fragments arranged by size:
3478 1504 809 629 553
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6973
PvuII CAG'CTG
Cuts at: 3086 3086
Size: 6973
SacI G_AGCT'C
Cuts at: 2466 3096 2466
Size: 630 6343
SalI G'TCGA_C
Cuts at: 1936 4315 1936
Size: 2379 4594
SanDI GG'GwC_CC
Cuts at: 2171 2171
Size: 6973
SbfI CC_TGCA'GG
Cuts at: 4325 4325
Size: 6973
ScaI AGT'ACT
Cuts at: 3672 5221 6933 3672
Size: 1549 1712 3712
Fragments arranged by size:
3712 1712 1549
SfcI C'TryA_G
Cuts at: 826 1045 2330 2883 3390 3411 3447 3512 4321
Size: 219 1285 553 507 21 36 65 809
Cuts at: 4321 5456 6137 6328 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1285 1135 809 681 553 507 219
191 65 36 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6973
SgrAI Cr'CCGG_yG
Cuts at: 1700 2108 1700
Size: 408 6565
SmaI CCC'GGG
Cuts at: 1872 4300 1872
Size: 2428 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2991 5077 5978 6240 820
Size: 197 926 1048 2086 901 262 1553
Fragments arranged by size:
2086 1553 1048 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4270 4764 493
Size: 3777 494 2702
Fragments arranged by size:
3777 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6973
SphI G_CATG'C
Cuts at: 1132 3211 4331 1132
Size: 2079 1120 3774
Fragments arranged by size:
3774 2079 1120
SspI AAT'ATT
Cuts at: 5 52 3878 4225 4897 5
Size: 47 3826 347 672 2081
Fragments arranged by size:
3826 2081 672 347 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6973
StyI C'CwwG_G
Cuts at: 513 1065 4065 4114 4412 513
Size: 552 3000 49 298 3074
Fragments arranged by size:
3074 3000 552 298 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5000 5017 5170 5355 5800 325
Size: 1255 3420 17 153 185 445 1498
Fragments arranged by size:
3420 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3670 5219 6931
Size: 274 80 33 51 1443 1693 1549 1712
Cuts at: 6931 96
Size: 138
Fragments arranged by size:
1712 1693 1549 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 3895 3922 3927 3954 4222 4254 6933
Size: 32 1567 27 5 27 268 32 2679
Cuts at: 6933 6965 2296
Size: 32 2304
Fragments arranged by size:
2679 2304 1567 268 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 1316 2819 3654 1316
Size: 1503 835 4635
Fragments arranged by size:
4635 1503 835
XbaI T'CTAG_A
Cuts at: 3313 4309 3313
Size: 996 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6973
XmaI C'CCGG_G
Cuts at: 1870 4298 1870
Size: 2428 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 5102 2084
Size: 3018 3955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4781 276
Size: 53 83 186 936 3247 2468
Fragments arranged by size:
3247 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI