Restriction map for
pFN21ASDA1473
(Circular) (Six-base) MAPSORT of: pFN21ASDA1473.seq Check: 6156 from: 1 to: 6322
pFN21ASDA1473 6322 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4385 5769 6037 1632
Size: 2753 1384 268 1917
Fragments arranged by size:
2753 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 5221 6198 1203
Size: 4018 977 1327
Fragments arranged by size:
4018 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 4065 5637 4065
Size: 1572 4750
ApaLI G'TGCA_C
Cuts at: 2300 4379 5535 2300
Size: 2079 1156 3087
Fragments arranged by size:
3087 2079 1156
ApoI r'AATT_y
Cuts at: 1493 2254 2393 2401 2505 2589 2757 2942 3009
Size: 761 139 8 104 84 168 185 67
Cuts at: 3009 3093 3110 3177 3631 3857 4009 6118 1493
Size: 84 17 67 454 226 152 2109 1697
Fragments arranged by size:
2109 1697 761 454 226 185 168 152
139 104 84 84 67 67 17 8
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 3647 1870
Size: 73 229 1475 4545
Fragments arranged by size:
4545 1475 229 73
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3643 5098 618
Size: 325 217 627 1856 1455 1842
Fragments arranged by size:
1856 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3641 1391
Size: 2250 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 928
Size: 971 142 5209
Fragments arranged by size:
5209 971 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 2082 1548
Size: 534 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4933 136
Size: 107 122 71 631 605 3261 1525
Fragments arranged by size:
3261 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2304 4383 5539 1790
Size: 514 2079 1156 2573
Fragments arranged by size:
2573 2079 1156 514
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2144 4053 5011
Size: 416 53 83 399 24 727 1909 958
Cuts at: 5011 442
Size: 1753
Fragments arranged by size:
1909 1753 958 727 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3548
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2787 2871 2955 3039 3123 3207 3291
Size: 875 673 84 84 84 84 84 84
Cuts at: 3291 3375 3459 3543 4982 1239
Size: 84 84 84 1439 2579
Fragments arranged by size:
2579 1439 875 673 84 84 84 84
84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4447 5312 5610 4447
Size: 865 298 5159
Fragments arranged by size:
5159 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2169 2555 4985 882
Size: 617 670 386 2430 2219
Fragments arranged by size:
2430 2219 670 617 386
BsaAI yAC'GTr
Cuts at: 493 1204 3619 4113 493
Size: 711 2415 494 2702
Fragments arranged by size:
2702 2415 711 494
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4129 4511 5146 275
Size: 53 83 186 936 2596 382 635 1451
Fragments arranged by size:
2596 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4755 5153 5427 5574 5985 4755
Size: 398 274 147 411 5092
Fragments arranged by size:
5092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6097
Fragments arranged by size:
6097 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 3478 1186
Size: 1534 758 4030
Fragments arranged by size:
4030 1534 758
BseYI C'CCAG_C
Cuts at: 5525 6021 5525
Size: 496 5826
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2814 1163
Size: 573 1078 4671
Fragments arranged by size:
4671 1078 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 4383 4468 5539 2304
Size: 2079 85 1071 3087
Fragments arranged by size:
3087 2079 1071 85
BsmI GAATG_Cn'
Cuts at: 3833 3926 3833
Size: 93 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 3641 4383 4468 5539 1391
Size: 399 514 1337 742 85 1071 2174
Fragments arranged by size:
2174 1337 1071 742 514 399 85
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 4211 5941 4211
Size: 1730 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3677 844
Size: 2833 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4209 1394
Size: 429 2386 3507
Fragments arranged by size:
3507 2386 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3570 4817 4991 65
Size: 1183 2322 1247 174 1396
Fragments arranged by size:
2322 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2643 3147 4966 5153 1700
Size: 110 833 504 1819 187 2869
Fragments arranged by size:
2869 1819 833 504 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 2007 5126 2007
Size: 3119 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 3652 4019 4404 4421 5873 5959 5971 6317
Size: 1680 367 385 17 1452 86 12 346
Cuts at: 6317 1972
Size: 1977
Fragments arranged by size:
1977 1680 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 3838 4632 4652 3838
Size: 794 20 5508
Fragments arranged by size:
5508 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3061 3626 3061
Size: 565 5757
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2183 3769 4658
Size: 54 1425 50 275 18 353 1586 889
Cuts at: 4658 6287 8
Size: 1629 43
Fragments arranged by size:
1629 1586 1425 889 353 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2616 4252 1353
Size: 764 499 1636 3423
Fragments arranged by size:
3423 1636 764 499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4909 5283 5429 387
Size: 1064 138 3320 374 146 1280
Fragments arranged by size:
3320 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2787 2871 2955 3039 3123 3207
Size: 393 482 673 84 84 84 84 84
Cuts at: 3207 3291 3375 3459 3543 4385 4982 5769 6037
Size: 84 84 84 84 842 597 787 268
Cuts at: 6037 1239
Size: 1524
Fragments arranged by size:
1524 842 787 673 597 482 393 268
84 84 84 84 84 84 84 84
84
EcoRI G'AATT_C
Cuts at: 2505 2589 2757 3009 3093 3177 3631 4009 2505
Size: 84 168 252 84 84 454 378 4818
Fragments arranged by size:
4818 454 378 252 168 84 84 84
FspI TGC'GCA
Cuts at: 4828 4828
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 5469 1678
Size: 3791 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 4963 4995 5037
Size: 32 135 32 502 32 2857 32 42
Cuts at: 5037 5069 1373
Size: 32 2626
Fragments arranged by size:
2857 2626 502 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2718 2886 3054 3222 3306 3390 748
Size: 1970 168 168 168 84 84 3680
Fragments arranged by size:
3680 1970 168 168 168 84 84
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5436 5620 1305
Size: 4131 184 2007
Fragments arranged by size:
4131 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2185 10
Size: 54 1425 343 353 4147
Fragments arranged by size:
4147 1425 353 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4280 4639 4798 518
Size: 648 3114 359 159 2042
Fragments arranged by size:
3114 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3738 4415 5563 5808 1606
Size: 2132 677 1148 245 2120
Fragments arranged by size:
2132 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5770
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6322
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2479 3680 5225 1132
Size: 97 1250 1201 1545 2229
Fragments arranged by size:
2229 1545 1250 1201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6322
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PstI C_TGCA'G
Cuts at: 830 3674 830
Size: 2844 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SexAI A'CCwGG_T
Cuts at: 2043 2043
Size: 6322
SfcI C'TryA_G
Cuts at: 826 1045 2035 3670 4805 5486 5677 826
Size: 219 990 1635 1135 681 191 1471
Fragments arranged by size:
1635 1471 1135 990 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4426 5327 5589 820
Size: 197 926 2483 901 262 1553
Fragments arranged by size:
2483 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3619 4113 493
Size: 3126 494 2702
Fragments arranged by size:
3126 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 2479 3680 1132
Size: 1347 1201 3774
Fragments arranged by size:
3774 1347 1201
SspI AAT'ATT
Cuts at: 5 52 2358 4246 5
Size: 47 2306 1888 2081
Fragments arranged by size:
2306 2081 1888 47
StuI AGG'CCT
Cuts at: 1517 2972 1517
Size: 1455 4867
StyI C'CwwG_G
Cuts at: 513 1065 2111 3761 513
Size: 552 1046 1650 3074
Fragments arranged by size:
3074 1650 1046 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4349 4366 4519 4704 5149 325
Size: 1255 2769 17 153 185 445 1498
Fragments arranged by size:
2769 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4568 6280 96
Size: 274 80 33 51 1443 2591 1712 138
Fragments arranged by size:
2591 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6282 6314 6282
Size: 32 6290
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 2441 3013 4451 2441
Size: 572 1438 4312
Fragments arranged by size:
4312 1438 572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI