Restriction map for
pFN21ASDA1470
(Circular) (Six-base) MAPSORT of: pFN21ASDA1470.seq Check: 4325 from: 1 to: 6295
pFN21ASDA1470 6295 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3212 3212
Size: 6295
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4358 5742 6010 1632
Size: 2726 1384 268 1917
Fragments arranged by size:
2726 1917 1384 268
AfeI AGC'GCT
Cuts at: 2144 2144
Size: 6295
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 2747 2995 4038 5610 2747
Size: 248 1043 1572 3432
Fragments arranged by size:
3432 1572 1043 248
ApaI G_GGCC'C
Cuts at: 2097 2209 2439 2097
Size: 112 230 5953
Fragments arranged by size:
5953 230 112
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2425 2813 3315 3604 3830 3982 6091 1493
Size: 932 388 502 289 226 152 2109 1697
Fragments arranged by size:
2109 1697 932 502 388 289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2138 2285 2485 2910 3620 1870
Size: 73 125 70 147 200 425 710 4545
Fragments arranged by size:
4545 710 425 200 147 125 73 70
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2451 2859 3616 5071 618
Size: 325 217 627 664 408 757 1455 1842
Fragments arranged by size:
1842 1455 757 664 627 408 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2097 2199 2209 2439 2492 2687 3614
Size: 678 28 102 10 230 53 195 927
Cuts at: 3614 1391
Size: 4072
Fragments arranged by size:
4072 927 678 230 195 102 53 28
10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2981 3067 3426 928
Size: 971 1082 86 359 3797
Fragments arranged by size:
3797 1082 971 359 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 2807 3108
Size: 107 122 71 631 605 446 689 301
Cuts at: 3108 4906 136
Size: 1798 1525
Fragments arranged by size:
1798 1525 689 631 605 446 301 122
107 71
BglII A'GATC_T
Cuts at: 3186 6290 3186
Size: 3104 3191
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2209 2439 2864 2917 4356 5512 1790
Size: 307 112 230 425 53 1439 1156 2573
Fragments arranged by size:
2573 1439 1156 425 307 230 112 53
BmgBI CAC'GTC
Cuts at: 1318 3025 1318
Size: 1707 4588
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 3416 4026
Size: 416 53 83 399 24 954 1045 610
Cuts at: 4026 4984 442
Size: 958 1753
Fragments arranged by size:
1753 1045 958 954 610 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 3331 4955 1239
Size: 974 1118 1624 2579
Fragments arranged by size:
2579 1624 1118 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5285 5583 4420
Size: 865 298 5132
Fragments arranged by size:
5132 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2488 4958 882
Size: 617 989 2470 2219
Fragments arranged by size:
2470 2219 989 617
BsaAI yAC'GTr
Cuts at: 493 1204 3592 4086 493
Size: 711 2388 494 2702
Fragments arranged by size:
2702 2388 711 494
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2493 2523 2530 2560 3191
Size: 30 165 30 1090 30 7 30 631
Cuts at: 3191 3221 3491 3521 1178
Size: 30 270 30 3952
Fragments arranged by size:
3952 1090 631 270 165 30 30 30
30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2501 1186
Size: 886 429 4980
Fragments arranged by size:
4980 886 429
BseYI C'CCAG_C
Cuts at: 2541 2566 2722 2753 2878 3106 3378 5498 5994
Size: 25 156 31 125 228 272 2120 496
Cuts at: 5994 2541
Size: 2842
Fragments arranged by size:
2842 2120 496 272 228 156 125 31
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2484 3314 1163
Size: 573 748 830 4144
Fragments arranged by size:
4144 830 748 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2237 2268 3745 4506 5534 1540
Size: 275 186 236 31 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 236 186 31
BsiHKAI G_wGCw'C
Cuts at: 2069 2199 2292 3371 4356 4441 5512 2069
Size: 130 93 1079 985 85 1071 2852
Fragments arranged by size:
2852 1079 1071 985 130 93 85
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
BsmBI CGTCTCn'nnnn_
Cuts at: 3015 3015
Size: 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2097 2199 2209 2292 2439 2492
Size: 399 279 28 102 10 83 147 53
Cuts at: 2492 2687 2864 2917 3371 3614 4356 4441 5512
Size: 195 177 53 454 243 742 85 1071
Cuts at: 5512 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 454 399 279 243 195
177 147 102 85 83 53 53 28
10
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3212 3475 3650 844
Size: 2368 263 175 3489
Fragments arranged by size:
3489 2368 263 175
BsrBI CCG'CTC
Cuts at: 1394 1823 2134 4182 1394
Size: 429 311 2048 3507
Fragments arranged by size:
3507 2048 429 311
BsrDI GCAATG_nn'
Cuts at: 65 1248 4790 4964 65
Size: 1183 3542 174 1396
Fragments arranged by size:
3542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2236 3102 4939 5126 1700
Size: 110 426 866 1837 187 2869
Fragments arranged by size:
2869 1837 866 426 187 110
BsrGI T'GTAC_A
Cuts at: 96 2772 96
Size: 2676 3619
BssHII G'CGCG_C
Cuts at: 1925 2224 1925
Size: 299 5996
BssSI C'ACGA_G
Cuts at: 2491 4355 5367 2491
Size: 1864 1012 3419
Fragments arranged by size:
3419 1864 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6295
BstBI TT'CG_AA
Cuts at: 2007 5099 2007
Size: 3092 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 2447
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 3186 3625 3992 4377 4394 5846 5932 5944
Size: 1214 439 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1214 439 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2268 2520 3492 513
Size: 552 1203 252 972 3316
Fragments arranged by size:
3316 1203 972 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2890 487
Size: 683 56 1664 3892
Fragments arranged by size:
3892 1664 683 56
BtsI GCAGTG_nn'
Cuts at: 2079 3297 3438 3811 4605 4625 2079
Size: 1218 141 373 794 20 3749
Fragments arranged by size:
3749 1218 794 373 141 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 2629 2629
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2234 2265 2779
Size: 54 1425 50 275 18 404 31 514
Cuts at: 2779 2958 3100 3354 3742 4631 6260 8
Size: 179 142 254 388 889 1629 43
Fragments arranged by size:
1629 1425 889 514 404 388 275 254
179 142 54 50 43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 2234 2265 3742 1537
Size: 275 422 31 1477 4090
Fragments arranged by size:
4090 1477 422 275 31
EarI CTCTTCn'nnn_
Cuts at: 1353 3332 4225 1353
Size: 1979 893 3423
Fragments arranged by size:
3423 1979 893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2668 4882 5256 5402 387
Size: 1064 138 1079 2214 374 146 1280
Fragments arranged by size:
2214 1280 1079 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2067 2197 2067
Size: 130 6165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 3331 4358 4955 5742 6010 1239
Size: 393 581 1118 1027 597 787 268 1524
Fragments arranged by size:
1524 1118 1027 787 597 581 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2205 2206 2435 2436 3298 2205
Size: 1 229 1 862 5202
Fragments arranged by size:
5202 862 229 1 1
EcoRI G'AATT_C
Cuts at: 2813 3315 3604 3982 2813
Size: 502 289 378 5126
Fragments arranged by size:
5126 502 378 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2354 3091 2354
Size: 737 5558
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 2146 3039 5442 1678
Size: 468 893 2403 2531
Fragments arranged by size:
2531 2403 893 468
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2530 2562 2946 2978 4936
Size: 32 135 32 958 32 384 32 1958
Cuts at: 4936 4968 5010 5042 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1958 958 384 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 10
Size: 54 1425 343 1128 3345
Fragments arranged by size:
3345 1425 1128 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 3350 4253 4612 4771 518
Size: 648 1279 905 903 359 159 2042
Fragments arranged by size:
2042 1279 905 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2175 2566 2722 2878 2920 3378 3711
Size: 554 15 391 156 156 42 458 333
Cuts at: 3711 4388 5536 5781 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 554 458 391 333 245
156 156 42 15
NaeI GCC'GGC
Cuts at: 2238 3104 2238
Size: 866 5429
NcoI C'CATG_G
Cuts at: 513 1065 3492 513
Size: 552 2427 3316
Fragments arranged by size:
3316 2427 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NgoMIV G'CCGG_C
Cuts at: 2236 3102 2236
Size: 866 5429
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2649 3653 5198 1132
Size: 97 1420 1004 1545 2229
Fragments arranged by size:
2229 1545 1420 1004 97
PasI CC'CwG_GG
Cuts at: 1348 3167 1348
Size: 1819 4476
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2665 3049 3378 1108
Size: 1557 384 329 4025
Fragments arranged by size:
4025 1557 384 329
PfoI T'CCnGG_A
Cuts at: 3054 3054
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3298 3298
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 2480 1613
Size: 867 5428
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspOMI G'GGCC_C
Cuts at: 2093 2205 2435 2093
Size: 112 230 5953
Fragments arranged by size:
5953 230 112
PstI C_TGCA'G
Cuts at: 830 2150 2644 3445 3647 830
Size: 1320 494 801 202 3478
Fragments arranged by size:
3478 1320 801 494 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2566 2722 2878 3378 2566
Size: 156 156 500 5483
Fragments arranged by size:
5483 500 156 156
SacI G_AGCT'C
Cuts at: 2069 2199 2069
Size: 130 6165
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SapI GCTCTTCn'nnn_
Cuts at: 3332 3332
Size: 6295
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2146 2640 3441 3643 4778 5459 5650
Size: 219 1101 494 801 202 1135 681 191
Cuts at: 5650 826
Size: 1471
Fragments arranged by size:
1471 1135 1101 801 681 494 219 202
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3108 3108
Size: 6295
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 2912 3622 1872
Size: 1040 710 4545
Fragments arranged by size:
4545 1040 710
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4399 5300 5562 820
Size: 197 926 2456 901 262 1553
Fragments arranged by size:
2456 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3592 4086 493
Size: 3099 494 2702
Fragments arranged by size:
3099 2702 494
SpeI A'CTAG_T
Cuts at: 152 2941 152
Size: 2789 3506
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SrfI GCCC'GGGC
Cuts at: 2912 2912
Size: 6295
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 2295 2664 2728 3492 3510 3734 513
Size: 552 1230 369 64 764 18 224 3074
Fragments arranged by size:
3074 1230 764 552 369 224 64 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 2439 2847 3218 4322 4339 4492
Size: 1255 828 31 408 371 1104 17 153
Cuts at: 4492 4677 5122 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1104 828 445 408 371 185
153 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 4541 6253
Size: 274 80 33 51 1443 795 1769 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
1769 1712 1443 795 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2493 2525 6255 6287 2493
Size: 32 3730 32 2501
Fragments arranged by size:
3730 2501 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3191 3191
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 2910 3620 1870
Size: 1040 710 4545
Fragments arranged by size:
4545 1040 710
XmnI GAAnn'nnTTC
Cuts at: 2943 4424 2943
Size: 1481 4814
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfoI SwaI